BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,426,785	(T)_6→7	coding (4/783 nt)	hisJ ←	histidine ABC transporter periplasmic binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,426,779	1	.	T	95.5%	522.3 / 12.1	157	coding (10/783 nt)	hisJ	histidine ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand): ref base . (3/4); new base T (47/103); total (50/107)
Fisher's exact test for biased strand distribution p-value = 6.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 184 total aligned reads displayed.

CAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑T‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAA  >  NC_000913/2426641‑2426912

cAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t                                                                                                                                           >  1:1537184/1‑140 (MQ=255)

cAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t                                                                                                                                           <  1:1370536/140‑1 (MQ=255)

  aCCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t                                                                                                                                       <  1:1769357/140‑1 (MQ=255)

  aCCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t                                                                                                                                       >  1:2164530/1‑140 (MQ=255)

  aCCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t                                                                                                                                       >  1:1445242/1‑140 (MQ=255)

   ccAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t                                                                                                                                     >  1:233971/1‑140 (MQ=255)

          gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAATCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  1:313198/140‑6 (MQ=255)

          gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  1:560188/140‑6 (MQ=255)

          gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  2:459690/140‑6 (MQ=255)

          gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  2:1669749/140‑6 (MQ=255)

          gCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  1:1995713/140‑6 (MQ=255)

          gCCTTGTGAATTTTTCGATTCAAATAGCGGATAGGTCAGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑t‑t‑t‑t‑tttcata                                                                                                                             <  1:995273/140‑6 (MQ=255)

           ccTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATaa                                                                                                                            >  2:386631/1‑140 (MQ=255)

                     tttttGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAAc                                                                                                                  <  2:1537184/140‑1 (MQ=255)

                       tttGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTg                                                                                                                <  1:490399/140‑1 (MQ=255)

                         tGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGAc                                                                                                              <  1:1340418/140‑1 (MQ=255)

                         tGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGAc                                                                                                              <  2:233971/140‑1 (MQ=255)

                          gATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCt                                                                                                                      >  1:447716/1‑131 (MQ=255)

                           aTTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAg                                                                                                            <  2:330829/140‑1 (MQ=255)

                            ttCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑T‑T‑TAT‑TT‑CATAATATCCTCAACTGACAGt                                                                                                           >  1:841909/1‑140 (MQ=255)

                              cAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCt                                                                                                         <  2:1351243/140‑1 (MQ=255)

                              cAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCt                                                                                                         <  2:338470/140‑1 (MQ=255)

                               aaaTGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTc                                                                                                          >  2:2434548/1‑138 (MQ=255)

                               aaaTGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTc                                                                                                          <  1:2434548/138‑1 (MQ=255)

                                aaTGGCGCATAGGTCGGGTCGGTACCGATGCGGCTGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCttt                                                                                                       <  1:1896705/140‑1 (MQ=255)

                                    gcgcATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTtat                                                                                                   <  1:2406223/140‑1 (MQ=255)

                                         tAGGTCGGGTCGGTGACGATGCTGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                                                                              <  1:862316/140‑1 (MQ=255)

                                         tAGGTCGGGTCGGTATCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                                                                              <  1:853118/140‑1 (MQ=255)

                                         tAGGTCGGGTCGGTATCGATGCCGACGTTGTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                                                                              <  1:775999/140‑1 (MQ=255)

                                         tAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                                                                              <  2:1231134/140‑1 (MQ=255)

                                         tAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGa                                                                                              <  1:2051470/140‑1 (MQ=255)

                                               gggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTGAACTGATAGTCTTGTTATAGGGAATAttt                                                                                        >  1:1166961/1‑140 (MQ=255)

                                               gggtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATAttt                                                                                        >  1:1532959/1‑140 (MQ=255)

                                                 gtcggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAAGGTCCTCATCTGCCAATCTTTTTATAGGGAATATTTAc                                                                                      >  1:1122740/1‑140 (MQ=255)

                                                   cggTACCGATGCGGATGTTTTGTGGCATCACAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  1:940917/140‑1 (MQ=255)

                                                   cggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATGTTTACAg                                                                                    <  2:1462295/140‑1 (MQ=255)

                                                   cggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  1:214120/140‑1 (MQ=255)

                                                   cggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  1:1755851/140‑1 (MQ=255)

                                                   cggTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  1:1232267/140‑1 (MQ=255)

                                                   cggTACCGATGCGGACGTTTTGAGGCATCTCAGCAAATGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  1:1147134/140‑1 (MQ=255)

                                                   aggTACCGATGCGGATGCTTTGTGGAATCGCTGCAAACGCCGCAGTTGAGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    <  2:698742/139‑1 (MQ=255)

                                                     gTACCGATGCGGATGTTTTGCGTAATCGCAGAAAACGCCCCAGTTGCGGTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                  <  2:842221/140‑1 (MQ=255)

                                                     gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                  <  2:1893160/140‑1 (MQ=255)

                                                     gTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                  >  1:2421573/1‑140 (MQ=255)

                                                      tACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑T‑T‑T‑T‑TTGCATAATGTTCTCGACTGACAGTCTTATTATAGGGAATATTTACAGGtt                                                                                 >  2:692181/1‑140 (MQ=255)

                                                         cGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                                                                              <  2:1819392/140‑1 (MQ=255)

                                                         cGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGt                                                                              <  1:220897/140‑1 (MQ=255)

                                                           aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCATCTGACAGGCTTTTTATCGGGAATATTTACAGGTTTGTTa                                                                            >  2:940917/1‑140 (MQ=255)

                                                           aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAGCTGTCAGTCTTTTTATGGGGAATATTTACAGGTTTGTTa                                                                            >  2:645425/1‑140 (MQ=255)

                                                           aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGt                                                                                  >  1:1875794/1‑134 (MQ=255)

                                                           aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCCTTTAATAGGGAATATTTACAGGTTTGTTa                                                                            >  1:1075064/1‑140 (MQ=255)

                                                           aTGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACGGACAGTCCTTTGATAGGGAATGTTTACAGGTTTGTTa                                                                            >  1:1030669/1‑140 (MQ=255)

                                                                gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTa                                                                                       >  1:1955229/1‑124 (MQ=255)

                                                                gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTa                                                                                       <  2:1955229/124‑1 (MQ=255)

                                                                gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAg                                                                                    >  1:360310/1‑127 (MQ=255)

                                                                gATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACtt                                                                       >  2:606468/1‑140 (MQ=255)

                                                                  tGTTTTGCAGAATCGCGTCAAAAGCCGCAGACGCGCTGGGGAAGGCCATAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTAt                                                                     <  2:1179894/140‑1 (MQ=255)

                                                                   gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATc                                                                    <  1:1503321/140‑1 (MQ=255)

                                                                   gTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATc                                                                    <  2:1532959/140‑1 (MQ=255)

                                                                    ttttGCGGGATCGCAGCCAACGACGCAGTTGAGCTCGAGCAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg                                                                   <  2:1166961/140‑1 (MQ=255)

                                                                    ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg                                                                   <  2:524021/140‑1 (MQ=255)

                                                                    ttttGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCg                                                                   <  1:541244/140‑1 (MQ=255)

                                                                         cGGAATCGTAGCAAACACCGCAGTTGCGCTCTAAACGGCCAGAACCAGTGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa                                                              <  2:1143596/140‑1 (MQ=255)

                                                                         cGGAATCGCAGCAAACGACGCAGTTGCGCTGGATACGGCGAGAACCAGCGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCa                                                              <  2:1075064/140‑1 (MQ=255)

                                                                              tCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATaaaa                                                         >  1:2299522/1‑140 (MQ=255)

                                                                                       aCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATc                                                <  2:1445242/140‑1 (MQ=255)

                                                                                       aCGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTCTGTTACACTTATCGTGCCATAAAATTGACCATc                                                <  2:2164530/140‑1 (MQ=255)

                                                                                          ccgcAGTTGCGCTGGAGCAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGACCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAg                                             >  1:278485/1‑140 (MQ=255)

                                                                                          ccgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAg                                             <  2:2164157/140‑1 (MQ=255)

                                                                                           cgcAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCAt                                                 >  2:262722/1‑135 (MQ=255)

                                                                                               gTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCaaa                                        <  2:1305571/140‑1 (MQ=255)

                                                                                                   cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAgg                                            >  1:177992/1‑132 (MQ=255)

                                                                                                   cgcTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAatat                                    >  2:1987444/1‑140 (MQ=255)

                                                                                                      tGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATccc                                 <  2:238693/140‑1 (MQ=255)

                                                                                                       ggAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCg                                <  2:1580492/140‑1 (MQ=255)

                                                                                                        gagaAGGCCAGAACCAGAGAGAACGATAGCAGCAG‑T‑T‑T‑TATT‑CATAATATCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGc                               <  2:747429/140‑1 (MQ=255)

                                                                                                           aaGGCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGcccc                            >  1:351234/1‑140 (MQ=255)

                                                                                                             ggCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGt                          <  2:562541/140‑1 (MQ=255)

                                                                                                              gCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGtt                         <  1:2142502/140‑1 (MQ=255)

                                                                                                              gCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGtt                         <  2:613743/140‑1 (MQ=255)

                                                                                                              gCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGtt                         <  2:5064/140‑1 (MQ=255)

                                                                                                              gCCAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGtt                         <  1:1591499/140‑1 (MQ=255)

                                                                                                                cAGAACCAGAGAGAGCGATAGCTCCTG‑T‑TCT‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                       <  1:645425/140‑1 (MQ=255)

                                                                                                                cAGAACCAGAGAGAGCGATAGCCCCAG‑T‑TCT‑T‑TT‑CATCATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACTCTTATCGTGCCATAAAATTGACAATCAAGGCAAAATATCCCGCCCCGTTAg                       <  1:680591/140‑1 (MQ=255)

                                                                                                                cAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                       <  1:1661412/140‑1 (MQ=255)

                                                                                                                cAGAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                       <  2:2299522/140‑1 (MQ=255)

                                                                                                                  gAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAGATTGACCTTCATGGCAAAATAGCCCGCCCCGTTAGAc                     >  1:1053031/1‑140 (MQ=255)

                                                                                                                  gAACCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGAc                     >  2:1947354/1‑140 (MQ=255)

                                                                                                                   aaCCAGAGAGAGCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACg                    >  2:2245587/1‑140 (MQ=255)

                                                                                                                        gagagagCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                       >  1:2476631/1‑132 (MQ=255)

                                                                                                                        gagagagCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGtatta               >  2:2433317/1‑140 (MQ=255)

                                                                                                                             agCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAg                       >  1:148987/1‑127 (MQ=255)

                                                                                                                             agCGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAttttt          >  2:1320982/1‑140 (MQ=255)

                                                                                                                             agCGATAGCACCAG‑TAT‑T‑T‑TT‑CCTAATGTACTCAACTTACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTAttttt          <  1:694032/140‑1 (MQ=255)

                                                                                                                               cGATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGG‑TTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCAt       >  2:176118/1‑140 (MQ=255)

                                                                                                                                gATAGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGTCAAAATATCCCCCCGCGTTAGACGTATTATTTTTCAt       >  1:1177487/1‑140 (MQ=255)

                                                                                                                                   aGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaa    <  2:1844909/140‑1 (MQ=255)

                                                                                                                                   aGCACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaa    <  2:1791676/140‑1 (MQ=255)

                                                                                                                                   aGCACCAGTT‑T‑T‑T‑TT‑CAAAATGTCCTCAACTGACAATCTGTTTATAGGAAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaa    <  2:891928/140‑1 (MQ=255)

                                                                                                                                     cACCAGTT‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTaaaa  >  2:2096339/1‑140 (MQ=255)

CAACCAGTTCGCCTTGTGAATTTTTTGATTCAAATGGCGCATAGGTCGGGTCGGTACCGATGCGGATGTTTTGCGGAATCGCAGCAAACGCCGCAGTTGCGCTGGAGAAGGCCAGAACCAGAGAGAGCGATAGCACCAG‑T‑T‑T‑T‑TT‑CATAATGTCCTCAACTGACAGTCTTTTTATAGGGAATATTTACAGGTTTGTTACACTTATCGTGCCATAAAATTGACCATCAAGGCAAAATATCCCGCCCCGTTAGACGTATTATTTTTCATTAAAA  >  NC_000913/2426641‑2426912

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`