BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,602,017	(C)_7→8	coding (200/2019 nt)	hyfB →	hydrogenase 4 component B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,602,010	1	.	C	88.5%	481.0 / 50.0	157	coding (193/2019 nt)	hyfB	hydrogenase 4 component B
Reads supporting (aligned to +/- strand): ref base . (7/11); new base C (66/73); total (73/84)
Fisher's exact test for biased strand distribution p-value = 6.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACGTGCTGGGGTAACTATAGTCGCCCG‑TTTT‑g                                                                                                                                              >  1:788175/1‑140 (MQ=255)

tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑g                                                                                                                                              >  2:170650/1‑140 (MQ=255)

tCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑g                                                                                                                                              >  1:1430525/1‑140 (MQ=255)

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c                                                                                                                                         >  1:1935514/1‑140 (MQ=255)

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c                                                                                                                                         >  2:2372029/1‑140 (MQ=255)

  tGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c                                                                                                                                         <  2:1784691/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c‑c‑cc                                                                                                                                    <  2:1430525/140‑1 (MQ=255)

     cTTCGCTGTTTTTACTCCGTCTCGACAGACTGGCTCTTACGCTTTCCGCCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c‑c‑cc                                                                                                                                    <  2:722797/140‑1 (MQ=255)

     cTTCGCTGGTTTTACTCTGTCTGGACAGACTCGCTCTTAGGCTTTCCGCCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑c‑c‑c‑cc                                                                                                                                    <  2:1148797/140‑1 (MQ=255)

                  aCTCGGTCTGGACAGACTGGCTTTTAAGCTTCCCGCCATTACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAAttt                                                                                                                      <  2:862419/140‑1 (MQ=255)

                  aCTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAAttt                                                                                                                      <  1:2094230/140‑1 (MQ=255)

                   cTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTg                                                                                                                     >  2:1420102/1‑140 (MQ=255)

                    tCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGc                                                                                                                    <  1:121054/140‑1 (MQ=255)

                          tGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGAttt                                                                                                              <  2:1385591/140‑1 (MQ=255)

                                 aCTGGCTGTTAAGCTTTTCGGCCTCACATCGGTCGTGGGCGGCGTGATCGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       <  2:1079550/140‑1 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  1:424724/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  2:1987097/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  1:2418908/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  2:556278/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  2:1459529/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  1:1289171/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  1:1659606/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTg                                                                                                       >  2:1774653/1‑140 (MQ=255)

                                 aCTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑GTTT‑G‑‑C‑C‑C‑TCCC‑TTTATGAATTAGCCGATTTAACCCTg                                                                                                       >  1:799516/1‑140 (MQ=255)

                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGc                                                                                                      >  1:184089/1‑140 (MQ=255)

                                  cTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTGAGTCGCCCG‑TTTG‑G‑‑C‑CAC‑CCCC‑TTT‑CGAATTTGCCGATTTAACCCTGc                                                                                                      >  2:795324/1‑140 (MQ=255)

                                   tGGCTATTAAGCTTTCCAGCATCACATCGCTCGTGCGCGCCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCg                                                                                                     <  2:810917/140‑1 (MQ=255)

                                    ggCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGa                                                                                                    <  1:64820/140‑1 (MQ=255)

                                     gCTATTAATCTTTCCGGTATCACATCGCTGGTGGGGGGCTTGATTGGCCTCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGaa                                                                                                   <  2:1197072/140‑1 (MQ=255)

                                     gCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTTCG‑‑C‑C‑C‑CCCC‑TTT‑TTAATTTGCCGATTTAACCCTGCGaa                                                                                                   >  2:271395/1‑140 (MQ=255)

                                           aaGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCT‑TTTT‑G‑‑C‑C‑CGCCCC‑TTT‑TGAATTTGCAGATTGAACCCTGCGAATGGATa                                                                                             >  2:745386/1‑140 (MQ=255)

                                            aGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAg                                                                                            <  1:1819338/140‑1 (MQ=255)

                                               tttCCGGCATCACATCGCTGGTGGACGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                         <  1:1435115/140‑1 (MQ=255)

                                               tttCCGGCATCACATCGCTGGTAGGCGGCGTGATTAGCACCATCAGCGCAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCct                                                                                         <  2:1101395/140‑1 (MQ=255)

                                                ttCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCctc                                                                                        <  1:25424/140‑1 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGAt                                                                                              <  2:614290/133‑1 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       >  2:1838830/1‑140 (MQ=255)

                                                 tCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCctct                                                                                       >  1:2187729/1‑140 (MQ=255)

                                                   cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCg                                                                                     >  2:8787/1‑140 (MQ=255)

                                                   cGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTGAATCCTGCGTATGGATAGCCTCTCg                                                                                     >  1:1150989/1‑140 (MQ=255)

                                                         cacaTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCAttt                                                                               <  1:2321245/140‑1 (MQ=255)

                                                          acaTCGCTGGTGGGCGGTGTGATTGGCATCATCAGCGGACTTACGCAAATACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTa                                                                              <  2:1010777/140‑1 (MQ=255)

                                                           caTCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑GTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTAt                                                                             >  1:441790/1‑140 (MQ=255)

                                                               gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         <  1:1875340/140‑1 (MQ=255)

                                                               gCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTg                                                                         <  1:1691696/140‑1 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     >  1:1870970/1‑140 (MQ=255)

                                                                   gtggGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑GTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTgg                                                                     <  2:441790/140‑1 (MQ=255)

                                                                      ggcggcGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTa                                                                  >  1:1914997/1‑140 (MQ=255)

                                                                      ggcggcGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCATGCCAATAGATAGCCTATCGGAATTTATGGTGCTGGTTa                                                                  >  2:990034/1‑140 (MQ=255)

                                                                        cggcgTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTAtc                                                                >  1:586693/1‑140 (MQ=255)

                                                                               aTTGGCATCATCAGCTGAATTACGCCATTTCATGCTGATGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGc                                                         <  1:702253/140‑1 (MQ=255)

                                                                                    catcatCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    >  1:2254260/1‑140 (MQ=255)

                                                                                    catcatCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtg                                                    <  1:149857/140‑1 (MQ=255)

                                                                                     atcatcAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTGTCGGCATATATCGTGCTGGTTATCTCCTTGCtggtgg                                                   >  1:1080811/1‑140 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                                <  2:1914997/140‑1 (MQ=255)

                                                                                        atcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCtggtggtgg                                                <  1:488752/140‑1 (MQ=255)

                                                                                         tcaGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTGTGGTGCTGTTTATCTCCTTGCTggtggtggt                                               >  2:934581/1‑140 (MQ=255)

                                                                                             cgaaaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:2372029/137‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  1:556278/140‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:1220414/140‑1 (MQ=255)

                                                                                             cGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:91225/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:424338/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:573815/140‑1 (MQ=255)

                                                                                              ggAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGtt                                          <  2:2474959/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  1:2146745/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  1:1459529/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  1:1622815/140‑1 (MQ=255)

                                                                                               gAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  2:1289171/140‑1 (MQ=255)

                                                                                                aaTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtct                                        <  2:2187729/140‑1 (MQ=255)

                                                                                                 aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGCTGGTGGTTTGTtctc                                       >  1:895687/1‑140 (MQ=255)

                                                                                                 aTTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGACTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctc                                       <  1:2206094/140‑1 (MQ=255)

                                                                                                   tACGCAATTACATGCTGTTGTAACTTTAGTCTCCCG‑TTTG‑G‑‑C‑C‑C‑CCCCGTTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  2:1170799/140‑1 (MQ=255)

                                                                                                   tACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  1:170650/140‑1 (MQ=255)

                                                                                                   tACGCAATTACATGCAGGTGTAGCTATAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  1:1137682/140‑1 (MQ=255)

                                                                                                     cGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GCCC‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    <  2:2116981/140‑1 (MQ=255)

                                                                                                      gCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTAt                                  <  1:2023278/140‑1 (MQ=255)

                                                                                                         aTTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCa                               <  2:2254260/140‑1 (MQ=255)

                                                                                                         aTTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  1:1379623/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAAGTTTAGTCGCCCG‑TTAT‑G‑‑CTC‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  2:1150989/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGGCCG‑ATTT‑G‑‑C‑C‑CTCCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  1:990034/140‑1 (MQ=255)

                                                                                                          ttACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCAt                              <  1:1770060/140‑1 (MQ=255)

                                                                                                             cATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtc                                         <  1:280189/125‑1 (MQ=255)

                                                                                                              aTGCTGGTGTAACTTTAGTCGCTCGCTTTT‑C‑‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGAc                          <  1:861691/140‑1 (MQ=255)

                                                                                                               tGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGt                                           <  2:2279127/122‑1 (MQ=255)

                                                                                                               tGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACt                         >  1:117130/1‑140 (MQ=255)

                                                                                                                   ggTGTAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTtata                     <  2:1659606/140‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    >  1:2381971/1‑124 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTctctct                                    <  2:2381971/124‑1 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTatat                    >  2:367149/1‑140 (MQ=255)

                                                                                                                    gtgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGGTATCTCCTAGCTGGGGGTGGTTTGTACTCTGCATTCATTTACTTatat                    >  2:1210181/1‑140 (MQ=255)

                                                                                                                     tgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     >  1:387035/1‑122 (MQ=255)

                                                                                                                     tgtAACTTTAGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTtctctc                                     <  2:387035/122‑1 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          >  2:2482050/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          <  2:1751250/140‑1 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          >  1:176491/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTATCTCTATTAATTGACTTATATGCGCGAATAc          >  2:669780/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGATTGTTCTATCTTTTGATTGACATATATGCGCGAATAc          >  1:793963/1‑140 (MQ=255)

                                                                                                                              aGTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑ATT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATAc          <  1:1980228/140‑1 (MQ=255)

                                                                                                                               gTCGCCCG‑TTTT‑GC‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACg         >  2:283877/1‑140 (MQ=255)

                                                                                                                                      g‑TTTT‑GC‑C‑C‑C‑CCCC‑TAT‑TGAATTTGGCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCaa  <  1:1203462/140‑1 (MQ=255)

TCTGGCTTCGCTGTTTTTACTCGGTCTGGACAGACTGGCTATTAAGCTTTCCGGCATCACATCGCTGGTGGGCGGCGTGATTGGCATCATCAGCGGAATTACGCAATTACATGCTGGTGTAACTTTAGTCGCCCG‑TTTT‑G‑‑C‑C‑C‑CCCC‑TTT‑TGAATTTGCCGATTTAACCCTGCGAATGGATAGCCTCTCGGCATTTATGGTGCTGGTTATCTCCTTGCTGGTGGTGGTTTGTTCTCTCTATTCATTGACTTATATGCGCGAATACGAGGGCAA  >  NC_000913/2601871‑2602143

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`