Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,606,329 (G)8→6 noncoding (72‑73/87 nt) leuQ ← tRNA‑Leu

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,606,3230G.98.8% 314.3 / ‑2.1 81noncoding (79/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (45/35);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
*NC_0009134,606,3240G.98.8% 313.4 / ‑3.0 81noncoding (78/87 nt)leuQtRNA‑Leu
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base . (45/35);  total (45/36)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 102 total aligned reads displayed.

ATATCGCCTCGTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606187‑4606456
                                                                                                                                          ||                                                                                                                                    
tatcgcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGATGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggg                                                                                                                                     <  2:1781147‑M2/118‑1 (MQ=255)
  tcgcctcgtttggtgcggggGGCG‑GGGGACTCGAACCCCTACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                                                   <  2:864975‑M2/120‑1 (MQ=255)
  tcgcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggg                                                                                                                                   <  1:1517398‑M2/120‑1 (MQ=255)
   tCGCCTCGTTTGGTGCGT‑GGGGAGGGGACTTGAACCCCCCCGTCCGTATGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                                                  <  2:956205/140‑1 (MQ=21)
   cgcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCTCCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                                                  <  1:76015‑M2/121‑1 (MQ=255)
   cgcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggg                                                                                                                                  <  1:1845942‑M2/121‑1 (MQ=255)
   cgcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCAACCTTCGCCCAGTGCGAGCAATATCAACGCGGTTTTTGGTGCGAgggggg                                                                                                                                  >  1:2477761‑M2/20‑140 (MQ=255)
    gcctcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAAGATCAACTTGGTTTGTGGTGCGagggggga                                                                                                                                 >  1:864975‑M2/19‑139 (MQ=255)
    cGCCTCGTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAgggggga                                                                                                                                 >  1:1236351/1‑139 (MQ=255)
     gCCTCGTTTGGTGCGAGGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAggggggac                                                                                                                                <  2:1236351/140‑3 (MQ=255)
       tcgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTgcgaggggggactt                                                                                                                              <  1:217938‑M2/125‑5 (MQ=255)
       ccgtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTgcgaggggggactt                                                                                                                              <  1:197167‑M2/125‑5 (MQ=255)
         gtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGgtgcgaggggggacttga                                                                                                                            >  1:427548‑M2/14‑134 (MQ=255)
         gtttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGgtgcgaggggggacttga                                                                                                                            <  1:1854833‑M2/127‑7 (MQ=255)
          tttggtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCTATATCAACGTGCTTGTTggtcagaggggggacttgac                                                                                                                           >  1:1170986‑M2/13‑133 (MQ=255)
              gtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAAcccc                                                                                                                     <  2:1494451‑M2/132‑1 (MQ=255)
              gtgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGGGCGAGCAAAATCTACGGGGTTTTTGGTGCGA‑‑GGGGGGACTTGAAcccc                                                                                                                     >  2:688770‑M2/9‑140 (MQ=255)
               tgcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAAccccc                                                                                                                    >  2:2379300‑M2/8‑140 (MQ=255)
                gcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCa                                                                                                                   >  1:1713616‑M2/7‑140 (MQ=255)
                gcgaggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCa                                                                                                                   <  1:2379300‑M2/134‑1 (MQ=255)
                  gcggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACg                                                                                                                 <  1:2027891‑M2/136‑1 (MQ=255)
                    ggGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTc                                                                                                               <  1:538805‑M2/138‑1 (MQ=255)
                    agGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTgcgaggggggactt                                                                                                                              <  1:1640818‑M2/125‑5 (MQ=255)
                    agGGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTgcgaggggggactt                                                                                                                              >  2:1640818‑M2/3‑123 (MQ=255)
                         g‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                            <  1:1919997/138‑1 (MQ=255)
                         g‑ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGt                                                                                                            >  2:1919997/1‑138 (MQ=255)
                           ggggACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAg                                                                                                         <  1:1402193/140‑1 (MQ=255)
                             ggACCTGAACCCCCAGGTGCGTAAAAACAATAACACCAGAAGGAAGCTCAACTACCAATGACGCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGa                                                                                                       <  2:1432551/140‑1 (MQ=255)
                              gACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGac                                                                                                      <  2:283028/140‑1 (MQ=255)
                               aCTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGCGCGA‑‑GGGTGGAGTTCAACCCCGACGTCCGTAAGGaca                                                                                                     >  1:1161345/1‑140 (MQ=21)
                                  tGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTa                                                                                                  >  1:283028/1‑140 (MQ=255)
                                   gAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTaa                                                                                                 <  2:1695686/140‑1 (MQ=255)
                                              tCCGTAAGAACACTAACACCTGAAGCTAGCGCGGCTGCCAATTCCGCCACCTTCGCCCAGTGCTAGCAATATCACCGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                                                                      <  2:2477761/140‑1 (MQ=37)
                                                cGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                                                                    <  2:427548/140‑1 (MQ=255)
                                                 gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                   >  2:1832842/1‑140 (MQ=255)
                                                 gTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                                                                   <  2:2069679/140‑1 (MQ=255)
                                                  tAAGAACACTAACTCCTGAAGCCAGCGCGTCTACCTATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                  <  2:729663/140‑1 (MQ=37)
                                                  tAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                                                                  <  1:2281806/140‑1 (MQ=255)
                                                       acacTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                                                             >  2:2131276/1‑140 (MQ=255)
                                                          cTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGG‑TTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCGGTAAGGACACTGACACCTGAAGCTAGCGCGTCTACCa                                                                         >  1:954587/1‑140 (MQ=37)
                                                            aaCACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                                                                        >  1:1439711/1‑140 (MQ=255)
                                                                ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                                                                    >  1:2460898/1‑140 (MQ=255)
                                                                ccTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTGCGAGCACTATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCAACATCCGTAAGGTCACAAACACCTGCAGCTGGCGCGTCTACCACTTcc                                                                    >  2:1009298/1‑140 (MQ=25)
                                                                  tGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:206776/140‑1 (MQ=255)
                                                                  tGAAGCTAGCGCGTCGACCAATACCCCCACCTTCGCCAAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                                                                  <  1:958749/140‑1 (MQ=21)
                                                                   gAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGAGACTAACACCTGGAGCTAGCGCATCTACCAATTCCGcc                                                                 >  1:821347/1‑140 (MQ=25)
                                                                    aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                <  1:1832842/140‑1 (MQ=255)
                                                                    aaGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCa                                                                >  1:1561490/1‑140 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTATCGCGTCTACTAATTCCGCCACCTTc                                                           >  1:1052330/1‑140 (MQ=25)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                         >  1:2476689/1‑126 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                         >  1:1300993/1‑126 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                         <  2:2476689/126‑1 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                                                                         <  2:1300993/126‑1 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                           >  2:392714/1‑140 (MQ=255)
                                                                         aGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                                                           >  1:1467874/1‑140 (MQ=255)
                                                                             cgTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCAt                                                       <  1:392714/140‑1 (MQ=255)
                                                                               tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTACCGCGTCTATCAATTTCGCCACCTTCGCATAc                                                     >  2:1096359/1‑140 (MQ=25)
                                                                               tCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCAGAACCTAGCGCGTCTAGCAATTCCGCCACCTTCGCATAc                                                     >  2:1008880/1‑140 (MQ=25)
                                                                                 tACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCa                                                   >  2:2039139/1‑140 (MQ=255)
                                                                                    cAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCa                                                >  2:1392481/1‑140 (MQ=255)
                                                                                     aaTTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCaa                                               <  1:2203410/140‑1 (MQ=255)
                                                                                        tCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTc                                            >  2:2016955/1‑140 (MQ=255)
                                                                                          cGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGGAGCTAGCGCGTCGACCAATTCCGCCACCTTGGCATACCATCAATTCtt                                          >  2:700273/1‑140 (MQ=25)
                                                                                            ccACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaa                                        >  1:2376776/1‑140 (MQ=255)
                                                                                             cACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTaaa                                       >  1:201537/1‑140 (MQ=255)
                                                                                                 ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                   <  1:1392481/140‑1 (MQ=255)
                                                                                                 ttCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGa                                   >  2:2126075/1‑140 (MQ=255)
                                                                                                  tCGCCCAGCGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGaa                                  >  1:170879/1‑140 (MQ=255)
                                                                                                    gCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAAtt                                >  1:1922600/1‑140 (MQ=255)
                                                                                                       cAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCt                             >  1:2212353/1‑140 (MQ=255)
                                                                                                         gTGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCTTAAGGAAACTACCACCTGAAGCTAGGGCGTCTACCAATTCCCCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAc                           >  1:1669632/1‑140 (MQ=25)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc                          >  1:1231106/1‑140 (MQ=255)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc                          >  1:496816/1‑140 (MQ=255)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTAcc                          >  1:2070421/1‑140 (MQ=255)
                                                                                                          tGCGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACAAATTCCGCGACCATCGTATACCATCGATTCTTAAAAAGAATTGCTAcc                          >  2:953314/1‑140 (MQ=21)
                                                                                                            cGAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCAc                        >  2:381541/1‑140 (MQ=255)
                                                                                                             gAGCAATATCAACGTTGTTTTTGGTTCGA‑‑AGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                       <  1:1008880/140‑1 (MQ=21)
                                                                                                             gAGCAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACg                       <  2:170879/140‑1 (MQ=255)
                                                                                                                cAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  2:496816/140‑1 (MQ=255)
                                                                                                                cAATATCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  2:2070421/140‑1 (MQ=255)
                                                                                                                cAATATCAACGTGGTTTCTGGTTCGA‑‑GGGGGGACTTGAAGCCCCACGTTCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAg                    <  2:1052330/140‑1 (MQ=21)
                                                                                                                   tatCAACGTGGTTTTTGGTGCGAGGTGGGG‑‑CTTGAACGCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  1:1096359/140‑1 (MQ=25)
                                                                                                                   tatCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  2:2460898/140‑1 (MQ=255)
                                                                                                                   aagcAACGTGGTTTTGGGTGC‑‑GGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  2:1795511/137‑1 (MQ=21)
                                                                                                                    atCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc                >  1:190649/1‑140 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc                >  1:937096/1‑140 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcgc                >  1:2371963/1‑140 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  1:2128058/139‑1 (MQ=255)
                                                                                                                    atCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 >  2:2128058/1‑139 (MQ=255)
                                                                                                                     tCAACGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAACTCTTAAAAAGAATTGCTACCACGGAGGCGCa               <  1:2016955/140‑1 (MQ=255)
                                                                                                                         cGTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTAGCATACCAACAAATCTGAAAAAGAAGTGCTGCCACGGAGGCGCATTCt           >  2:655417/1‑140 (MQ=18)
                                                                                                                          gTGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTa          <  2:1713616/140‑1 (MQ=255)
                                                                                                                           tGGTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCACTCATCCCTAAGGACATTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAg         <  2:1175533/140‑1 (MQ=25)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGTCTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg       >  2:516453/1‑140 (MQ=255)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTGTTAAAAACAATCGCGACCACGCAGGCGCATTCTAGTg       >  1:1175533/1‑140 (MQ=18)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 >  1:242071/1‑130 (MQ=255)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGgcg                 <  2:242071/130‑1 (MQ=255)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg       <  2:201537/140‑1 (MQ=255)
                                                                                                                             gTTTTTGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTg       >  2:2042588/1‑140 (MQ=255)
                                                                                                                                  tGGTGCGA‑‑GGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGtttt  >  1:476954/1‑140 (MQ=255)
                                                                                                                                          ||                                                                                                                                    
ATATCGCCTCGTTTGGTGCGA‑GGGG‑GGGGACTTGAACCCCCACGTCCGTAAGAACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCCCAGTGCGAGCAATATCAACGTGGTTTTTGGTGCGAGGGGGGGGACTTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCATACCATCAATTCTTAAAAAGAATTGCTACCACGGAGGCGCATTCTAGTGGTTTT  >  NC_000913/4606187‑4606456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑