Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,925,336	C→G	D100H (GAT→CAT)	mnmG ←	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,925,336	0	C	G	100.0%	156.2 / NA	44	D100H (GAT→CAT)	mnmG	5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (39/5);  total (39/5)

TCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTC  >  NC_000913/3925201‑3925469                                                                                                                                         |                                                                                                                                      tCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACGCGATGCGcc                                                                                                                                   <  1:1575133/140‑1 (MQ=255) tCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGcc                                                                                                                                   <  2:608581/140‑1 (MQ=255)     tCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAg                                                                                                                               >  1:978948/1‑140 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCTAGCGCCGTCCGTGCCGACTGACGGTAGAGCCCACGATGCGCCTGAGCt                                                                                                                             >  2:764206/1‑140 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGcc                                                                                                                                   >  1:1649575/1‑134 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGcc                                                                                                                                   <  2:1649575/134‑1 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCt                                                                                                                             >  1:240847/1‑140 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCt                                                                                                                             >  1:12826/1‑140 (MQ=255)       ccATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACAGTCTGACGGTGGAGCCCACGATGCGCCTGAGCt                                                                                                                             >  2:1114332/1‑140 (MQ=255)         aTTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCg                                                                                                                           >  1:2382019/1‑140 (MQ=255)          tttGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCgg                                                                                                                          >  1:2337135/1‑140 (MQ=255)                tAACAGCACCGACCATGCGATCCTTTTC‑GACAATAAGGTCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAgcg                                                                                                                    <  1:1114332/140‑1 (MQ=255)                  aCAGCCCCGACCACGGGATCTTTTCG‑GAAAAAAAGTTCTCCACCCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCCCCAGCGCCGTACGTCCCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGcgcg                                                                                                                  <  2:129063/140‑1 (MQ=255)                  aCAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGcgcg                                                                                                                  >  2:2004520/1‑140 (MQ=255)                     gccccGCCCACGCG‑TCCTTTTCTGAAAAAAAGATCTCAACCCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAAc                                                                                                               <  1:835007/137‑1 (MQ=255)                     gCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAAc                                                                                                               >  1:1596956/1‑140 (MQ=255)                              aCGCGATCGTTTTC‑GACAATAAGATCTTCAACAGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTcc                                                                                                      >  2:294438/1‑140 (MQ=255)                                gcgATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCCCGAGTAGCGAGAACCGCCGGTCCtt                                                                                                    >  1:826815/1‑140 (MQ=255)                                       ttttC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTttgcttgc                                                                                             >  2:576895/1‑140 (MQ=255)                                        tttC‑AAAAAAAAGTTCTACACCCGCCGGCGGGAAGATCATCAGGTTCGGTTGGTTCTCCGGCGCCGTACGTCCCGCCTGAGGGTAGAGCACCGGATGCGCCTGAGCTGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCg                                                                                            <  2:2074555/140‑1 (MQ=255)                                          tC‑GAAAAAAAGTTCTCCACCCGCCGGCGGGAAGATCATCAGGTTCGGTTGGTTCCCCAGCGCCGTACGTCCCGCCTGCCGGTAGAGCACACGATGCGCCTGAGCTGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGtt                                                                                          <  2:1397273/140‑1 (MQ=255)                                             aaaaaTAAGTTTTTAACCCGCCTGCTGGAAGACCATCGGGTTGGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTa                                                                                        <  2:600442/137‑1 (MQ=255)                                                        cTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAAc                                                                             >  2:531308/1‑140 (MQ=255)                                                         ttCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTAGGGTAGCGTGAACCGCCGGTCCTTTGCTTGTGTTTAGTATCCTAAACt                                                                            >  2:845850/1‑140 (MQ=255)                                                           cAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCCCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTgg                                                                          >  2:1043585/1‑140 (MQ=255)                                                             aCCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGAt                                                                        >  1:581240/1‑140 (MQ=255)                                                                gCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAcc                                                                     >  2:613192/1‑140 (MQ=255)                                                                 ccggcgggAAGATCATCAGGTTCGGTTGTTTCCCCGGCGCCGTACGTCCCGCCGGAGGGTAGCGCACACGAGGCGCTTGAGCTCGGGTAGCGCAAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                    <  2:1596956/134‑1 (MQ=255)                                                                 ccTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                    >  1:1375249/1‑140 (MQ=255)                                                                 ccTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                    >  1:537236/1‑140 (MQ=255)                                                                 ccTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAccc                                                                    >  1:2380167/1‑140 (MQ=255)                                                                   tgctgAAAGATCATCAGTTTCGGTTGTTTCTCCGGCGCCGTCCGTCCCGCCTGAGGGAAGAGCACAGGACGCGCCGGAGCTGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGc                                                                  <  2:48221/140‑1 (MQ=255)                                                                               atcaGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTACACTGGATACGCGCCTGATCGATCGc                                                      >  1:1038391/1‑140 (MQ=255)                                                                               atcaGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGc                                                      >  1:383187/1‑140 (MQ=255)                                                                                      tCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGcc                                               >  2:576110/1‑140 (MQ=255)                                                                                       cGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCa                                              >  1:2338058/1‑140 (MQ=255)                                                                                       cGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCa                                              >  1:80902/1‑140 (MQ=255)                                                                                       cGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCa                                              >  2:105418/1‑140 (MQ=255)                                                                                        ggttggttCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAt                                             >  1:1879423/1‑140 (MQ=255)                                                                                                     gcgcCGTACGCCCCCCCTGAGGGTAGACCACACGAGGCGCCTGAGCGGGGGAGGCGCAACCCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGt                                <  2:1879423/140‑1 (MQ=255)                                                                                                     gccccGTACGTCCCCCCGGACGGTAGAGCACACGATGCGCCGGAGCTGGGGTGGCGCAAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGt                                <  1:13321/137‑1 (MQ=255)                                                                                                      cgcCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATTGATAGCATTCGCCGTCAGACCGCCGAGTg                               >  1:1030226/1‑140 (MQ=255)                                                                                                       gcCGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGc                              >  1:1725190/1‑140 (MQ=255)                                                                                                        ccGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCa                             >  1:513913/1‑140 (MQ=255)                                                                                                        ccGTACGTACCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCa                             >  2:2277622/1‑140 (MQ=255)                                                                                                         cgtacgtaCCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACGGGATACCCGCCTGATCAATCGCTTTTGCCATCAGACCGCCGAGTGCAt                            >  2:829147/1‑140 (MQ=255)                                                                                                           tacgtacCGCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACT‑GATACCCGCCTGGTCGATCGCTTTCGCAATCAGACCGCCGAGTGCATCCa                         >  1:1135699/1‑140 (MQ=255)                                                                                                            ccgtccCGCCTGAGGGTAGAGCACCCGATGCGCTGGAGCGGGGGTGGCGCAACCCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCa                         <  2:257521/139‑1 (MQ=255)                                                                                                            ccgtccCCCCTGAGGGAAGAGCACAGGATGCGCTGGGGCGGGGGTGGCGGAACCCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCa                         <  2:581240/139‑1 (MQ=255)                                                                                                            ccgtacCGCCGGGCGGAAGAGCACACAATGCGCCTGAGCTGGGGTGGCGCGACCCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCa                         <  1:2021693/139‑1 (MQ=255)                                                                                                            ccgccccGCCTGACGGTAGAGCCCACGAGGCGCCTGCGCTGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCa                         <  1:294438/135‑1 (MQ=255)                                                                                                                 ccGCCTGAGGGTAGACCACACGTGGTGCCCGAGCTGGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActtct                    <  1:845850/140‑1 (MQ=255)                                                                                                                   gCCTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCttt                  >  1:2070242/1‑140 (MQ=255)                                                                                                                     cTGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTa                >  2:1543520/1‑140 (MQ=255)                                                                                                                      tGACGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTAc               >  2:2227791/1‑140 (MQ=255)                                                                                                                        aCGGTAGAGCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCa             >  1:206724/1‑140 (MQ=255)                                                                                                                          ggaagagCCCACGAGTCCCCTGACCCGGGTTAGCGCATACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGa           <  1:829147/137‑1 (MQ=255)                                                                                                                               agCACAGGATGCGCCTGAGCTGGGGAAGCGCGACCCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTcc      <  1:2012820/140‑1 (MQ=255)                                                                                                                               agCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTcc      >  1:1299472/1‑140 (MQ=255)                                                                                                                               agCACACGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTcc      <  1:105418/140‑1 (MQ=255)                                                                                                                               agCACACGATGCCCCTGAGCCGGGGCAGCCCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTcc      <  1:1080472/140‑1 (MQ=255)                                                                                                                                   cacGATGCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTc  >  1:285956/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                      TCAGTCCCATTTGGGTAACAGCACCGACCACGCGATCGTTTTC‑GACAATAAGATCTTCAACCGCCTGCTGGAAGATCATCAGGTTCGGTTGGTTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTC  >  NC_000913/3925201‑3925469

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑