BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,625,168	T→C	V264A (GTC→GCC)	yhhI →	putative transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,625,168	0	T	C	99.6%	650.5 / NA	233	V264A (GTC→GCC)	yhhI	putative transposase
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (119/112); total (120/113)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 237 total aligned reads displayed.

AGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAG  >  NC_000913/3625031‑3625301

agagCATGCCAGTTACGTAATCGGTGAAAACAGTCTCGGTAGAGAAGACATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGcct                                                                                                                                     <  2:1180672/140‑1 (MQ=11)

  agCATGACAGTTACGCAATCAGTTAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  1:269133/1‑140 (MQ=16)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGTATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  1:953210/1‑140 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  1:374837/1‑140 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  2:540233/1‑140 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  1:1458471/1‑140 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  2:2178509/1‑140 (MQ=18)

  agCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTAATGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTcc                                                                                                                                   >  1:697002/1‑140 (MQ=18)

     aTGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCttt                                                                                                                                <  2:2458954/140‑1 (MQ=17)

     aTGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCttt                                                                                                                                <  1:1850498/140‑1 (MQ=17)

      tGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCttt                                                                                                                                <  1:2193617/139‑1 (MQ=17)

      tGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTc                                                                                                                               <  1:300231/140‑1 (MQ=17)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  2:2069611/1‑140 (MQ=18)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  2:2155335/1‑140 (MQ=18)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  2:280380/1‑140 (MQ=17)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  2:425912/1‑140 (MQ=18)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  1:1348229/1‑140 (MQ=18)

       gACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCg                                                                                                                              >  1:1650250/1‑140 (MQ=18)

        aCAGTTACGCAATCAGTTAAAACAGTCACGGTAGAGAAGCAATCCGTCGTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCgg                                                                                                                             <  2:688792/140‑1 (MQ=11)

        aCAGTTACGCAATCACTGAAAACAGTCACTGCAGAGACGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCgg                                                                                                                             <  1:767582/140‑1 (MQ=11)

          aGTTACGCAATCAGTGACAAGATTCACGGCAAAGACGAAATCCGTCTTTATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTc                                                                                                                           <  2:774553/140‑1 (MQ=11)

            ttACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTCTGCGTGGCAACCTCCTTTCGGTCCa                                                                                                                         >  1:848728/1‑140 (MQ=16)

            ttACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCgg                                                                                                                             <  1:2168083/136‑1 (MQ=17)

            ttACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCa                                                                                                                         >  1:378780/1‑140 (MQ=18)

            ttACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCa                                                                                                                         >  1:2177587/1‑140 (MQ=18)

             tACGCAATCAGTGAAAAGAGTCCCGCCAGCGAAGAAACCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCat                                                                                                                        <  1:966636/140‑1 (MQ=11)

              aCGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCgg                                                                                                                             >  2:1397171/1‑134 (MQ=18)

                  aaTCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAg                                                                                                                   <  2:2004174/140‑1 (MQ=17)

                  aaTCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAg                                                                                                                   <  1:425927/140‑1 (MQ=17)

                  aaTCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAg                                                                                                                   <  2:2239252/140‑1 (MQ=17)

                    tCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGTTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCa                                                                                                                 >  1:170615/1‑140 (MQ=14)

                    tCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGATATTATGCTTGGCAGGCTCCTTGCGGTCCATAATAGCa                                                                                                                 >  1:746018/1‑140 (MQ=255)

                    tCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCa                                                                                                                 >  1:1967214/1‑140 (MQ=14)

                    tCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCa                                                                                                                 >  1:1832433/1‑140 (MQ=14)

                                           gaagaaATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAAt                                                                                          <  2:1967214/140‑1 (MQ=25)

                                             agaaATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGa                                                                                        <  2:2135153/140‑1 (MQ=25)

                                             agaaATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGa                                                                                        <  2:1668099/140‑1 (MQ=25)

                                                 aTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGTTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                    <  2:170615/140‑1 (MQ=255)

                                                 aTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                    <  2:1458471/140‑1 (MQ=25)

                                                 aTCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                    <  1:2010056/140‑1 (MQ=25)

                                                  tCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCATGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTc                                                                                   <  1:1775066/140‑1 (MQ=25)

                                                   ccGTCTTCATATTGTTTGCGATTTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  <  1:1006334/140‑1 (MQ=21)

                                                   ccGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACTTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAAGGACGGTCa                                                                                  <  2:1375661/140‑1 (MQ=255)

                                                    cGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTGTCGGTCCATAATCGCAGAACAAAAGAAAGATCCAGAAATGACGGTCAg                                                                                 >  2:951546/1‑140 (MQ=255)

                                                     gTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGa                                                                                <  1:230160/140‑1 (MQ=25)

                                                      tCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTc                                                                                   >  2:1812867/1‑136 (MQ=21)

                                                      tCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTc                                                                                   >  2:1840528/1‑136 (MQ=21)

                                                      tCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  >  2:459178/1‑137 (MQ=21)

                                                      tCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  >  1:245806/1‑137 (MQ=21)

                                                                gTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGTCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAg                                                                     <  2:1381927/140‑1 (MQ=21)

                                                                gTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  >  2:2113952/1‑127 (MQ=25)

                                                                gTTTGCGATGTCCCTGATGAACCTATTGATTACACTTTTTAATGGAAACGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAg                                                                     <  2:784260/140‑1 (MQ=11)

                                                                 tttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCTGt                                                                    >  1:2285696/1‑140 (MQ=21)

                                                                 tttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGt                                                                    >  1:225040/1‑140 (MQ=21)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGt                                                                                    <  1:2153724/123‑1 (MQ=25)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                   >  2:2088975/1‑140 (MQ=21)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                   >  2:2202477/1‑140 (MQ=21)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                   <  1:2338518/140‑1 (MQ=25)

                                                                  ttGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGtt                                                                   <  1:484401/140‑1 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  <  2:1666259/124‑1 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  >  2:1306892/1‑124 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCa                                                                                  >  1:1666259/1‑124 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGatat                                                                             <  2:1885956/129‑1 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGatat                                                                             <  2:1091326/129‑1 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGatat                                                                             >  1:1885956/1‑129 (MQ=25)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTc                                                                  >  1:517286/1‑140 (MQ=21)

                                                                   tGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGAAGAACAAATGAACGCGCGAGAAATGACGGTAGGATATTATATCAGTTc                                                                  >  2:1155592/1‑140 (MQ=11)

                                                                    gCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTct                                                                 >  2:2268821/1‑140 (MQ=21)

                                                                     cGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATAACAGTTctg                                                                >  1:61351/1‑140 (MQ=21)

                                                                         gTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGa                                                            >  1:152484/1‑140 (MQ=21)

                                                                         gTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATAACGTTCAGATATTATATCAGTTCTGCTGa                                                            >  1:1090852/1‑140 (MQ=255)

                                                                           cccTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGTAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGTCAGTAATAACGGTCAGGTATTCTATCAGTTCTGCTGAtt                                                          >  2:1199384/1‑140 (MQ=11)

                                                                            ccTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGAttt                                                         <  1:2121606/140‑1 (MQ=21)

                                                                                atgaACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAAcc                                                     >  2:2153515/1‑140 (MQ=21)

                                                                                atgaACTTATTGATTACACGTTAGAGTGGTAAGAGCTCAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAAcc                                                     <  1:726175/140‑1 (MQ=11)

                                                                                    aCTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTg                                                 <  1:2088975/140‑1 (MQ=37)

                                                                                    aCTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGACAGAAATGGCGGACAGGTATTATATAAGTTTTGCTGATTTAACCGCTg                                                 >  2:721396/1‑140 (MQ=11)

                                                                                      ttATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTgag                                               <  1:1389313/140‑1 (MQ=38)

                                                                                       tATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTgaga                                              >  2:303921/1‑140 (MQ=37)

                                                                                                cACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCca                                     >  2:140784/1‑140 (MQ=255)

                                                                                                cACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCca                                     <  1:281198/140‑1 (MQ=255)

                                                                                                      tGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAAtt                                          >  2:377746/1‑129 (MQ=255)

                                                                                                      tGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGACATTATATCAGTTGTGCAGATTTAACGGCTGAGAAATTCGCCACAGCGa                               >  2:636093/1‑140 (MQ=14)

                                                                                                        aaTGGAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTTGCCACAGCGATc                             >  1:2413530/1‑140 (MQ=255)

                                                                                                           ggAAAGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGa                          <  2:2172429/140‑1 (MQ=255)

                                                                                                             aaaGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGaaa                        >  2:1592310/1‑140 (MQ=255)

                                                                                                             aaaGGGCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGaaa                        >  2:488817/1‑140 (MQ=255)

                                                                                                                  gCTGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAAGCGCTGAGAAATTGGCCACAGCGATCCGAAATCACt                   >  2:898917/1‑140 (MQ=255)

                                                                                                                    tGAAGAAATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTgg                 <  1:1309121/140‑1 (MQ=255)

                                                                                                                     gaagaaATTATGCGTTGCAGCCCCCTTTCTGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGc                <  1:1190191/140‑1 (MQ=255)

                                                                                                                      aagaaATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGc                                 <  2:2334963/122‑1 (MQ=255)

                                                                                                                      aagaaATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCa               >  1:1769791/1‑140 (MQ=255)

                                                                                                                      aagaaATTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCa               >  1:2433837/1‑140 (MQ=255)

                                                                                                                           aTTATGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTgg          >  2:308799/1‑140 (MQ=255)

                                                                                                                              aTGCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGgaga       <  2:2486909/140‑1 (MQ=255)

                                                                                                                                gCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAGTTCGCCAAAGCGATCCGAAATCACTGGCACGTGGAGAAt     >  2:740369/1‑140 (MQ=255)

                                                                                                                                gCGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAAt     >  1:13681/1‑140 (MQ=255)

                                                                                                                                 cGTGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATa    <  2:2422575/140‑1 (MQ=255)

                                                                                                                                   tGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAg  <  1:2108537/140‑1 (MQ=255)

                                                                                                                                   tGGCAGCCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCCGAGAAATTCGCCACAGTGATCCGAACTCACGGGCACGTGGAGAATAAg  >  2:1072942/1‑140 (MQ=14)

AGAGCATGACAGTTACGCAATCAGTGAAAAGAGTCACGGCAGAGAAGAAATCCGTCTTCATATTGTTTGCGATGTCCCTGATGAACTTATTGATTTCACGTTTGAATGGAAAGGGCTGAAGAAATTATGCGTGGCAGTCTCCTTTCGGTCCATAATAGCAGAACAAAAGAAAGAGCCAGAAATGACGGTCAGATATTATATCAGTTCTGCTGATTTAACCGCTGAGAAATTCGCCACAGCGATCCGAAATCACTGGCACGTGGAGAATAAG  >  NC_000913/3625031‑3625301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`