BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	407,061	A→G	Y28C (TAC→TGC)	yaiA →	protein YaiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	407,061	0	A	G	99.4%	554.1 / ‑5.2	158	Y28C (TAC→TGC)	yaiA	protein YaiA
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (93/64); total (93/65)
Fisher's exact test for biased strand distribution p-value = 4.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 159 total aligned reads displayed.

GTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTC  >  NC_000913/406925‑407197

gttgATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATAAAGTGACGAATGAGAAAGGAAAGCCAAGACAGACGGTAACCTGGTGCCa                                                                                                                                       >  2:1008563/1‑140 (MQ=255)

   gATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACt                                                                                                                                    <  2:2277273/140‑1 (MQ=255)

     ttttCGAGCGCCTATTCTTAACCTTGATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCa                                                                                                                                  <  1:702637/140‑1 (MQ=255)

        tCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCagag                                                                                                                               >  1:2100066/1‑140 (MQ=255)

           aGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCg                                                                                                                            >  2:83394/1‑140 (MQ=255)

             cgcCTATACTTAACGTTCATCCCGTGAAATATGGACGAACGATGCCAAGGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:771558/140‑1 (MQ=255)

             cgcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  1:615515/140‑1 (MQ=255)

             cgcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:627023/140‑1 (MQ=255)

             cgcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:1392166/140‑1 (MQ=255)

             cgcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:1874513/140‑1 (MQ=255)

             agcCTATACTTAACGTTAATCCCGTGAAATAAGGACGAATGATGCCAACGAAACCGCCTTATCCTCGTAAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:976052/139‑1 (MQ=255)

              gcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          >  1:1851133/1‑139 (MQ=255)

              gcCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:1851133/139‑1 (MQ=255)

               ccTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGatca                                                                                                                        >  1:443478/1‑140 (MQ=255)

               ccTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGatca                                                                                                                        >  1:47237/1‑140 (MQ=255)

               ccTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGatca                                                                                                                        >  1:1237273/1‑140 (MQ=255)

                        aaCGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          >  2:1445319/1‑129 (MQ=255)

                        aaCGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  1:1445319/129‑1 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTCACCTGGTGCCAACTAAGAGCCGATCATCCTAAAccct                                                                                                             >  1:976052/1‑138 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          >  2:2323439/1‑127 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAg                                                                                                             >  1:435265/1‑140 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAg                                                                                                             <  1:143868/140‑1 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAg                                                                                                             >  1:2342860/1‑140 (MQ=255)

                          cGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAg                                                                                                             <  2:435265/140‑1 (MQ=255)

                             tCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGat                                                                                                                          <  2:9521/124‑1 (MQ=255)

                              cATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTc                                                                                                         >  2:1912052/1‑140 (MQ=255)

                              cATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTc                                                                                                         >  1:337624/1‑140 (MQ=255)

                              cATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTc                                                                                                         >  2:1733334/1‑140 (MQ=255)

                               aTCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCg                                                                                                        >  1:1424447/1‑140 (MQ=255)

                               aTCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCg                                                                                                        >  1:1830130/1‑140 (MQ=255)

                               aTCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCg                                                                                                        >  1:1593908/1‑140 (MQ=255)

                                 cccGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGtt                                                                                                      <  2:1494817/140‑1 (MQ=255)

                                 cccGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGtt                                                                                                      <  1:1630614/140‑1 (MQ=255)

                                     tGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAACCGATCTTCCTAAACCAGACTCGTTGATc                                                                                                  >  1:794657/1‑140 (MQ=255)

                                                 gaaCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCg                                                                                      >  1:289928/1‑140 (MQ=255)

                                                  aaCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGa                                                                                     >  1:1064863/1‑140 (MQ=255)

                                                  aaCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGa                                                                                     >  2:2328768/1‑140 (MQ=255)

                                                  aaCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGa                                                                                     >  2:1951460/1‑140 (MQ=255)

                                                  aaCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGa                                                                                     >  1:1379266/1‑140 (MQ=255)

                                                   aCGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGAc                                                                                    <  2:2190785/140‑1 (MQ=255)

                                                     gATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCg                                                                                  <  2:1064863/140‑1 (MQ=255)

                                                        gCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTc                                                                               <  1:1912052/140‑1 (MQ=255)

                                                        gCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTc                                                                               <  1:1951460/140‑1 (MQ=255)

                                                                     ccTTATCCTCGTGAAGCATATACAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCgatgga                                                                  <  1:1134922/140‑1 (MQ=255)

                                                                       ttATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCgatggatg                                                                <  1:536736/140‑1 (MQ=255)

                                                                         aTCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGACAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCg                                                              >  2:509924/1‑140 (MQ=255)

                                                                            cTCGTGAAGCATATAGAGTGACCATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGaaa                                                           <  1:1008563/140‑1 (MQ=255)

                                                                             ttgTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGaaaa                                                          >  1:2239853/3‑140 (MQ=255)

                                                                               gTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAATCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGaaaaaa                                                        >  1:1291420/1‑140 (MQ=255)

                                                                               gTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGaaaaaa                                                        >  1:2287034/1‑140 (MQ=255)

                                                                               gTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGAACATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGTTCAGGAAGCAATGGATGCGaaaaaa                                                        >  1:972309/1‑140 (MQ=255)

                                                                                    gCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCACAGTAAGCGATGGATGCGAAAAAACGCTa                                                   >  1:772049/1‑140 (MQ=255)

                                                                                      atatatAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATg                                                 <  2:1379266/140‑1 (MQ=255)

                                                                                       tatataGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGTCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGa                                                >  2:1828861/1‑140 (MQ=255)

                                                                                        atataGTGACGATTGAAAAAGGTAAGCCAGGGCAGTCGGAAACCTGGTGCCAACTCATAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAg                                               <  2:972309/140‑1 (MQ=255)

                                                                                            aGTGACGAATGAAAAACCAAAGCCAGGACACTCCGTAACCTGGTGCCATCTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGAcc                                           <  2:1202130/140‑1 (MQ=255)

                                                                                              tGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTTAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGAAGGCGATGGATGAGAAAACACGCTATAAGGACCCt                                         >  2:1176748/1‑140 (MQ=255)

                                                                                              tGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCt                                         >  2:1763637/1‑140 (MQ=255)

                                                                                              tGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACAACCGACCGCGCAGAAAGAGATGGATGGGAAAAAACGCTATGAGGACCCt                                         >  2:776331/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATTCGACCGCTCAGGAAGCGATGGATGCTAAAAGACGCTATGAGGACCCTg                                        >  2:885315/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAg                                               <  2:589109/133‑1 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGAcc                                           >  1:2454572/1‑137 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGAcc                                           <  2:2454572/137‑1 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTg                                        >  2:1513674/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTg                                        >  1:1864211/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTg                                        >  2:163394/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTg                                        >  1:139418/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTg                                        >  2:1345506/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAAGAAGCGAAGGAGGCGAAAAAATGCTATGAGGACCCTg                                        >  1:743179/1‑140 (MQ=255)

                                                                                               gACGATTGAAAAAGAAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGAAGGAGGCAAAAAAACGCTATGAGGACCCTg                                        >  1:914722/1‑140 (MQ=255)

                                                                                                aCGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGc                                                     <  2:529700/126‑1 (MQ=255)

                                                                                                aCGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGa                                       >  2:42345/1‑140 (MQ=255)

                                                                                                 cGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGGAGCGATGGATGGGAGAAAACGATATGAGGACCCTGAc                                      >  1:808811/1‑140 (MQ=255)

                                                                                                 cGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGAc                                      >  1:557789/1‑140 (MQ=255)

                                                                                                 cGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGAATGGGAAAAAACGCTATGAGGACCCTGAc                                      >  1:1202130/1‑140 (MQ=255)

                                                                                                  gATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACa                                     >  2:358835/1‑140 (MQ=255)

                                                                                                   aTTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGAGCGCTCAGGCAGCGATGGATGCGAAAGAACGGTATGAGGACCCTGACaa                                    >  2:681723/1‑140 (MQ=255)

                                                                                                   aTTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACaa                                    >  1:1708806/1‑140 (MQ=255)

                                                                                                   aTTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACaa                                    >  1:2142938/1‑140 (MQ=255)

                                                                                                   aTTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACaa                                    >  2:1699911/1‑140 (MQ=255)

                                                                                                     tGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAag                                  >  1:2212061/1‑140 (MQ=255)

                                                                                                          aaGGAAAGCCAGGACAGTCGGTCACCTGGTGCCAACTCATAGCCGATCGTCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGa                             <  2:743179/140‑1 (MQ=255)

                                                                                                          aaGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGa                             <  1:351520/140‑1 (MQ=255)

                                                                                                          aaGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGa                             <  1:163394/140‑1 (MQ=255)

                                                                                                          aaGGAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGa                             <  1:1763637/140‑1 (MQ=255)

                                                                                                            ggAAAGCCAGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGAcc                                           >  2:2254400/1‑124 (MQ=255)

                                                                                                                    aGGACAGACGGTAACTTGGTGCCAACTCAGAGCCGATTATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa                   <  1:991316/140‑1 (MQ=255)

                                                                                                                    aGGACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGa                   <  1:2464076/140‑1 (MQ=255)

                                                                                                                     ggACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGAc                  >  2:325397/1‑140 (MQ=255)

                                                                                                                      gACAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACt                 >  2:2226773/1‑140 (MQ=255)

                                                                                                                        cAGACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGc               <  1:1835986/140‑1 (MQ=255)

                                                                                                                          gACGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTc             >  1:85848/1‑140 (MQ=255)

                                                                                                                           aCGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCg            >  1:23566/1‑140 (MQ=255)

                                                                                                                           aCGGTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGGGACCGCATCAGACTGCTCg            >  2:135311/1‑140 (MQ=255)

                                                                                                                             ggTAACCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGa          <  1:1926148/140‑1 (MQ=255)

                                                                                                                                aaCCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACATAGAATGACCGCATGAGACTGCTCGGAAgg       >  1:800348/1‑140 (MQ=255)

                                                                                                                                 aCCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAggg      >  2:168214/1‑140 (MQ=255)

                                                                                                                                 aCCTGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAggg      <  2:1830130/140‑1 (MQ=255)

                                                                                                                                    tGGTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGAtt   <  1:1699911/140‑1 (MQ=255)

                                                                                                                                     ggTGCCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGGCAAAGAGGGACAGCGTCAGACGGCTCGGAAGGGATTc  >  1:634118/1‑140 (MQ=255)

GTTGATTTTCGAGCGCCTATACTTAACGTTCATCCCGTGAAATAAGGAAGAACGATGCCAACGAAACCGCCTTATCCTCGTGAAGCATATATAGTGACGATTGAAAAAGGAAAGCCAGGACAGACGGTAACCTGGTACCAACTCAGAGCCGATCATCCTAAACCAGACTCGTTGATCAGTGAACATCCGACCGCTCAGGAAGCGATGGATGCGAAAAAACGCTATGAGGACCCTGACAAAGAGTGACCGCATCAGACTGCTCGGAAGGGATTC  >  NC_000913/406925‑407197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`