Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,281,874	T→C	F415L (TTT→CTT)	radD →	putative DNA repair helicase RadD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,281,874	0	T	C	100.0%	240.3 / NA	71	F415L (TTT→CTT)	radD	putative DNA repair helicase RadD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/37);  total (34/37)

TACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGA  >  NC_000913/2281738‑2282010                                                                                                                                         |                                                                                                                                         tACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTg                                                                                                                                       <  1:238723/140‑1 (MQ=255) tACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGAACGCCGGCCGGACATTTGTTGAAAACTTTTGTCGGCGCCGGCAGGGATGGcttt                                                                                                                                       >  2:1920132/1‑139 (MQ=255)     agacagggCAAAAGTGAAAACGCTCCGGTACAGGTTTTCCGCCCTGCCAGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGa                                                                                                                                   <  2:1094288/134‑1 (MQ=255)       aaaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATg                                                                                                                                 >  1:1299289/1‑140 (MQ=255)       aaaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGACGTCGCTGTCAGGGATGGCTTGAAGATg                                                                                                                                 >  2:338601/1‑140 (MQ=255)        aaaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGACGTTGCCGTCAGGGATGGCTTGAAGactg                                                                                                                                >  1:916381/1‑137 (MQ=255)         aaGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATgac                                                                                                                               <  2:128606/140‑1 (MQ=255)              aaaaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACgg                                                                                                                          <  2:412085/140‑1 (MQ=255)                aaGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCTTCGGTGTCAGGGGTGGCTTGAAGATGACGACGGTc                                                                                                                        >  2:1065998/1‑140 (MQ=255)                  gTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCAt                                                                                                                      <  2:2429895/140‑1 (MQ=255)                   tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATc                                                                                                                     <  1:1949331/140‑1 (MQ=255)                   tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATc                                                                                                                     <  2:1299289/140‑1 (MQ=255)                   tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATc                                                                                                                     <  2:296627/140‑1 (MQ=255)                   tGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATc                                                                                                                     <  2:110631/140‑1 (MQ=255)                     aCAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATcgc                                                                                                                   >  2:1735086/1‑140 (MQ=255)                      cAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATcgcg                                                                                                                  <  1:1446116/140‑1 (MQ=255)                      cAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATcgcg                                                                                                                  <  2:474382/140‑1 (MQ=255)                      cAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTAGAAGATGACGACGGTcctgtct                                                                                                                  >  1:1094288/1‑134 (MQ=255)                         cGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAAc                                                                                                               <  1:2489671/140‑1 (MQ=255)                         cGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAAc                                                                                                               <  1:2255790/140‑1 (MQ=255)                         cGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAAc                                                                                                               <  1:1382047/140‑1 (MQ=255)                           ttCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACgg                                                                                                                          <  2:1527732/127‑1 (MQ=255)                           ttCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACgg                                                                                                                          >  1:1527732/1‑127 (MQ=255)                             ccGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGGCGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAAtg                                                                                                           <  2:1399754/140‑1 (MQ=255)                             ccGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAAtg                                                                                                           >  2:1261578/1‑140 (MQ=255)                                   cAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACtt                                                                                                     >  2:531784/1‑140 (MQ=255)                                         ttCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgttt                                                                                               <  1:338601/140‑1 (MQ=255)                                         ttCTGCCCTGCCTGCGGTTTTGACAACACTTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgttt                                                                                               <  1:860500/140‑1 (MQ=255)                                          tCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgtttc                                                                                              <  1:531784/140‑1 (MQ=255)                                           ctgccctgccTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgtttcc                                                                                             >  2:427925/1‑140 (MQ=255)                                           ctgccctgccTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgtttcc                                                                                             <  1:1640205/140‑1 (MQ=255)                                           ctgccctgccTGCGGATTTGCCGACGCCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACttccgtttcc                                                                                             <  1:1169694/140‑1 (MQ=255)                                              ccctgccTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTtccgtttccgtt                                                                                          >  2:2256610/1‑140 (MQ=255)                                                ctgcctgcGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGtttt                                                                                        >  2:1761608/1‑140 (MQ=255)                                                ctgcctgcGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGtttt                                                                                        >  2:1921079/1‑140 (MQ=255)                                                 tgcctgcGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTa                                                                                       >  2:280096/1‑140 (MQ=255)                                                       cGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAAtt                                                                                 <  2:2477268/140‑1 (MQ=255)                                                          cagtGCCAACACCTTTTGGGGGCAAACGATCGCCGACTGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGcc                                                                              <  1:891943/137‑1 (MQ=255)                                                            ttGCCAACACCTTTTGGGGGAAGACGACCGCCGACGGGAAATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCg                                                                            <  2:1118549/140‑1 (MQ=255)                                                            ttGCCAACACCTTTTGGGGGAACACGACCGCGGACCGGACATTGATTGGACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCg                                                                            <  2:986059/140‑1 (MQ=255)                                                             tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAGGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                           >  2:504133/1‑140 (MQ=255)                                                             tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGAGGACGGTCATCGGAAACTATGTGACTTCCGGTTCCATTTTAAAAATTGCCCGc                                                                           >  2:814928/1‑140 (MQ=255)                                                             tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCTAACAATGGGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                           >  2:1061865/1‑140 (MQ=255)                                                             tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                           >  2:2210696/1‑140 (MQ=255)                                                             tGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGc                                                                           >  1:1931498/1‑140 (MQ=255)                                                                      cTTTTGGGGGAAAACGACCGTCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAAcg                                                                  <  1:1762623/140‑1 (MQ=255)                                                                        tttGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAAcgcg                                                                <  2:2382404/140‑1 (MQ=255)                                                                        tttGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGAATTCGGTTACCGTTTTAAAAATTGCCCGCAATGTAAcctg                                                                >  2:891943/1‑137 (MQ=255)                                                                             gggAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGaaaa                                                           >  1:122063/1‑140 (MQ=255)                                                                                          ccgACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTgccgcc                                              >  2:2245084/1‑140 (MQ=255)                                                                                               gggACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCccgccg                                         <  1:1920132/140‑1 (MQ=255)                                                                                                    attgattgAACACTTTGGTCGTCGCTGTCAGGGATGGCTTAAAGATGACGAGGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAATTTGCCCGCAATGTAACGGGAAAAACGATTTTGCCGCCCGCCGCTGcc                                    <  2:1636737/140‑1 (MQ=255)                                                                                                     ttgattgaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcg                                   <  2:1931498/140‑1 (MQ=255)                                                                                                     ttgattgaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcg                                   <  1:2256610/140‑1 (MQ=255)                                                                                                      tgattgaACCCTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcgc                                  <  1:1622325/140‑1 (MQ=255)                                                                                                      tgattgaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCcgc                                  <  2:2009166/140‑1 (MQ=255)                                                                                                           gaACACTTTGGTCGTTGCTGTCCGGGATGGCTTGAAGGTGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGAAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                             <  1:828439/140‑1 (MQ=255)                                                                                                           gaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCGGCAATGTAACGCGGAAAACGATATTGCCGCGCGCCGCTGCCGCGAAtg                             >  2:1209826/1‑140 (MQ=255)                                                                                                           gaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTACCGCTGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                             >  2:828439/1‑140 (MQ=255)                                                                                                           gaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCTCTGCCGCGAAtg                             >  2:491161/1‑140 (MQ=255)                                                                                                           gaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                             <  1:1735086/140‑1 (MQ=255)                                                                                                           gaACACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAAAGATGTTTCCGCCCACCGCTGCCGCGAAtg                             >  1:806803/1‑140 (MQ=255)                                                                                                           gaACACTTTGGTCGTAGCTGTCGGGGATGTCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtg                             <  1:1065998/140‑1 (MQ=255)                                                                                                            aaCACTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAAtgt                            >  1:404566/1‑140 (MQ=255)                                                                                                              cccTTTGGTCGTCGCTGTCAGGGATGGCTAGAAGATGTCGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGAAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGa                          <  1:1209826/138‑1 (MQ=255)                                                                                                                cTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCTCTGCCGCGAATGTGaca                        <  1:491161/140‑1 (MQ=255)                                                                                                                cTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGaca                        <  1:1921079/140‑1 (MQ=255)                                                                                                                cTTTGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGaca                        <  1:1477178/140‑1 (MQ=255)                                                                                                                 tttGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGacac                       >  2:1481518/1‑140 (MQ=255)                                                                                                                  ttGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACAcc                      >  1:36978/1‑140 (MQ=255)                                                                                                                  ttGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACAcc                      >  2:154962/1‑140 (MQ=255)                                                                                                                   tGGTCGTCGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAGACGATATGGCCGCCCGGCGCTGCCGCGAATGTGACACCg                     >  2:771044/1‑140 (MQ=255)                                                                                                                     gtcgtcGCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTa                   <  1:154962/140‑1 (MQ=255)                                                                                                                     gtcgtcCCTGTCAGGGTTGGATGGAAGATGACGGCGATAATCGAGAAAGATGGGCCTTCCGTTCCGGTTTAAAAATTTCCCCGAAATGAAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTa                   <  2:1076793/140‑1 (MQ=255)                                                                                                                         tcgCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATAGCCGCCCCCCGCTGCCGCGAACGTGACACCGTACTgg               >  1:1058651/1‑140 (MQ=255)                                                                                                                           gCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt             >  1:2163177/1‑140 (MQ=255)                                                                                                                           gCTGTCAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt             >  1:578049/1‑140 (MQ=255)                                                                                                                           gCTGTAGGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGATTTCCTTTTCCGTTTTAAAAATTGCCCGCAATGTAGCGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGtt             <  2:2163177/140‑1 (MQ=255)                                                                                                                                cAGGGATGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATcc        >  2:579516/1‑140 (MQ=255)                                                                                                                                      tGGCTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGa  <  2:578049/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                         TACACCAAAAGGCAAAAGTGACAACGTTCCGGTACAGGTTTTCTGCCCTGCCTGCGGTTTTGCCAACACCTTTTGGGGGAAAACGACCGCCGACGGGACATTGATTGAACACTTTGGTCGTCGCTGTCAGGGATGGTTTGAAGATGACGACGGTCATCGCGAACAATGTGACTTCCGTTTCCGTTTTAAAAATTGCCCGCAATGTAACGCGGAAAACGATATTGCCGCCCGCCGCTGCCGCGAATGTGACACCGTACTGGTTGATCCGGACGA  >  NC_000913/2281738‑2282010

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑