Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,180,279	T→C	V179A (GTA→GCA)	ygiB →	DUF1190 domain‑containing protein YgiB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,180,279	0	T	C	100.0%	313.1 / NA	91	V179A (GTA→GCA)	ygiB	DUF1190 domain‑containing protein YgiB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/47);  total (44/47)

TCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGG  >  NC_000913/3180142‑3180407                                                                                                                                          |                                                                                                                                 tCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCAc                                                                                                                                >  1:5800/1‑140 (MQ=255)   tGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCg                                                                                                                              <  2:1361923/140‑1 (MQ=255)      tGGGCGGCGGCGCGGTATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAg                                                                                                                           <  1:848487/140‑1 (MQ=255)            gcggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCa                                                                                                                     >  2:36297/1‑140 (MQ=255)              ggcgCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAAt                                                                                                                   <  1:1880217/140‑1 (MQ=255)                           cacaGCAGCCGCTGTTCTCCTCCAAAAACTCAGCCAGACCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCg                                                                                                      <  1:685250/140‑1 (MQ=255)                              agcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCg                                                                                                   <  2:1828065/140‑1 (MQ=255)                               gcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGc                                                                                                                >  1:2185470/1‑126 (MQ=255)                               gcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGc                                                                                                                <  2:2185470/126‑1 (MQ=255)                               gcagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGa                                                                                                  >  1:1556812/1‑140 (MQ=255)                                cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGTCCGCACCAAAGGCGACAATGGCACCAAAACCGGCGac                                                                                                 >  1:795869/1‑140 (MQ=255)                                cagcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGac                                                                                                 <  2:2382459/140‑1 (MQ=255)                                  gcCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGacca                                                                                               <  2:1556812/140‑1 (MQ=255)                                   ccGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCCCGGAAGACGGCAGTGGCACCAAAACCGGCGaccac                                                                                              >  2:1102814/1‑140 (MQ=255)                                     gCTGTTCTCCTCGAAAAACCCATCCAGTCCGCCTTCCGGGAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGAccacca                                                                                            <  2:739956/140‑1 (MQ=255)                                      cTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACcaccac                                                                                           <  1:140578/140‑1 (MQ=255)                                           ctcctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCGCCACTACCg                                                                                      >  1:1781618/1‑140 (MQ=255)                                              ctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTa                                                                                   >  2:2258752/1‑140 (MQ=255)                                              ctcGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTa                                                                                   >  2:2391162/1‑140 (MQ=255)                                                   aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCCCCAAAACCGGCGACCACCACTACCGTTACCCGt                                                                              >  2:1103726/1‑140 (MQ=255)                                                   aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGt                                                                              >  1:2097830/1‑140 (MQ=255)                                                   aaaaCCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAAAGGCACCAAAACTGGAGACCACGACTACCGTTACCCGt                                                                              >  1:1149678/1‑140 (MQ=255)                                                        ccagccagTCCGGCTTACGGTAAATATACCGACGCGACGGGGAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCGACACCGGCGACCGCCACTCCCGTTACCCGTGGCgg                                                                         >  2:1098931/1‑140 (MQ=255)                                                           gccagTCCGGCTTACCGTAAATGTAGCGACGCGACGGTTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGttt                                                                      <  1:1098931/140‑1 (MQ=255)                                                            ccagTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGtttt                                                                     >  1:2393325/1‑140 (MQ=255)                                                               gTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGt                                                                  <  2:5800/140‑1 (MQ=255)                                                                tCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGCTTTggcg                                                                 >  2:39358/1‑138 (MQ=255)                                                                  cGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGaa                                                               >  1:2128921/1‑140 (MQ=255)                                                                      ttACGGTAAATATCCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCCCCATTACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                           <  2:526538/140‑1 (MQ=255)                                                                      ttACGGTAAATATACCGACGCGGCGGGTAAATACTATGGAGCAGCCCATCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                           <  2:721557/140‑1 (MQ=255)                                                                      ttACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTg                                                           <  1:49411/140‑1 (MQ=255)                                                                           gTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGcc                                                      <  2:2097830/140‑1 (MQ=255)                                                                           gTAAATATACCGACGCGACGGGTAAAAACTACGGCCCAGCCCAGCCAGTCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGcc                                                      <  2:656886/140‑1 (MQ=255)                                                                           gTAAATATACCGACGCAACGGGCAAAAACTATGTCTCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGcc                                                      <  2:1155615/140‑1 (MQ=255)                                                                                tataCCGACGCGACGGGTAAAATCTTTGGCGCAGCCCGGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 <  1:1203817/140‑1 (MQ=255)                                                                                tataCCGACGCGACGGGTAAAACCTATGGCGAAGCGCAGCCAGGCCGCGCCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 <  2:894026/140‑1 (MQ=255)                                                                                tataCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 <  1:1622581/140‑1 (MQ=255)                                                                                   aCCGACGCGACGGGTAACAACTTTGGCGCAGCCCATCCACGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAg                                              <  2:698660/140‑1 (MQ=255)                                                                                   aCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTTGGGAATCTGTTTGcaaaaaaaa                                              >  2:1622581/1‑139 (MQ=255)                                                                                   aCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 <  2:515360/137‑1 (MQ=255)                                                                                   aCCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 >  1:515360/1‑137 (MQ=255)                                                                                    ccGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGc                                             >  2:32388/1‑140 (MQ=255)                                                                                    ccGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGGTTTGGGGAATCTGTTTCccaacaaaac                                             >  2:345820/1‑138 (MQ=255)                                                                                     cgacgcgacgGGTAAAATCTATAGCGCAGCCGAGCTAGGGCGCACCATTACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            <  2:795869/140‑1 (MQ=255)                                                                                     cgacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCGCCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTTCCAAACAAAGCa                                            <  2:1781618/140‑1 (MQ=255)                                                                                     cgacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            <  2:2128921/140‑1 (MQ=255)                                                                                      gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCATTACCGTTAACCGGGGCAGTTTTGGTGAATCTGTTGCCCAACAAAGCAc                                           >  2:733146/1‑140 (MQ=255)                                                                                      gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAc                                           >  1:133789/1‑140 (MQ=255)                                                                                      gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAc                                           >  2:2174873/1‑140 (MQ=255)                                                                                      gacgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCAc                                           >  1:2137361/1‑140 (MQ=255)                                                                                       acgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACt                                          >  1:430057/1‑140 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTGCCGTTACCAGTGCCGGCTTTTGTGGATCTGGGTCCAAACAAAACACTa                                         >  2:1134347/1‑140 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaagca                                                 <  2:1589285/132‑1 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCcaaaca                                                 >  1:1589285/1‑132 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            <  2:1901660/137‑1 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            <  2:1628165/137‑1 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            >  1:1901660/1‑137 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCa                                            >  1:1628165/1‑137 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTa                                         >  1:2004120/1‑140 (MQ=255)                                                                                        cgcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGGGGCGGTTATGGAGAGTCTGTTGCCAAACAAAGCACTa                                         >  1:698660/1‑140 (MQ=255)                                                                                         gcgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGGTTTGGGGAATCTTTTGCCAAACAAAACACTAt                                        >  2:2370044/1‑140 (MQ=255)                                                                                          cgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTTGTGAAACTGTTGGCCAACAAAACACCATg                                       >  2:2414489/1‑140 (MQ=255)                                                                                          cgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                       >  1:2130983/1‑140 (MQ=255)                                                                                          cgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTATGGTGAATCTGTTTCCAAACAAAGCACTATg                                       >  2:887141/1‑140 (MQ=255)                                                                                          cgacgGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGACACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATg                                       <  1:1627070/140‑1 (MQ=255)                                                                                                         aTGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAAcc                        <  1:36297/140‑1 (MQ=255)                                                                                                          tGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCaaacc                       >  1:1472058/1‑137 (MQ=255)                                                                                                          tGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCg                       >  1:1705478/1‑140 (MQ=255)                                                                                                          tGGCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTGTTGGGGAACCTGATGCAAAACAAAACACCAGTCAGAGGAGTGCCAccc                       >  2:722097/1‑139 (MQ=255)                                                                                                          gggCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCg                       <  1:486678/139‑1 (MQ=255)                                                                                                           ggCGCAGCCCAGCCAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTATGGGGAAATTGTAGCCAAACAAAGCGACATGCCGCGTAGGTCAACCgg                      >  2:677695/1‑140 (MQ=255)                                                                                                               cagcccagccAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTAcc                  <  2:2003910/140‑1 (MQ=255)                                                                                                                agcccagccAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTCCCACACAAAACACTATACAGCATAGTGCAACCGGGAccc                 >  2:1024170/1‑139 (MQ=255)                                                                                                                  cccagccAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCCAACAAAACACTATGCAGCGGAGTGCAACCGGGACctct               >  2:1351916/1‑140 (MQ=255)                                                                                                                  cccagccAGGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACctct               >  1:546422/1‑140 (MQ=255)                                                                                                                   ccagccagGCCGCACCATGACCTCAACGAGGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtctt              <  1:1134347/140‑1 (MQ=255)                                                                                                                     agccagGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCtcttct            <  1:39358/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACGGCACCGTAGACGGTAATGGCAACAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:1024629/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACGGCACCGCAGCCGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:733146/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGTAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:1102814/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGTAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  2:2393325/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  2:546422/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:1351916/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:300294/140‑1 (MQ=255)                                                                                                                         agGCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGtt        <  1:2258752/140‑1 (MQ=255)                                                                                                                           gCCGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa      <  2:430057/140‑1 (MQ=255)                                                                                                                            ccGCACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGGTTTGGTGAATTTGTTGCCAAACAAAAAACCATGCAGCGTATTGCAACCGGCACCCCTTCTCGTTcca     >  2:546569/1‑138 (MQ=255)                                                                                                                               cACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATgg  <  1:304280/140‑1 (MQ=255)                                                                                                                               cACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATgg  <  2:2130983/140‑1 (MQ=255)                                                                                                                               cACCATGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATgg  <  2:133789/140‑1 (MQ=255)                                                                                                                                    tGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa      <  2:2253182/131‑1 (MQ=255)                                                                                                                                    tGACCGCACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCa      >  1:2253182/1‑131 (MQ=255)                                                                                                                                          |                                                                                                                                 TCTGATGGGCGGCGGCGCGGGATTTGCACAGCAGCCGCTGTTCTCCTCGAAAAACCCAGCCAGTCCGGCTTACGGTAAATATACCGACGCGACGGGTAAAAACTATGGCGCAGCCCAGCCAGGCCGCACCATGACCGTACCGAAGACGGCAATGGCACCAAAACCGGCGACCACCACTACCGTTACCCGTGGCGGTTTTGGTGAATCTGTTGCCAAACAAAGCACTATGCAGCGTAGTGCAACCGGTACCTCTTCTCGTTCAATGG  >  NC_000913/3180142‑3180407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑