Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,734,543	(C)8→9	coding (492/732 nt)	pgeF ←	polyphenol oxidase YfiH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,734,535	1	.	C	93.3%	195.7 / 6.0	60	coding (500/732 nt)	pgeF	polyphenol oxidase YfiH
Reads supporting (aligned to +/- strand):  ref base . (2/2);  new base C (26/30);  total (28/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.69e-01

GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑CCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAT  >  NC_000913/2734396‑2734664                                                                                                                                              |                                                                                                                                    gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:39155/1‑140 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:1859168/1‑140 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:1662848/1‑140 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:1604127/1‑140 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:1239919/1‑140 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      <  1:2268041/140‑1 (MQ=255) gccgccGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCAATTGATTTCGCGTCTACTGCCATAAACGCCTCGC‑GAACCt                                                                                                                                      >  2:986359/1‑140 (MQ=255)  ccgccgAAAATTTGCTGCACACCCACGTTCACCAGCCGCGGCCGGGCAGGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑c                                                                                                                                    <  2:1185721/140‑1 (MQ=255)     ccgAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATTACCATGCTGAATAAAAGCTGTACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑cccc                                                                                                                                 <  1:1652835/140‑1 (MQ=255)       gAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑cccc‑cc                                                                                                                              <  1:377791/140‑1 (MQ=255)        aaaaTTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAG‑TGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑cccc‑ccc‑c                                                                                                                           <  1:2151756/140‑1 (MQ=255)             ttGCTCAACACCCACGTTCGCCTGATGCTCCCGGGCACGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑cccc‑ccc‑ccact                                                                                                                       <  1:1160050/140‑5 (MQ=255)                  cAACACCCACGTTCGCCAGACGCTGCCGGCCAAGCTGTTAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTTGC‑GAACCTCCCCC‑CCC‑CACTTCGaa                                                                                                                   <  1:966073/140‑1 (MQ=255)                         cACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGt                                                                                                            <  1:1327060/140‑1 (MQ=255)                         cACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGt                                                                                                            <  1:408868/140‑1 (MQ=255)                             ttCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑CCCCGCCC‑CACTTCGAACGCGCGTGGAc                                                                                                        <  1:1441040/140‑1 (MQ=255)                              tCGCCAGACGCTGCCAGGCAAGTTGTTAAATATCCGCTAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGAcc                                                                                                       <  1:910068/140‑1 (MQ=255)                                   aGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTg                                                                                                  <  1:122108/140‑1 (MQ=255)                                         tGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                                                            <  1:323292/140‑1 (MQ=255)                                         tGCCGGGCAAGCTGAAAAATATCCGCCAGTTACTTATGACCATGCTGAATGAAAGCTGCCCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGcc                                                                                            <  1:861182/140‑1 (MQ=255)                                                   gCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGc                                                                                  <  1:1300443/140‑1 (MQ=255)                                                        tAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGaa                                                                             <  1:1327766/140‑1 (MQ=255)                                                             atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                                                                       <  2:1599363/125‑1 (MQ=255)                                                             atCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAAc                                                                                       >  1:1599363/1‑125 (MQ=255)                                                              tCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATAtttt                                                                       >  2:406211/1‑140 (MQ=255)                                                                 gCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCg                                                                    >  2:1268948/1‑140 (MQ=255)                                                                      aTACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTa                                                               <  1:135055/140‑1 (MQ=255)                                                                       tACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTAt                                                              <  1:1268948/140‑1 (MQ=255)                                                                            aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                                                         <  2:1310328/140‑1 (MQ=255)                                                                            aTCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCa                                                         <  1:1604127/140‑1 (MQ=255)                                                                                 cATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACa                                                    >  2:2434464/1‑140 (MQ=255)                                                                                  aTGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAg                                                   >  1:242050/1‑140 (MQ=255)                                                                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAGCCCGCGTGGACAAATTGCCGGCCCTAACCAGCGGAGAAGTTTTTCCGGATCATCGGCaaaccgga                                                  >  2:2134270/1‑135 (MQ=255)                                                                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                  <  1:1855200/140‑1 (MQ=255)                                                                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                  >  1:2258760/1‑140 (MQ=255)                                                                                   tGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAgg                                                  >  1:1513260/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACGAGGCGAGCATACTTCCCGGATGATCAGCAAAACAGGa                                                 >  1:1040158/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGGGAATATTTTCCGGATTATCAGCAAAAAAGGa                                                 >  2:1726604/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  2:2172966/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  1:2301884/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  2:1229615/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  2:1465793/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  1:2092857/1‑140 (MQ=255)                                                                                    gCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACCCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGa                                                 >  2:1608639/1‑140 (MQ=255)                                                                                      tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa                                               <  1:2434464/140‑1 (MQ=255)                                                                                      tgaatgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGaaa                                               <  1:1239919/140‑1 (MQ=255)                                                                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt                                           >  2:2019822/1‑140 (MQ=255)                                                                                          tgaaAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGt                                           >  1:1732809/1‑140 (MQ=255)                                                                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAGCCAGGCGAGAATATTTCCCGGATTAACAGCTAAACAGGAAACCGtctc                                        >  2:746564/1‑140 (MQ=255)                                                                                             aaGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGtctc                                        >  1:1524792/1‑140 (MQ=255)                                                                                               gCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCtt                                      >  1:2109842/1‑140 (MQ=255)                                                                                                  gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCa                                   <  1:133892/140‑1 (MQ=255)                                                                                                  gCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCa                                   >  1:341480/1‑140 (MQ=255)                                                                                                    aCTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGCCCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAACCAGGAAACCGTCTCTTCCAGc                                 >  1:1551819/1‑140 (MQ=255)                                                                                                     cTTGGTTTAGCCTCTTCTGGCATTAACCCCTTGCGGACCCC‑CCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                <  1:2134270/140‑1 (MQ=255)                                                                                                     cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                <  1:1859168/140‑1 (MQ=255)                                                                                                     cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                <  1:1465793/140‑1 (MQ=255)                                                                                                     cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                <  2:2301884/140‑1 (MQ=255)                                                                                                     cTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCa                                <  1:2093884/140‑1 (MQ=255)                                                                                                             aGCGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTgcg                        >  2:443610/1‑140 (MQ=255)                                                                                                               ggTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:1601279/139‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACTCCTCCC‑GAACCTCCCCC‑CCC‑CCCTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  2:1040158/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:1662848/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:39155/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:406211/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:2172966/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  2:1513260/140‑1 (MQ=255)                                                                                                               cGTCTACTGCCATAAACGCCTCCC‑GAACCT‑CCCC‑CCCGCACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  1:986359/140‑1 (MQ=255)                                                                                                                   tACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      <  2:1874302/136‑1 (MQ=255)                                                                                                                   tACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGca                      >  1:1874302/1‑136 (MQ=255)                                                                                                                   tACTGCCATAAACGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCAAACGCGCGCGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCCAAAGAGAAAACCGTCTATTCCTGCACGCCTGCGCCCAGt                  >  2:921552/1‑140 (MQ=255)                                                                                                                              aCGCCTCGC‑GAACCTCCCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACgccagcc       <  2:1524792/140‑1 (MQ=255)                                                                                                                                  cTCGC‑GAACCT‑CCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAt  >  1:2148009/1‑140 (MQ=255)                                                                                                                                  cTCGC‑GAACCT‑CCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCAGGCCCTAACCAGGCGAGAATATTTTCCGGATTAGCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAt  >  2:226143/1‑140 (MQ=255)                                                                                                                                              |                                                                                                                                    GCCGCCGAAAATTTGCTCAACACCCACGTTCGCCAGACGCTGCCGGGCAAGCTGATAAATATCCGCCAGATACTTATCACCATGCTGAATGAAAGCTGCACTTGCTTTAGCGTCTACTGCCATAAACGCCTCGC‑GAACCT‑CCCC‑CCC‑CACTTCGAACGCGCGTGGACCAATTGCCGGCCCTAACCAGGCGAGAATATTTTCCGGATTATCAGCAAAACAGGAAACCGTCTCTTCCAGCACGCCTGCGCACAGTCCACGCCAGCCAGCAT  >  NC_000913/2734396‑2734664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑