BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	770,007	Δ36 bp	coding (2030‑2065/2634 nt)	mngB →	alpha‑mannosidase

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	770007	770042	36	98 [0]	[0] 100	mngB	alpha‑mannosidase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 770006	0 (0.000)	97 (0.660)	61/276	0.6	100%	coding (2029/2634 nt)	mngB	alpha‑mannosidase
*	?	NC_000913	770043 =	0 (0.000)	97 (0.660)	61/276	0.6	100%	coding (2066/2634 nt)	mngB	alpha‑mannosidase

TGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAGCGCCGGTTCCGGTATGGAATATGCTCAACTATGTTGCCTTACAGGAAGGGCGTAACGGCATGGCTGTCTTTAGCGAAGGGTTACGTGAATTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGT  >  NC_000913/769905‑770182

tggcagatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtatt                                                                                                                                            <  2:1512153‑M2/2‑1 (MQ=255)

tggcagatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtatt                                                                                                                                            >  1:1825005‑M2/139‑140 (MQ=255)

tggcagatacgcagtttcgttcgctaacgcgccccgtgaccgacagtgtaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtatt                                                                                                                                            <  2:811045‑M2/2‑1 (MQ=255)

 ggcagatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTg                                                                                                                                           <  2:1777842‑M2/3‑1 (MQ=255)

  gcagatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttcgggtatggaatatgctcgactatgttgcctttcaggaagggcgtaTTGa                                                                                                                                          >  2:662818‑M2/137‑140 (MQ=255)

   cagatacgcagtttgcttcgcttacccgccccgtgaatgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGaa                                                                                                                                         <  1:662818‑M2/5‑1 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtatt                                                                                                                                            <  1:2467799‑M2/2‑1 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtatt                                                                                                                                            >  2:2467799‑M2/134‑135 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       >  2:2358362‑M2/134‑140 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       >  2:1875210‑M2/134‑140 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       <  1:2436311‑M2/7‑1 (MQ=255)

     gatacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       <  2:1246722‑M2/7‑1 (MQ=255)

     gatacgcagtttggtgcgctaacgctcccggtgaacgccagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       <  1:740522‑M2/7‑1 (MQ=255)

     gatacgcagtttggtacgctaaggcgcccagtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGt                                                                                                                                       <  1:923049‑M2/7‑1 (MQ=255)

      atacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTc                                                                                                                                      <  1:2033128‑M2/8‑1 (MQ=255)

       tacgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCa                                                                                                                                     >  1:165210‑M2/132‑140 (MQ=255)

        acgcagtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCAt                                                                                                                                    <  1:1988895‑M2/10‑1 (MQ=255)

             gtttggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTg                                                                                                                               <  1:195327‑M2/15‑1 (MQ=255)

              tttggttcgctaacacgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGaa                                                                                                                                         >  2:2419845‑M2/125‑129 (MQ=255)

              tttggttcgctaacacgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGaa                                                                                                                                         <  1:2419845‑M2/5‑1 (MQ=255)

                tggttcgctaacgcgccccgtgcacgacactgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTgaag                                                                                                                            <  1:1110406‑M2/18‑1 (MQ=255)

                tggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTgaag                                                                                                                            <  2:1231900‑M2/18‑1 (MQ=255)

                tggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTgaag                                                                                                                            <  2:1825005‑M2/18‑1 (MQ=255)

                 ggttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTgaaga                                                                                                                           <  2:523850‑M2/19‑1 (MQ=255)

                  gttcgctaacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTgaagag                                                                                                                          >  2:407676‑M2/121‑140 (MQ=255)

                        taacgcgccccgtgagcgacagagcgatgaacaactgacagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGaaa                                                                                                                    <  1:1101932‑M2/26‑1 (MQ=255)

                        taacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggcaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGaaa                                                                                                                    >  2:1343856‑M2/115‑140 (MQ=255)

                        taacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacagtaagagcgtaTTGGAGTCGGCGGTGAAGAGAAGaaa                                                                                                                    >  2:1110406‑M2/115‑140 (MQ=255)

                        taacgcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGaaa                                                                                                                    >  1:627281‑M2/115‑140 (MQ=255)

                            gcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCt                                                                                                                <  2:627281‑M2/30‑1 (MQ=255)

                            gcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCt                                                                                                                <  1:1702716‑M2/30‑1 (MQ=255)

                            gcgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCt                                                                                                                <  2:165210‑M2/30‑1 (MQ=255)

                             cgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCtt                                                                                                               <  1:2301269‑M2/31‑1 (MQ=255)

                             cgccccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCtt                                                                                                               <  1:1343856‑M2/31‑1 (MQ=255)

                                cccgtgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGc                                                                                                            <  1:396283‑M2/34‑1 (MQ=255)

                                    tgaacgacagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCAtt                                                                                                        >  2:198435‑M2/103‑140 (MQ=255)

                                    tgaacgacagtgcaatgaacaaatggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCAtt                                                                                                        <  1:1216102‑M2/38‑1 (MQ=255)

                                           cagtgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTtgc                                                                                                 <  2:527801‑M2/45‑1 (MQ=255)

                                              tgcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTtgctgc                                                                                              >  2:2140138‑M2/93‑140 (MQ=255)

                                               gcaatgaacaactggcagcaagtagtctgaaaagaagtgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcg                                                                                             <  1:808109‑M2/49‑1 (MQ=255)

                                               gcaatgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcg                                                                                             <  1:2047573‑M2/49‑1 (MQ=255)

                                                  atgaacaactggcagcaagaaggctggaaagaagcgctggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcgtgg                                                                                          <  1:1069798‑M2/52‑1 (MQ=255)

                                                  atgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcgtgg                                                                                          >  2:1955112‑M2/89‑140 (MQ=255)

                                                  atgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcgtgg                                                                                          <  1:2024357‑M2/52‑1 (MQ=255)

                                                   tgaacaactggcagctagaaggttgcaaacaagcgcctgttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcgtggc                                                                                         <  1:673615‑M2/53‑1 (MQ=255)

                                                   tgaacaactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTgcgtggc                                                                                         >  2:1513164‑M2/88‑140 (MQ=255)

                                                        aactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTggg                                                                                    >  2:193354‑M2/83‑140 (MQ=255)

                                                        aactggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTggg                                                                                    >  2:2335904‑M2/83‑140 (MQ=255)

                                                            ggcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTa                                                                                <  1:2335904‑M2/62‑1 (MQ=255)

                                                            ggcagcaagaaggctagaaagacgcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTa                                                                                <  1:1146481‑M2/62‑1 (MQ=255)

                                                             gcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTAc                                                                               >  2:1539143‑M2/78‑140 (MQ=255)

                                                             gcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTAc                                                                               >  1:2454235‑M2/78‑140 (MQ=255)

                                                             gcagcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTAc                                                                               <  1:303455‑M2/63‑1 (MQ=255)

                                                               agcaagaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTg                                                                             >  2:2084730‑M2/76‑140 (MQ=255)

                                                                    gaaggctggaaagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCaa                                                                        >  1:1295898‑M2/71‑140 (MQ=255)

                                                                     aaggctggaaagaagagccggtaccagtaaggaatatactcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCaaa                                                                       <  1:1202377‑M2/71‑1 (MQ=255)

                                                                            gaaagaagcgccggtaccggtatggcatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATc                                                                <  1:1048979‑M2/78‑1 (MQ=255)

                                                                              aagaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTg                                                              <  1:2140138‑M2/80‑1 (MQ=255)

                                                                                gaagcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCt                                                            >  2:1719231‑M2/59‑140 (MQ=255)

                                                                                  agcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCttt                                                          >  1:2476887‑M2/57‑140 (MQ=255)

                                                                                   gcgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCtttt                                                         <  2:171947‑M2/85‑1 (MQ=255)

                                                                                    cgccggttccggtatggaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTa                                                        >  1:1348283‑M2/55‑140 (MQ=255)

                                                                                     gccggttccggtatgtaatatgctctactctgttgccatacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTaa                                                       <  1:728908‑M2/87‑1 (MQ=255)

                                                                                                    gaatatgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCtt                                        <  1:1467245‑M2/102‑1 (MQ=255)

                                                                                                         tgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCATAGAAGATGTGCTTTGAAGGCCAGGGCGGCCTTCGGGa                                   >  1:681991‑M2/34‑140 (MQ=255)

                                                                                                         tgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGa                                   <  1:1875210‑M2/107‑1 (MQ=255)

                                                                                                         tgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGa                                   >  1:1770725‑M2/34‑140 (MQ=255)

                                                                                                         tgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGa                                   <  1:1513164‑M2/107‑1 (MQ=255)

                                                                                                         tgctcaactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGa                                   >  2:2408228‑M2/34‑140 (MQ=255)

                                                                                                             caactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTa                               <  1:2358362‑M2/111‑1 (MQ=255)

                                                                                                              aactatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTaa                              >  1:42351‑M2/29‑140 (MQ=255)

                                                                                                                ctatgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTaaaa                            >  1:2207673‑M2/27‑140 (MQ=255)

                                                                                                                   tgttgccttacaggaagtgcgtaTTGAAGTCTTCGGTGATGAGAAGAATACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGc                         <  2:681991‑M2/117‑1 (MQ=255)

                                                                                                                   tgttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGc                         >  2:26789‑M2/24‑140 (MQ=255)

                                                                                                                     ttgccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCT‑CTTTTAAGGCCTGGTCGCCCTTCGTGAATTAAAATGCCAg                      >  1:908322‑M2/22‑140 (MQ=255)

                                                                                                                        ccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTc                    >  1:115317‑M2/19‑140 (MQ=255)

                                                                                                                        ccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTc                    >  2:621164‑M2/19‑140 (MQ=255)

                                                                                                                        ccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTc                    >  1:1537987‑M2/19‑140 (MQ=255)

                                                                                                                        ccttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTc                    >  1:187452‑M2/19‑140 (MQ=255)

                                                                                                                         cttacaggaagggcgtaTTGAAATCATCGGTCAAGAGAACAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCT‑CTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTccc                  <  2:908322‑M2/123‑1 (MQ=255)

                                                                                                                          ttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTccc                  <  1:489614‑M2/124‑1 (MQ=255)

                                                                                                                          ttacaggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTccc                  <  2:2454235‑M2/124‑1 (MQ=255)

                                                                                                                             caggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGa               >  2:1549542‑M2/14‑140 (MQ=255)

                                                                                                                             caggaagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGa               >  2:1840596‑M2/14‑140 (MQ=255)

                                                                                                                             caggaagggcgcaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGa               >  2:2461860‑M2/14‑140 (MQ=255)

                                                                                                                                 aagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTca           <  1:193354‑M2/131‑1 (MQ=255)

                                                                                                                                 aagggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTca           <  1:1955112‑M2/131‑1 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCGTCGGTGAAGAGAAAAAAATCTTCGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          <  2:1154954‑M2/132‑1 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          >  2:2241918‑M2/9‑140 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          >  2:2032744‑M2/9‑140 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          >  2:324441‑M2/9‑140 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          >  2:202893‑M2/9‑140 (MQ=255)

                                                                                                                                  agggcgtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcac          >  2:1752810‑M2/9‑140 (MQ=255)

                                                                                                                                   gggcgtaTTGAAGTCATCGGTGCAGAGAAGAAAACTTTTCCCATTACGGTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcaca         <  2:866380‑M2/133‑1 (MQ=255)

                                                                                                                                   gggcgtaTTGAAGTCATCGGTGAAGCGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTcaca         <  1:1094707‑M2/133‑1 (MQ=255)

                                                                                                                                       gtaTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCGTTCGGGATTTAGAAGGCCAGTGCCGGACTCACAACTa     >  1:657105‑M2/4‑140 (MQ=255)

                                                                                                                                         aTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACg   <  1:407676‑M2/139‑1 (MQ=255)

                                                                                                                                          ttGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGt  <  1:1539143/140‑1 (MQ=255)

                                                                                                                                          ttGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGt  <  1:324441/140‑1 (MQ=255)

                                                                                                                                          ttGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGt  <  2:1770725/140‑1 (MQ=255)

TGAACGACAGTGCAATGAACAACTGGCAGCAAGAAGGCTGGAAAGAAGCGCCGGTTCCGGTATGGAATATGCTCAACTATGTTGCCTTACAGGAAGGGCGTAACGGCATGGCTGTCTTTAGCGAAGGGTTACGTGAATTTGAAGTCATCGGTGAAGAGAAGAAAACCTTTGCCATTACGTTGCTGCGTGGCGTGGGCTTACTGGGCAAAGAAGATCTGCTTTTAAGGCCTGGGCGGCCTTCGGGAATTAAAATGCCAGTCCCGGACTCACAACTACGT  >  NC_000913/769905‑770182

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`