Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	149,567	T→C	Y12C (TAC→TGC)	panB ←	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	149,567	0	T	C	100.0%	155.7 / NA	45	Y12C (TAC→TGC)	panB	3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (18/27);  total (18/27)

GTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGA  >  NC_000913/149432‑149698                                                                                                                                        |                                                                                                                                    gTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACtt                                                                                                                                 <  2:870624/140‑1 (MQ=255) gTGGAGTCGTGCCCCTGAACCGTCAGGCCGAGCGAATAGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACtt                                                                                                                                 <  1:443845/140‑1 (MQ=255)    gAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTg                                                                                                                              <  2:1009705/140‑1 (MQ=255)    gAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTg                                                                                                                              <  1:615173/140‑1 (MQ=255)    gAGTCGTGCCCCTGAACCGTCAAGCCCAGCGGATCCCCCTCCAGCATGTCGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTg                                                                                                                              <  2:268448/140‑1 (MQ=255)       tCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAg                                                                                                                           <  1:805032/140‑1 (MQ=255)       tCGTGCCCCTGAACCGTCATGCCCACCGACTCGCCCAGCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAg                                                                                                                           <  1:415175/140‑1 (MQ=255)          tGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTc                                                                                                                                <  2:781348/132‑1 (MQ=255)          tGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTc                                                                                                                                >  1:781348/1‑132 (MQ=255)            ccccTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAgg                                                                                                                      >  1:140187/1‑140 (MQ=255)              ccTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGgag                                                                                                                    <  2:815645/140‑1 (MQ=255)                      gTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATggtggt                                                                                                            <  2:837367/140‑1 (MQ=255)                       tCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTc                                                                                                           >  1:686064/1‑140 (MQ=255)                             ccAGCGAATCGCCCACCAGCATTACGTTAAGCCATGCATCAGCAAAGATTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc                                                                                                     <  2:440508/140‑1 (MQ=255)                             ccAGCGAAGCGCCCACGCGCATCACGTTAAGTCCTGCATAAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTc                                                                                                     <  2:455959/140‑1 (MQ=255)                                gCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATa                                                                                                  <  2:808833/140‑1 (MQ=255)                                 cGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTTCACTTCTGCAGTAAGGAGATGCTGGTCGGTTTCATaa                                                                                                 >  1:352867/1‑140 (MQ=255)                                 cGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATaa                                                                                                 >  2:75850/1‑140 (MQ=255)                                     tCGCCCACCAGCATGTCGTTAAACCGTTCTTCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTa                                                                                             <  1:359436/140‑1 (MQ=255)                                     tCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTa                                                                                             <  2:675405/140‑1 (MQ=255)                                      cGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTAt                                                                                            <  2:746566/140‑1 (MQ=255)                                             caGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATa                                                                                     <  2:140187/140‑1 (MQ=255)                                                  tGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTACAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt                                                                                  <  1:869777/138‑1 (MQ=255)                                                  tGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTACAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt                                                                                  >  2:869777/1‑138 (MQ=255)                                                          aGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt                                                                                  >  2:802152/1‑130 (MQ=255)                                                          aGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAAt                                                                                  <  1:802152/130‑1 (MQ=255)                                                          aGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTtgtg                                                                        >  1:2337/1‑140 (MQ=255)                                                                tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGTGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTCTCATAACGTATGCTAATAAATTGATGTTGTGCTGTCt                                                                  >  1:274796/1‑140 (MQ=255)                                                                tcatcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                  >  2:902805/1‑140 (MQ=255)                                                                 catcaGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCGTGATAAATTCATGTTGTGCTGTCTg                                                                 >  1:467810/1‑140 (MQ=255)                                                                    caGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATACATTGATGTTGTGCTGTCTGGCa                                                              >  1:416863/1‑140 (MQ=255)                                                                       cAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                  <  2:925919/133‑1 (MQ=255)                                                                       cAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCt                                                                  >  1:925919/1‑133 (MQ=255)                                                                               ttGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTc                                                   >  1:980241/1‑140 (MQ=255)                                                                                  gCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTcaca                                                <  1:85094/140‑1 (MQ=255)                                                                                   cGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACAt                                               >  2:699730/1‑140 (MQ=255)                                                                                       gCTATAGTCATAAGCGGGGATGTTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATtggt                                           <  2:352867/140‑1 (MQ=255)                                                                                                aTAAGCGGTGATGGTCGCGAAATGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAAt                                  >  2:548252/1‑140 (MQ=255)                                                                                                  aaGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCTGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGa                                >  2:652633/1‑140 (MQ=255)                                                                                                        tGATGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATc                          <  2:108836/140‑1 (MQ=255)                                                                                                        tGATGGTCCCGAAACGATTTTTCTCCTGTTCGCACGTCTGCAGTAAGGGGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATc                          <  2:467810/140‑1 (MQ=255)                                                                                                           tGGTCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGt                       <  2:790403/140‑1 (MQ=255)                                                                                                              tCGCGAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagc                    <  1:902805/140‑1 (MQ=255)                                                                                                               cgcgAAACGTTTTTTTTCCTGTTTGCACTTCTGCAGTAAGGAGATGGTGGGCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTagca                   >  2:558776/1‑140 (MQ=255)                                                                                                                                ccTGTTTGCACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACAATGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGa  <  2:2337/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    GTGGAGTCGTGCCCCTGAACCGTCATGCCCAGCGAATCGCCCACCAGCATGACGTTAAGCCCTTCATCAGCAAAGAGTTTGGCGAAGCTATAGTCATAAGCGGTGATGGTCGCGAAACGTTTTTTTTCCTGTTTGTACTTCTGCAGTAAGGAGATGGTGGTCGGTTTCATAACGTATCCTGATAAATTGATGTTGTGCTGTCTGGCATTTTATCAGTCACATTGGTGGGGGCAATGATTTATCCGTAGCAGCACTGCCAGAAGGTGA  >  NC_000913/149432‑149698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑