BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,488,579	(C)_7→8	coding (1485/1503 nt)	yjgR ←	DUF853 domain‑containing protein YjgR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,488,572	1	.	C	95.7%	559.9 / 12.8	163	coding (1492/1503 nt)	yjgR	DUF853 domain‑containing protein YjgR
Reads supporting (aligned to +/- strand): ref base . (1/6); new base C (90/66); total (91/72)
Fisher's exact test for biased strand distribution p-value = 4.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 187 total aligned reads displayed.

TTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑C‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAATATCCT  >  NC_000913/4488435‑4488706

ttattaTTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑c                                                                                                                                        <  1:1404777/140‑1 (MQ=255)

  attattGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATAATGCAGTGGGGTGGGCATGAAAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAACTCGCCTTACTTTCCTTCCT‑CT‑c‑ccc                                                                                                                                      <  2:1790731/140‑1 (MQ=255)

  attattGTTAGCAAAGTGTGCTCCGTTCATTTCTGTAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑ccc                                                                                                                                      <  2:916827/140‑1 (MQ=255)

       tGTTATCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑ccca                                                                                                                                >  2:2488163/1‑138 (MQ=255)

       tGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑ccca                                                                                                                                >  2:2447315/1‑138 (MQ=255)

        gTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTGGCCTGAATTACCTTCTT‑CT‑c‑cccc‑cccag                                                                                                                               >  1:1105487/1‑137 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAGAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  1:1342743/1‑140 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGATTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  2:1620715/1‑140 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  2:401887/1‑140 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  1:2216038/1‑140 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  1:1659035/1‑140 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑cccagca                                                                                                                             <  1:1285357/139‑6 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑cccagca                                                                                                                             >  2:1285357/1‑134 (MQ=255)

           aGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTCGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACACATCTCGCTTGAGTTCCCTTCTA‑CTCC‑CCCC‑CC‑AGCaa                                                                                                                            >  1:956866/1‑140 (MQ=38)

            gCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGATTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCaaa                                                                                                                           <  1:1620715/140‑1 (MQ=255)

              aaaGTGTGGTTCGTTCACTCCTTAAAAATAAATAAGATTTAATATATTTCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACt                                                                                                                         <  2:1143528/140‑1 (MQ=38)

              aaaGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACt                                                                                                                         >  2:1854121/1‑140 (MQ=255)

                   gtgCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCa                                                                                                                    >  2:2144780/1‑140 (MQ=255)

                   gtgCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCa                                                                                                                    >  1:1805536/1‑140 (MQ=255)

                   gtgCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCATGACTTACCTTGTT‑GTCC‑CCCC‑CC‑AGCAAACTCCCCa                                                                                                                    >  1:1061811/1‑140 (MQ=39)

                     gCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAAc                                                                                                                  <  1:2447315/140‑1 (MQ=255)

                      cTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑cccagca                                                                                                                             <  2:1650403/128‑6 (MQ=255)

                      cTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑c‑cccc‑cccagca                                                                                                                             >  1:1650403/1‑123 (MQ=255)

                          gTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCGGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAAACTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATccc                                                                                                             <  1:2488163/140‑1 (MQ=255)

                            tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAATACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCcgc                                                                                                           >  1:2378613/1‑140 (MQ=255)

                            tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTCCCTTCTT‑CT‑C‑CCCC‑CCAAGCATTCTCCCCAGCATCCcgc                                                                                                           >  2:1128755/1‑140 (MQ=39)

                            tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAAc                                                                                                                  >  1:176574/1‑133 (MQ=255)

                            tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAAc                                                                                                                  <  2:176574/133‑1 (MQ=255)

                            tCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCcgc                                                                                                           >  2:1329114/1‑140 (MQ=255)

                              aTTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGt                                                                                                         >  1:52060/1‑140 (MQ=255)

                                     aaaaaTAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCt                                                                                                  >  2:231070/1‑140 (MQ=255)

                                     aaaaaTAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCt                                                                                                  >  1:36288/1‑140 (MQ=255)

                                       aaaTAATTAAAATTCGATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGa                                                                                                <  1:1128755/140‑1 (MQ=255)

                                                          tatTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcgg                                                                             <  2:2216038/140‑1 (MQ=255)

                                                          tatTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcgg                                                                             <  1:2071567/140‑1 (MQ=255)

                                                             tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAgcgg                                                                             >  2:1490961/1‑137 (MQ=255)

                                                             tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGgcgc                                                                          >  2:2002250/1‑140 (MQ=255)

                                                             tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGgcgc                                                                          >  1:106058/1‑140 (MQ=255)

                                                             tGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGgcgc                                                                          >  2:568707/1‑140 (MQ=255)

                                                              gCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCt                                                                         <  2:294578/140‑1 (MQ=255)

                                                               cAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCtt                                                                        >  1:2294040/1‑140 (MQ=255)

                                                               cAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGAAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCtt                                                                        >  1:1609063/1‑140 (MQ=255)

                                                                aGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCttt                                                                       >  2:292550/1‑140 (MQ=255)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTGCAACCTGATTCGCCACTCGGCGAGCGGCGCTTTTg                                                                     >  2:1103716/1‑140 (MQ=39)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTg                                                                     >  1:91943/1‑140 (MQ=255)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTg                                                                     >  2:1917192/1‑140 (MQ=255)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTg                                                                     >  2:254905/1‑140 (MQ=255)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTg                                                                     >  1:2064167/1‑140 (MQ=255)

                                                                  tggggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCAGATGCGGCACTTGACGAGCGGCGCTTTTg                                                                     >  1:844789/1‑140 (MQ=39)

                                                                    gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCTTACGATCGGATTCGTCGCTTGGCGAGCGGCGCTTTTGGc                                                                   >  2:943487/1‑140 (MQ=39)

                                                                    gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGc                                                                   >  2:540406/1‑140 (MQ=255)

                                                                    gggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGc                                                                   >  1:1942654/1‑140 (MQ=255)

                                                                     ggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  <  1:485144/140‑1 (MQ=255)

                                                                     ggtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  <  1:2002250/140‑1 (MQ=255)

                                                                     ggtgggCATGATAAGACGCGCCAGCGTCGCATCAGACAACGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  <  2:1042554/140‑1 (MQ=255)

                                                                      gtgggCATGATAATACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCa                                                                 <  2:2378613/140‑1 (MQ=255)

                                                                      gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCa                                                                 <  2:1942654/140‑1 (MQ=255)

                                                                      gtgggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCa                                                                 <  1:1836672/140‑1 (MQ=255)

                                                                         ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCt                                                                         >  2:212275/1‑129 (MQ=255)

                                                                         ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCt                                                                         <  1:212275/129‑1 (MQ=255)

                                                                         ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTg                                                              >  1:1328240/1‑140 (MQ=255)

                                                                         ggCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTg                                                              >  1:620735/1‑140 (MQ=255)

                                                                              gATAAGACGCGCCAGTGTCGCAGCAAGCAGAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGc                                                         <  1:1090259/140‑1 (MQ=255)

                                                                              gATAAGACGCGCCAGCGTCGCAGCAGGCAAAGAGAAATCCCGCCTGACGTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGc                                                         <  2:1061811/140‑1 (MQ=255)

                                                                               aTAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGca                                                        <  1:2144780/140‑1 (MQ=255)

                                                                                tAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGAAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcac                                                       <  2:1609063/140‑1 (MQ=255)

                                                                                   gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  >  1:2243225/1‑126 (MQ=255)

                                                                                   gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGcc                                                                  <  2:2243225/126‑1 (MQ=255)

                                                                                   gACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccac                                                    >  1:1224380/1‑140 (MQ=255)

                                                                                     cgcgCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGcaccacac                                                  <  2:532287/140‑1 (MQ=255)

                                                                                        gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCAt                                               >  1:509823/1‑140 (MQ=255)

                                                                                        gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCAt                                               >  1:2395075/1‑140 (MQ=255)

                                                                                        gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGACTCGTCACTTGGCAAGCGGCGCTTTTGGCCAATGTTTGCACCACACCAt                                               >  2:844840/1‑140 (MQ=255)

                                                                                        gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACCCCCCAAAAACCCGCGTACAATCTGATTCGTTCTCTGGCAACGGTCCCTTTTGTCCATGGTTTGAACCACACCAt                                               >  2:1314354/1‑140 (MQ=255)

                                                                                        gcCAGCGTCGCATCAGGCAAAGACAAATCTCGCCAGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCAt                                               >  2:307240/1‑140 (MQ=255)

                                                                                                       ggCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCcgccgc                                >  1:32958/1‑140 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCGTGACGTACCTTCTC‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:961163/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACTTACCTTCTTCCC‑C‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:425216/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  1:289868/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  1:409187/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  1:1726041/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑CGCCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:1088504/140‑1 (MQ=255)

                                                                                                        gCAAAGACAAATCTCGCCTGACGTACCTTCTT‑CC‑C‑CCCCTCC‑AGCAAACTCACCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:1105487/140‑1 (MQ=255)

                                                                                                           aaGACAAATCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTgg                            <  2:14003/140‑1 (MQ=255)

                                                                                                                aaaTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCgg                       >  2:1725187/1‑140 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACAGCATCTTGCTTCCCGCCGCGTGGTCCGGTa                     >  1:684904/1‑140 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               <  2:1357096/130‑1 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCcgcg                               >  1:1357096/1‑130 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTa                     >  2:2175204/1‑140 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTa                     >  1:446741/1‑140 (MQ=255)

                                                                                                                  aTCTCGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTCCACCACACCATCTTGCTTCCCCCCGCGTGGTCCGGTa                     >  1:963651/1‑140 (MQ=255)

                                                                                                                     tcGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTg                  <  1:540406/140‑1 (MQ=255)

                                                                                                                     tcGCCTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTg                  <  1:47990/140‑1 (MQ=255)

                                                                                                                     ccaccTGACTCACTTTCTTCCG‑C‑CCCG‑CC‑ACCAACGTCCCCAACATCCCGCGTACAACCTTATTCGTCACTTGGCGAGCGGCGCTTTTCGCCATTGTTAGCACCACCCCATCTTTCTACACGCCGCGTAGTCAGgtggtg                  <  2:512797/137‑1 (MQ=255)

                                                                                                                        ccTGACTTACCTTCTTCCC‑C‑CCCC‑CC‑AGCAACCTCGCCATCATCCCGCCTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCg               <  2:684904/140‑1 (MQ=255)

                                                                                                                         cTGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGa              <  2:480525/140‑1 (MQ=255)

                                                                                                                          tGACTTACCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGaa             <  2:2064167/140‑1 (MQ=255)

                                                                                                                                aCCTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGACATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAat       <  2:36288/140‑1 (MQ=255)

                                                                                                                                 ccTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAata      <  1:1329114/140‑1 (MQ=255)

                                                                                                                                 ccTTCTT‑CTCC‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAata      <  1:1917192/140‑1 (MQ=255)

TTATTATTGTTAGCAAAGTGTGCTTCGTTCATTCCTGAAAAATAATTAAAATTCAATATATTGCAGTGGGGTGGGCATGATAAGACGCGCCAGCGTCGCATCAGGCAAAGACAAATCTCGCCTGACTTACCTTCTT‑CT‑C‑CCCC‑CC‑AGCAAACTCCCCAACATCCCGCGTACAATCTGATTCGTCACTTGGCGAGCGGCGCTTTTGGCCATTGTTTGCACCACACCATCTTTCTTCCCGCCGCGTGGTCCGGTAGTGCCGAACAAAATATCCT  >  NC_000913/4488435‑4488706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`