BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,976,956	A→G	T227A (ACG→GCG)	wzxE →	lipid III flippase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,976,956	0	A	G	99.4%	618.4 / ‑5.6	173	T227A (ACG→GCG)	wzxE	lipid III flippase
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (66/106); total (66/107)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 174 total aligned reads displayed.

ATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTT  >  NC_000913/3976821‑3977092

 ttCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCg                                                                                                                                     <  2:2397924/140‑1 (MQ=255)

     cgcgCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGa                                                                                                                                 >  2:1888007/1‑140 (MQ=255)

      gcgcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGAc                                                                                                                                <  1:529120/140‑1 (MQ=255)

        gcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCt                                                                                                                              <  2:2097419/140‑1 (MQ=255)

        gcTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCt                                                                                                                              >  2:140083/1‑140 (MQ=255)

           ggtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGc                                                                                                                           <  2:1361505/140‑1 (MQ=255)

            gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGcc                                                                                                                          >  1:2453265/1‑140 (MQ=255)

            gtggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGcc                                                                                                                          >  2:309622/1‑140 (MQ=255)

             tggtAATTCCTGCCGCCGTCATATTGATCAAACGTGGTGCCATCCCGTGAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGACAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCt                                                                                                                         <  2:813630/140‑1 (MQ=255)

             tggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCt                                                                                                                         <  2:2429759/140‑1 (MQ=255)

             tggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGACAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCt                                                                                                                         <  2:2037370/140‑1 (MQ=255)

             tggtAATTCCTGCCGCCATCATCTTTATCCAACGTGGGGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCt                                                                                                                         <  1:784345/140‑1 (MQ=255)

              ggtAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTg                                                                                                                        <  1:2105288/140‑1 (MQ=255)

                  aTTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGc                                                                                                                    <  1:1629984/140‑1 (MQ=255)

                  aTTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGc                                                                                                                    <  2:2183888/140‑1 (MQ=255)

                  aTTCCTGCCGCCATCATGTTGACCAAACGTGGTGTCATCGCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGc                                                                                                                    <  2:761341/140‑1 (MQ=255)

                  aTTCCTGCCGCCATCATGTTGAACAAACGTGGTGTCATCACGTTAAGCCATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGc                                                                                                                    <  2:934077/140‑1 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCt                                                                                                                   <  2:585684/137‑1 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCt                                                                                                                   >  1:585684/1‑137 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAc                                                                                                                >  2:246545/1‑140 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAc                                                                                                                >  1:1485320/1‑140 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAc                                                                                                                <  2:57640/140‑1 (MQ=255)

                      cTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAc                                                                                                                >  2:119329/1‑140 (MQ=255)

                       tGCCGCCATCATGTTGATCAAACGCGGCGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAca                                                                                                               >  1:343120/1‑140 (MQ=255)

                        gccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGTCGTTGATTGCGACGATGACCTGGCCTGTTGCTTAcat                                                                                                              >  1:1206760/1‑140 (MQ=255)

                        gccgccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGc                                                                                                                    <  2:60732/134‑1 (MQ=255)

                           gccATCATGTTGATCAAACGTGTTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAcatcat                                                                                                           <  1:863303/140‑1 (MQ=255)

                           gccATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTAcatcat                                                                                                           <  2:1296233/140‑1 (MQ=255)

                              atcatGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCatgat                                                                                                        <  1:140083/140‑1 (MQ=255)

                                   gTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTa                                                                                                   >  2:1694635/1‑140 (MQ=255)

                                       aTCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAAct                                                                                               >  1:2056566/1‑140 (MQ=255)

                                         cAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgc                                                                                             >  1:492001/1‑140 (MQ=255)

                                         cAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAActgc                                                                                             >  1:1777493/1‑140 (MQ=255)

                                            aCGTGGTGCCATCCCGTCAAGCAATCTGAAATCCATCTGTGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTgg                                                                                          <  2:1210161/140‑1 (MQ=255)

                                             cGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggc                                                                                         >  1:616592/1‑140 (MQ=255)

                                              gtggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcg                                                                                        >  2:1346603/1‑140 (MQ=255)

                                               tggtgTCATCCCGTTAAGCTATCTGAAACCCAGCTGGAATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                                                       <  1:1019204/140‑1 (MQ=255)

                                               tggtgTCAGCCCGTTATTCTATATGAACCCACGGTAGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATTATGCGTAAACTGCTggcgg                                                                                       <  2:1206745/140‑1 (MQ=255)

                                               tggtgTCAACCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTTGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTggcgg                                                                                       <  2:1171630/140‑1 (MQ=255)

                                                 gtgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGgcggcg                                                                                     >  1:1670998/1‑140 (MQ=255)

                                                  tgtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCAGTTGCTTACATAATGATGCGTAAACTGCTGGCGgcgc                                                                                    >  1:936789/1‑140 (MQ=255)

                                                   gtCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCa                                                                                   >  1:329573/1‑140 (MQ=255)

                                                    tCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAg                                                                                  <  2:1670998/140‑1 (MQ=255)

                                                    tCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAg                                                                                  >  1:1759201/1‑140 (MQ=255)

                                                           gTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCt                                                                           <  1:1814690/140‑1 (MQ=255)

                                                           gTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCt                                                                           <  1:1346603/140‑1 (MQ=255)

                                                               aGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                                                       <  2:1661401/140‑1 (MQ=255)

                                                                   aTCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAGTTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGa                                                                       <  1:2077140/136‑1 (MQ=255)

                                                                    tCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAgg                                                                  <  2:1485320/140‑1 (MQ=255)

                                                                     cTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGt                                                                 <  2:492001/140‑1 (MQ=255)

                                                                     cTGAAACCCAGCTGGGATAACGGTCGGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGt                                                                 <  1:1021853/140‑1 (MQ=255)

                                                                      tGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTg                                                                >  1:2239073/1‑140 (MQ=255)

                                                                              aGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTg                                                        >  1:1059717/1‑140 (MQ=255)

                                                                              aGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACCGCTCGCGTCGCAGTATAGCTGGGATGAGGTGGGGATCTg                                                        >  2:829744/1‑140 (MQ=255)

                                                                              aGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGAGGCGTAAACGGCTTGCGCCGCAGTATTGCTGAGATGAGGTGGGGATCTg                                                        >  2:694155/1‑140 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      >  2:1245652/1‑140 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      <  2:2056566/140‑1 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      <  2:1860852/140‑1 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      <  2:1777493/140‑1 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      <  2:174082/140‑1 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      >  2:1489654/1‑140 (MQ=255)

                                                                                cTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGc                                                      >  2:1533367/1‑140 (MQ=255)

                                                                                     aTAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAggg                                                 <  2:2260788/140‑1 (MQ=255)

                                                                                     aTAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAggg                                                 <  1:1817250/140‑1 (MQ=255)

                                                                                        aCGGTCTGGCAGGGCAGTTGAGCAAGTTTTCGCTCATAGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg                                              <  1:755627/140‑1 (MQ=255)

                                                                                        aCGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg                                              <  1:1357263/140‑1 (MQ=255)

                                                                                        aCGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg                                              <  1:570883/140‑1 (MQ=255)

                                                                                        aCGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTg                                              <  1:2409144/140‑1 (MQ=255)

                                                                                          ggTCTGGCAGGGCAGTTGAGCAGATATACTCTCATGGTGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg                                            <  1:829744/140‑1 (MQ=255)

                                                                                          ggTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg                                            <  1:1888007/140‑1 (MQ=255)

                                                                                          ggTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg                                            <  1:1273864/140‑1 (MQ=255)

                                                                                           gTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGc                                           <  1:623377/140‑1 (MQ=255)

                                                                                           gTCTGGCAGGGCAGTCGAGCAACTTCACGATCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGc                                           <  1:1137916/140‑1 (MQ=255)

                                                                                              tGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGt                                               >  1:1751731/1‑133 (MQ=255)

                                                                                              tGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg                                            <  2:409442/136‑1 (MQ=255)

                                                                                              tGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAg                                            >  1:409442/1‑136 (MQ=255)

                                                                                                   gggcagTTGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTc                                   <  1:1694803/140‑1 (MQ=255)

                                                                                                         ttGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGc                             <  2:29843/140‑1 (MQ=255)

                                                                                                          tGAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGcc                            >  1:2236687/1‑140 (MQ=255)

                                                                                                           gAGCAAATTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCt                           <  2:161850/140‑1 (MQ=255)

                                                                                                                aaTTTACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTg                      <  2:1447697/140‑1 (MQ=255)

                                                                                                                  tttACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCa                    <  2:128150/140‑1 (MQ=255)

                                                                                                                   ttACGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCaa                   <  2:1759201/140‑1 (MQ=255)

                                                                                                                     aCGCTCATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAAtt                 <  2:1059717/140‑1 (MQ=255)

                                                                                                                          cATGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATttatta            <  2:616592/140‑1 (MQ=255)

                                                                                                                           aTGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCt                           <  2:476114/124‑1 (MQ=255)

                                                                                                                           aTGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCt                           >  1:476114/1‑124 (MQ=255)

                                                                                                                           aTGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATttatta            >  1:28122/1‑139 (MQ=255)

                                                                                                                           aTGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTAc           >  2:2179311/1‑140 (MQ=255)

                                                                                                                           aTGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTAc           >  1:2288288/1‑140 (MQ=255)

                                                                                                                            tGGCGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACg          <  2:2288288/140‑1 (MQ=255)

                                                                                                                               cGTTGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCa       <  1:1245652/140‑1 (MQ=255)

                                                                                                                                 ttGATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATc     >  2:2488445/1‑140 (MQ=255)

                                                                                                                                   gATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGt   <  1:1533367/140‑1 (MQ=255)

                                                                                                                                   gATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGt   <  2:2236687/140‑1 (MQ=255)

                                                                                                                                   gATTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGt   <  1:1800185/140‑1 (MQ=255)

                                                                                                                                    aTTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGtt  <  2:2453265/140‑1 (MQ=255)

                                                                                                                                    aTTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGtt  <  1:2488445/140‑1 (MQ=255)

                                                                                                                                    aTTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGtt  <  1:2455358/140‑1 (MQ=255)

                                                                                                                                    aTTGCGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGtt  <  1:2203185/140‑1 (MQ=255)

ATTCCCGCGCTGGTGGTAATTCCTGCCGCCATCATGTTGATCAAACGTGGTGTCATCCCGTTAAGCTATCTGAAACCCAGCTGGGATAACGGTCTGGCAGGGCAGTTGAGCAAATTTACGCTCATGGCGTTGATTACGTCGGTGACCTTGCCTGTTGCTTACATCATGATGCGTAAACTGCTGGCGGCGCAGTATAGCTGGGATGAGGTGGGGATCTGGCAAGGGGTGAGCAGTATTTCCGATGCCTACCTGCAATTTATTACGGCATCGTT  >  NC_000913/3976821‑3977092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`