BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,753	(T)_5→6	coding (339/450 nt)	yidI →	putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,748	1	.	T	90.6%	377.3 / 28.5	129	coding (334/450 nt)	yidI	putative inner membrane protein
Reads supporting (aligned to +/- strand): ref base . (7/5); new base T (47/69); total (54/75)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

CGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑T‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGC  >  NC_000913/3856612‑3856880

 gTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCTCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑t‑t‑tt                                                                                                                                     >  1:544635/1‑140 (MQ=255)

  tGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑t‑t‑ttt                                                                                                                                    >  1:2481602/1‑140 (MQ=255)

  tGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑g‑t‑ttt                                                                                                                                    >  2:396019/1‑140 (MQ=255)

         aaaaGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑ggcggc                                                                                                                             <  1:1292458/140‑1 (MQ=255)

               ggACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACg                                                                                                                       <  1:1366076/140‑1 (MQ=255)

               ggACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGAGAAATCGCAGGGACCTACGCGGCGC‑GCT‑G‑GAT‑T‑TTT‑GGCGGCgggcac                                                                                                                       >  2:1101862/1‑137 (MQ=255)

                gACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTTGGGGATTAGCGGCGC‑GGT‑G‑G‑TGT‑TTT‑GGCGGCGGGgcgc                                                                                                                      >  2:699227/1‑140 (MQ=255)

                  cGTAGATGTCGATAAAATCCTCAATCAAGGCGGTATCCCACGGGCCATTGCTCGGCTCCTTGGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑T‑T‑TTT‑GGCGGCGGGACGCTgg                                                                                                                   <  2:490487/140‑1 (MQ=255)

                  cGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTg                                                                                                                    <  2:1248657/140‑1 (MQ=255)

                   gTAGATGTCGATAAACTCCTCACTCCAAGCGGTATCGGACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTgg                                                                                                                   <  1:699227/140‑1 (MQ=255)

                   gTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTgg                                                                                                                   <  1:1245636/140‑1 (MQ=255)

                    tAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGc                                                                                                                  <  2:2481602/140‑1 (MQ=255)

                    tAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGc                                                                                                                  <  2:1807385/140‑1 (MQ=255)

                        tGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTGCTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTc                                                                                                              <  2:2180814/140‑1 (MQ=255)

                          tCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGGGTTccc                                                                                                            >  2:1763689/1‑139 (MQ=255)

                          tCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCttt                                                                                                               >  2:1836659/1‑137 (MQ=255)

                          tCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCa                                                                                                            >  1:105140/1‑140 (MQ=255)

                          tCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCCCGGCGC‑CCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTCCa                                                                                                            >  1:157210/1‑140 (MQ=255)

                             aTAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGGATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑TGCGGCGGGGCGCTTGCTTTTCCTAc                                                                                                         >  2:1467733/1‑140 (MQ=255)

                                        aatcaaAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTCCATACCTTG‑Ctttt‑tt                                                                                            >  2:2411969/1‑139 (MQ=255)

                                        aatcaaAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑Tg                                                                                            >  1:1694892/1‑140 (MQ=255)

                                        aatcaaAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACACTGGCTTTCGATACCTTG‑Ctttt‑tt                                                                                            >  1:679000/1‑139 (MQ=255)

                                         atcaaAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑Tgg                                                                                           <  2:1428567/140‑1 (MQ=255)

                                             aaGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑GGGGGCGGGAGGCTGGCTTGCCATACCTTG‑CTTTT‑TTGTATc                                                                                       >  2:979562/1‑140 (MQ=255)

                                              aGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATTCGTAAGGAAAATAGCCGGGGCATTCGCGGGGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTTGCTTTTCATACCCTG‑CTTTT‑TGGTAttg                                                                                      >  2:212164/1‑138 (MQ=255)

                                              aGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGGATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTCCATACCTTG‑CTTTT‑TTGttttg                                                                                      >  2:237888/1‑136 (MQ=255)

                                              aGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCg                                                                                      <  1:345675/140‑1 (MQ=255)

                                              aGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCg                                                                                      <  1:1798842/140‑1 (MQ=255)

                                                cGGTATCGCACTGGCAATTGCTCCGCTACGTTGCGCACTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGc                                                                                    <  1:952272/140‑1 (MQ=255)

                                                  gTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTCCATACCTTG‑CTTTT‑TTGGATCGGCAt                                                                                  >  2:2375087/1‑140 (MQ=255)

                                                   tATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATc                                                                                 <  2:174636/140‑1 (MQ=255)

                                                    aTCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCCGGCTTTCCCTACCTTG‑CTTTT‑TGGTTTTCGCATcc                                                                                >  2:1778176/1‑139 (MQ=255)

                                                      cGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTTCATACCTTG‑CTTTT‑TGGTATCGGCCTCgtg                                                                              >  2:1264855/1‑140 (MQ=255)

                                                      cGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCgtg                                                                              <  2:2033108/140‑1 (MQ=255)

                                                             gCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTgcagca                                                                       >  1:155923/1‑140 (MQ=255)

                                                             gCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTgcagca                                                                       >  2:2082447/1‑140 (MQ=255)

                                                             gCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTgcagca                                                                       >  1:1758205/1‑140 (MQ=255)

                                                             gCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTgcagca                                                                       >  1:1762685/1‑140 (MQ=255)

                                                             gCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGCAAGGAAAATCGCTGGGGCATTTGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTCTCCGTACGCTG‑CTTTTATGGTGT‑GGCCTCCTGTgcagca                                                                       >  2:677823/1‑140 (MQ=255)

                                                                aTTGCTGCGCTACTTAGCGCATGTATTGGCGGTATGCTTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCAttt                                                                    <  1:1101862/140‑1 (MQ=255)

                                                                 ttGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCAtttt                                                                   <  1:344570/140‑1 (MQ=255)

                                                                 ttGCTGCGCTACTTTACGCATTTATAGGCGGTATGCGAAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCAtttt                                                                   <  1:1135993/140‑1 (MQ=255)

                                                                  tgctgcGCTACTTTGCGGATTTTTTGGCGGTTTGCGTAAAGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTg                                                                  <  2:865767/140‑1 (MQ=255)

                                                                  tgctgcGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGGAAATCGCTGGGGCATTCGCGGCGCGGCT‑G‑G‑T‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTTCATACCTTG‑CCTTT‑TGGGATTGGCCTCGCGTGGAGCATTTTg                                                                  >  2:344570/1‑140 (MQ=255)

                                                                  tgctgcGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAAACGCTGGGGCATTCGCGGCGC‑GCC‑G‑GTT‑T‑TTT‑TGCGGCGGGGCGCCAGCTTTCCATACCTTGCCTTTT‑TGGTA‑CGGCAACGTGTGCAGCAttttt                                                                  >  2:821379/1‑139 (MQ=255)

                                                                     tgcGCTACTTTGCGCATGTATTCGCGGTATTTGTACGGATAATCGCAGTGGCATTCGCGGCGC‑GCT‑G‑G‑T‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGa                                                              <  2:1199590/140‑1 (MQ=255)

                                                                     tgcGCTACGTTGCGCACTTATTTGCGGTATGTGAACGGACAATCGCTGTGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTg                                                               <  2:1104194/140‑1 (MQ=255)

                                                                      gcgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGa                                                              <  1:1264855/140‑1 (MQ=255)

                                                                      gcgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGa                                                              <  2:2036817/140‑1 (MQ=255)

                                                                      gcgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGa                                                              <  1:237888/140‑1 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGTGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGat                                                             <  1:979562/140‑1 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGCGGTATTGGTAAGGAAAATCCCTGGGGCATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑GGCGGGGGGGCGCTGGCTTTTCCTACCCTT‑CTTTT‑TTGTATTGGCATCGGGTTCAGCATTTTTCTggt                                                             >  2:612505/1‑138 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGat                                                             >  1:585080/1‑140 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGat                                                             >  1:1854330/1‑140 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGat                                                             >  2:3910/1‑140 (MQ=255)

                                                                       cgcTACTTTGCGCATTTATTGGCGGTATGCGGAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGGTTTTCATACCTTG‑CTTTT‑TTGTATCGGGATCGGGTGCAGCATTTTTCTGat                                                             >  2:1638971/1‑140 (MQ=255)

                                                                       agacagtttGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCAGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGat                                                             <  1:627972/134‑1 (MQ=255)

                                                                        gcTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGata                                                            <  2:1670309/140‑1 (MQ=255)

                                                                        gcTACTTTGCGCATTAATTGGCAGTATGCGTAAGGAATATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGata                                                            <  1:1102350/140‑1 (MQ=255)

                                                                         cTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATTGGCATCGTGTGCAGCATTTTGCTGgtat                                                           >  2:1224070/1‑140 (MQ=255)

                                                                           aCTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTc                                                         <  2:1802652/140‑1 (MQ=255)

                                                                            cTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTcc                                                        >  1:2233777/1‑140 (MQ=255)

                                                                            cTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTcc                                                        <  1:2136456/140‑1 (MQ=255)

                                                                            cTTTGCGCAATAACTGGCGATATGCGTAAGGACAATCGCTGGGGCATTCCCCGACC‑CCT‑T‑G‑T‑T‑TTTCGGCGGCGGGATGCTGGCTTTCCATAACTTG‑CTTTA‑TGGTATCGGTATCGTGCGCAGCATTTTGCTGATATTcc                                                        <  2:1526758/140‑1 (MQ=255)

                                                                               tGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTg                                                                  >  1:1372601/1‑127 (MQ=255)

                                                                               tGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTg                                                                  <  2:1372601/127‑1 (MQ=255)

                                                                                      ttATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGGCGCTGGGTTTTCATACCTTG‑CTTTT‑TTGTATCGGCATCGTGTGCAGCATTTTGGTGATATTCCTTATCttttt                                              >  2:2423177/1‑139 (MQ=255)

                                                                                        aTTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCTGG‑G‑T‑T‑TTT‑GGGGGCGGGGCGCTGGCTTTCCATACCTTG‑CTTTT‑TTGTATCGGCGTCGTGTGAAGCATTTTGCTGATATTCCttttctttttct                                            >  2:1006901/1‑140 (MQ=255)

                                                                                               gTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGAc                                     <  1:2082447/140‑1 (MQ=255)

                                                                                                tATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TTGTATCGGCATCGTGTGTAGCGTTTCTCTGGTATGTCTTATCTTTTCCTTTCTGAcc                                    >  2:639386/1‑140 (MQ=255)

                                                                                                tATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGAcc                                    >  2:133252/1‑140 (MQ=255)

                                                                                                      tAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGt                              >  2:457457/1‑140 (MQ=255)

                                                                                                      tAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTGGCTGATATTCCATAGCTTTTCGTTCCTGACCGGCGGt                              >  1:781425/1‑140 (MQ=255)

                                                                                                       aaGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGtt                             >  1:1642923/1‑140 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  2:609727/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  1:2469828/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  2:2233777/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  2:1854330/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  2:1758205/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  1:483726/140‑1 (MQ=255)

                                                                                                         ggAAAATCGCTGGGGCACTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGAGGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCa                           <  2:780185/140‑1 (MQ=255)

                                                                                                          gAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTAATATTCCGTATGTTTTCCTTCATGACCGGCGGTTCAc                          >  1:1041690/1‑140 (MQ=255)

                                                                                                           aaaaTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACt                         >  2:1377554/1‑140 (MQ=255)

                                                                                                           aaaaTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACt                         <  2:157210/140‑1 (MQ=255)

                                                                                                            aaaTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATAGTCCGTATCTTTTCCTTCCTGACCGGCGGTTCACTg                        >  2:1007382/1‑140 (MQ=255)

                                                                                                              aTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGt                      >  1:55464/1‑140 (MQ=255)

                                                                                                              aTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGt                      >  1:2258991/1‑140 (MQ=255)

                                                                                                              aTCGCTGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGt                      >  2:258554/1‑140 (MQ=255)

                                                                                                                  cTGGGGCATTCGCGGCGT‑GCT‑GTT‑T‑T‑TTT‑GGCGGCTGGATGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTaa                  <  2:781425/140‑1 (MQ=255)

                                                                                                                  cTGGGGCATTCGCGGCGC‑GCT‑G‑A‑T‑TCTTT‑CGCGGCGGTACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTaa                  <  1:639386/140‑1 (MQ=255)

                                                                                                                   tGGGGCATTCGCGGCTC‑GCT‑G‑GCT‑T‑ATT‑GACGGCGGGCCGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGC‑GATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagc                <  1:765263/140‑1 (MQ=255)

                                                                                                                   tGGGGCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGTGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAag                 <  1:179370/140‑1 (MQ=255)

                                                                                                                    ggggCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagc                <  2:585080/140‑1 (MQ=255)

                                                                                                                    ggggCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagc                <  2:55464/140‑1 (MQ=255)

                                                                                                                    ggggCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagc                <  1:396019/140‑1 (MQ=255)

                                                                                                                    ggggCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagc                <  2:2258991/140‑1 (MQ=255)

                                                                                                                       gCATTCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAagcagc             <  2:519431/140‑1 (MQ=255)

                                                                                                                          ttCGCGGCGC‑GCT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTg          >  1:1979064/1‑140 (MQ=255)

                                                                                                                                gcgc‑gcT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGaataat    >  2:76209/1‑140 (MQ=255)

                                                                                                                                  gc‑gcT‑G‑GTT‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGc  <  1:392894/140‑1 (MQ=255)

CGTGGAGAAAAAAGAGGACGTAGATGTCGATAAAATCCTCAATCAAAGCGGTATCGCACTGGCCATTGCTGCGCTACTTTGCGCATTTATTGGCGGTATGCGTAAGGAAAATCGCTGGGGCATTCGCGGCGC‑GCT‑G‑G‑T‑T‑TTT‑GGCGGCGGGACGCTGGCTTTCCATACCTTG‑CTTTT‑TGGTATCGGCATCGTGTGCAGCATTTTGCTGATATTCCTTATCTTTTCCTTCCTGACCGGCGGTTCACTGGTTTAAGCAGCCTGAATAATGC  >  NC_000913/3856612‑3856880

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`