BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,348,053	T→C	F30L (TTT→CTT)	npr →	phosphorelay protein Npr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,348,053	0	T	C	98.5%	466.9 / NA	136	F30L (TTT→CTT)	npr	phosphorelay protein Npr
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (63/71); total (64/72)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 141 total aligned reads displayed.

CGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACAGGAAGAGGAAGC  >  NC_000913/3347916‑3348189

cgcgGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGACGCGGTTTGAGTTAATGCAGGGtctt                                                                                                                                        >  1:1038616/1‑140 (MQ=255)

    gTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACg                                                                                                                                    <  2:1806864/140‑1 (MQ=255)

    gTAAAAACGTCCAGTCAAGCCATCGTACGCTCGAAAAACGTAAACCATCACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACg                                                                                                                                    <  2:1038616/140‑1 (MQ=255)

     tAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGc                                                                                                                                   <  2:1379540/140‑1 (MQ=255)

       aaaaCGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTg                                                                                                                                 <  2:603887/140‑1 (MQ=255)

       aaaaCGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTg                                                                                                                                 <  2:2382341/140‑1 (MQ=255)

        aaaCGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGa                                                                                                                                <  2:2004470/140‑1 (MQ=255)

          aCGTCCAGTCACGCCATCGTACGCTGGAATAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAg                                                                                                                              <  1:847077/140‑1 (MQ=255)

            gTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTg                                                                                                                            <  2:1316939/140‑1 (MQ=255)

            gTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACAGTCAAGCAAACTGTTGAAATCACAAACAAGCTTGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTg                                                                                                                            <  2:1303046/140‑1 (MQ=255)

            gTCCAGTCACGCCATCGTACGCGGGAAAAACGTAAACTATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTg                                                                                                                            <  1:967226/140‑1 (MQ=255)

             tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGc                                                                                                                           >  1:352831/1‑140 (MQ=255)

             tCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGc                                                                                                                           <  1:1859673/140‑1 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGaa                                                                                                                               >  1:1587108/1‑134 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGaa                                                                                                                               <  2:1587108/134‑1 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGaa                                                                                                                               <  2:2392497/134‑1 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGaa                                                                                                                               >  1:2392497/1‑134 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGctc                                                                                                                         <  1:1422190/140‑1 (MQ=255)

               cAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCCGCAATGAAGCTGATTGAATTAATGCGGGGTCTTGACGCTGAAGTGctc                                                                                                                         >  2:967226/1‑140 (MQ=255)

                 gTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCtt                                                                                                                       <  1:1883038/140‑1 (MQ=255)

                    aCGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTg                                                                                                                            <  2:2049751/132‑1 (MQ=255)

                    aCGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTg                                                                                                                            >  1:2049751/1‑132 (MQ=255)

                      gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTa                                                                                                                  >  2:1792203/1‑140 (MQ=255)

                      gCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTa                                                                                                                  <  1:2238023/140‑1 (MQ=255)

                           cGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGAc                                                                                                             <  1:404928/140‑1 (MQ=255)

                           cGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGAc                                                                                                             >  2:2133990/1‑140 (MQ=255)

                           cGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGAc                                                                                                             <  1:2471182/140‑1 (MQ=255)

                            gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACg                                                                                                            <  1:1490054/140‑1 (MQ=255)

                            gTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACg                                                                                                            <  1:523628/140‑1 (MQ=255)

                                gCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGAGGCTTAAGTGCTCTTACGTAATGACGAAgg                                                                                                        >  1:818683/1‑140 (MQ=255)

                                 cTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGc                                                                                                       >  1:1494625/1‑140 (MQ=255)

                                   ggAAAAACGTAAACCATGACCGCCAAGCAGACTGTTGGAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCAc                                                                                                     <  1:993074/140‑1 (MQ=255)

                                    gAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCATGACGCTGAAGGGCACTTACGTAATGACGAAGGCAcc                                                                                                    >  1:1194515/1‑140 (MQ=255)

                                      aaaaCGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGa                                                                                                  >  2:1546875/1‑140 (MQ=255)

                                        aaCGTAAACCATGACCGTCAAGCAATCTGCTGAAATCTCAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAgg                                                                                                <  1:677653/140‑1 (MQ=255)

                                        aaCGTAAACCATGACCGACAAGTAAACTGTTGAAACCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAgg                                                                                                <  2:818683/140‑1 (MQ=255)

                                            tAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGa                                                                                            >  1:629556/1‑140 (MQ=255)

                                             aaaCCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGaa                                                                                           <  1:1352899/140‑1 (MQ=255)

                                              aaCCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTTAAGTGCTTTTACGTAATGACGCAGGCACCGAGGCTGAAg                                                                                          >  2:1018787/1‑140 (MQ=255)

                                              aaCCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAg                                                                                          >  2:596709/1‑140 (MQ=255)

                                              aaCCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAg                                                                                          >  2:2171435/1‑140 (MQ=255)

                                                ccATGACCGTCAAGCCAACTGTTGAGATCTCAAACAATCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGcc                                                                                        <  1:1018787/140‑1 (MQ=255)

                                                  aTGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACTCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCaa                                                                                      <  2:1424675/140‑1 (MQ=255)

                                                  aTGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCaa                                                                                      <  1:565274/140‑1 (MQ=255)

                                                    gACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACa                                                                                    >  1:2472628/1‑140 (MQ=255)

                                                         tCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGtt                                                                               >  2:232032/1‑140 (MQ=255)

                                                          cAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTa                                                                              <  2:629556/140‑1 (MQ=255)

                                                           aaGCATACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAAGGTCCAAGGCACCCAGGCTAAAGCCAATAGCGTTAt                                                                             >  2:1120276/1‑140 (MQ=255)

                                                             gCAAACTGTTGAAATCATAAACGAGCTGGGCCTGCTTGCCCGGCCTGCTATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTg                                                                           <  2:739200/140‑1 (MQ=255)

                                                              cAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTgc                                                                          <  1:2361482/140‑1 (MQ=255)

                                                               aaaCTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTgcg                                                                         <  1:1961373/140‑1 (MQ=255)

                                                                aaCTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTgcgc                                                                        <  2:1410554/140‑1 (MQ=255)

                                                                 aCTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACAAAGGCACGGAGACTGAAGCCAACAGCGTTATTGCgct                                                                       >  1:956457/1‑140 (MQ=255)

                                                                   tGTTGAAATCACAAATAAGCTGGGCATGCATGCCCGGGCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCgctgc                                                                     <  1:798683/140‑1 (MQ=255)

                                                                   tGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCgctgc                                                                     <  1:2030837/140‑1 (MQ=255)

                                                                     ttGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGctgctg                                                                   <  1:566168/140‑1 (MQ=255)

                                                                     ttGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGctgctg                                                                   >  2:1428606/1‑140 (MQ=255)

                                                                     ttGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGctgctg                                                                   <  1:2171435/140‑1 (MQ=255)

                                                                       gAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAACGTTATTGCGCTACTGAt                                                                 >  2:2295075/1‑140 (MQ=255)

                                                                         aaTCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGt                                                               >  2:100562/1‑140 (MQ=255)

                                                                         aaTCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGt                                                               >  1:136254/1‑140 (MQ=255)

                                                                             acaaacaaGCTGGGCATGCATGTCCTGCCGGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGa                                                           <  1:1147697/140‑1 (MQ=255)

                                                                             acaaacaaGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGa                                                           <  1:2133990/140‑1 (MQ=255)

                                                                              caaacaaGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGAt                                                          <  1:1894105/140‑1 (MQ=255)

                                                                                    aGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGcc                                                    >  2:2411053/1‑140 (MQ=255)

                                                                                     gCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCa                                                   >  1:1945862/1‑140 (MQ=255)

                                                                                     gCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCa                                                   >  1:2157397/1‑140 (MQ=255)

                                                                                     gCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCa                                                   >  1:2051105/1‑140 (MQ=255)

                                                                                       tGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCaaa                                                 >  1:1313729/1‑140 (MQ=255)

                                                                                        gggCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAg                                                <  2:136254/140‑1 (MQ=255)

                                                                                         ggCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAgg                                               >  2:493354/1‑140 (MQ=255)

                                                                                           catgcatgCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGAc                                             >  2:2019434/1‑140 (MQ=255)

                                                                                            atgcatgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACg                                            <  2:1224120/140‑1 (MQ=255)

                                                                                               catgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGTCAACAGCGCTATGGGGGTGCTGATGATGGACTCTGCCAATGGACGGCa                                         >  2:1113978/1‑140 (MQ=255)

                                                                                               catgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCa                                         >  1:1636743/1‑140 (MQ=255)

                                                                                                atgcCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACgg                                           >  2:2331209/1‑137 (MQ=255)

                                                                                                       gCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTAGGAGTCTGCCAAAGGACGGCAGAttgaag                                 >  1:1076906/1‑140 (MQ=255)

                                                                                                        ccTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGAttgaagt                                >  2:2247323/1‑140 (MQ=255)

                                                                                                        ccTGCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTCATGGAGGATTCTACCAAAAGACGGCACAttgaagt                                >  2:1206775/1‑140 (MQ=255)

                                                                                                           gCAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGAttgaagttga                             <  1:100562/140‑1 (MQ=255)

                                                                                                            cAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAg                                        >  1:1450097/1‑128 (MQ=255)

                                                                                                            cAATGAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAg                                        <  2:1450097/128‑1 (MQ=255)

                                                                                                                gAAGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGa                        <  2:368494/140‑1 (MQ=255)

                                                                                                                 aaGCTGTTTGAATTATTGCAGGGTCGTGATGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGAc                       <  1:1012552/140‑1 (MQ=255)

                                                                                                                 aaGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGAc                       <  2:2145009/140‑1 (MQ=255)

                                                                                                                 aaGCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGAc                       <  2:2051105/140‑1 (MQ=255)

                                                                                                                 aaGCTGTTTGAATTAACCCAGGGTCTTGACGTTGAGGTGCTCTTACGTTATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGAc                       <  2:1076906/140‑1 (MQ=255)

                                                                                                                   gCTGTTTGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCg                     >  1:1781646/1‑140 (MQ=255)

                                                                                                                    cTGTTTGAATTAATGTAGGGTCCTGCCGCTGAAGTGCACTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCgg                    <  1:1113978/140‑1 (MQ=255)

                                                                                                                       tttGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCgg                    <  2:2003775/137‑1 (MQ=255)

                                                                                                                        ttGAATTAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCca                <  1:2411053/140‑1 (MQ=255)

                                                                                                                             ttAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCAGCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACagga           <  1:1775882/140‑1 (MQ=255)

                                                                                                                             ttAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACagga           <  1:2309138/140‑1 (MQ=255)

                                                                                                                             ttAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACagga           <  1:2295075/140‑1 (MQ=255)

                                                                                                                             ttAATGCAGGGTCTTGACGCTGAAGTGCTCTTACGAAATTACGAAGGCCCCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACagga           <  2:956457/140‑1 (MQ=255)

                                                                                                                                 tGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACaggaagag       >  1:2144198/1‑140 (MQ=255)

                                                                                                                                 tGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTCAAGCTGAAGCGACCGGTCCACaggaagag       >  1:1192128/1‑140 (MQ=255)

                                                                                                                                 tGCAGGGTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACAGCAGATTGAAGTTGTAGCGACTGGTCCACaggaagag       >  1:1079079/1‑140 (MQ=255)

                                                                                                                                      ggTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCTGATTGAAGCTGAAGCGGCCGGTGCACAGGACGAGGAAGc  >  2:1159747/1‑140 (MQ=255)

                                                                                                                                      ggTCTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACAGGAAGAGGAAGc  >  1:1376747/1‑140 (MQ=255)

CGCGGTAAAAACGTCCAGTCACGCCATCGTACGCTGGAAAAACGTAAACCATGACCGTCAAGCAAACTGTTGAAATCACAAACAAGCTGGGCATGCATGCCCGGCCTGCAATGAAGCTGTTTGAATTAATGCAGGGTTTTGACGCTGAAGTGCTCTTACGTAATGACGAAGGCACCGAGGCTGAAGCCAACAGCGTTATTGCGCTGCTGATGTTGGATTCTGCCAAAGGACGGCAGATTGAAGTTGAAGCGACCGGTCCACAGGAAGAGGAAGC  >  NC_000913/3347916‑3348189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`