Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,792,782 T→C intergenic (‑51/+27) nlpC ← / ← btuD NlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,792,7820TC96.2% 82.6 / NA 26intergenic (‑51/+27)nlpC/btuDNlpC/P60 family lipoprotein NlpC/vitamin B12 ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (17/8);  total (18/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCA  >  NC_000913/1792646‑1792917
                                                                                                                                        |                                                                                                                                       
gAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCAcc                                                                                                                                      <  1:720127/140‑1 (MQ=255)
 aaaGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGCTTTTTATGCTTGCAGTAATTTAGCGTCGTAATCAccc                                                                                                                                     >  1:280975/1‑140 (MQ=255)
                                 tggCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTc                                                                                                     >  1:534459/1‑140 (MQ=255)
                                   gCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGa                                                                                                   >  2:631405/1‑140 (MQ=255)
                                   gCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAACGTCGTAAACACGCGACTTTCAAGATACTATTGAAATCACATGGTCGa                                                                                                   >  2:462228/1‑140 (MQ=255)
                                          ccGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCATAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGc                                                                                            <  2:79822/140‑1 (MQ=255)
                                             gCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTATCAAGATACTAATGAAGTCAGATGGTCGAAATCAGCAtt                                                                                         >  2:339517/1‑140 (MQ=255)
                                             gCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAtt                                                                                         >  1:859601/1‑140 (MQ=255)
                                             gCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAtt                                                                                         >  2:43598/1‑140 (MQ=255)
                                             gCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTATGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCAtt                                                                                         >  2:825188/1‑140 (MQ=255)
                                                    gCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGAc                                                                                  <  1:703506/140‑1 (MQ=255)
                                                             tgATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGatat                                                                         >  2:862856/1‑140 (MQ=255)
                                                                         ggCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATTAGATGGTCGAAAGCAGGCTTCTGTGACCTTCGATATCCAGACGGCGaa                                                             >  2:482711/1‑140 (MQ=255)
                                                                         ggCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATGAGATGGTCAAAAACAGCATTCTGTGACCTTAGATATCCAGACGGCGaa                                                             >  2:478075/1‑140 (MQ=255)
                                                                               aaCGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCa                                                       <  2:679007/140‑1 (MQ=255)
                                                                               aaCGCATTCCGTACGGTTCCTAGGTTTTTCATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCa                                                       <  1:368516/140‑1 (MQ=255)
                                                                                         gTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGc                                             <  1:892512/140‑1 (MQ=255)
                                                                                         gTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGc                                             >  2:680489/1‑140 (MQ=255)
                                                                                          tACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGcc                                            >  2:769417/1‑140 (MQ=255)
                                                                                          tACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGcc                                            >  1:130740/1‑140 (MQ=255)
                                                                                                               tCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTg                       <  2:534459/140‑1 (MQ=255)
                                                                                                               tCTTGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAAATCAAGCCATAGGACTGCCACAGATTTGGCGGCGTg                       >  2:487816/1‑140 (MQ=255)
                                                                                                                 ttGCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAg                     <  2:809417/140‑1 (MQ=255)
                                                                                                                   gCATTAATTTAGCGTCGTAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCa                   <  1:862856/140‑1 (MQ=255)
                                                                                                                                   gtAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTcc   >  1:193979/1‑140 (MQ=255)
                                                                                                                                   gtAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTAGGCGGCGTGAGCAACTCTTCCCTGCGTcc   >  1:275853/1‑140 (MQ=255)
                                                                                                                                    tAATCACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCa  >  1:113360/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GAAAGTCTGGCATTTGGCGGCGGTGCTTTATGGTGGCTACACCCGGCCAGAAGCAGTGCTGTGATCAAAATAAGGCAGAAACGCATTCCGTACGGTTCCTCTGTTTTTTATTCTTGCATTAATTTAGCGTCGTAATTACCCGATTTTCAAGATACTAATGAAATCAGATGGTCGAAATCAGCATTCTGTGACCTTCGATATCCAGACGGCGAAAATTCATCCCATAGGCCTGCGCCAGATTTGGCGGCGTGAGCACCTCTTCCCTGCGTCCA  >  NC_000913/1792646‑1792917

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑