Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,768,347	G→A	C213Y (TGT→TAT)	yfjP →	CP4‑57 prophage; putative GTP‑binding protein YfjP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,768,347	0	G	A	100.0%	313.6 / NA	93	C213Y (TGT→TAT)	yfjP	CP4‑57 prophage; putative GTP‑binding protein YfjP
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (52/41);  total (52/41)

TTCCACAGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT  >  NC_000913/2768210‑2768480                                                                                                                                            |                                                                                                                                      ttCCACAGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATc                                                                                                                                     >  2:1572587/1‑140 (MQ=255)  tCCACAGAGCAGAAACATAATAACAGCCGCAGAATTTGCATGCTGCATTAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCt                                                                                                                                    <  2:786220/140‑1 (MQ=255)  tCCACAGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCt                                                                                                                                    >  1:2406968/1‑140 (MQ=255)      caGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGTTGGCAGAGCGGATGATTAAGTATCTGCcg                                                                                                                                >  1:1066815/1‑140 (MQ=255)      caGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCcg                                                                                                                                >  1:1819008/1‑140 (MQ=255)       agagCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTGAGG‑‑GTGATGGCAGATCGGATGATTAAGTATCcgccgc                                                                                                                               >  1:855129/1‑140 (MQ=255)        gagCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTATGG‑‑GTGATGGCAGAGGGGATGAGTAAGTATCTGCcgcg                                                                                                                              >  1:648633/1‑140 (MQ=255)          gCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTg                                                                                                                            >  2:2092646/1‑140 (MQ=255)           cAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGa                                                                                                                           >  1:284393/1‑140 (MQ=255)           cAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGa                                                                                                                           >  2:1253488/1‑140 (MQ=255)           cAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAAG‑‑GTGATGGCAGAGCGGATGATCAAGTATCTGCCGCGTGa                                                                                                                           >  2:848579/1‑140 (MQ=255)           cAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTAGGGACTAAGG‑‑GTGATGACAGAGCGGGTGATTCAGTATCTGCCGCGTGa                                                                                                                           >  2:1076562/1‑140 (MQ=21)              aaaCAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGc                                                                                                                        >  2:504957/1‑140 (MQ=255)                 cAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcag                                                                                                                     <  1:1903769/140‑1 (MQ=255)                 cAAAATATCAGCCGCAAAATCTACCTGCTGCCTGACCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcag                                                                                                                     <  2:648633/140‑1 (MQ=255)                  aaaaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcagc                                                                                                                    <  2:2265814/140‑1 (MQ=255)                   aaaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGGTGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcag                                                                                                                     >  2:152003/1‑138 (MQ=255)                   aaaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGGTGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcag                                                                                                                     <  1:152003/138‑1 (MQ=255)                   aaaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcagca                                                                                                                   >  1:1822853/1‑140 (MQ=255)                    aaTATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCcagcag                                                                                                                  <  2:2180986/140‑1 (MQ=255)                     atatCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCagcagc                                                                                                                 >  2:451021/1‑140 (MQ=255)                      tatCAGCCGCAAAATCTGCCTGCTGTATGGGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGcc                                                                                                                <  1:734931/140‑1 (MQ=255)                      tatCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGcc                                                                                                                <  2:1965282/140‑1 (MQ=255)                       atCAGCCGCAAAATCTGGCTGCGGCATGAGCGATTACAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGccc                                                                                                               <  2:1095355/140‑1 (MQ=255)                        tCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAACGGCTGAATAAGTATCGGCCGGGTGAGGCCAGCAGCCCg                                                                                                              >  2:759278/1‑140 (MQ=18)                          aGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGgt                                                                                                            >  2:591328/1‑140 (MQ=255)                           gCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGgta                                                                                                           >  1:1374355/1‑140 (MQ=255)                            ccGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGgtag                                                                                                          >  1:1596042/1‑140 (MQ=255)                               cAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCTGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGtagtag                                                                                                       <  2:126453/140‑1 (MQ=255)                               cAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGtagtag                                                                                                       <  1:1307209/140‑1 (MQ=255)                                     ctgcctgcTGCATGAGCTATTCCAGCCCGTGGATCCGGTCTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTAc                                                                                                 <  2:938589/140‑1 (MQ=255)                                       gcctgcTGCATGAGCTATTCCAGCCCGTGCATCCGATGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTg                                                                                               <  1:1777278/140‑1 (MQ=255)                                             tgcATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACAt                                                                                         <  2:1374355/140‑1 (MQ=255)                                                  gAGCTATTCCAGCCCGTGCATCTGGTGTGTGGCGTTTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCtt                                                                                    <  2:1068450/140‑1 (MQ=255)                                                     cTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCg                                                                                 <  1:451021/140‑1 (MQ=255)                                                       aTTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               >  2:1875968/1‑140 (MQ=255)                                                       aTTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               <  2:1225719/140‑1 (MQ=255)                                                       aTTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               >  2:53350/1‑140 (MQ=255)                                                       aTTCCAGCCCGTGCATCCGGTGTGTGCCGTGCCGGATCGCCTGCAGTGTGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               <  2:844638/140‑1 (MQ=255)                                                        ttCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAACAGACCGGTAGTAGCGTTACTACAACATCCCTTTCGaac                                                                              >  2:892579/1‑140 (MQ=255)                                                           cAGCCCGTGCATCCGGTTTGTGGCGTGTCGGTTCGGCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCCGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaacaac                                                                           <  2:855129/140‑1 (MQ=255)                                                              cccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATccc                                                                                      <  1:2409065/126‑1 (MQ=255)                                                              cccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATccc                                                                                      >  2:2409065/1‑126 (MQ=255)                                                              cccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGt                                                                        >  2:28841/1‑140 (MQ=255)                                                              cccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGt                                                                        >  2:445042/1‑140 (MQ=255)                                                               ccGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTa                                                                       >  1:1490785/1‑140 (MQ=255)                                                                cGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GGGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAg                                                                      >  2:1404094/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCATTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTACCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                    >  1:1934145/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGGAGCCCGGTGGTAGCGCGACTGCAACGTCCCTTTCGAACAACGGTAGcc                                                                    >  2:669474/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                    >  2:1508644/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGGAGCGCTACTGCAACATCCCTTCCGAACAACGGTAGcc                                                                    >  1:1085970/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGACTAAGTATCTGCCGCGTGAGGCCAGGAGCCCGGTGGTAGCGCAACTGCAACGTCCCATTCGAACAACGGTAGcc                                                                    >  2:696519/1‑140 (MQ=255)                                                                  tGCATCCGGTGTGTGCCATGTCGGTTTGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGcc                                                                    >  2:2177916/1‑140 (MQ=255)                                                                   ggaTCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                   <  1:504957/138‑1 (MQ=255)                                                                   gCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGccc                                                                   <  2:2406968/140‑1 (MQ=255)                                                                     aTCCGTTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCTGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGAGCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCgg                                                                 <  2:1822853/140‑1 (MQ=255)                                                                        cGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAg                                                              <  1:1076562/140‑1 (MQ=255)                                                                          gtgtgtGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               >  1:1415442/1‑123 (MQ=255)                                                                          gtgtgtGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGGGTGTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGaa                                                                               <  2:1415442/123‑1 (MQ=255)                                                                            gtgtgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAgg                                                          <  1:611245/140‑1 (MQ=255)                                                                             tgtgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGc                                                         >  2:2235674/1‑140 (MQ=255)                                                                               tgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                       >  1:307926/1‑140 (MQ=255)                                                                               tgCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCAc                                                       >  2:1412907/1‑140 (MQ=255)                                                                                  cGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTg                                                    <  1:1253488/140‑1 (MQ=255)                                                                                    tgtCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                            >  2:1388752/1‑130 (MQ=255)                                                                                    tgtCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCa                                                            <  1:1388752/130‑1 (MQ=255)                                                                                    tgtCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGAc                                                  >  1:2052547/1‑140 (MQ=255)                                                                                     gtCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACg                                                 >  1:1939345/1‑140 (MQ=255)                                                                                                tGCAGTGGGGACTAAGT‑‑TTGATCGCAGAGCGTAGGTTTAGGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                      <  2:1085970/140‑1 (MQ=255)                                                                                                tGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGaa                                      <  1:1572587/140‑1 (MQ=255)                                                                                                     tGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGt                                 <  1:2235674/140‑1 (MQ=255)                                                                                                       gggACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                               <  1:1586820/140‑1 (MQ=255)                                                                                                       gggACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAg                               <  2:1819008/140‑1 (MQ=255)                                                                                                         gACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGt                             >  1:1663449/1‑140 (MQ=255)                                                                                                            tAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGcc                          >  2:1646440/1‑140 (MQ=255)                                                                                                            tAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGGAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGCGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGcc                          >  1:2049622/1‑140 (MQ=255)                                                                                                              aGG‑‑GTGATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCAt                        <  1:591328/140‑1 (MQ=255)                                                                                                               gg‑‑gTGATGGCAGAGCCGATGATAAAATATATGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATa                       <  1:759278/140‑1 (MQ=255)                                                                                                                     gATGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGACCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCg                   >  2:2465484/1‑140 (MQ=255)                                                                                                                      aTGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                  >  1:386241/1‑140 (MQ=255)                                                                                                                      aTGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                  >  1:2268670/1‑140 (MQ=255)                                                                                                                      aTGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGa                  >  2:1993448/1‑140 (MQ=255)                                                                                                                       tGGCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGAt                 <  2:258047/140‑1 (MQ=255)                                                                                                                         gCAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATAc               >  2:227742/1‑140 (MQ=255)                                                                                                                          cAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg              <  2:1236930/140‑1 (MQ=255)                                                                                                                          cAGAGCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACg              <  2:495392/140‑1 (MQ=255)                                                                                                                            gagCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt            >  1:1260346/1‑140 (MQ=255)                                                                                                                            gagCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt            >  2:2203449/1‑140 (MQ=255)                                                                                                                            gagCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGt            >  2:581673/1‑140 (MQ=255)                                                                                                                             agCGGATGATTAAGTATCTGCCTCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTa           >  1:1980679/1‑140 (MQ=255)                                                                                                                             agCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGCTTTCGGTAAAACCGAAGGTGCAATACTCGATACGGTa           >  1:973189/1‑140 (MQ=255)                                                                                                                              gCGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACAGTAGGTGCCGTACTCGATACGGTaa          >  1:1027323/1‑140 (MQ=255)                                                                                                                               cGGATGATTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAg         <  1:1993448/140‑1 (MQ=255)                                                                                                                                     atTAAGTATCTGCCGCGTGAGGCCATCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt   <  1:848579/140‑1 (MQ=255)                                                                                                                                     atTAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCtt   <  1:28841/140‑1 (MQ=255)                                                                                                                                      ttAAGTATCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCttt  <  2:307926/140‑1 (MQ=255)                                                                                                                                            |                                                                                                                                      TTCCACAGAGCAGAAACAAAATATCAGCCGCAAAATCTGCCTGCTGCATGAGCTATTCCAGCCCGTGCATCCGGTGTGTGCCGTGTCGGTTCGCCTGCAGTGGGGACTAAGG‑‑GTGATGGCAGAGCGGATGATTAAGTGTCTGCCGCGTGAGGCCAGCAGCCCGGTAGTAGCGCTACTGCAACATCCCTTTCGAACAACGGTAGCCCGGGAGCAGGCACGTGACGATTTCGGTGAAACCGTAGGTGCCATACTCGATACGGTAAGCACCTTT  >  NC_000913/2768210‑2768480

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑