BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,968,718	T→C	D87G (GAC→GGC)	rppH ←	RNA pyrophosphohydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,968,718	0	T	C	98.5%	463.7 / NA	136	D87G (GAC→GGC)	rppH	RNA pyrophosphohydrolase
Reads supporting (aligned to +/- strand): ref base T (2/0); new base C (66/67); total (68/68)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 145 total aligned reads displayed.

CCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTC  >  NC_000913/2968581‑2968850

ccGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGccc                                                                                                                                    >  2:53564/1‑140 (MQ=255)

 cGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGcccc                                                                                                                                   >  2:132873/1‑140 (MQ=255)

 cGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGcccc                                                                                                                                   >  1:1820018/1‑140 (MQ=255)

  ggATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCa                                                                                                                                  <  1:2070446/140‑1 (MQ=255)

  ggATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATATTTGTTTGTGGGCGCTACAAACCGGCTTCGTGCCCCa                                                                                                                                  >  1:926410/1‑140 (MQ=255)

   gATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCaa                                                                                                                                 <  1:523690/140‑1 (MQ=255)

     tACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACg                                                                                                                               <  1:132873/140‑1 (MQ=255)

     tACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACg                                                                                                                               >  1:2411803/1‑140 (MQ=255)

          gTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCa                                                                                                                          <  2:1615385/140‑1 (MQ=255)

             aCTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAAc                                                                                                                       >  1:2322538/1‑140 (MQ=255)

                 aCCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGttt                                                                                                                   <  1:1713578/140‑1 (MQ=255)

                 aCCCATCGCCAGCCGCCAAACTTTGTTGTCCTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGttt                                                                                                                   <  1:774991/140‑1 (MQ=255)

                  cccATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTc                                                                                                                  >  1:290495/1‑140 (MQ=255)

                   ccATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCg                                                                                                                 <  1:1947106/140‑1 (MQ=255)

                      tCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTa                                                                                                              >  2:90501/1‑140 (MQ=255)

                      tCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTGGGCCGATAAAAAGCTGCATCGTGCCCCAACCAACCAAACATTTCGGTa                                                                                                              >  2:1200185/1‑140 (MQ=255)

                       cGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAGCCGGCTTCGTGTCCGAACGCAACAAACGTTTCGGTaa                                                                                                             >  1:998805/1‑140 (MQ=255)

                       cGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCAAACGCAGCAAACGTTTCGGTaa                                                                                                             >  1:889414/1‑140 (MQ=255)

                       cGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGGaa                                                                                                             >  2:1987186/1‑140 (MQ=255)

                         ccAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAAtt                                                                                                           >  2:2023886/1‑140 (MQ=255)

                          cAGCCGTCAAACTCTCGTGTACCGCCGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAAttt                                                                                                          <  1:779477/140‑1 (MQ=255)

                             ccGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTa                                                                                                       >  1:1668494/1‑140 (MQ=255)

                                         ggTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTACCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGtt                                                                                           <  2:202492/140‑1 (MQ=255)

                                          gtgtACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTa                                                                                          >  2:1571427/1‑140 (MQ=255)

                                          gtgtACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTa                                                                                          <  2:256504/140‑1 (MQ=255)

                                          gtgtACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGACCGATACAAACCGGCTTCGTGCCCCAACGCACCAAAAGTTGCGTTAATTTTTAGCGCAACCAGTTa                                                                                          >  1:788462/1‑140 (MQ=255)

                                             tACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgc                                                                                       <  2:252123/140‑1 (MQ=255)

                                             tACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgc                                                                                       >  1:1331266/1‑140 (MQ=255)

                                              aCTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCTCCACACGGTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                      >  2:978923/1‑140 (MQ=255)

                                              aCTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                      <  1:2144683/140‑1 (MQ=255)

                                              aCTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                      >  2:2174303/1‑140 (MQ=255)

                                              aCTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTAcgcg                                                                                      <  1:1534116/140‑1 (MQ=255)

                                               ctgctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGt                                                                                     >  1:440729/1‑140 (MQ=255)

                                                tgctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGtt                                                                                    >  2:2112368/1‑140 (MQ=255)

                                                  ctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGa                                                                                  >  1:178752/1‑140 (MQ=255)

                                                  ctgGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATGTGCAGCGCAACCAGTTACGCGTTGa                                                                                  >  1:1090945/1‑140 (MQ=255)

                                                    ggTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAAc                                                                                                <  1:301409/124‑1 (MQ=255)

                                                    ggTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAAc                                                                                                >  2:301409/1‑124 (MQ=255)

                                                    ggTTTGCATATTGATTTATGCATCGCCGCTCTCCACCTGCAAGAGAAAACATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAg                                                                                <  2:718667/140‑1 (MQ=255)

                                                     gTTTGCATATTGATTTCAGCATTGCCGCTCAACAGATGCTAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGc                                                                               <  2:788462/140‑1 (MQ=255)

                                                      tttGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCa                                                                              <  2:2303303/140‑1 (MQ=255)

                                                       ttGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCaa                                                                             >  2:2212608/1‑140 (MQ=255)

                                                        tGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAg                                                                            <  1:1428730/140‑1 (MQ=255)

                                                          cATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGa                                                                          >  1:2478894/1‑140 (MQ=255)

                                                           atatTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGAt                                                                         <  1:1831505/140‑1 (MQ=255)

                                                               tGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCa                                                                              <  1:2200029/131‑1 (MQ=255)

                                                               tGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCa                                                                              >  2:2200029/1‑131 (MQ=255)

                                                               tGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGa                                                                     >  2:1418673/1‑140 (MQ=255)

                                                               tGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTCGTAGCGCCACCAGTTACGCGTTGAAGCAAGGATTCGa                                                                     >  1:1071092/1‑140 (MQ=255)

                                                                gATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACCCACCAACCGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGaa                                                                    >  2:463882/1‑140 (MQ=255)

                                                                 aTTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAAc                                                                   <  1:2098771/140‑1 (MQ=255)

                                                                    tCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTGGCGGAACGAGTTACGCATTGAGGCAAGGATTCGAActgt                                                                >  2:747191/1‑137 (MQ=255)

                                                                    tCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGc                                                                               <  1:2017885/125‑1 (MQ=255)

                                                                     cTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCt                                                               >  2:1301737/1‑140 (MQ=255)

                                                                       gCATCGCCGCTCACCCGCTGCACGACAAACCATTTTTATTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCttt                                                             <  1:742234/140‑1 (MQ=255)

                                                                        cATCGCCGCTCACCATCTGCAATAGTAACCATTTTTGCTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTg                                                            <  1:714138/140‑1 (MQ=255)

                                                                           cgccgcTCACCAGCTGGCAGAGCAACCATTTCTCTTTTTGGCCGATACGAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCgg                                                         <  2:1090945/140‑1 (MQ=255)

                                                                            gccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGc                                                        <  1:1920993/140‑1 (MQ=255)

                                                                             ccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCt                                                       <  1:1687578/140‑1 (MQ=255)

                                                                             ccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCt                                                       <  1:571638/140‑1 (MQ=255)

                                                                             ccgcTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCt                                                       <  1:2212608/140‑1 (MQ=255)

                                                                                cTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCTCAACCAGTCACGCGTTGAAGCAAGGATTCGTACGTCTTTGCGGCTTaa                                                    >  2:943952/1‑140 (MQ=255)

                                                                                     cAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAAt                                                   <  2:2135996/136‑1 (MQ=255)

                                                                                        cTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActt                                            >  1:13742/1‑140 (MQ=255)

                                                                                        cTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActt                                            >  2:1737390/1‑140 (MQ=255)

                                                                                        cTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGCGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActt                                            >  2:114411/1‑140 (MQ=255)

                                                                                            aaGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACttcttc                                        <  2:1492298/140‑1 (MQ=255)

                                                                                             agagAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCa                                       >  2:2346499/1‑140 (MQ=255)

                                                                                             agagAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCa                                       >  2:1484810/1‑140 (MQ=255)

                                                                                             agagAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAACGATTCGACCGTCTTGGCGGCTGAATCCTACTTCTTCa                                       >  1:972851/1‑140 (MQ=255)

                                                                                               ggaAACCATTTTTGTTTATGGCGGATACAAATCGGCTTCTTGCCCCAAAGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCaaa                                     <  2:889414/139‑1 (MQ=255)

                                                                                               agaAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCaaa                                     <  1:1484810/140‑1 (MQ=255)

                                                                                               agaAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCaaa                                     <  2:1932227/140‑1 (MQ=255)

                                                                                               agaAACCATTTTTGTTTTTGGCCGATACAAACCGCCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGATGAAGCAATGATACGACCGTCTTTGAGGCTAAATCCTACTTCTTCaaa                                     >  2:678322/1‑140 (MQ=255)

                                                                                                 aaaCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACa                                   <  2:178752/140‑1 (MQ=255)

                                                                                                 aaaCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACa                                   >  2:121440/1‑140 (MQ=255)

                                                                                                  aaCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActtct                                          <  2:215621/132‑1 (MQ=255)

                                                                                                  aaCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTActtct                                          >  1:215621/1‑132 (MQ=255)

                                                                                                   aCCATTTTTGTTTTTTGCCGATGCAGACCAGCTTCGTTCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAAt                                 <  1:1200185/140‑1 (MQ=255)

                                                                                                   aCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAAt                                 >  1:609000/1‑140 (MQ=255)

                                                                                                   aCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAAt                                 >  1:1244361/1‑140 (MQ=255)

                                                                                                   aCCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCAAACGTCTGTGCGGCATAATCCGACTTCTTCAAACAAt                                 >  1:718368/1‑140 (MQ=255)

                                                                                                       tttttgtttttgGCCTATACAATCCTGCTTCGTGCCCAAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCAc                             <  1:943952/140‑1 (MQ=255)

                                                                                                       tttttgtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCAc                             <  2:290495/140‑1 (MQ=255)

                                                                                                          ttgtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGt                          <  1:90501/140‑1 (MQ=255)

                                                                                                          ttgtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCAAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGt                          <  2:516121/140‑1 (MQ=255)

                                                                                                          ttgtttttgGCCGATACAAACCGGCATCGAGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGt                          <  1:745741/140‑1 (MQ=255)

                                                                                                            gtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTAc                        <  1:1748422/140‑1 (MQ=255)

                                                                                                            gtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTAc                        >  2:1687777/1‑140 (MQ=255)

                                                                                                            gtttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTAc                        <  1:114411/140‑1 (MQ=255)

                                                                                                              ttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCa                              <  2:114731/132‑1 (MQ=255)

                                                                                                              ttttgGCCGATACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCa                              >  1:114731/1‑132 (MQ=255)

                                                                                                                       aTACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGctc             >  2:2332793/1‑140 (MQ=255)

                                                                                                                        tACAAACCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGctct            <  2:1820018/140‑1 (MQ=255)

                                                                                                                             cccGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGa       <  2:1793314/139‑1 (MQ=255)

                                                                                                                             aCCGGCTTCGTGCCCGAACGCACCACACGGTTCGGTAGTTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGa       <  1:1027827/140‑1 (MQ=255)

                                                                                                                             aCCGGCTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGa       <  1:151213/140‑1 (MQ=255)

                                                                                                                                  cTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCGAACAATTAACGCTACATCGCCCGCTCTGCGGATtctc  >  1:789299/1‑140 (MQ=255)

                                                                                                                                  cTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATtctc  <  1:1418673/140‑1 (MQ=255)

                                                                                                                                  cTTCGTGCCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATtctc  >  1:571390/1‑140 (MQ=255)

CCGGATACCAGTAACTTACCCATCGCCAGCCGTCAAACTCTGGTGTACTGCTGGTTTGCATATTGATTTCTGCATCGCCGCTCACCAGCTGCAAGAGAAACCATTTTTGTTTTTGGCCGATACAAACCGGCTTCGTGTCCCAACGCACCAAACGTTTCGGTAATTTGTAGCGCAACCAGTTACGCGTTGAAGCAAGGATTCGAACGTCTTTGCGGCTTAATCCTACTTCTTCAAACAATTCACGGTACATCGCCTGCTCTGCGGATTCTC  >  NC_000913/2968581‑2968850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`