BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,245,550	T→C	G109G (GGT→GGC)	exuT →	hexuronate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,245,550	0	T	C	99.5%	716.2 / NA	200	G109G (GGT→GGC)	exuT	hexuronate transporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (95/104); minor base A (1/0); total (96/104)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 220 total aligned reads displayed.

TAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGG  >  NC_000913/3245414‑3245686

tAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTAGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGcc                                                                                                                                       >  2:532655/1‑140 (MQ=255)

  gCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGcgccgc                                                                                                                                     <  1:1738331/140‑1 (MQ=255)

     ggTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGa                                                                                                                                  <  1:103128/140‑1 (MQ=255)

      gTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGaa                                                                                                                                 >  1:2125258/1‑140 (MQ=255)

      gTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGaa                                                                                                                                 >  2:2298356/1‑140 (MQ=255)

       ttATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAag                                                                                                                                <  1:1409956/140‑1 (MQ=255)

         aTGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAagca                                                                                                                              <  2:1502780/140‑1 (MQ=255)

         aTGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAagca                                                                                                                              <  2:39460/140‑1 (MQ=255)

             aCTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCga                                                                                                                          <  2:1647497/140‑1 (MQ=255)

               tGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCgatg                                                                                                                        <  2:484798/140‑1 (MQ=255)

                ggATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCgatga                                                                                                                       >  1:1676905/1‑140 (MQ=255)

                ggATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTAGCTCGTGTTGCCGTAGGCGCCACGGAAGCAGCgatga                                                                                                                       >  1:640641/1‑140 (MQ=255)

                 gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCgatgat                                                                                                                      >  1:95202/1‑140 (MQ=255)

                 gATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGACCTGGCTGATGCTCGTAGTGCGGTCGGCGCAGCGGAAGCAGCgatgat                                                                                                                      >  2:729729/1‑140 (MQ=255)

                   tgtgCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCACGTGGTGCGGTAGGAGCCGCGGAAGCAGCGATGATcc                                                                                                                    >  1:1067425/1‑140 (MQ=255)

                     tgCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcg                                                                                                                  >  1:1558111/1‑140 (MQ=255)

                     tgCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCAGCGCCGCGGAAGCAGCGATGATCCcg                                                                                                                  >  1:2492883/1‑140 (MQ=255)

                      gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGGTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcgg                                                                                                                 >  2:1409946/1‑140 (MQ=255)

                      gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcgg                                                                                                                 <  1:2298356/140‑1 (MQ=255)

                      gCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcgg                                                                                                                 >  2:578228/1‑140 (MQ=255)

                        tGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcggcg                                                                                                               >  1:2353722/1‑140 (MQ=255)

                         gggTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTTGTGCGGTAGGCGCCGCCGAAGCAGCGATGATCCcggcgg                                                                                                              >  2:944878/1‑140 (MQ=255)

                         gggTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcggcgg                                                                                                              <  2:2347382/140‑1 (MQ=255)

                         gggTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCcggcgg                                                                                                              >  1:2230298/1‑140 (MQ=255)

                           gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCgggg                                                                                                            >  2:2371697/1‑139 (MQ=255)

                           gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGt                                                                                                            >  2:380166/1‑140 (MQ=255)

                           gTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGt                                                                                                            >  2:2072499/1‑140 (MQ=255)

                            tACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTAGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGtt                                                                                                           >  2:327387/1‑139 (MQ=255)

                            tACGAAAATCGGTTAGGCAATGTTTGCTGTGCTGTGGCCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTc                                                                                                           <  1:1116010/140‑1 (MQ=255)

                              cGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTg                                                                                                         <  2:1220095/140‑1 (MQ=255)

                                 aaaTCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGaaa                                                                                                      <  1:1423571/140‑1 (MQ=255)

                                    tCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGTCGCGGAAACAGGGAGGATCCCGGCGGGTCTGAAAGcc                                                                                                   >  1:814565/1‑140 (MQ=255)

                                    tCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAACAGAGACGATCCCGGCGGGTCTGAAAGcc                                                                                                   >  1:1024819/1‑140 (MQ=255)

                                    tCGGTTATGCAATGTTTACTGTTCTGTGGGCCGTGTTCTATGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGcc                                                                                                   <  2:951574/140‑1 (MQ=255)

                                                  tttGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt                                                                                     <  2:1676905/140‑1 (MQ=255)

                                                  tttGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt                                                                                     >  2:182549/1‑140 (MQ=255)

                                                  tttGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt                                                                                     <  1:2035585/140‑1 (MQ=255)

                                                  tttGCTGTGCTGTGGGCCGTGTGCTGTGGTGGAACCGCGCTGGCAGGTCGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGtt                                                                                     <  2:907347/140‑1 (MQ=255)

                                                    tgctgtgctgTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCa                                                                                                  >  2:2477818/1‑125 (MQ=255)

                                                    tgctgtgctgTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTcc                                                                                   <  1:463705/140‑1 (MQ=255)

                                                    tgctgtgctgTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTcc                                                                                   <  1:1380789/140‑1 (MQ=255)

                                                    tgctgtgctgTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTcc                                                                                   >  2:451763/1‑140 (MQ=255)

                                                     gctgtgctgtGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTccc                                                                                  >  1:2404585/1‑140 (MQ=255)

                                                     gctgtgctgtGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGGCCGGAAGCCAGCTCCGAATTGGTccc                                                                                  >  2:59184/1‑140 (MQ=255)

                                                          gctgtgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGa                                                                             >  2:2285083/1‑140 (MQ=255)

                                                           ctgtgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGaa                                                                            >  2:2389814/1‑140 (MQ=255)

                                                            tgtgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGaaa                                                                           <  2:371629/140‑1 (MQ=255)

                                                            tgtgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGCGTGGCCCGGCTGTTGCCCGTGGCGCGGTCGGCGCCGCGGAAGCACCAACGATCCCGGCGCGTCCCAAAGCCCCCCCCCCCCGGTTCCCGGCGaca                                                                           >  2:776351/1‑138 (MQ=255)

                                                              tgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAaga                                                                         >  2:1574335/1‑140 (MQ=255)

                                                              tgGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAaga                                                                         >  2:1481007/1‑140 (MQ=255)

                                                               gggCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAagag                                                                        <  1:451763/140‑1 (MQ=255)

                                                                 gCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCg                                                                      <  2:2247073/140‑1 (MQ=255)

                                                                  ccGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGt                                                                     <  2:1726358/140‑1 (MQ=255)

                                                                  ccGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGt                                                                     <  2:165746/140‑1 (MQ=255)

                                                                   cGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGtt                                                                    >  1:1332484/1‑140 (MQ=255)

                                                                     tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTcc                                                                  >  1:1953350/1‑140 (MQ=255)

                                                                     tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGGCTGAAAGCCATCTCCGAATAGTTCGCGGGGGAAGAGCGTTcc                                                                  >  2:690395/1‑140 (MQ=255)

                                                                     tgtTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGGCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTcc                                                                  >  2:1542257/1‑140 (MQ=255)

                                                                      gtTCTGTGGTGCAACCTCGCTGGCAGGTAGCTGGGATGGACTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCa                                                                 <  2:1127173/140‑1 (MQ=255)

                                                                      gtTCTGTGGTGCAACCGGGCTGTCAGGTAGCCGGGCTGGGCTGGCTGTAGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCa                                                                 <  2:645191/140‑1 (MQ=255)

                                                                      gtTCTGTGGTGCAACCACGCTGGCAGGTAGATGCGGTGGGCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCa                                                                 <  2:1196424/140‑1 (MQ=255)

                                                                       ttCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCAt                                                                <  1:1836877/140‑1 (MQ=255)

                                                                         cTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCg                                                              <  1:1416604/140‑1 (MQ=255)

                                                                         cTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCg                                                              <  1:1513094/140‑1 (MQ=255)

                                                                             ggtgCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCACCGAATGCTTCCCGACGAAAGAGCGGTCCATCGCAGt                                                          >  1:1127173/1‑140 (MQ=255)

                                                                                gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGTCTGTTTCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAgg                                                       <  2:1067425/140‑1 (MQ=255)

                                                                                gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAATAGCGTTCCATCTCAGTAgg                                                       <  2:1441998/140‑1 (MQ=255)

                                                                                gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAgg                                                       <  1:606351/140‑1 (MQ=255)

                                                                                gCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAgg                                                       <  2:2162436/140‑1 (MQ=255)

                                                                                 cAACCGCGCTGGCAGGCAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGGCTGAAAGGCCGCCCCGAATCGTTCCCGGCGGAAGAGCGGTCTATCGCAGTAAGc                                                      >  2:1354729/1‑140 (MQ=255)

                                                                                     cgcgCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACt                                                  <  1:1391031/140‑1 (MQ=255)

                                                                                      gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGTGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACtt                                                 >  1:533705/1‑140 (MQ=255)

                                                                                      gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACtt                                                 >  1:1459205/1‑140 (MQ=255)

                                                                                      gcgcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACtt                                                 <  2:173562/140‑1 (MQ=255)

                                                                                        gcTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACtt                                                 <  1:1729979/138‑1 (MQ=255)

                                                                                            gCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGt                                           <  1:1756262/140‑1 (MQ=255)

                                                                                            gCAGGTAGCTGGGGTGGCCTGGCTGTTGCTAGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGt                                           <  1:327387/140‑1 (MQ=255)

                                                                                                    cTGGGGTGGCCTGGCCGTTGCTTGTCGTGAGGTCGGCCCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGttctt                                   <  1:690395/140‑1 (MQ=255)

                                                                                                      ggggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCg                                 >  1:488565/1‑140 (MQ=255)

                                                                                                       gggTGGCCTGGCTGTTGTTCGTCGTGCGGTCAGCGTCGCCGAAGCAGCCATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGa                                <  2:666671/140‑1 (MQ=255)

                                                                                                        ggTGGCCTGGCTGTTGCTCGTGGTGCGGTCGCCGCTGCGTAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAt                               <  2:965692/140‑1 (MQ=255)

                                                                                                         gTGGCCTGGCTGTTGTTCGTGGTGCTGTCGGCGCCGCTGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAtt                              <  1:940888/140‑1 (MQ=255)

                                                                                                         gTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAtt                              <  2:1315074/140‑1 (MQ=255)

                                                                                                         gTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGAt                               >  1:1817695/1‑139 (MQ=255)

                                                                                                            gCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGt                           >  2:1969831/1‑140 (MQ=255)

                                                                                                            gCCTGGCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCACCGCAGTACGCTACTTTAACGTAGGTTCTTCGATTGGt                           >  1:863291/1‑140 (MQ=255)

                                                                                                                 gCTGTTGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGTAGTCGGCTACTTTAACGTAGGATCTTCGATTGGTGCgat                      >  2:924592/1‑140 (MQ=255)

                                                                                                                      tGCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTg                 >  1:1943734/1‑140 (MQ=255)

                                                                                                                       gCTCGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTgc                >  2:1874532/1‑140 (MQ=255)

                                                                                                                          cGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgcc             <  2:109354/140‑1 (MQ=255)

                                                                                                                          cGTGGTGCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgcc             <  2:2319541/140‑1 (MQ=255)

                                                                                                                           gtggtgCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGTCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACG‑AGGTTCTTCGATTGGTGCGATGATTGcgccgc           <  1:578228/140‑1 (MQ=255)

                                                                                                                           gtggtgCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccg            <  1:1574335/140‑1 (MQ=255)

                                                                                                                           gtggtgCGGTCGGCGCAGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccg            >  2:509727/1‑140 (MQ=255)

                                                                                                                            tggtgCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgc           <  1:393731/140‑1 (MQ=255)

                                                                                                                             ggtgCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGcgccgcc          <  1:97324/140‑1 (MQ=255)

                                                                                                                                gCGGTCGGCGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCt       <  1:120725/140‑1 (MQ=255)

                                                                                                                                 gggtcggcGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtg      <  2:1399745/139‑1 (MQ=255)

                                                                                                                                 cggtcggcGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtg      <  1:1492710/140‑1 (MQ=255)

                                                                                                                                    tcggcGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGTGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCtggtg   >  1:2431927/1‑140 (MQ=255)

TAGCAGGTTATGTACTGGATGTGCTGGGTACGAAAATCGGTTATGCAATGTTTGCTGTGCTGTGGGCCGTGTTCTGTGGTGCAACCGCGCTGGCAGGTAGCTGGGGTGGCCTGGCTGTTGCTCGTGGTGCGGTCGGTGCCGCGGAAGCAGCGATGATCCCGGCGGGTCTGAAAGCCAGCTCCGAATGGTTCCCGGCGAAAGAGCGTTCCATCGCAGTAGGCTACTTTAACGTAGGTTCTTCGATTGGTGCGATGATTGCGCCGCCGCTGGTGG  >  NC_000913/3245414‑3245686

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`