Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,212,088	(C)8→7	intergenic (‑85/+172)	iraM ← / ← ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,212,081	0	C	.	100.0%	383.5 / NA	91	intergenic (‑78/+179)	iraM/ymgK	anti‑adaptor protein IraM, inhibitor of sigma(S) proteolysis/protein YmgK
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (50/41);  total (50/41)

CTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211946‑1212216                                                                                                                                        |                                                                                                                                        cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:2295274/1‑140 (MQ=255) cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:2435447/1‑140 (MQ=255) cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  2:380378/1‑140 (MQ=255) cTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccc                                                                                                                                     >  1:1991295/1‑140 (MQ=255)  tGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGATTATTTCATGAATTAGCATATGAGTATATTAcccccc                                                                                                                                    >  1:871077/1‑140 (MQ=255)   gAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTAccccccc                                                                                                                                   >  2:2292224/1‑140 (MQ=255)           tataCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCaaaaaa                                                                                                                          >  1:1393003/1‑140 (MQ=255)              aCCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAaaata                                                                                                                       >  2:2218518/1‑140 (MQ=255)                   aTGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaatta                                                                                                                  >  1:2006369/1‑140 (MQ=255)                      tAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAaattaatt                                                                                                               <  1:2044963/140‑1 (MQ=255)                       aGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAattaatta                                                                                                              >  2:1721000/1‑140 (MQ=255)                            gAATTACCGTGTCAATTGCTATCCACTTCAAGTATTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:1178016/140‑1 (MQ=255)                            gAATTACCGTGTCAATTCCTATACACTTCATCTGTTATGCCTTATCCCCCGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:982416/140‑1 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTTTATTA‑CCACCCTTCTAAAAAAAAAATTAATTAAAatg                                                                                                         >  1:1178016/1‑140 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:2006369/140‑1 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  1:2292224/140‑1 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:1662093/140‑1 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         >  1:1662093/1‑140 (MQ=255)                            gAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAatg                                                                                                         <  2:2288078/140‑1 (MQ=255)                             aaTTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTTCATATGAGCATAGTA‑CGCCCCCTCAAAAAACTAAATTAATTAAAatga                                                                                                        >  2:774317/1‑140 (MQ=255)                                      gtCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTtata                                                                                               <  1:380378/140‑1 (MQ=255)                                       tCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTGTATTA‑CCCACCCACAATAAAATAAATTAATCAAAATGATGGCTtatat                                                                                              >  2:870390/1‑140 (MQ=255)                                          aTTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATataaa                                                                                           >  1:2367889/1‑140 (MQ=255)                                                 tCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATaaaataaaat                                                                                    >  2:1710693/1‑140 (MQ=255)                                                   cACTTCATTTGTTATGTATTATACCACGGTTTTTAATATTGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAAttt                                                                                  <  2:1154593/140‑1 (MQ=255)                                                    aCTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTa                                                                                 >  2:2461003/1‑140 (MQ=255)                                                      ttCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTaaa                                                                               >  1:1732954/1‑140 (MQ=255)                                                       tCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAg                                                                              <  2:1732954/140‑1 (MQ=255)                                                         aTTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCa                                                                            <  1:265178/140‑1 (MQ=255)                                                            tGTTATGTCTTATCCTACGGTATTTTATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAgg                                                                         <  1:1112549/140‑1 (MQ=255)                                                            tGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAgg                                                                         <  1:1561476/140‑1 (MQ=255)                                                             gTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGa                                                                        >  1:1978832/1‑140 (MQ=255)                                                               tATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAt                                                                      >  1:1951790/1‑140 (MQ=255)                                                               tATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAt                                                                      <  2:191358/140‑1 (MQ=255)                                                                aTGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAAtt                                                                     >  2:1621671/1‑139 (MQ=255)                                                                   tCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCa                                                                  >  2:2408086/1‑140 (MQ=255)                                                                     ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  1:2138155/140‑1 (MQ=255)                                                                     ttATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  2:2086020/140‑1 (MQ=255)                                                                       aTCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATgg                                                              >  1:2263042/1‑140 (MQ=255)                                                                        tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                             >  2:2270982/1‑140 (MQ=255)                                                                        tCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGa                                                             >  2:2038984/1‑140 (MQ=255)                                                                          ccACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATg                                                           <  2:516492/140‑1 (MQ=255)                                                                           cACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGt                                                          <  1:1721000/140‑1 (MQ=255)                                                                           acaggTATTTAATATTGCTCATCAGGATGTTCATTTCTTCATTTTGCACATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGt                                                          <  2:703048/137‑1 (MQ=255)                                                                             cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGTTGGCTTATATAACATAGAATTTAAATCAAGGAATCTCAATGGATGTTa                                                        >  2:721279/1‑140 (MQ=255)                                                                             cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                >  2:1567252/1‑132 (MQ=255)                                                                             cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAAt                                                                <  1:1567252/132‑1 (MQ=255)                                                                             cGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTa                                                        >  2:539140/1‑140 (MQ=255)                                                                               gTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTaaa                                                      <  2:2295274/140‑1 (MQ=255)                                                                               gTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTaaa                                                      <  2:1951790/140‑1 (MQ=255)                                                                               gTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTaaa                                                      <  2:2435447/140‑1 (MQ=255)                                                                                 aTTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACa                                                    <  2:73296/140‑1 (MQ=255)                                                                                  tttAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACaa                                                   >  1:265710/1‑140 (MQ=255)                                                                                  tttAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACaa                                                   >  1:1408553/1‑140 (MQ=255)                                                                                          tgTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAGAGCAAGGAATCTCAGTGGATGGTAAACAAAATgctgt                                           >  1:703048/2‑136 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTATAGCAAGGTATCACATTGGATGTTAAACAAAATGAGAt                                           >  1:1109753/1‑140 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATga                                              <  2:2393609/137‑1 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATga                                              >  1:2393609/1‑137 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  1:2170240/1‑140 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAt                                           >  1:414043/1‑140 (MQ=255)                                                                                          ggTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTGAAAACAAGGAATCTCAGTGGACGTTAAACAAAATGAGAt                                           >  2:892234/1‑140 (MQ=255)                                                                                           gTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAAtctcatt                                          <  1:2408086/140‑7 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  2:1484559/1‑140 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  2:262006/1‑140 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  2:1732217/1‑140 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  1:2075298/1‑140 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGAttt                                         >  2:219214/1‑140 (MQ=255)                                                                                            ttCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAAGAAAATTTAGAGCGAGGAATCTCAATGGATGGTAAACAAAATGAGAttt                                         >  2:693137/1‑140 (MQ=255)                                                                                                 tAGGATGTTTATTTCTTGATTTGGCATATGACTATCTTA‑TCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGa                                    <  2:871077/140‑1 (MQ=255)                                                                                                 tAGGATGTTTATTTCCTGATTTTGCCTATAAGTATATT‑‑TCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaa                                   <  1:839773/140‑1 (MQ=255)                                                                                                  aGGATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGaa                                   <  2:1978920/140‑1 (MQ=255)                                                                                                    gATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAg                                 <  2:265710/140‑1 (MQ=255)                                                                                                    gATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAg                                 >  1:1690803/1‑140 (MQ=255)                                                                                                    gATGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAg                                 <  2:1408553/140‑1 (MQ=255)                                                                                                     aTGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGc                                >  1:236672/1‑140 (MQ=255)                                                                                                      tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               >  2:2103428/1‑140 (MQ=255)                                                                                                      tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               >  1:1935665/1‑140 (MQ=255)                                                                                                      tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               >  1:2417869/1‑140 (MQ=255)                                                                                                      tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               <  1:219214/140‑1 (MQ=255)                                                                                                      tGTTTATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCa                               <  1:2471817/140‑1 (MQ=255)                                                                                                        tttATTTCTTGATTTTTGATATCAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAAt                             <  2:1109753/140‑1 (MQ=255)                                                                                                          tATTTCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATaa                           >  1:1528020/1‑140 (MQ=255)                                                                                                              tCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAatta                       >  1:1242483/1‑140 (MQ=255)                                                                                                              tCTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAatta                       <  2:1393003/140‑1 (MQ=255)                                                                                                               cTTGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattat                      >  2:1629118/1‑140 (MQ=255)                                                                                                                ttGATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAattatt                     >  1:229564/1‑140 (MQ=255)                                                                                                                  gATTTTGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGa                   >  1:306839/1‑140 (MQ=255)                                                                                                                    ttttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACt                 <  1:1454618/140‑1 (MQ=255)                                                                                                                    ttttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACt                 <  2:2170240/140‑1 (MQ=255)                                                                                                                     tttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACtt                <  1:1465376/140‑1 (MQ=255)                                                                                                                     tttGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACtt                <  1:1710693/140‑1 (MQ=255)                                                                                                                       tGCATATGAGTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCg              >  1:3025/1‑140 (MQ=255)                                                                                                                                gTATATTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGAtt     <  1:2270982/140‑1 (MQ=255)                                                                                                                                  atatTA‑CCCCCCCTCTAAAAAACAAGTTACTTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTg   <  1:721279/140‑1 (MQ=255)                                                                                                                                  atatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTg   <  1:539140/140‑1 (MQ=255)                                                                                                                                   tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTTAAAACAATAAATAATTGACTTCGTTTTAGATTTGt  >  2:981557/1‑140 (MQ=255)                                                                                                                                   tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  >  2:2107253/1‑140 (MQ=255)                                                                                                                                   tatTA‑CCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGt  >  2:1871606/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CTGAAAAAGATATACCACATGTAGGTTGAATTACCGTGTCAATTACTATCCACTTCATTTGTTATGTCTTATCCCACGGTATTTAATATGGTTCATTAGGATGTTTATTTCTTGATTTTGCATATGAGTATATTACCCCCCCCTCAAAAAAATAAATTAATTAAAATGATGGCTTATATAAAATAAAATTTAAAGCAAGGAATCTCAATGGATGTTAAACAAAATGAGATTTTGTGAAAGCAATAAATTATTGACTTCGTTTTAGATTTGT  >  NC_000913/1211946‑1212216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑