Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,569,312	A→G	V393A (GTT→GCT)	gadC ←	L‑glutamate:4‑aminobutyrate antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,569,312	0	A	G	100.0%	281.5 / NA	78	V393A (GTT→GCT)	gadC	L‑glutamate:4‑aminobutyrate antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/51);  total (27/51)

TGGATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGA  >  NC_000913/1569177‑1569438                                                                                                                                        |                                                                                                                               tGGATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCaa                                                                                                                            <  2:1858689/140‑1 (MQ=255) tGGATGTTATCCGGCGACAGGAAGGAAACAAAAAAGGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCaa                                                                                                                            <  2:1077040/140‑1 (MQ=255)  ggATGTTATCCGGCGTCAGGAAGGAGACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAac                                                                                                                           <  1:1182590/140‑1 (MQ=255)  ggATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGAGCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAac                                                                                                                           <  2:423267/140‑1 (MQ=255)  ggATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAac                                                                                                                           <  1:1441737/140‑1 (MQ=255)   gATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAaca                                                                                                                          >  1:434806/1‑140 (MQ=255)    aTGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAacac                                                                                                                         >  1:106275/1‑140 (MQ=255)        tATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATg                                                                                                                     >  2:425489/1‑140 (MQ=255)         atcaggcggcAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    >  1:2016113/5‑140 (MQ=255)         aTCCGGCGGCAGGAAGGCAACAATAAACGCCCTAATTGAAGTCAGCAGTCCGTCAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  2:742982/140‑1 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCTACAATTGCCACCACCAGTTTCACCCCTTTACCACCAGGGCTATTAAATTTGCCTCTTCAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    >  2:1205474/1‑140 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGTTATTAAATTTGCTTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    >  2:833116/1‑140 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  2:2129593/140‑1 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    >  1:470586/1‑140 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  1:1457795/140‑1 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  1:1623739/140‑1 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  2:434806/140‑1 (MQ=255)         aTCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGt                                                                                                                    <  1:2350244/140‑1 (MQ=255)             ggcggcAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGcc                                                                                                                >  2:2193503/1‑140 (MQ=255)              gcggcAGGAAGGAAACACTAAATGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCa                                                                                                               <  2:668915/140‑1 (MQ=255)              gcggcAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCa                                                                                                               <  2:2016113/140‑1 (MQ=255)                ggcAGGAAGGAAACAATAAACGTCATAATTGCAGTCAGCTGACCGACACTTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAAt                                                                                                             <  2:707317/140‑1 (MQ=255)                 gcAGGAAGGAAACAACAAACGCTATCATTTAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATa                                                                                                            <  1:754800/140‑1 (MQ=255)                  cAGGAAGGAAACAATTAACGCCCTAATTGAAGTCAGCCGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATaa                                                                                                           <  1:1205474/140‑1 (MQ=255)                   aggaaggaAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATaaa                                                                                                          <  2:2404622/140‑1 (MQ=255)                   aggaaggaAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATaaa                                                                                                          <  2:2290971/140‑1 (MQ=255)                    ggaaggaaACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAAc                                                                                                         <  1:200316/140‑1 (MQ=255)                       aggaaACAATAAACGCCATAATCGAAGTCAGCAGACCGACAATTGCCAAGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGc                                                                                                      <  2:997178/140‑1 (MQ=255)                        ggaaACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCa                                                                                                     <  2:1771108/140‑1 (MQ=255)                        ggaaACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCa                                                                                                     <  1:141685/140‑1 (MQ=255)                         gaaACAATAAACGCCATGATTGTAGTCAGCATACCGACATTTGCCACGTCCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCAt                                                                                                    <  2:797751/140‑1 (MQ=255)                         gaaACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCAt                                                                                                    <  2:106275/140‑1 (MQ=255)                           aaCAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGa                                                                                                  <  1:425489/140‑1 (MQ=255)                               aTAAACGCCATAAGTGAAGTAAGCAGACCGACAATTGCCACGTCCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATa                                                                                              <  2:2376571/140‑1 (MQ=255)                                  aaCGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGc                                                                                           <  1:1056682/140‑1 (MQ=255)                                  aaCGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACAACCAGGGATATTAAATGTACGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGc                                                                                           <  2:1438067/140‑1 (MQ=255)                                   aCGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGca                                                                                          <  2:425161/140‑1 (MQ=255)                                   aCGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGca                                                                                          <  1:2362460/140‑1 (MQ=255)                                    cGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGcac                                                                                         <  2:470586/140‑1 (MQ=255)                                    cGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGcac                                                                                         <  2:1583911/140‑1 (MQ=255)                                     gCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGcaca                                                                                        <  1:2426846/140‑1 (MQ=255)                                        aTAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAg                                                                                     >  2:1816797/1‑140 (MQ=255)                                        aTAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAg                                                                                     >  2:1297275/1‑140 (MQ=255)                                        aTAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGAAGCCAATAGACAGCATGAAATAAGCACACAg                                                                                     >  2:1048782/1‑140 (MQ=255)                                               aaGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAAATAAATc                                                                              >  2:521018/1‑140 (MQ=255)                                                 gTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATcac                                                                            >  1:1949646/1‑140 (MQ=255)                                                       agACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGt                                                                      <  1:1517723/140‑1 (MQ=255)                                                                   gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGATATAAGCAGACAGATAATTCACAACCGTCAGCGCCAGTGc                                                          >  2:822938/1‑140 (MQ=255)                                                                   gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATcacca                                                                          >  2:1239135/1‑124 (MQ=255)                                                                   gCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATcacca                                                                          <  1:1239135/124‑1 (MQ=255)                                                                     cACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGa                                                        <  2:1715030/140‑1 (MQ=255)                                                                     cACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGa                                                        <  1:1816797/140‑1 (MQ=255)                                                                          ccAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATccgg                                                   <  1:2193503/140‑4 (MQ=255)                                                                           cAGTTTCACCCCTTTACCACCATGGCTATAAAATGTGTGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCagga                                                  <  1:822938/140‑1 (MQ=255)                                                                           cAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAAATAAATCACCACCGTCAGCGCCAGTGCGATCagga                                                  <  1:521018/140‑1 (MQ=255)                                                                             gTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCaggaag                                                <  2:1941998/140‑1 (MQ=255)                                                                               ttCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCaggaagga                                              <  1:1297275/140‑1 (MQ=255)                                                                                    cccTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCTAGTGCGATCAGGAAGGACAtgt                                         >  1:1176483/1‑140 (MQ=255)                                                                                     ccTTTACCACCAGGGGTATTAAATGAGCGTTTTAAGTTAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACAtgtt                                        <  1:833116/140‑1 (MQ=255)                                                                                        ttACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATgttgtt                                     >  2:2246744/1‑140 (MQ=255)                                                                                        ttACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATgttgtt                                     >  1:2120509/1‑140 (MQ=255)                                                                                        ttACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCAACACCGTCCGCGCCAGTACGATGAGGAAGGACATgttgtt                                     >  2:962164/1‑140 (MQ=255)                                                                                                      atTAAATGTGCGTTTCAAGTCACGACGTTGAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTAt                       <  1:1048782/140‑1 (MQ=255)                                                                                                       ttAAATGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTAtt                      >  1:1653928/1‑140 (MQ=255)                                                                                                         aaaTGTGCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTgg                    <  1:1805876/140‑1 (MQ=255)                                                                                                         aaaTGTGCGTTTTAACTCAGGATGTATAACAGCCAACTCAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTgg                    <  1:655429/140‑1 (MQ=255)                                                                                                            tgtgCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGa                 >  2:2042466/1‑140 (MQ=255)                                                                                                            tgtgCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGa                 >  1:301270/1‑140 (MQ=255)                                                                                                              tgCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg               >  1:1990467/1‑140 (MQ=255)                                                                                                              tgCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg               >  2:2330516/1‑140 (MQ=255)                                                                                                               gCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg               <  2:2384217/139‑1 (MQ=255)                                                                                                               gCGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAgg               >  1:2384217/1‑139 (MQ=255)                                                                                                                cGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTg                  >  2:2287464/1‑135 (MQ=255)                                                                                                                cGTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTg                  <  1:2287464/135‑1 (MQ=255)                                                                                                                cGTTTTAAGTAAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgat             >  1:1341573/1‑140 (MQ=255)                                                                                                                 gTTTTAAGTCAGGATGTTTAAGAGCCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGgatg            >  2:252574/1‑140 (MQ=255)                                                                                                                      aaGTCAGGATGTTTAGGAGCCACCAGAATATAGCCAACAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCaa       <  1:724698/140‑1 (MQ=255)                                                                                                                           aGGATGTTTAAGAGCCAACACAGTGCAGCCAATAAACCGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGa  <  1:962164/140‑1 (MQ=255)                                                                                                                           aGGATGTTTAAGAGCCAACACACTGTAGCCAGTAACCAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGa  <  2:943894/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               TGGATGTTATCCGGCGGCAGGAAGGAAACAATAAACGCCATAATTGAAGTCAGCAGACCGACAATTGCCACGACCAGTTTCACCCCTTTACCACCAGGGATATTAAATGTGCGTTTTAAGTCAGGATGTTTAAGAACCAACACAATGTAGCCAATAAACAGCATGAAATAAGCACACAGATAAATCACCACCGTCAGCGCCAGTGCGATCAGGAAGGACATGTTGTTACCGCCACCGGTATTGGTGAGGATGATCAACGCGA  >  NC_000913/1569177‑1569438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑