Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,301,885	A→G	D134G (GAC→GGC)	yhbU →	putative peptidase YhbU

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,301,885	0	A	G	99.0%	330.4 / NA	95	D134G (GAC→GGC)	yhbU	putative peptidase YhbU
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (43/51);  total (44/51)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACGCGCTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTG  >  NC_000913/3301752‑3302018                                                                                                                                      |                                                                                                                                      gACGCGCTGATCCTCCCCGACCTCGCCATCCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGc                                                                                                                                 <  1:1114614/140‑1 (MQ=255)      gcTGATCCTCGCCGACCTCGCCATGCTGGGGTACGCCTCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            <  1:961523/140‑1 (MQ=255)      gcTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            <  2:1973291/140‑1 (MQ=255)      gcTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            <  2:2465274/140‑1 (MQ=255)      gcTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            <  2:2085134/140‑1 (MQ=255)        tGATCCTCGCCGACCTCGCCATCCTCGAGCACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCgtg                                                                                                                          <  1:1199168/140‑1 (MQ=255)             cTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGtgct                                                                                                                     <  2:1694955/140‑1 (MQ=255)                 ccGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCcg                                                                                                                 >  2:1369328/1‑140 (MQ=255)                 ccGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCAATTAACTTGTATGATCCCCATTTTGACGTTGCTCGCGTGGTGCTGCcg                                                                                                                 >  2:720723/1‑140 (MQ=255)                 ccGACCTCGCCATGCAGGAGTACGCTGCCGAGCGTTAACCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGTTGCcg                                                                                                                 <  1:1061661/140‑1 (MQ=255)                  cGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGTTTAACTTTTATCTTTGTCATTTTGGGGTTGATCGCGTGGTGTTGCcgc                                                                                                                >  2:1061661/1‑140 (MQ=255)                    aCCTCGCCATGCTGGCGTACGCTGCCGAGAGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCcgcgc                                                                                                              <  1:714375/140‑1 (MQ=255)                    aCCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            <  1:2296745/126‑1 (MQ=255)                    aCCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTcgcg                                                                                                                            >  2:2296745/1‑126 (MQ=255)                    aCCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATAAACTTTTAGCATCGCCATTTTGGCGTTGCTAGCGTGGTGCTGCcgcgc                                                                                                              >  2:814117/1‑140 (MQ=255)                        cGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCgtgt                                                                                                          <  2:2109653/140‑1 (MQ=255)                              gCTGGAGTACGCCGCCGCGCGTCATCCGCATCTTGTGCGCCACGTATCTGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGa                                                                                                    <  2:663926/140‑1 (MQ=255)                                  gAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATtca                                                                                                >  1:1490137/1‑140 (MQ=255)                                   aGTACGCCGCCGAGCGTTATCCGCGTATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATtcat                                                                                               >  1:183706/1‑140 (MQ=255)                                    gTACGCCGCCGAGCGTTATCCGTATATTGAGTGCCGCGTTTCGGTGCAGGCTTCGGCGACTAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATtcatc                                                                                              <  2:829691/140‑1 (MQ=255)                                    gTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATtcatc                                                                                              <  1:2273617/140‑1 (MQ=255)                                       cgccgcCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAgg                                                                                           >  2:1447803/1‑140 (MQ=255)                                       cgccgcCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAgg                                                                                           >  2:440547/1‑140 (MQ=255)                                       cgccgcCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAgg                                                                                           >  2:2190439/1‑140 (MQ=255)                                       cgccgcCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAgg                                                                                           >  1:2245307/1‑140 (MQ=255)                                       cgccgcCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCCCGTGGTGCTGCCGCGCGCGTTGTCGAATCATCAgg                                                                                           >  2:1146407/1‑140 (MQ=255)                                        gccgccGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGt                                                                                          >  2:2149943/1‑140 (MQ=255)                                          cgccgAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGa                                                                                        <  1:1224383/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCTCCACGTCTCTGTGTAGGCTTCTGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:1175350/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCTCCACGTCTCAGTGTAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:1146407/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCTCCACGTCTCAGTGTAGGCTTCAGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:696843/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCTCCACGTATCCGTGTAGGCTTCTGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:814117/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTTGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  2:351265/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:2292398/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:2190439/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  1:2392412/140‑1 (MQ=255)                                                     aTCCGCATATTGAGCGCAACGTCTCGGTGCACGCTACGGCGACCAATGCAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCa                                                                             <  2:799826/140‑1 (MQ=255)                                                      tCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCAc                                                                            >  1:591815/1‑140 (MQ=255)                                                          cATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGt                                                                        <  2:1670023/140‑1 (MQ=255)                                                           atatTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTc                                                                       >  2:541386/1‑140 (MQ=255)                                                           atatTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTc                                                                       >  1:2135331/1‑140 (MQ=255)                                                           atatTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTCGTCGATTCATCAGGTGAAACAACTGGCACGGGTc                                                                       >  2:2132940/1‑140 (MQ=255)                                                            tatTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTca                                                                      <  2:1644512/140‑1 (MQ=255)                                                             atTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATACATGAGCTGAAACTACTGGCACGGGTcac                                                                     >  1:1136836/1‑140 (MQ=255)                                                               tGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCacac                                                                   <  1:2149943/140‑1 (MQ=255)                                                                gAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCAGCGCGTGTCGTCAAATTATCAGGTGAAACAATTGGCACGGGTCACAcc                                                                  >  2:1175350/1‑140 (MQ=255)                                                                 aGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCt                                                                 >  1:1246371/1‑140 (MQ=255)                                                                 aGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTAGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAAATGGCa                                                                             >  2:421205/1‑128 (MQ=255)                                                                 aGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTAGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAAATGGCa                                                                             <  1:421205/128‑1 (MQ=255)                                                                   cgcCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCACGTGTTGTCGATCCATAAGGTGAAACAACTTGCACGGGTCACACCTGt                                                               >  2:913543/1‑140 (MQ=255)                                                                           aTCGGTGCAGGCTTCGGCGACCCATGAAGAGGCGATTTACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTgg                                                       <  1:988303/140‑1 (MQ=255)                                                                                tGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTc                                                  >  1:2155951/1‑140 (MQ=255)                                                                                tGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTc                                                  >  2:1812593/1‑140 (MQ=255)                                                                                 gCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCt                                                 <  1:1447803/140‑1 (MQ=255)                                                                                 gCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCt                                                 <  1:440547/140‑1 (MQ=255)                                                                                  cAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCtt                                                >  2:596846/1‑140 (MQ=255)                                                                                    ggCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTg                                              >  1:1718728/1‑140 (MQ=255)                                                                                          ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCTCGGGTCACACCAGTCCCACTGAAAGTCTTTGCTTTCg                                        >  1:764725/1‑140 (MQ=255)                                                                                          ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTgg                                                       <  2:1473481/125‑1 (MQ=255)                                                                                          ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTgg                                                       >  1:1473481/1‑125 (MQ=255)                                                                                          ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCg                                        >  2:2396742/1‑140 (MQ=255)                                                                                          ggCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCg                                        >  1:1772843/1‑140 (MQ=255)                                                                                           gCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCgg                                       <  1:1419652/140‑1 (MQ=255)                                                                                            cGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGc                                      <  2:591815/140‑1 (MQ=255)                                                                                            cGACCAATAAAGAGGCGATTAATTTTTATCTCCTCGATTGTGGCGTTGATCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGc                                      <  2:1136836/140‑1 (MQ=255)                                                                                              aCCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGATCACACCGGTAGCATTGGAAGACTTTGCTTTCGGCAg                                    >  1:690290/1‑140 (MQ=255)                                                                                               ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCgg                                       <  1:415880/136‑1 (MQ=255)                                                                                               ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCgg                                       >  2:415880/1‑136 (MQ=255)                                                                                               ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                   <  2:1490137/140‑1 (MQ=255)                                                                                               ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                   <  1:1364389/140‑1 (MQ=255)                                                                                               ccAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGc                                   >  2:503083/1‑140 (MQ=255)                                                                                                 aaTGAAGAGGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCt                                 <  2:2155951/140‑1 (MQ=255)                                                                                                      agagGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCa                            <  1:2360461/140‑1 (MQ=255)                                                                                                      agagGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCa                            >  2:128070/1‑140 (MQ=255)                                                                                                      agagGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCa                            <  2:1246371/140‑1 (MQ=255)                                                                                                        agGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCAtt                          <  1:2147960/140‑1 (MQ=255)                                                                                                        agGCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCAtt                          <  2:183706/140‑1 (MQ=255)                                                                                                          gCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                        >  2:77722/1‑140 (MQ=255)                                                                                                          gCGATTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTAt                        >  2:2097405/1‑140 (MQ=255)                                                                                                             aTTAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTc                     >  1:2009384/1‑140 (MQ=255)                                                                                                               tAACTTTTATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCgg                   >  1:3202/1‑140 (MQ=255)                                                                                                                    tttATCATCGCCATTTTTGCGTGGCTCGCGTAGTGATGCTGCGCGTGTCGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGt              <  2:690290/140‑1 (MQ=255)                                                                                                                    tttATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGt              <  2:2245307/140‑1 (MQ=255)                                                                                                                    tttATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGt              >  2:1938016/1‑140 (MQ=255)                                                                                                                    tttATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGt              >  1:2016062/1‑140 (MQ=255)                                                                                                                      tATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCg            <  1:541386/140‑1 (MQ=255)                                                                                                                      tATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCg            <  1:1491626/140‑1 (MQ=255)                                                                                                                      tATCATCGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCg            <  2:1718728/140‑1 (MQ=255)                                                                                                                           tcGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCt       >  2:1841254/1‑140 (MQ=255)                                                                                                                            cGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTa      <  2:2016062/140‑1 (MQ=255)                                                                                                                            cGCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTa      >  2:1289156/1‑140 (MQ=255)                                                                                                                             gCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTACGGCAGCGTGTCCATTATGTCCGAAGGTCGTTGCTAt     >  1:1079627/1‑140 (MQ=255)                                                                                                                             gCCATTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCGTTCGGCAACCTGTGCAGTATGACGGAAGGTCGTTGCTAt     >  2:633065/1‑140 (MQ=255)                                                                                                                                aTTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTg  <  1:596846/140‑1 (MQ=255)                                                                                                                                aTTTTGGCGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTg  <  1:128070/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                      GACGCGCTGATCCTCGCCGACCTCGCCATGCTGGAGTACGCCGCCGAGCGTTATCCGCATATTGAGCGCCACGTATCGGTGCAGGCTTCGGCGACCAATGAAGAGGCGATTAACTTTTATCATCGCCATTTTGACGTTGCTCGCGTGGTGCTGCCGCGCGTGTTGTCGATTCATCAGGTGAAACAACTGGCACGGGTCACACCTGTACCACTGGAAGTCTTTGCTTTCGGCAGCCTGTGCATTATGTCGGAAGGTCGTTGCTATCTG  >  NC_000913/3301752‑3302018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑