Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,670,408	A→G	D237G (GAC→GGC)	ynfM →	putative transporter YnfM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,670,408	0	A	G	100.0%	154.8 / NA	46	D237G (GAC→GGC)	ynfM	putative transporter YnfM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (21/25);  total (21/25)

CTGGCGGCAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGACCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCT  >  NC_000913/1670275‑1670533                                                                                                                                      |                                                                                                                              ctGGCGGCAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCgggg                                                                                                                         <  2:952880/140‑1 (MQ=255)   ggcggcAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGAt                                                                                                                       <  1:810551/140‑1 (MQ=255)   ggcggcAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGAt                                                                                                                       <  2:911270/140‑1 (MQ=255)   ggcggcAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGAt                                                                                                                       <  2:912574/140‑1 (MQ=255)   ggcggcAATCGGTTGTCACGCGCTGGCCTCGCCTTTGATTTTCTGGAAGATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGAt                                                                                                                       <  2:467921/140‑1 (MQ=255)        cAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCg                                                                                                                  <  1:1200/140‑1 (MQ=255)              gttgttTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGcc                                                                                                                             >  1:993747/1‑123 (MQ=255)              gttgttTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTg                                                                                                            >  2:735469/1‑140 (MQ=255)                       cgcTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTc                                                                                                         >  1:51849/1‑134 (MQ=255)                       cgcTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTc                                                                                                         <  2:51849/134‑1 (MQ=255)                         cTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAgg                                                                                                 <  2:909494/140‑1 (MQ=255)                                cGGCGTTGATGTTCTGGAAAATACTACCTGAATCACGGCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTg                                                                                          <  1:376948/140‑1 (MQ=255)                                 ggCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGc                                                                                         >  2:568923/1‑140 (MQ=255)                                      tGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAAAAGGCTTTTTGCTGATg                                                                                    >  1:638925/1‑140 (MQ=255)                                       gATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATgg                                                                                   >  2:91513/1‑140 (MQ=255)                                             cTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGtcgt                                                                             >  2:215114/1‑140 (MQ=255)                                             cTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGtcgt                                                                             <  2:938810/140‑1 (MQ=255)                                                   aaTCCTCCCTGAATCCCGCCATCTTTGCCTGACGTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGTGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCa                                                                       <  1:261059/140‑1 (MQ=255)                                                    aTCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCAc                                                                      <  2:951742/140‑1 (MQ=255)                                                     tcctccCTGAATCACGCTATTTGCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACg                                                                     <  2:413184/140‑1 (MQ=255)                                                     tcctccCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACg                                                                     <  1:828839/140‑1 (MQ=255)                                                                   cGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACAt                                                       >  1:112809/1‑140 (MQ=255)                                                                   cGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACAt                                                       <  1:189305/140‑1 (MQ=255)                                                                    gCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCa                                                                       <  2:628117/123‑1 (MQ=255)                                                                    gCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCa                                                                       >  1:628117/1‑123 (MQ=255)                                                                    gCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATc                                                      >  1:975853/1‑140 (MQ=255)                                                                    gCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTCTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATc                                                      >  1:608589/1‑140 (MQ=255)                                                                       aTTTTCGCCCGACTTCGCTGCGACCTAAGACCTTGATTACCAACTTTCTTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGc                                                   <  2:336294/140‑1 (MQ=255)                                                                        ttttCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCt                                                  >  1:82068/1‑140 (MQ=255)                                                                                     tCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGct                                     <  2:975853/140‑1 (MQ=255)                                                                                         tGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTcc                                 >  2:698035/1‑140 (MQ=255)                                                                                          gcgcCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTccc                                <  1:215114/140‑1 (MQ=255)                                                                                             cccTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCt                             >  2:175247/1‑140 (MQ=255)                                                                                                        tgttTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATgtcagt                  >  2:701668/1‑140 (MQ=255)                                                                                                        tgttTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATgtcagt                  >  1:529781/1‑140 (MQ=255)                                                                                                        tgttTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATgtcagt                  <  1:2551/140‑1 (MQ=255)                                                                                                        tgttTATCAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACAGCGGCTATGGGTTGACGCTCTCGCCCTGGCATgtcagt                  >  1:295983/1‑140 (MQ=255)                                                                                                               cAACTTTCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCg           >  1:179794/1‑140 (MQ=255)                                                                                                                   tttCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGt       <  2:608589/140‑1 (MQ=255)                                                                                                                   tttCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGt       <  2:112809/140‑1 (MQ=255)                                                                                                                    ttCGTCTGCGCTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAgg              <  2:904579/132‑1 (MQ=255)                                                                                                                    ttCGTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAgg              >  1:904579/1‑132 (MQ=255)                                                                                                                      cGTCTGCACTGGCGTGGCCGGGAATGACCTTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTgg    <  1:448371/140‑1 (MQ=255)                                                                                                                       gTCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGc   <  1:91513/140‑1 (MQ=255)                                                                                                                        tCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCt  >  2:252030/1‑140 (MQ=255)                                                                                                                        tCTGCACTGGCGTGGCCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCt  >  1:183227/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                              CTGGCGGCAATCGGTTGTTTCGCGCTGGCCTCGGCGTTGATGTTCTGGAAAATCCTCCCTGAATCACGCCATTTTCGCCCGACTTCGCTGCGCCCTAAGACGTTGTTTATCAACTTTCGTCTGCACTGGCGTGACCGGGGATTACCGTTATTGTTCGCAGAAGGCTTTTTGCTGATGGGGTCGTTCGTCACGCTGTTTAATTACATCGGCTATCGGTTGATGCTCTCCCCCTGGCATGTCAGTCAGGCCGTGGTTGGCT  >  NC_000913/1670275‑1670533

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑