BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,956,738	A→G	T470A (ACC→GCC)	ilvA →	threonine deaminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,956,738	0	A	G	98.4%	427.1 / ‑2.9	124	T470A (ACC→GCC)	ilvA	threonine deaminase
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (72/50); total (73/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.41e-01

Only 100 of 127 total aligned reads displayed.

GCGGACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTA  >  NC_000913/3956601‑3956874

gcggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGcc                                                                                                                                        >  2:1797239/1‑140 (MQ=255)

gcggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGcc                                                                                                                                        >  1:1494886/1‑140 (MQ=255)

gcggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGcc                                                                                                                                        >  2:1295907/1‑140 (MQ=255)

gcggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGcc                                                                                                                                        >  1:1747038/1‑140 (MQ=255)

gcggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTAATGGTACAGTTCTTTGTTCCACTATCGCAGCCATGGCGcc                                                                                                                                        >  2:821628/1‑140 (MQ=255)

  ggACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGa                                                                                                                                      >  1:1481315/1‑140 (MQ=255)

        ccATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACgg                                                                                                                                <  1:1948799/140‑1 (MQ=255)

                 ccGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACt                                                                                                                       >  2:2308618/1‑140 (MQ=255)

                  cGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCCCTATCGCTGCCATGGCGCCGAGTACGGGCGCGTACTg                                                                                                                      >  1:746205/1‑140 (MQ=255)

                  cGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTg                                                                                                                      >  1:1718724/1‑140 (MQ=255)

                    ttGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATAACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTggc                                                                                                                    <  1:1066150/140‑1 (MQ=255)

                     tGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTCTCGCAGCTATGACGTCGACTATGGGCGCGTACCggcg                                                                                                                   >  1:1173292/1‑140 (MQ=255)

                       cAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATGGCAGCCATGGCGCCGACTACGGGCGCGTACTggcggc                                                                                                                 >  1:918744/1‑140 (MQ=255)

                       cAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTAGTTCCACTGTCGTAGCAATGGCGCCAACTATGGGCGCGTACTggcggc                                                                                                                 >  2:717451/1‑140 (MQ=255)

                         ggAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGt                                                                                                               >  1:2239676/1‑140 (MQ=255)

                         ggAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGt                                                                                                               >  1:2402774/1‑140 (MQ=255)

                         ggAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGt                                                                                                               >  1:1633772/1‑140 (MQ=255)

                         ggAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGAAGCCATGTCGCGGAGTACGGGTGCGTACTGGCGGCGt                                                                                                               >  1:664974/1‑140 (MQ=255)

                                  ctACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATCGCGCCGATTACGGGCGTGTACTGGCGGCGTTCGAACTTg                                                                                                      >  2:1170290/1‑140 (MQ=255)

                                   tACAGCTTCGAATTCCCGGAATAACCGGGTGCGCTGCCGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTgg                                                                                                     <  1:910118/140‑1 (MQ=255)

                                    aCAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGc                                                                                                    <  1:1592940/140‑1 (MQ=255)

                                    aCAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGc                                                                                                    <  2:1786333/140‑1 (MQ=255)

                                        cTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGAcc                                                                                                >  2:2184173/1‑140 (MQ=255)

                                         ttCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACCACGGGCGGGTAATGACGGCGTACGAACTTGGCGACCa                                                                                               >  1:828406/1‑140 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGGGGCGTTCGAACTTGGCGACCAt                                                                                              >  1:1085280/1‑140 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGaa                                                                                                          <  2:2459035/128‑1 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCAt                                                                                              >  2:420747/1‑140 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCAt                                                                                              >  2:1265826/1‑140 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCAt                                                                                              >  1:1633353/1‑140 (MQ=255)

                                          tCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCAt                                                                                              >  1:33057/1‑140 (MQ=255)

                                           cGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATg                                                                                             <  1:99779/140‑1 (MQ=255)

                                             aaTTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGaa                                                                                           >  1:2353863/1‑140 (MQ=255)

                                                tCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCg                                                                                        <  2:1494886/140‑1 (MQ=255)

                                                 cccGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCgg                                                                                       <  1:2308618/140‑1 (MQ=255)

                                                  ccGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGa                                                                                      <  2:33057/140‑1 (MQ=255)

                                                     gAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGAttt                                                                                   <  2:2353863/140‑1 (MQ=255)

                                                           ccGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAAc                                                                             <  2:593506/140‑1 (MQ=255)

                                                             gggCGCGCTGCTGCGCCCCCTCGACACGCTGGGCACGTACTGGAACATTTCTTTGTTCCACTTTCGCAGCCATGGCGCCGACTACGGGCGCGTACCGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAAccc                                                                           <  2:1173292/140‑1 (MQ=255)

                                                              ggCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCg                                                                          <  1:1745650/140‑1 (MQ=255)

                                                                cgcgcTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGc                                                                        >  2:1460792/1‑140 (MQ=255)

                                                                cgcgcTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGCTCGTACTAGGCGACCAGGAACCGGATTTCGAAACCCGGc                                                                        >  2:762293/1‑140 (MQ=255)

                                                                  cgcTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTg                                                                      >  2:2126286/1‑140 (MQ=255)

                                                                  cgcTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTg                                                                      >  2:1536646/1‑140 (MQ=255)

                                                                   gctgctGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGa                                                                     <  1:1460792/140‑1 (MQ=255)

                                                                    ctgctgCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGaa                                                                    >  1:2339984/1‑140 (MQ=255)

                                                                        tgcGCTTCCTCAACACGGTGGGAACGTACTGAAACATTTGTTTGTTCCTCTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAg                                                                <  2:862977/140‑1 (MQ=255)

                                                                         gcgcTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGc                                                               <  2:572342/140‑1 (MQ=255)

                                                                          cgcTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCt                                                              <  2:2402774/140‑1 (MQ=255)

                                                                               ccTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCt                                                         >  1:1699863/1‑140 (MQ=255)

                                                                               ccTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCt                                                         >  2:270837/1‑140 (MQ=255)

                                                                               ccTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCt                                                         >  2:1880342/1‑140 (MQ=255)

                                                                                 tCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTAc                                                       >  2:1682399/1‑140 (MQ=255)

                                                                                 tCAACACGCTGGATACGTACTGGAACATTTCTTTGTTCGACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGTGGCGTTCGATAATGGCGACCATGATCAGAATTTCGAGACGCGGCTGAGAAAGCCGGGCTAc                                                       >  2:1541429/1‑140 (MQ=255)

                                                                                    acacGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGAt                                                    >  1:47802/1‑140 (MQ=255)

                                                                                        gCTGGGTATGTACTGGATCATTACTTTGTTCCACTATCGAAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGcc                                                <  2:1156114/140‑1 (MQ=255)

                                                                                         cTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAg                                                                <  2:2096803/123‑1 (MQ=255)

                                                                                         cTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAg                                                                >  1:2096803/1‑123 (MQ=255)

                                                                                          tGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCAGGAACCGGAATTCGAAAGCCGGCTGAATGAGGTGGGCTACGATTGCCac                                              >  2:696142/1‑140 (MQ=255)

                                                                                           gggTACGTATCGGAACATTTCTTTGTTCCACTATCGCAGCCATCACGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCacg                                             <  1:1541429/140‑1 (MQ=255)

                                                                                           gggTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCacg                                             >  2:618131/1‑140 (MQ=255)

                                                                                               acgtacTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCAcgacga                                         <  1:1131514/140‑1 (MQ=255)

                                                                                               acgtacTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCAcgacga                                         <  1:1923003/140‑1 (MQ=255)

                                                                                               acgtacTGGAACATTCCTTTGTCCCTCTACCGCAGCCTTGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCAcgacga                                         <  1:696142/140‑1 (MQ=255)

                                                                                                cgtacTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGaa                                        <  1:420747/140‑1 (MQ=255)

                                                                                                  tacTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAAACCGGCCGTATGAGCTGGGCTACGATTGCCACGACGAAAc                                      >  2:807239/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTGGAAACCCGGCTGTATGAGCTGGGCTGCGATGGCCACGACGAAACCAAt                                  >  2:678308/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTGGAAACCCGACTGAATGAGCTGGGCTGCGATTGCCACGACGAAACCAAt                                  >  2:705416/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt                                  >  2:1522938/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt                                  >  2:1944770/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt                                  >  2:246071/1‑140 (MQ=255)

                                                                                                      ggAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAAt                                  >  1:52817/1‑140 (MQ=255)

                                                                                                       gAACATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATa                                 >  1:2020671/1‑140 (MQ=255)

                                                                                                        aaCATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATaa                                >  1:117192/1‑140 (MQ=255)

                                                                                                        aaCATTTCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATaa                                >  1:459211/1‑140 (MQ=255)

                                                                                                            tttCTTTGTTCCACTGTCGCAGTCATGGCTCCGACTAGGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCg                            <  1:1170290/140‑1 (MQ=255)

                                                                                                            tttCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCg                            >  1:2086164/1‑140 (MQ=255)

                                                                                                             ttCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCgg                           >  2:1488187/1‑140 (MQ=255)

                                                                                                             ttCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCgg                           >  2:2369649/1‑140 (MQ=255)

                                                                                                             ttCTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCAGGCTGAATTAGCCGGGATACGATTGTCACGACGAAACCAATAACCCgg                           >  2:867718/1‑140 (MQ=255)

                                                                                                             gtCTTTGTTCCACTAGCGCAGCTATAGCGGCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCgg                           <  1:1140552/139‑1 (MQ=255)

                                                                                                               cTTTGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCg                         <  2:1349802/140‑1 (MQ=255)

                                                                                                                tttGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGt                        <  2:1633353/140‑1 (MQ=255)

                                                                                                                 ttGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGtt                       <  1:1246508/140‑1 (MQ=255)

                                                                                                                 ttGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGtt                       <  2:52817/140‑1 (MQ=255)

                                                                                                                  tGTTCCACTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTc                      >  2:259717/1‑140 (MQ=255)

                                                                                                                         cTATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCt               >  1:224411/1‑140 (MQ=255)

                                                                                                                          tATCGCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCtt              >  2:287413/1‑140 (MQ=255)

                                                                                                                              gCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTg          <  1:2184173/140‑1 (MQ=255)

                                                                                                                              gCAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTg          <  2:1747038/140‑1 (MQ=255)

                                                                                                                               cAGCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCATGACGAAAACAAGAAACCGGCGGTCAGGTTCTTTTTgg         >  1:776104/1‑140 (MQ=255)

                                                                                                                                aGCCATGGCGCCGACTATGGGCTCGTACTGGAGGCTTTCTAACTTGGCTACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGc        <  2:918744/140‑1 (MQ=255)

                                                                                                                                 gCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTgg         <  2:2005155/138‑1 (MQ=255)

                                                                                                                                 gCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTgg         >  1:2005155/1‑138 (MQ=255)

                                                                                                                                 gCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCg       >  2:1718368/1‑140 (MQ=255)

                                                                                                                                 gCCATGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCg       <  1:287413/140‑1 (MQ=255)

                                                                                                                                    aTGGCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGt    >  2:2125283/1‑140 (MQ=255)

                                                                                                                                     tGGCGCCGACTACGGGCGCGTACTGGTGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGtt   <  2:1576236/140‑1 (MQ=255)

                                                                                                                                      ggCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGt    <  1:2116295/138‑1 (MQ=255)

                                                                                                                                      ggCGCCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTa  <  1:262600/140‑1 (MQ=255)

                                                                                                                                      ggCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTa  >  1:2191805/1‑140 (MQ=255)

GCGGACGTCCATCGCATCCGTTGCAGGAACGCCTCTACAGCTTCGAATTCCCGGAATCACCGGGCGCGCTGCTGCGCTTCCTCAACACGCTGGGTACGTACTGGAACATTTCTTTGTTCCACTATCGCAGCCATGGCACCGACTACGGGCGCGTACTGGCGGCGTTCGAACTTGGCGACCATGAACCGGATTTCGAAACCCGGCTGAATGAGCTGGGCTACGATTGCCACGACGAAACCAATAACCCGGCGTTCAGGTTCTTTTTGGCGGGTTA  >  NC_000913/3956601‑3956874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`