Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,776,375	A→G	A263A (GCA→GCG)	ydiF →	putative acetate‑CoA transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,776,375	0	A	G	100.0%	137.1 / NA	42	A263A (GCA→GCG)	ydiF	putative acetate‑CoA transferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/22);  total (20/22)

GTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCG  >  NC_000913/1776250‑1776511                                                                                                                              |                                                                                                                                         gtgATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCtt                                                                                                                            <  2:96629/140‑1 (MQ=255)    atgatgCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTAt                                                                                                                         >  2:223274/1‑140 (MQ=255)    atgatgCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATCCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTAt                                                                                                                         >  2:804242/1‑140 (MQ=255)     tgatgCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTAtt                                                                                                                        <  1:556133/140‑1 (MQ=255)       atgCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTc                                                                                                                      >  1:960802/1‑140 (MQ=255)         gCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTg                                                                                                                    >  2:707929/1‑140 (MQ=255)         gCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTg                                                                                                                    >  1:167647/1‑140 (MQ=255)            ggTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTg                                                                                                                 >  2:587000/1‑140 (MQ=255)                cAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAAGCCAACTGTATGGGGGTGCGCCGGTTGACCGATTTATTTCTGGTGACtt                                                                                                             >  2:260077/1‑140 (MQ=255)                   aaaaTGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCAGTTCACCAGTTGACTGCCTTACTTCCGGCGACTTCAc                                                                                                          >  1:479060/1‑140 (MQ=255)                        ggTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTtg                                                                                                     <  1:77522/140‑1 (MQ=255)                         gTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTtga                                                                                                    <  1:940838/140‑1 (MQ=255)                          ttAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTtgat                                                                                                   <  1:888320/140‑1 (MQ=255)                           tAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTtgatg                                                                                                  >  2:756665/1‑140 (MQ=255)                             aGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGAc                                                                                                >  2:611166/1‑140 (MQ=255)                              gAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACa                                                                                               <  1:129742/140‑1 (MQ=255)                                  gCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTAc                                                                                           >  2:570334/1‑140 (MQ=255)                                  gCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTAc                                                                                           <  2:1016545/140‑1 (MQ=255)                                   ccACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCAGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTAcc                                                                                          <  1:857057/140‑1 (MQ=255)                                             tCCTAAATCTGTCCGTATTCCGGGTTATCGGGTGGATTTTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGcc                                                                                <  1:488060/140‑1 (MQ=255)                                              ccTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCt                                                                               <  2:126913/140‑1 (MQ=255)                                               cTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTg                                                                              <  1:587000/140‑1 (MQ=255)                                                  aaTCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCATGATGACAGTACCAAACTTAGCCTGccc                                                                           >  1:1012307/1‑140 (MQ=255)                                                        tCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCAGCCTTGATCACAATACCAAACTTAGCCTGCCCCTAAAc                                                                     >  1:321166/1‑140 (MQ=255)                                                                    gTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTa                                                         >  2:778463/1‑140 (MQ=255)                                                                       aTCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGtt                                                      >  1:721984/1‑140 (MQ=255)                                                                              ggATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgcggc                                               <  1:223274/140‑1 (MQ=255)                                                                              ggATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgcggc                                               <  1:804242/140‑1 (MQ=255)                                                                                 tatTGTGGTGGTCGATCGGGATGAAACCCAAGTGTATGGCGGTGCGCCTGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgcggcgcg                                            <  2:321166/140‑1 (MQ=255)                                                                                  atTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCTAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGgcgcgc                                           <  2:960802/140‑1 (MQ=255)                                                                                  atTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCATGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGgcgcgc                                           <  2:1012307/140‑1 (MQ=255)                                                                                    tgtgGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgcggc                                               <  1:884408/134‑1 (MQ=255)                                                                                    tgtgGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGAGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTgcgcggc                                               >  2:884408/1‑134 (MQ=255)                                                                                      tggtggTCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCatta                                       >  1:73913/1‑140 (MQ=255)                                                                                           gtCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGa                                  >  1:128622/1‑140 (MQ=255)                                                                                            tCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGaa                                 <  1:218914/140‑1 (MQ=255)                                                                                            tCGATCCGGATCAAACCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGaa                                 <  2:607753/140‑1 (MQ=255)                                                                                                      tCAAACCCAACTGTACGGCGGTACGACGGCTAACCGCCTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAg                       <  1:260077/140‑1 (MQ=255)                                                                                                          aCCCAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGgcgc                   >  2:519155/1‑140 (MQ=255)                                                                                                           cccAACTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGcgcg                  <  2:167647/140‑1 (MQ=255)                                                                                                               aCTGTATGGCGGTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGCGCGGTgg              >  2:728189/1‑140 (MQ=255)                                                                                                                      ggCGGTGCGCCGGTTAATCGCTTTATTTCTGGTGACTTCGCCCTTGATAACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGt       <  2:286070/140‑1 (MQ=255)                                                                                                                      ggCGGTGCACCGGTTAATCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAACCCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGt       <  2:1013526/140‑1 (MQ=255)                                                                                                                          gTGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATCATTCGAATTGCATATAGGCGCTGTGGGGAATGTCGGc   >  1:307300/1‑140 (MQ=255)                                                                                                                           tGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAAGGCGGAAGGGCGCGCTGGGGAATGTCGGCg  >  1:305177/1‑140 (MQ=255)                                                                                                                           tGCGCCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGGAAATTAGTTGCGCGGCGCGCATTTTTCGAAAAGCGGAAAGGCGCGGTGGGGAATGTCGGCg  >  1:413061/1‑140 (MQ=255)                                                                                                                              |                                                                                                                                         GTGATGATGCAGGTGCAGAAAATGGTTAAGAAAGCCACGCTGCATCCTAAATCTGTCCGTATTCCGGGTTATCTGGTGGATATTGTGGTGGTCGATCCGGATCAAACCCAACTGTATGGCGGTGCACCGGTTAACCGCTTTATTTCTGGTGACTTCACCCTTGATGACAGTACCAAACTTAGCCTGCCCCTAAACCAACGTAAATTAGTTGCGCGGCGCGCATTATTCGAAATGCGTAAAGGCGCGGTGGGGAATGTCGGCG  >  NC_000913/1776250‑1776511

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑