Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,711,998	T→C	V159A (GTA→GCA)	nth →	endonuclease III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,711,998	0	T	C	98.6%	234.3 / NA	69	V159A (GTA→GCA)	nth	endonuclease III
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (32/36);  total (32/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGTAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGTATTATTGAAGATCTTTGTGAA  >  NC_000913/1711870‑1712134                                                                                                                                 |                                                                                                                                         cGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGc                                                                                                                               >  1:644102/1‑140 (MQ=255)    aGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTAc                                                                                                                            <  1:574226/140‑1 (MQ=255)      gTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTg                                                                                                                          >  2:100645/1‑140 (MQ=255)          tAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAg                                                                                                                      >  2:161475/1‑140 (MQ=255)          tAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGAGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAg                                                                                                                      >  2:315868/1‑140 (MQ=255)           aaaaCAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGt                                                                                                                     <  2:619006/140‑1 (MQ=255)              aCAGCCAACGTCGTATTCAACACTGCATTCATCGGGCCGTCTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGt                                                                                                                  <  2:491935/140‑1 (MQ=255)                aGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTc                                                                                                                >  1:144395/1‑140 (MQ=255)                aGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTc                                                                                                                <  1:762306/140‑1 (MQ=255)                aGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGATc                                                                                                                <  2:644102/140‑1 (MQ=255)                  ccAACGTCGTATTAAACACTGCCTTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCca                                                                                                              >  2:257011/1‑140 (MQ=255)                  ccAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTc                                                                                                                <  2:816156/138‑1 (MQ=255)                  ccAACGTCGCATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTc                                                                                                                >  1:816156/1‑138 (MQ=255)                      cgtcgtATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCcagcag                                                                                                          <  1:257011/140‑1 (MQ=255)                       gtcgtATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAGAAACGTCGGACAAGCATAAGAAAAGGTACTAAAAGTGGTTCCAGCaga                                                                                                         >  2:261517/1‑140 (MQ=255)                          gtATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAG‑AAAGCTACTGAAAGTGGTTCcagca                                                                                                           >  2:139682/1‑134 (MQ=255)                          gtATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAG‑AAAGCTACTGAAAGTGGTTCcagca                                                                                                           <  1:139682/134‑1 (MQ=255)                           tATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGttt                                                                                                     >  2:524809/1‑140 (MQ=255)                                aaCACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGt                                                                                                >  2:829713/1‑140 (MQ=255)                                   acTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGa                                                                                             <  1:737750/140‑1 (MQ=255)                                     tGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACt                                                                                           >  2:999814/1‑140 (MQ=255)                                     tGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACt                                                                                           >  2:786878/1‑140 (MQ=255)                                      gCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTg                                                                                          >  2:47971/1‑140 (MQ=255)                                      gCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTg                                                                                          >  2:7470/1‑140 (MQ=255)                                      gCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTg                                                                                          >  2:827466/1‑140 (MQ=255)                                       cATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCcagca                                                                                                           <  1:782830/122‑1 (MQ=255)                                       cATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCcagca                                                                                                           >  2:782830/1‑122 (MQ=255)                                       cATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGc                                                                                         >  2:77843/1‑140 (MQ=255)                                       cATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCCACTGAAAGTGGGTCTAGCAGAGTTTAAAGTCGACTGc                                                                                         >  1:372145/1‑140 (MQ=255)                                        aTTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGcc                                                                                        <  1:786878/140‑1 (MQ=255)                                           cGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGccacc                                                                                     >  1:106269/1‑140 (MQ=255)                                            ggCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGccacca                                                                                    >  1:602569/1‑140 (MQ=255)                                               tGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGccacca                                                                                    <  1:829003/137‑1 (MQ=255)                                               tGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGccacca                                                                                    >  2:829003/1‑137 (MQ=255)                                                 gCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGt                                                                               >  1:985449/1‑140 (MQ=255)                                                 gCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGt                                                                               <  2:106269/140‑1 (MQ=255)                                                      cTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATc                                                                          <  1:827466/140‑1 (MQ=255)                                                           gCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCATAGTTTAAGGTCAACCGCCACCATTGGTTGATCCTGCa                                                                     >  1:328560/1‑140 (MQ=255)                                                           gCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCa                                                                     <  1:899623/140‑1 (MQ=255)                                                           gCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCa                                                                     <  2:115984/140‑1 (MQ=255)                                                           gCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCa                                                                     <  2:232064/140‑1 (MQ=255)                                                            cTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCAc                                                                    <  1:77843/140‑1 (MQ=255)                                                                cGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGc                                                                >  2:96932/1‑140 (MQ=255)                                                                  acacGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGt                                                              <  2:985449/140‑1 (MQ=255)                                                                               ccGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTg                                                 <  2:724661/140‑1 (MQ=255)                                                                               ccGCGTTTGTAATCGTACTCAACTTGCGCCGCGGAAAAATGTCGAACACGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTg                                                 <  2:372145/140‑1 (MQ=255)                                                                                      tGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCaa                                          >  1:622215/1‑140 (MQ=255)                                                                                       gTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAg                                         <  1:224265/140‑1 (MQ=255)                                                                                        tAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGc                                        <  2:144395/140‑1 (MQ=255)                                                                                         aaTCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCAAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGcc                                       <  1:923411/140‑1 (MQ=255)                                                                                             gTACTCAATTTGCGCTGGGGAACAAGGTCCAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGc                                   <  1:261517/140‑1 (MQ=255)                                                                                             gTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGc                                   <  1:829713/140‑1 (MQ=255)                                                                                               aCTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATTCCTGCATTGCCCGCAAGCCCCGCtg                                 >  1:463058/1‑140 (MQ=255)                                                                                               aCTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTATAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCtg                                 <  2:636219/140‑1 (MQ=255)                                                                                                cTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCtgt                                >  1:569346/1‑140 (MQ=255)                                                                                                cTCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCtgt                                <  2:635272/140‑1 (MQ=255)                                                                                                 tCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCtgtg                               >  2:815389/1‑140 (MQ=255)                                                                                                 tCAATTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCtgtg                               >  2:91728/1‑140 (MQ=255)                                                                                                   aaTTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGc                             <  2:569346/140‑1 (MQ=255)                                                                                                   aaTTTGCGCCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGc                             <  1:100645/140‑1 (MQ=255)                                                                                                         cgcCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGt                       >  1:843392/1‑140 (MQ=255)                                                                                                          gcCGGGGAAAAACGTCGAACAGTCACAAGTAAAGCTATTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGta                      <  1:315868/140‑1 (MQ=255)                                                                                                          gcCGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGta                      <  1:161475/140‑1 (MQ=255)                                                                                                           gccgggAAAAACGTCTAACAGGCAGAAGATAAGCTACCGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGtat                     <  1:327837/137‑1 (MQ=255)                                                                                                           ccGGGGAAAAACGTCGGCCAGGTAGAAGAAAGGCTTCTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGtat                     <  2:463058/140‑1 (MQ=255)                                                                                                           ccGGGGAAAAACGTCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGtat                     <  1:518173/140‑1 (MQ=255)                                                                                                                        tCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGTattatt                 <  2:733652/131‑1 (MQ=255)                                                                                                                        tCGAACAGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGTattatt                 >  1:733652/1‑131 (MQ=255)                                                                                                                              aGGCAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGTATTATTGAAGATCTTTGTGaa  <  2:552588/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                         CGTAGGTCGTAAAACAGCCAACGTCGTATTAAACACTGCATTCGGCTGGCCGACTATTGCTGTCGACACGCACATTTTCCGCGTTTGTAATCGTACTCAATTTGCGCCGGGGAAAAACGTCGAACAGGTAGAAGAAAAGCTACTGAAAGTGGTTCCAGCAGAGTTTAAAGTCGACTGCCACCATTGGTTGATCCTGCACGGGCGTTATACCTGCATTGCCCGCAAGCCCCGCTGTGGCTCTTGTATTATTGAAGATCTTTGTGAA  >  NC_000913/1711870‑1712134

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑