BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,119,973	G→A	S436F (TCC→TTC)	glcA ←	glycolate/lactate:H(+) symporter GlcA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,119,973	0	G	A	100.0%	415.7 / NA	120	S436F (TCC→TTC)	glcA	glycolate/lactate:H(+) symporter GlcA
Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (56/64); total (56/64)

Only 100 of 123 total aligned reads displayed.

TGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGAAGA  >  NC_000913/3119836‑3120105

tGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAAc                                                                                                                                    >  2:1931928/1‑140 (MQ=255)

 ggtgaaTTGCAGCAAAAAAAACAGGGTGTTAGAGG‑GGTTTCCGGACCCGGTAAGGAATACGCCCAGCCAGCCGAGAACCGGTGAGAAGAACGGGAACATCCCGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACa                                                                                                                                   <  2:1980766/135‑1 (MQ=255)

     gtttGCAGTAACCAAAAGGGGGCGTAGGGGGGGGTGCCG‑GAGCGGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGcc                                                                                                                               <  2:2318302/138‑1 (MQ=255)

     gttggaaGTAAACAAAAAGGGGCTTAAGAGGGGGTGCCG‑GAGCGGGTAAGAAATCCGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGcc                                                                                                                               <  2:2173731/134‑1 (MQ=255)

          cAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAAt                                                                                                                          >  1:1850699/1‑140 (MQ=255)

                aCCAAACAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTgg                                                                                                                    >  1:2035798/1‑140 (MQ=255)

                    aaCAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGGCAGCGTGGTGAACATGCCAGAATAGTTGGTGAc                                                                                                                >  2:1907812/1‑140 (MQ=255)

                    aaCAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGAc                                                                                                                >  1:339261/1‑140 (MQ=255)

                      cAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGa                                                                                                              >  1:187526/1‑140 (MQ=255)

                      cAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGa                                                                                                              <  1:1976315/140‑1 (MQ=255)

                         ggCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAgg                                                                                                           >  2:60925/1‑140 (MQ=255)

                         ggCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAgg                                                                                                           >  1:100769/1‑140 (MQ=255)

                         ggCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAgg                                                                                                           >  2:1251748/1‑140 (MQ=255)

                         ggCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAgg                                                                                                           >  1:1835190/1‑140 (MQ=255)

                                   gaggTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAg                                                                                                 >  2:231532/1‑140 (MQ=255)

                                   gaggTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAg                                                                                                 >  2:2203989/1‑140 (MQ=255)

                                     ggTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCa                                                                                               >  1:2179763/1‑140 (MQ=255)

                                      gtgtCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACTTGCCAGAATAGTTGGGGACGACGGCGAACGCCAGCAc                                                                                              >  1:1178547/1‑140 (MQ=255)

                                      gtgtCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCAc                                                                                              >  1:65112/1‑140 (MQ=255)

                                       tgtCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCAcc                                                                                             >  1:1240240/1‑140 (MQ=255)

                                        gtCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCa                                                                                            >  2:352940/1‑140 (MQ=255)

                                        gtCC‑GAGCCGGTAAGGAATACGCCCAGCCAACCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTAACGAAGGCGAACGCCAGCACCa                                                                                            >  1:653953/1‑140 (MQ=255)

                                            gaggCCGGTAAGAAATTTCCCCAGCCAGCCGAGAAGCGGTGAGAAGAAGGGAAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGc                                                                                         <  2:1098102/137‑1 (MQ=255)

                                              aGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCa                                                                                       <  1:352940/140‑1 (MQ=255)

                                                   gTAAGGAATCCGCCCAGCCAGCCGAGAAACGGTGAGAAGACCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                                                                  <  1:539284/140‑1 (MQ=255)

                                                   gTAAGGAATACGCCCAGCCGGCCGAGAAACGGGGAGAAGACCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATAGa                                                                                  <  1:1240297/140‑1 (MQ=255)

                                                   gTAAGGAACCCGCCCCGCCAGCCGAAAACCCTTTATAAGACCGGGAACTCCCCCCCTTTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGTACGGCAGTACCATGCCAATCgg                                                                                  <  2:1178547/140‑2 (MQ=255)

                                                   gTAAGGAACCCGCCCAGCCAGCCGAGAAACGGTGAAAAAAACGGAAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGa                                                                                  <  2:2113593/140‑1 (MQ=255)

                                                    tAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGAc                                                                                 >  2:2048528/1‑140 (MQ=255)

                                                    tAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGAc                                                                                 >  2:2442400/1‑140 (MQ=255)

                                                    tAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGAc                                                                                 >  2:309066/1‑140 (MQ=255)

                                                         aaTACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCAAACGC‑‑GGACCATGACAATCGACAGTATCg                                                                          >  1:748896/1‑140 (MQ=255)

                                                            ccccccAGCCAGCCGAGAACCGGTGAGAAGAACGGAAACACCCCGCCTGTCCCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCgg                                                                         <  2:1970562/137‑1 (MQ=255)

                                                            ccGCCCACCCACCCGAGAAAGGGTGAGAAGAACGGGAACATCCCGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCgg                                                                         <  2:1764136/139‑1 (MQ=255)

                                                             ggCCAAGCCCGCCAGGAAACGGTGAAAAGAACGGGAAGCTCACGCCTGGACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCCCCATGCCAATCGACAGTATCGGc                                                                        <  1:1624987/139‑1 (MQ=255)

                                                             cGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGc                                                                        >  2:398472/1‑140 (MQ=255)

                                                             cGCCCAGCAAGCAGAAACACGGTGAGAAGAACGCAGACAGCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGc                                                                        <  2:1177817/140‑1 (MQ=255)

                                                                 cagccagcGGAGAACGGGTGAGCAGAACGTGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACt                                                                    <  1:822991/140‑1 (MQ=255)

                                                                 cagccagcGGAAAAACGGTGAGAAGAAGGGGAACAACCCGTCTGTGCCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACt                                                                    <  1:629628/140‑1 (MQ=255)

                                                                  agccagccGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACtt                                                                   >  2:165852/1‑140 (MQ=255)

                                                                  agccagccGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACtt                                                                   >  2:187673/1‑140 (MQ=255)

                                                                  agccagccGAGAAACGGCGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACt                                                                    <  1:435937/139‑1 (MQ=255)

                                                                  agccagccGAGAAACGGCGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACt                                                                    >  2:435937/1‑139 (MQ=255)

                                                                   gccagccGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCCCCATGCGAATCGATAGTATCGGCCACTTc                                                                  >  1:1145154/1‑140 (MQ=255)

                                                                                    gagaAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTc                                                 >  1:25508/1‑140 (MQ=255)

                                                                                       aagaaCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGc                                              <  1:1907812/140‑1 (MQ=255)

                                                                                       aagaaCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGc                                              <  1:2442400/140‑1 (MQ=255)

                                                                                        agaaCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCa                                             <  1:475461/140‑1 (MQ=255)

                                                                                          aaCGGGAACATCCCGCCTGTCCCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCaaa                                           <  2:234607/140‑1 (MQ=255)

                                                                                          aaCGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCaaa                                           >  1:265269/1‑140 (MQ=255)

                                                                                            cGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGa                                         <  1:1908937/140‑1 (MQ=255)

                                                                                               gAACATCCCGCCTGTACCTGCCTGTACCAGCGCCAGCGTCGTGAACATACCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGc                                      <  2:653953/140‑1 (MQ=255)

                                                                                                     ccagCCTTTCCCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATAc                                <  2:2179763/137‑1 (MQ=255)

                                                                                                     cACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATAc                                <  1:445013/140‑1 (MQ=255)

                                                                                                     cACCCCTGCCCCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATAc                                <  2:339261/140‑1 (MQ=255)

                                                                                                      aCGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATAcc                               <  1:1693863/140‑1 (MQ=255)

                                                                                                       cGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCt                              >  1:360100/1‑140 (MQ=255)

                                                                                                       cGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCAATTCAAGCAAATTAGCGCTTCGCCAAAGACGCCAATACCt                              >  2:808308/1‑140 (MQ=255)

                                                                                                            ttcccTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGCTTCGGCAAAGACGCCAATACCTTTCtt                         <  2:1412165/137‑1 (MQ=255)

                                                                                                            gtcccTGCCAGTACCAGCGCCACCGTGGTGACCATACCATAATAGTTGTTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCtt                         <  2:824826/137‑1 (MQ=255)

                                                                                                            gTACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCtt                         <  1:231532/140‑1 (MQ=255)

                                                                                                             tACCTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTg                        >  1:606768/1‑140 (MQ=255)

                                                                                                              cccTGCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGa                       <  2:617072/139‑1 (MQ=255)

                                                                                                                  gCCAGTACCAGCGCCGGCGTGGAGAACATACCAGAATTGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATccc                   <  1:855542/140‑1 (MQ=255)

                                                                                                                  gCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCTAATCGACAGTATCGGCCACTTCAAGCTAATTGGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATccc                   <  1:60925/140‑1 (MQ=255)

                                                                                                                  gCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATccc                   >  2:397438/1‑140 (MQ=255)

                                                                                                                  gCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATccc                   <  2:265269/140‑1 (MQ=255)

                                                                                                                  gCCAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATccc                   <  1:1890113/140‑1 (MQ=255)

                                                                                                                    cAGTACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCca                 >  1:2034495/1‑140 (MQ=255)

                                                                                                                      gTACCAGCGCCAGCGTGTTGAAGATGCCAGATTAGTTGGTGACGAAGGCGAACGC‑‑GCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACAcc             <  2:748896/140‑1 (MQ=255)

                                                                                                                       tACCAGCGTCAGCGTGGTGAACGTGCCAGATTACTGGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCacac              <  2:1145154/140‑1 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCtt                         >  2:1861613/1‑129 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCtt                         <  1:1861613/129‑1 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCacac              <  1:601242/140‑1 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCacac              >  2:1870659/1‑140 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGATAATTAGCGTTTGGGCGAAGACGCCCATACATTTCTTGATCCCCacac              >  2:753608/1‑140 (MQ=255)

                                                                                                                       tACCAGCGCCAGCGTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCAAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCacac              >  2:1962562/1‑140 (MQ=255)

                                                                                                                         ccagcgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCg            <  1:398472/140‑1 (MQ=255)

                                                                                                                         ccagcgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCg            <  2:1925712/140‑1 (MQ=255)

                                                                                                                         ccagcgccagcgTGGGGAACATACCAGAATAGTTGGTCACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCg            <  1:963757/140‑1 (MQ=255)

                                                                                                                             cgccagcgTGGTGAACATGCCATAATAGTCGGTGACGTAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGa        <  1:808308/140‑1 (MQ=255)

                                                                                                                             cgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGa        >  1:1841361/1‑140 (MQ=255)

                                                                                                                             cgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGa        >  1:1664244/1‑140 (MQ=255)

                                                                                                                             cgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGa        <  1:1941804/140‑1 (MQ=255)

                                                                                                                             cgccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGa        >  2:2137473/1‑140 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       <  2:1866321/140‑1 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       <  2:360100/140‑1 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       <  1:2489136/140‑1 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       <  1:1732281/140‑1 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       >  2:414030/1‑140 (MQ=255)

                                                                                                                              gccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGAt       <  1:67008/140‑1 (MQ=255)

                                                                                                                               ccagcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATg      <  2:576466/140‑1 (MQ=255)

                                                                                                                                 agcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCg            >  1:2251690/1‑132 (MQ=255)

                                                                                                                                 agcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGaa    >  1:379160/1‑140 (MQ=255)

                                                                                                                                 agcgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGaa    >  2:1330571/1‑140 (MQ=255)

                                                                                                                                   cgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGAAGa  <  1:2137473/140‑1 (MQ=255)

                                                                                                                                   cgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGAAGa  <  1:397438/140‑1 (MQ=255)

                                                                                                                                   cgTGGTGAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGAAGa  <  1:2203989/140‑1 (MQ=255)

                                                                                                                                    gtggtgAACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATg      <  1:1824925/135‑1 (MQ=255)

                                                                                                                                    gcggtgaACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCCCACCGAGGATg      >  2:1824925/3‑135 (MQ=255)

TGGTCGATTGCAGTGAACCAAACAGGGCGTTAGAGGAGGTGTCC‑GAGCCGGTAAGGAATACGCCCAGCCAGCCGAGAAACGGTGAGAAGAACGGGAACATCACGCCTGTACCTGCCAGTACCAGCGCCAGCGTGGTGGACATGCCAGAATAGTTGGTGACGAAGGCGAACGCCAGCACCATGCCAATCGACAGTATCGGCCACTTCAAGCTAATTAGCGTTTCGGCAAAGACGCCAATACCTTTCTTGATCCCCACACCGAGGATGAAGA  >  NC_000913/3119836‑3120105

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`