BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,458,625	(G)_6→7	coding (362/789 nt)	paaG →	putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,458,619	1	.	G	93.5%	347.4 / 14.0	107	coding (356/789 nt)	paaG	putative ring 1,2‑epoxyphenylacetyl‑CoA isomerase (oxepin‑CoA forming)
Reads supporting (aligned to +/- strand): ref base . (4/3); new base G (51/49); total (55/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 117 total aligned reads displayed.

CACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGG  >  NC_000913/1458482‑1458753

cacaGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAAcactggcac‑t‑‑g‑g                                                                                                                                         <  1:523275/140‑1 (MQ=255)

 acaGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑g‑gg                                                                                                                                        >  1:143901/1‑140 (MQ=255)

        ccGCACCGGATTTAGTGATGTCAGTTGAACGTTTCTAAAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑g‑gg‑ggggcga                                                                                                                                <  1:755635/140‑5 (MQ=255)

        ccGCACCGGATTTAGCGATGTCTGTCGAATGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑g‑gg‑ggggcga                                                                                                                                <  1:1057379/140‑5 (MQ=255)

         cGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑g‑gg‑ggggcgac                                                                                                                               <  2:1801372/140‑6 (MQ=255)

             ccGGATTTAGGGATGTCAGTTGTACGTTTCTTTAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCg                                                                                                                          <  2:1026442/140‑1 (MQ=255)

               ggATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGtt                                                                                                                        >  2:2443709/1‑140 (MQ=255)

                          tGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCg                                                                                                             >  1:1999390/1‑140 (MQ=255)

                          tGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCg                                                                                                             >  2:1261276/1‑140 (MQ=255)

                           gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACTCTGGCACGG‑‑G‑GG‑GGGCG‑‑ACATTGTTATTGCTGCCCGt                                                                                                            >  1:721892/1‑140 (MQ=255)

                           gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGt                                                                                                            >  2:239156/1‑140 (MQ=255)

                           gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGt                                                                                                            >  2:531211/1‑140 (MQ=255)

                           gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGt                                                                                                            >  1:454512/1‑140 (MQ=255)

                           gTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑A‑‑G‑GGCGGCCG‑‑ACATTGTTATTGCTTCCCGt                                                                                                            >  1:1201571/1‑140 (MQ=255)

                               gTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAg                                                                                                        >  2:445309/1‑140 (MQ=255)

                                   aaCGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCaaa                                                                                                    <  1:1675212/140‑1 (MQ=255)

                                   aaCGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCaaa                                                                                                    <  1:1998391/140‑1 (MQ=255)

                                     cGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAAt                                                                                                  <  1:2405308/140‑1 (MQ=255)

                                        ttCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCg                                                                                               >  2:2359746/1‑140 (MQ=255)

                                          cTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTc                                                                                             >  1:1636447/1‑140 (MQ=255)

                                           tataACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                            <  2:1683492/140‑1 (MQ=255)

                                           tataACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCa                                                                                            <  1:1297314/140‑1 (MQ=255)

                                              aaCCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATgg                                                                                         <  2:1370715/140‑1 (MQ=255)

                                               aCCCACTGGTACGTCCCCTGGCCAACCTGTCAAAACCTGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGc                                                                                        <  1:690718/140‑1 (MQ=255)

                                                cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       <  2:143901/140‑1 (MQ=255)

                                                cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       <  1:16231/140‑1 (MQ=255)

                                                cccACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       <  1:445309/140‑1 (MQ=255)

                                                  cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       >  1:2033746/1‑138 (MQ=255)

                                                  cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       <  2:2033746/138‑1 (MQ=255)

                                                  cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGtt                                                                                     >  2:2284957/1‑140 (MQ=255)

                                                  cACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGtt                                                                                     >  1:2314600/1‑140 (MQ=255)

                                                   cagggTACGTCGCCTGGCAAATTTGGCAAAACCGGTTTTCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGttt                                                                                    <  1:1199809/137‑1 (MQ=255)

                                                   aCTGGTACGTCGCCTGGGAAAACTGCCAAAATCGGGGATCTGTGCAGTAAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGC‑AAATTCGTCATGGCGTTTa                                                                                   <  2:755666/140‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCg                                                                                       <  2:2074370/136‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGtttt                                                                                   >  2:575794/1‑139 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   >  2:1949677/1‑140 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  2:1999390/140‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  1:2443709/140‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   >  2:627720/1‑140 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  1:2115400/140‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  1:1303682/140‑1 (MQ=255)

                                                    cTGGTACGTCGCCTGGATAAACGGCCAAAACCGGTGATCGTTGCAGTCTATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  2:1121344/140‑1 (MQ=255)

                                                     tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCTCGTTCAGCAAAATCCGTCATGGCGTTTAg                                                                                  >  1:1071204/1‑140 (MQ=255)

                                                     tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   >  2:87777/1‑139 (MQ=255)

                                                     tGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTa                                                                                   <  1:87777/139‑1 (MQ=255)

                                                          cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                             >  2:531815/1‑140 (MQ=255)

                                                          cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGAAAGt                                                                             >  2:2057907/1‑140 (MQ=255)

                                                          cGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCATTCAGCAAAATTCGTCATGGCGTTTAGTAAGt                                                                             >  2:2412703/1‑140 (MQ=255)

                                                           gTCGCCTGGCAAAACTGGCAAACCCGGTGATTTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                            <  2:923705/140‑1 (MQ=255)

                                                           gTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                            <  2:1636447/140‑1 (MQ=255)

                                                           gTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                            <  2:1670529/140‑1 (MQ=255)

                                                           gTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                            <  1:2397579/140‑1 (MQ=255)

                                                            tCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                                                                           <  1:239156/140‑1 (MQ=255)

                                                            tCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTa                                                                           >  2:380846/1‑140 (MQ=255)

                                                              gCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAgg                                                                         <  1:2111701/140‑1 (MQ=255)

                                                               ccTGGCAAAACTGCCAAAACCGGTGATCTTTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAGAATACGGCATGGCGTTTAGTAAGTTAGGc                                                                        >  1:1121344/1‑140 (MQ=255)

                                                               ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                                                                        >  1:1942691/1‑140 (MQ=255)

                                                               ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                                                                        >  2:2452544/1‑140 (MQ=255)

                                                               ccTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGc                                                                        >  2:7841/1‑140 (MQ=255)

                                                                cTGGCAAACCTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCCGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCGACACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAgg                                                                         <  2:2162457/140‑1 (MQ=255)

                                                                cTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCt                                                                       <  2:455085/140‑1 (MQ=255)

                                                                  ggCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTa                                                                     >  1:1872883/1‑140 (MQ=255)

                                                                   gCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGtt                                                                            >  1:2039041/1‑132 (MQ=255)

                                                                     aaaaCTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATa                                                                  <  1:1536305/140‑1 (MQ=255)

                                                                     aaaaCTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATa                                                                  <  1:531815/140‑1 (MQ=255)

                                                                     aaaaCTGCCAAAACCGGTGATCCGTGCAGTCTATGCCGTTGCGGCAGGTGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATa                                                                  <  2:656459/140‑1 (MQ=255)

                                                                         cTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCACAATACGTGATGGCGTTTAGTAGGTTAGGCTTAATACCCg                                                              >  2:942477/1‑140 (MQ=255)

                                                                            ccAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGAtt                                                           <  1:7841/140‑1 (MQ=255)

                                                                             cAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTg                                                          <  1:2000213/140‑1 (MQ=255)

                                                                             cAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTg                                                          >  1:2161766/1‑140 (MQ=255)

                                                                             cAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTg                                                          >  1:474045/1‑140 (MQ=255)

                                                                                 aCCGGTGATCTGTGCGGTCAATGGCGTGGTGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                      <  1:942477/140‑1 (MQ=255)

                                                                                 aCCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                      <  2:1872883/140‑1 (MQ=255)

                                                                                 aCCGGTGACCTGTGCAGTCAATCGCGTGGCAACAGCGGCCGGCGCAACGCTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGt                                                      <  2:1213018/140‑1 (MQ=255)

                                                                                      tGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAAc                                                 <  2:1737900/140‑1 (MQ=255)

                                                                                      tGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAAc                                                 <  1:1937930/140‑1 (MQ=255)

                                                                                      tGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAAc                                                 <  1:531211/140‑1 (MQ=255)

                                                                                       gATCTGTGCAGTCAACGGCGTGACGCCAGTGGCAGGCCCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAAcc                                                <  1:693740/140‑1 (MQ=255)

                                                                                           tgtgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑GAGG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCGTAATACCCGATTGCGGTGGAACCTGGt                                            <  2:1939651/140‑1 (MQ=255)

                                                                                             tgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAAGACACGATTGCGGTGGAACCTGGTTa                                          >  1:987234/1‑140 (MQ=255)

                                                                                             tgCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAACACGCGATTGGGGTGGAACCTGGTTa                                          >  1:1178161/1‑140 (MQ=255)

                                                                                                 gTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGc                                      >  2:2110887/1‑140 (MQ=255)

                                                                                                  tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGcc                                     >  2:2398615/1‑140 (MQ=255)

                                                                                                  tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGcc                                     >  1:1492954/1‑140 (MQ=255)

                                                                                                  tCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGcc                                     >  2:2039354/1‑140 (MQ=255)

                                                                                                    aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTTTTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGGTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAc                                   >  2:329900/1‑140 (MQ=255)

                                                                                                    aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAc                                   >  2:310817/1‑140 (MQ=255)

                                                                                                    aaTGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAc                                   >  1:2431304/1‑140 (MQ=255)

                                                                                                      tggcgtggcgGCAGGCGCAGGCGCACCACTGGCAC‑T‑‑GTGG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCAcgc                                 <  1:781804/140‑1 (MQ=255)

                                                                                                         cgtggcgGCAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGtt                              >  2:2165861/1‑140 (MQ=255)

                                                                                                            ggcggcAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGcc                           >  2:1411270/1‑140 (MQ=255)

                                                                                                              cggcAGGGGCAGGCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCgg                         >  2:1942722/1‑140 (MQ=255)

                                                                                                                       caggCGCAACACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCATTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg                <  1:2412703/140‑1 (MQ=255)

                                                                                                                        aggCGCAACACTGGCAC‑TGGG‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg                <  1:1659866/140‑1 (MQ=255)

                                                                                                                              aaCACTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATg         >  2:1447059/1‑140 (MQ=255)

                                                                                                                               acacTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg                <  1:2323472/132‑1 (MQ=255)

                                                                                                                               acacTGGCAC‑TG‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg                >  2:2323472/1‑132 (MQ=255)

                                                                                                                                cactggcac‑tg‑g‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATggg       >  1:2329336/1‑140 (MQ=255)

                                                                                                                                    ggcac‑tg‑g‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAgcgcg                <  2:1627187/127‑1 (MQ=255)

                                                                                                                                     gcac‑tg‑g‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTgg  >  1:1822050/1‑140 (MQ=255)

CACAGGCCCCGCACCGGATTTAGGGATGTCAGTTGAACGTTTCTATAACCCACTGGTACGTCGCCTGGCAAAACTGCCAAAACCGGTGATCTGTGCAGTCAATGGCGTGGCGGCAGGGGCAGGCGCAACACTGGCAC‑T‑‑G‑GG‑GGGCG‑‑ACATCGTTATTGCTGCCCGTTCAGCAAAATTCGTCATGGCGTTTAGTAAGTTAGGCTTAATACCCGATTGCGGTGGAACCTGGTTACTGCCACGCGTTGCCGGACGAGCGCGCGCCATGGGGCTGG  >  NC_000913/1458482‑1458753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`