Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,815,883	+C	coding (667/687 nt)	rph ←	truncated RNase PH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,815,879	1	.	C	96.6%	303.7 / 2.1	88	coding (671/687 nt)	rph	truncated RNase PH
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base C (40/45);  total (41/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012                                                                                                                                         |                                                                                                                                      aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑ccccct                                                                                                                                 <  2:303043/140‑3 (MQ=255) aaCTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑ccccct                                                                                                                                 <  1:1402533/140‑3 (MQ=255) aaCTGGCGCTGATATGGTTTCACGCCTTCGCTCCTGATCGTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTTG‑A‑TT‑ccccct                                                                                                                                 <  2:1002450/140‑3 (MQ=255)    tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑ccccctcgg                                                                                                                              >  2:216346/1‑135 (MQ=255)    tGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑ccccctcgg                                                                                                                              >  1:1579424/1‑135 (MQ=255)     ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGTCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCggg                                                                                                                              >  1:2275796/1‑140 (MQ=255)     ggCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCTTTCTGCGACGCTACAATGGATGCG‑A‑TTCCCCCTCggg                                                                                                                              >  1:669300/1‑140 (MQ=255)      gcgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGc                                                                                                                             >  1:225455/1‑140 (MQ=255)       cgcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGcc                                                                                                                            >  1:246766/1‑140 (MQ=255)        gcTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCa                                                                                                                           <  2:783192/140‑1 (MQ=255)           gATATGGTTTCATGCGTTCGCTCCTCATCCTACTTTTTTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCagag                                                                                                                        <  1:780693/140‑1 (MQ=255)           gATATGGTTTCATGCCTTCGCTGCTGATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCagag                                                                                                                        <  1:848132/140‑1 (MQ=255)           gATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCagag                                                                                                                        <  2:1851367/140‑1 (MQ=255)            atatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGc                                                                                                                       >  1:319138/1‑140 (MQ=255)             tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGcc                                                                                                                      <  2:246766/140‑1 (MQ=255)             tatGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGcc                                                                                                                      >  1:2469814/1‑140 (MQ=255)              atGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCca                                                                                                                     <  1:1696059/140‑1 (MQ=255)                ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGTTACAATGTATTGGAA‑TC‑CCCCGCGGGCCAGAGCcaac                                                                                                                   >  1:687728/1‑140 (MQ=255)                ggTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCcaac                                                                                                                   >  1:98924/1‑140 (MQ=255)                  tttCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCcaacaa                                                                                                                 <  2:1579424/140‑1 (MQ=255)                    tCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGa                                                                                                               >  1:1568/1‑140 (MQ=255)                      aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:2299358/1‑140 (MQ=255)                      aTGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATg                                                                                                             >  1:2250736/1‑140 (MQ=255)                       tGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGa                                                                                                            >  1:2471661/1‑140 (MQ=255)                        gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                           <  2:39791/140‑1 (MQ=255)                        gCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAg                                                                                                           <  2:89839/140‑1 (MQ=255)                         ccTTCGCTCCTCATCTTCCTTTCCTACAGACCAAACAAACGCGACTCACCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                                                                          <  2:669300/140‑1 (MQ=255)                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:2299358/140‑1 (MQ=255)                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        >  2:1256832/1‑140 (MQ=255)                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        >  1:2463592/1‑140 (MQ=255)                           ttCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:2471661/140‑1 (MQ=255)                           ttCGCTCCTCATCTTACTTTTCAACGGACAAAAAAAATGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  1:938834/140‑1 (MQ=255)                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCcaa                                                                                                                    <  2:2031330/127‑1 (MQ=255)                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCcaa                                                                                                                    >  1:2031330/1‑127 (MQ=255)                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                                                                          <  1:398723/137‑1 (MQ=255)                            tCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGt                                                                                                          >  2:398723/1‑137 (MQ=255)                             cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        >  1:337337/1‑138 (MQ=255)                             cGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAg                                                                                                        <  2:337337/138‑1 (MQ=255)                                tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCca                                                                                                                     >  2:2373165/1‑122 (MQ=255)                                tcctcATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCca                                                                                                                     <  1:2373165/122‑1 (MQ=255)                                    cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                   <  2:201125/136‑1 (MQ=255)                                    cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCtt                                                                                                   >  1:201125/1‑136 (MQ=255)                                    cATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATg                                                                                               >  2:2250382/1‑140 (MQ=255)                                         tACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGa                                                                                          <  2:467907/140‑1 (MQ=255)                                            ttttCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACg                                                                                       >  1:225311/1‑140 (MQ=255)                                               tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                           >  2:2279198/1‑133 (MQ=255)                                               tCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTg                                                                                           <  1:2279198/133‑1 (MQ=255)                                                 tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  2:363747/1‑140 (MQ=255)                                                 tACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCg                                                                                  >  1:1516034/1‑140 (MQ=255)                                                  aCAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGc                                                                                 >  2:2249383/1‑140 (MQ=255)                                                     gACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGATCGTCATGGCTGAACGGCTCGCCtt                                                                              >  1:1139817/1‑140 (MQ=255)                                                     gACAAAAAAAAGGCGAACCATCAGTCGCCTTAAAATTCACTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCtt                                                                              <  2:1139817/140‑1 (MQ=255)                                                        aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                >  1:1499432/1‑135 (MQ=255)                                                        aaaaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGcc                                                                                <  2:1499432/135‑1 (MQ=255)                                                          aaaaaaGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGcc                                                                         <  2:225455/140‑1 (MQ=255)                                                               aGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTccc                                                                    <  2:1568/140‑1 (MQ=255)                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   <  1:1256832/140‑1 (MQ=255)                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   >  2:2266574/1‑140 (MQ=255)                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTcccc                                                                   <  2:2469814/140‑1 (MQ=255)                                                                ggCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGGAGCCCTTTATGGGTGAGCGGCTCGCCTTCTGCCGTcccc                                                                   >  2:869046/1‑140 (MQ=255)                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:225311/140‑1 (MQ=255)                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:98924/140‑1 (MQ=255)                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:1793659/140‑1 (MQ=255)                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:1266610/140‑1 (MQ=255)                                                                  cGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  1:2266574/140‑1 (MQ=255)                                                                  cGACTCATCAGTCGCCTCAAAATTCAGTTTGCCAGAGCCCCCTTCTGCCTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTg                                                                 <  2:645966/140‑1 (MQ=255)                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  2:319138/140‑1 (MQ=255)                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               >  2:2205585/1‑140 (MQ=255)                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCa                                                               <  1:2250382/140‑1 (MQ=255)                                                                    aCTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCGTCATGGGGGAAGGGATCGCCTGCTGCCGTCCCCTGCa                                                               >  1:645966/1‑140 (MQ=255)                                                                        atcaGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTc                                                           <  1:2249383/140‑1 (MQ=255)                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGACCGGCTCGACTTATGGCGTCCCATGCACTTCAatgatg                                                    >  1:741120/1‑140 (MQ=255)                                                                               gCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAatgatg                                                    >  2:1316979/1‑140 (MQ=255)                                                                                 cTTAAAAATCAGTTTGTCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcg                                                  <  2:2275796/140‑1 (MQ=255)                                                                                  ttAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATgcgc                                                 >  1:1334212/1‑140 (MQ=255)                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        <  2:1712079/140‑1 (MQ=255)                                                                                           aGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCg                                        >  2:2193489/1‑140 (MQ=255)                                                                                            gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  1:1913504/140‑1 (MQ=255)                                                                                            gTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCgg                                       <  1:1593740/140‑1 (MQ=255)                                                                                             tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:1316979/140‑1 (MQ=255)                                                                                             tttGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGt                                      <  1:216346/140‑1 (MQ=255)                                                                                                gCCAGCGCCGCCTTCTGCGTCGTTATAATTGATTCGAA‑TC‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  1:1011560/140‑1 (MQ=255)                                                                                                gCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  1:2205585/140‑1 (MQ=255)                                                                                                gCCAGCGCCGCCTTCCGCGTCGCTATAATCGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcat                                   <  1:869046/140‑1 (MQ=255)                                                                                                  cAGCGCCGCCTTCTGCGTCGCTCCAATGGATTCG‑ACTC‑CCCCTCGGGCTAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGtcatca                                 <  2:741120/140‑1 (MQ=255)                                                                                                     cgccgcCTTCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTa                              >  1:2250139/1‑140 (MQ=255)                                                                                                            ttCTGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTAGGTCATCACTACGTTCAt                       >  2:2117569/1‑140 (MQ=255)                                                                                                               tGCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTc                    >  2:2248047/1‑140 (MQ=255)                                                                                                                gCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  2:2340630/140‑1 (MQ=255)                                                                                                                gCGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCg                   <  2:2250736/140‑1 (MQ=255)                                                                                                                 cGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCGGTCTTCGGTCATCACTACGTTCATGTCgg                  >  1:1013351/1‑140 (MQ=255)                                                                                                                 cGTCGCTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCgg                  >  1:266444/1‑140 (MQ=255)                                                                                                                      cTACAATGGATTCG‑A‑TTCCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCACCCGTCTGCGGACAGCACTACATTCATGTCGGTctct             >  1:712994/1‑140 (MQ=255)                                                                                                                              gattcg‑a‑ttcCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTAGGTCATCACTACGTTCATGTCGGTCTCTGCGGCaga     <  1:2117569/140‑1 (MQ=255)                                                                                                                                 tcg‑a‑ttcCCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTc  >  2:1296753/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      AACTGGCGCTGATATGGTTTCATGCCTTCGCTCCTCATCTTACTTTTCTACAGACAAAAAAAAGGCGACTCATCAGTCGCCTTAAAAATCAGTTTGCCAGCGCCGCCTTCTGCGTCGCTACAATGGATTCG‑A‑TT‑CCCCTCGGGCCAGAGCCAACAAGATGAGTAGCTCTTCATGGGTGAACGGCTCGCCTTCTGCCGTCCCCTGCACTTCAATGATGCGCCCGTCTTCGGTCATCACTACGTTCATGTCGGTCTCTGCGGCAGAGTC  >  NC_000913/3815746‑3816012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑