Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,524	T→C	T129A (ACT→GCT)	marC ←	inner membrane protein MarC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,524	0	T	C	100.0%	127.8 / NA	36	T129A (ACT→GCT)	marC	inner membrane protein MarC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/12);  total (24/12)

TTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGAC  >  NC_000913/1618389‑1618659                                                                                                                                        |                                                                                                                                        ttCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACtt                                                                                                                                     >  1:453974/1‑140 (MQ=255)    cccACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAAGAATCATCGCTAGGGATCCCGGACCGGCAGCACTTGGc                                                                                                                                  >  1:300202/1‑140 (MQ=255)       acaAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCAtt                                                                                                                               <  2:453974/140‑1 (MQ=255)                   ccaccaGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGcaca                                                                                                                   <  2:5907/140‑1 (MQ=255)                    caccaGGAAAAAGATCAGCGGCAGCGCAACCATCAGTCCCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACaa                                                                                                                  <  1:468555/140‑1 (MQ=255)                            aaaaGATCAGCGGCGGCACAACCATCAGTACTCAAACAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAata                                                                                                          <  2:300202/140‑1 (MQ=255)                                         cggcgCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGtt                                                                                             >  1:36301/1‑140 (MQ=255)                                           gcgcAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTTCAAACGGCACAACGGCAAAATTGGCACTGGGTTCa                                                                                           >  2:450125/1‑140 (MQ=255)                                                   acaggTACCCAATCAGCGAAGGATGCACTCTGACGCAGTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAg                                                                                   <  1:475777/136‑1 (MQ=255)                                                       gTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGctct                                                                               <  1:601314/140‑1 (MQ=255)                                                          cccAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTcc                                                                            >  2:906119/1‑140 (MQ=255)                                                             aaTCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGAc                                                                         >  2:41660/1‑140 (MQ=255)                                                             aaTCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGAAATATTGTCACCGGGTTCATCTTCCAGCTCTTCCGAc                                                                         >  1:421265/1‑140 (MQ=255)                                                              aTCAGCGAAGGTTGAACTCTGAAGCGCTGCGGACGCCAAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACt                                                                        <  1:450125/140‑1 (MQ=255)                                                               tCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAGTATTGGCTCTGAGTGCATCTTGCAGCTCTTCCGACtt                                                                       >  1:279904/1‑140 (MQ=255)                                                                  gCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCt                                                                    >  2:687705/1‑140 (MQ=255)                                                                  gCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCt                                                                    >  2:568245/1‑140 (MQ=255)                                                                  gCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAACATTGGAACTGGGTTCAACTTCCAGCTCTTCCGACtctgt                                                                    >  2:475777/1‑136 (MQ=255)                                                                      aGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTc                                                                >  1:552698/1‑140 (MQ=255)                                                                               tctGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGt                                                       <  1:41660/140‑1 (MQ=255)                                                                                   aCGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCAGTGGGTTCATCTTCCAGATCTTCCGAATTGCTTTTCGCCTCCGGTGaat                                                   >  2:371829/1‑140 (MQ=255)                                                                                   aCGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGaat                                                   <  2:915843/140‑1 (MQ=255)                                                                                         tgtgGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTGCATCTTCCTGCTCTTATGACTTGCTTTTCGCCTCCGGTGAATCAATCg                                             >  2:368004/1‑140 (MQ=255)                                                                                           tgGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCt                                           >  2:675019/1‑140 (MQ=255)                                                                                             gACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCttt                                         <  1:152230/140‑1 (MQ=255)                                                                                                     gCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGt                                     >  1:826934/1‑136 (MQ=255)                                                                                                     gCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGt                                     <  2:826934/136‑1 (MQ=255)                                                                                                     gCTAATAATCATCGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCg                                 >  1:41526/1‑140 (MQ=255)                                                                                                            atcatcGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAagag                          <  1:117898/140‑1 (MQ=255)                                                                                                               gtcGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGGCTCAGGTAAATCAATCGCTTTTTGTTGCGGAAAGAGCAt                       >  2:269801/2‑140 (MQ=255)                                                                                                                tcGCTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATc                      >  2:512060/1‑140 (MQ=255)                                                                                                                   cTATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGa                   >  1:756025/1‑140 (MQ=255)                                                                                                                    tATGGTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGaa                  >  1:193422/1‑140 (MQ=255)                                                                                                                       ggTTCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAAc               >  2:844650/1‑140 (MQ=255)                                                                                                                         ttCCCGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCg             >  2:695178/1‑140 (MQ=255)                                                                                                                            ccGGACCGGCAGCACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAAGCGCTTTCTGTTGCGTAAAGAGCATCCGAAAACCGATa          >  1:426765/1‑140 (MQ=255)                                                                                                                                    gcagcaCTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGcgac  >  1:159592/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        TTCCCCACAAAATAACCGCCACCAGGAAAAAGATCAGCGGCGGCGCAACCATCAGTACCCAATCAGCGAAGGTTGAACTCTGACGCACTGTGGACGCCGAGCTAATAATCATCGCTATGGTTCCCGGACCGGCAGTACTTGGCATTGCCAGCGGCACAAAGGCAATATTGGCACTGGGTTCATCTTCCAGCTCTTCCGACTTGCTTTTCGCCTCCGGTGAATCAATCGCTTTCTGTTGCGGAAAGAGCATCCGAAAACCGATAAACGCGAC  >  NC_000913/1618389‑1618659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑