BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,032,000	A→G	E280E (GAA→GAG)	pepQ →	Xaa‑Pro dipeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,032,000	0	A	G	99.2%	442.4 / NA	127	E280E (GAA→GAG)	pepQ	Xaa‑Pro dipeptidase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (57/69); minor base C (0/1); total (57/70)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 129 total aligned reads displayed.

CAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAA  >  NC_000913/4031863‑4032135

cAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGagca                                                                                                                                       >  2:141132/1‑140 (MQ=255)

 aGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGagcaa                                                                                                                                      <  2:1388053/140‑1 (MQ=255)

       cGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGgcg                                                                                                                                <  1:92526/140‑1 (MQ=255)

       cGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGgcg                                                                                                                                <  2:1671107/140‑1 (MQ=255)

       cGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGgcg                                                                                                                                <  2:180570/140‑1 (MQ=255)

       cGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGgcg                                                                                                                                >  2:2282619/1‑140 (MQ=255)

        ggAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGgcgc                                                                                                                               >  2:131970/1‑140 (MQ=255)

         gAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACATCGACTACGTGCACCTGCTGAAGGACCTTAATGATGAGCAACTGGCGCt                                                                                                                              >  2:952583/1‑140 (MQ=255)

         gAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCt                                                                                                                              >  2:2412778/1‑140 (MQ=255)

         gAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCt                                                                                                                              >  1:40916/1‑140 (MQ=255)

             agaTGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGTGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATc                                                                                                                          >  2:1781136/1‑140 (MQ=255)

               aTGCGCAGCTTCCTGCTGGATGGCGGGGCAGTATATAACGGCTATGCGCCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGc                                                                                                                        <  2:790564/140‑1 (MQ=255)

               aTGCGCAGCTTCCTGCTAGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGc                                                                                                                        <  2:1058712/140‑1 (MQ=255)

                 gcgcAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACGTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGcca                                                                                                                      >  2:1479810/1‑140 (MQ=255)

                   gcAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGccacc                                                                                                                    >  1:348935/1‑140 (MQ=255)

                   gcAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGccacc                                                                                                                    <  1:1853084/140‑1 (MQ=255)

                    cAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGc                                                                                                                        <  2:465670/135‑1 (MQ=255)

                    cAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGc                                                                                                                        >  1:465670/1‑135 (MQ=255)

                     aGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCAt                                                                                                                  <  2:348935/140‑1 (MQ=255)

                     aGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCAt                                                                                                                  <  2:343242/140‑1 (MQ=255)

                      gCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATg                                                                                                                 >  1:1887404/1‑140 (MQ=255)

                               tgGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGc                                                                                                        <  2:291418/140‑1 (MQ=255)

                                 gATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGt                                                                                                      <  2:2104072/140‑1 (MQ=255)

                                   tGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCa                                                                                                    >  2:1321682/1‑140 (MQ=255)

                                    gCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAg                                                                                                   <  1:1321682/140‑1 (MQ=255)

                                      cGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCt                                                                                                 <  2:16734/140‑1 (MQ=255)

                                      cGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCt                                                                                                 <  1:141132/140‑1 (MQ=255)

                                        gggCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAt                                                                                               <  1:131970/140‑1 (MQ=255)

                                        gggCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAt                                                                                               <  2:62185/140‑1 (MQ=255)

                                        gggCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAt                                                                                               <  2:1452557/140‑1 (MQ=255)

                                         ggCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAtg                                                                                              <  1:2282619/140‑1 (MQ=255)

                                         ggCAGAATATAACGGCTATGCGGCAGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAtg                                                                                              <  1:2067498/140‑1 (MQ=255)

                                             gAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGa                                                                                          <  1:1746150/140‑1 (MQ=255)

                                             gAATATAACGGCTATCCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGa                                                                                          <  1:845231/140‑1 (MQ=255)

                                                tataACGGCTATGCGGCAGACCTGACCCGTAACTGATCGCCAAAAAGTAACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGC‑GGCGTCAGCTATGTGGATTAc                                                                                      <  2:752949/140‑1 (MQ=255)

                                                  taACGGCTATGCGGCTGACCTGTCCTGTATCTGGTCGCCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTAcc                                                                                     <  1:952583/140‑1 (MQ=255)

                                                  taACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTAcc                                                                                     <  2:194146/140‑1 (MQ=255)

                                                  taACGGCTATGCGGCTGACATGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCTCCATGAAAGCAGGCGTCAGCTATGTGGATTAcc                                                                                     <  1:2311046/140‑1 (MQ=255)

                                                  taACGGCTATGCGGCCGACCTGACCGGTAACTGGTCGTCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTAcc                                                                                     <  1:1077450/140‑1 (MQ=255)

                                                    aCGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACcac                                                                                   >  2:439827/1‑140 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGa                                                                                          >  1:1373950/1‑129 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGa                                                                                          <  2:1373950/129‑1 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATcc                                                                               >  1:1419185/1‑140 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATcc                                                                               >  2:2176563/1‑140 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATcc                                                                               >  1:2171647/1‑140 (MQ=255)

                                                        cTATGCGGCTGACCTGACCCGTACCTGGGCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTTGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATcc                                                                               >  2:1421955/1‑140 (MQ=255)

                                                         tATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCA‑GCGTCAGCTATGTAGATTACCACATCCAg                                                                             >  1:752949/1‑140 (MQ=255)

                                                          aTGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACTATGAAAGAAGGCGTCACCTATCTGGATTACCACATCCAg                                                                             >  2:1012457/1‑140 (MQ=255)

                                                          aTGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAg                                                                             >  2:2416531/1‑140 (MQ=255)

                                                           tGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCTGGCGTCAGGTATGAGGATTACCACATCCAGt                                                                            >  2:956177/1‑140 (MQ=255)

                                                           tGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGt                                                                            >  1:1564240/1‑140 (MQ=255)

                                                           tGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGt                                                                            >  2:2206108/1‑140 (MQ=255)

                                                           tGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGt                                                                            >  1:2190126/1‑140 (MQ=255)

                                                             cGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATGGCCACCATCAAAGCAGCCGTCAGCTATGTGGATTACCACATCCAGTTc                                                                          >  2:1077450/1‑140 (MQ=255)

                                                               gcggacctgaccCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCa                                                                        >  2:1587923/4‑140 (MQ=255)

                                                                     ctgaccCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAgcg                                                                  <  2:2100644/140‑1 (MQ=255)

                                                                       gaccCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCa                                                                <  1:1421955/140‑1 (MQ=255)

                                                                        gcccGTACCTGGTCGGCTAAAATTGACAACCACAATGCGAAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCAt                                                               <  2:1189777/139‑1 (MQ=255)

                                                                             tACCTGGTCGGCAAACAGTGACTACTACTACGCGCAGTTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCa                                                          <  1:1146413/140‑1 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCa                                                          >  2:302848/1‑140 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCa                                                          <  2:116087/140‑1 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCa                                                          >  1:2209787/1‑140 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCa                                                          >  1:116087/1‑140 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAGGTGGATTGCCATATGCAGTTCGATCAGCGCATCGCCa                                                          >  1:1150808/1‑140 (MQ=255)

                                                                             tACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTAAGTGGATTACCAAATGCAGTTCGATCAGCGCATCGCCa                                                          >  1:763223/1‑140 (MQ=255)

                                                                                  ggTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAActg                                                     >  2:1988520/1‑140 (MQ=255)

                                                                                  ggTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAActg                                                     >  2:114918/1‑140 (MQ=255)

                                                                                    tCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGTTTATCACGTCCAGTTCCATCAGCGCATCGCCAAActgct                                                   >  2:822215/1‑140 (MQ=255)

                                                                                       gCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCACTTCGATGAGCGCATCGCCAAAcctgtctc                                                >  1:865517/1‑133 (MQ=255)

                                                                                              gTGACAACGACTACGCGCAGCTGGTGAAGGACGTTCATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACAt                                         <  2:1409631/140‑1 (MQ=255)

                                                                                                 aCAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCaa                                      <  1:1781136/140‑1 (MQ=255)

                                                                                                 aCAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTGCCATCAGAGCAGCGACAAACTGCTGCGTAAACATCaa                                      >  1:678436/1‑140 (MQ=255)

                                                                                                   aaCGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAat                                    <  2:2190126/140‑1 (MQ=255)

                                                                                                     cGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAatca                                  <  1:2056903/140‑1 (MQ=255)

                                                                                                     cGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAatca                                  <  1:203811/140‑1 (MQ=255)

                                                                                                       aCTACGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAatcatc                                <  1:271095/140‑1 (MQ=255)

                                                                                                       aCTACGCGCAGCTGGAGAAGGATGTTAATCATGAGCACCTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCGAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAatcatc                                <  1:675638/140‑1 (MQ=255)

                                                                                                          tcgcgCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAA‑‑‑CTGCGTAAACATCAAATCATCACCGat                          <  2:445876/139‑1 (MQ=255)

                                                                                                          aCGCGCAGCTGGTGAAGTACGTCAATGATGACCAACTGGTGCTGATCGTCACCATGAAAGCAGGTGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCAcc                             <  2:773141/140‑1 (MQ=255)

                                                                                                          aCGCGCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCAcc                             <  1:2412778/140‑1 (MQ=255)

                                                                                                           cgcgCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAatcatc                                <  1:483265/136‑1 (MQ=255)

                                                                                                           cgcgCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCg                            >  1:2340861/1‑140 (MQ=255)

                                                                                                           cgcgCAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCg                            >  1:512468/1‑140 (MQ=255)

                                                                                                             cgcAGCTGGTGAAGGGCGTTAATGATGAGCAACTGGCTCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGTATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGat                          <  1:956177/140‑1 (MQ=255)

                                                                                                             cgcAGCTGGTGAAGGACGTTAATGATGAGCAGCTGACGCAGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGat                          <  2:1051669/140‑1 (MQ=255)

                                                                                                             cgcAGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGat                          <  1:2206108/140‑1 (MQ=255)

                                                                                                                aGCTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATg                       >  2:2489126/1‑140 (MQ=255)

                                                                                                                  cTGGTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGACAAACTGCTGCGTAAACATCAAATCATCACCGATATGAg                     >  2:864060/1‑140 (MQ=255)

                                                                                                                     gTGAAGGACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTga                  <  1:2416531/140‑1 (MQ=255)

                                                                                                                      tGAAGGACATTAATGATGAGCACCTGGCGCCCAGCGCCAACATGAAAGTAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTgaa                 <  2:1140394/140‑1 (MQ=255)

                                                                                                                          ggACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAGAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCTCCAAACTGATGCCTAAGCATCAAATCATCACCGATATGAGTgaagact             >  2:914532/1‑138 (MQ=255)

                                                                                                                           gACGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGc            >  1:1001992/1‑140 (MQ=255)

                                                                                                                            aCGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCg           <  2:1887404/140‑1 (MQ=255)

                                                                                                                            aCGTTAATGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCg           >  2:13418/1‑140 (MQ=255)

                                                                                                                                 aaTGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGt      <  1:1605902/140‑1 (MQ=255)

                                                                                                                                 aaTGATGAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGt      <  1:611894/140‑1 (MQ=255)

                                                                                                                                  atgatgAGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTc     <  1:864060/140‑1 (MQ=255)

                                                                                                                                     atgaGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGaa  >  1:18277/1‑140 (MQ=255)

                                                                                                                                     atgaGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGaa  <  1:2489126/140‑1 (MQ=255)

                                                                                                                                     atgaGCAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTACGTAAACACCAAATCATCACCGATAAGAGTCAAGAAGCGATGGTCGaa  >  2:837782/1‑140 (MQ=255)

CAGGCACCGGAAGAGATGCGCAGCTTCCTGCTGGATGCCGGGGCAGAATATAACGGCTATGCGGCTGACCTGACCCGTACCTGGTCGGCAAAAAGTGACAACGACTACGCGCAGCTGGTGAAGGACGTTAATGATGAACAACTGGCGCTGATCGCCACCATGAAAGCAGGCGTCAGCTATGTGGATTACCACATCCAGTTCCATCAGCGCATCGCCAAACTGCTGCGTAAACATCAAATCATCACCGATATGAGTGAAGAAGCGATGGTCGAA  >  NC_000913/4031863‑4032135

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`