Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,428,808	T→C	intergenic (‑46/‑428)	rrsD ← / → yrdA	16S ribosomal RNA/protein YrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,428,808	0	T	C	100.0%	13.4 / NA	10	intergenic (‑46/‑428)	rrsD/yrdA	16S ribosomal RNA/protein YrdA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/3);  total (7/3)

AGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACT  >  NC_000913/3428780‑3428931                             |                                                                                                                            aGAATTAAACTTCGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGc              >  2:1970679/1‑140 (MQ=14)    aTTAAACTTCGTAATCAATTACCTGCTCACTCTTGAGTCTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCttt           <  1:688610/140‑1 (MQ=255)        aaCTTCGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGc       >  1:1555372/1‑140 (MQ=18)           ttCGTAATGAATTACGTTCTCACTCTTGAGAATTGCTATTCATTTTTCCTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCa    <  2:854812/140‑1 (MQ=11)           ttCGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCa    <  1:303090/140‑1 (MQ=17)             cGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACt  >  1:2015731/1‑140 (MQ=11)             cGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACt  >  1:578654/1‑140 (MQ=11)             cGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACt  >  2:2233049/1‑140 (MQ=11)             cGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTAGCGACGCGGCTTTCAGCTCACt  >  1:1130029/1‑140 (MQ=11)             cGTAATGAATTACGTGCTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAAATGTTAAACAGCAGTAGCGACGCGGCTTTCAGCTCACt  >  1:854812/1‑140 (MQ=255)                             |                                                                                                                            AGAATTAAACTTCGTAATGAATTACGTGTTCACTCTTGAGACTTGGTATTCATTTTTCGTCTTGCGACGTTAAGAATCCGTATCTTCGAGTGCCCACACAGATTGTCTGATAAATTGTTAAAGAGCAGTTGCGACGCGGCTTTCAGCTCACT  >  NC_000913/3428780‑3428931

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑