Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,576,646	T→C	N139S (AAC→AGC)	gntU ←	low‑affinity gluconate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,576,646	0	T	C	100.0%	226.5 / NA	64	N139S (AAC→AGC)	gntU	low‑affinity gluconate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (27/37);  total (27/37)

CACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGTTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGAAGGATT  >  NC_000913/3576511‑3576781                                                                                                                                          |                                                                                                                                        cacaGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGt                                                                                                                                     >  2:2338300/1‑140 (MQ=255) cacaGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGt                                                                                                                                     >  2:1743289/1‑140 (MQ=255)    aGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCACGCGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTa                                                                                                                                    >  2:555724/1‑140 (MQ=255)      gCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCg                                                                                                                                      >  2:479531/1‑134 (MQ=255)      gCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCg                                                                                                                                      <  1:479531/134‑1 (MQ=255)      gCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCgg                                                                                                                                >  2:2255680/1‑140 (MQ=255)      gCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCgg                                                                                                                                >  1:383899/1‑140 (MQ=255)        cAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGgtg                                                                                                                              >  1:411186/1‑140 (MQ=255)          gacAGGATCATCCAGGCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGtgtg                                                                                                                            <  1:1115035/138‑1 (MQ=255)           tCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CACCTGCAAACAATGGGAATACCAGCTCCACCTGGCTCGTACCGGTGTgg                                                                                                                           >  2:742262/1‑140 (MQ=255)            cAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGGTGCGAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGgc                                                                                                                          <  2:1017777/140‑1 (MQ=255)            cAGGATCATCCAGCCAAAGTCGACATTCATCCGCGATGCTAGCAGCATAGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGgc                                                                                                                          <  2:948356/140‑1 (MQ=255)             aGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGgcg                                                                                                                         <  2:383899/140‑1 (MQ=255)             aGGATCACCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGgcg                                                                                                                         <  2:1316133/140‑1 (MQ=255)              ggATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCCGCAAATAAAGGGTTTAGCAGCTTCAGCAGGGCCGTACCGGTGTGgcgc                                                                                                                        >  2:994001/1‑140 (MQ=255)               gATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGgcgcg                                                                                                                       <  1:1727755/140‑1 (MQ=255)                atcatcCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGGAAATAAT‑GGATTCCCAGCTTCACCAAGCTCATACCGGTGTGGCGCGcc                                                                                                                     >  2:793345/1‑140 (MQ=255)                atcatcCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGtgtg                                                                                                                            >  2:608043/1‑134 (MQ=255)                atcatcCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGtgtg                                                                                                                            <  1:608043/134‑1 (MQ=255)                    tcCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAACAATGGGATTACCAGCTGCACCAGGCTCGTAGCGGTGTGGCGCGCCAtt                                                                                                                  >  2:904753/1‑140 (MQ=255)                     ccAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTg                                                                                                                 >  2:2015867/1‑140 (MQ=255)                      cAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTga                                                                                                                <  1:1387005/140‑1 (MQ=255)                          cAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGGTTCACCAGGCTCGTACGGGTGAGGCGCGCCATTgctgtc                                                                                                            >  2:1115035/1‑135 (MQ=255)                           aaaGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAg                                                                                                           <  1:2015867/140‑1 (MQ=255)                           aaaGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAg                                                                                                           <  1:1406621/140‑1 (MQ=255)                           aaaGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAg                                                                                                           <  1:606901/140‑1 (MQ=255)                             aGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCACGCGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAg                                                                                                           <  1:555724/140‑1 (MQ=255)                             aGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCa                                                                                                         >  2:51966/1‑140 (MQ=255)                                    aTTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTaa                                                                                                  <  2:329273/140‑1 (MQ=255)                                     ttCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAAt                                                                                                 <  2:2477625/140‑1 (MQ=255)                                               aTGCCAGCAGCATCGTCGCTGGTCCCGGCTCCAGAAAAGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACt                                                                                       <  1:742262/140‑1 (MQ=255)                                                    agcagcATCGGCGTTGGTCCAGGCACCAGAAAGGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  2:2369938/140‑1 (MQ=255)                                                    agcagcATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  2:2147621/140‑1 (MQ=255)                                                    agcagcATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  1:1837835/140‑1 (MQ=255)                                                    agcagcATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  1:2338300/140‑1 (MQ=255)                                                    agcagcATCGGCGCTGGTCCAGCCATCAGGAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  1:941800/140‑1 (MQ=255)                                                    agcagcATCGGCGCTAGTCCAGACACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGc                                                                                  <  1:904753/140‑1 (MQ=255)                                                           tCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCa                                                                           <  2:1934418/140‑1 (MQ=255)                                                                 cTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAAc                                                                     >  2:207510/1‑140 (MQ=255)                                                                        aGGCACCAGAAATGCTGCCGCTACCCCCA‑‑AGCCTGCACATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTa                                                              <  1:782952/140‑1 (MQ=255)                                                                        aGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTa                                                              <  2:411186/140‑1 (MQ=255)                                                                        aGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTa                                                              <  1:1552232/140‑1 (MQ=255)                                                                        aGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTa                                                              <  1:51966/140‑1 (MQ=255)                                                                             ccAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGca                                                         <  2:322329/140‑1 (MQ=255)                                                                              cAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGcac                                                        >  2:1221630/1‑140 (MQ=255)                                                                              cAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGcac                                                        >  2:1854491/1‑140 (MQ=255)                                                                                  aaTGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGAc                                                    <  2:117449/140‑1 (MQ=255)                                                                                      ccgccgCTGCCGCCA‑‑CGGCTGCGAATAATGGTATTCCCATCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGc                                                <  2:936628/140‑1 (MQ=255)                                                                                        gccgcTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGccc                                              >  2:2391041/1‑140 (MQ=255)                                                                                          cgcTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCg                                            <  1:1743289/140‑1 (MQ=255)                                                                                            cTGCCGCCA‑‑CGCCTGGAAATAATGGTATTCCCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCa                                          <  1:762590/140‑1 (MQ=255)                                                                                            cTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCa                                          <  1:1891119/140‑1 (MQ=255)                                                                                                 gccA‑‑CGCCTGCAAATAAT‑GGATTACTAGCTTCACCAGTCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCg                                    <  1:793345/140‑1 (MQ=255)                                                                                                  ccA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCg                                    <  1:1926492/140‑1 (MQ=255)                                                                                                         ccTGCAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGGGCAGAGACCAGCCTCGCAAGGCCGATGGc                               >  1:723621/1‑140 (MQ=255)                                                                                                             cAAATAATGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGTCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATaa                           >  2:1563593/1‑140 (MQ=255)                                                                                                                    tGGGATTACCAGCTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATgcgcgc                    >  2:383018/1‑140 (MQ=255)                                                                                                                      ggATTACCAGCTTCTCCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCgg                  >  2:2185374/1‑140 (MQ=255)                                                                                                                                cTTCTCCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGa        <  1:2185374/140‑1 (MQ=255)                                                                                                                                cTTCATCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGa        >  2:1834804/1‑140 (MQ=255)                                                                                                                                cTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGa        >  2:2224771/1‑140 (MQ=255)                                                                                                                                cTTCACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGa        >  2:557044/1‑140 (MQ=255)                                                                                                                                   cACCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGAAgg     >  1:1317208/1‑140 (MQ=255)                                                                                                                                    aCCAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGAAGGa    >  2:1296012/1‑140 (MQ=255)                                                                                                                                      cAGGCTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGAAGGAtt  <  1:207510/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        CACAGGCCAATCAGGATCATCCAGCCAAAGTCGGCATTCATCTGCGATGCCAGCAGCATCGGCGCTGGTCCAGGCACCAGAAATGCCGCCGCTGCCGCCA‑‑CGCCTGCAAATAATGGGATTACCAGCTTCACCAGGTTCGTACCGGTGTGGCGCGCCATTGAGAAAGCAACGCTAATCAGCAGAACTATCGCCACTTCAAAGAACAGCGGTAGCGCACAGACCAGCCCCGCAAGGCCGATGGCATAATGCGCGCGGCTGTGACCGAAGGATT  >  NC_000913/3576511‑3576781

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑