BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,053,014	A→G	M324V (ATG→GTG)	hemN →	coproporphyrinogen III dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,053,014	0	A	G	100.0%	527.4 / NA	146	M324V (ATG→GTG)	hemN	coproporphyrinogen III dehydrogenase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (78/68); total (78/68)

Only 100 of 151 total aligned reads displayed.

ATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGTGATGACTGTAT  >  NC_000913/4052881‑4053150

         tCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCa                                                                                                                           <  1:2345276/140‑1 (MQ=255)

               tATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCa                                                                                                                     >  1:1921429/1‑140 (MQ=255)

                     tATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGTGCGATACCGATCTGCTCGGGGTGGGGGTTTCCGCCATCAGCATGATTg                                                                                                               >  2:743572/1‑140 (MQ=255)

                      aTGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCATGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTgg                                                                                                              <  2:1308599/140‑1 (MQ=255)

                       tGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCa                                                                                                                     <  2:239526/132‑1 (MQ=255)

                       tGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGc                                                                                                             >  1:2356374/1‑140 (MQ=255)

                       tGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGc                                                                                                             >  2:2401017/1‑140 (MQ=255)

                       tGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGc                                                                                                             >  2:539441/1‑140 (MQ=255)

                         gATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATc                                                                                                                         >  2:2238173/1‑126 (MQ=255)

                         gATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGa                                                                                                           <  1:1467541/140‑1 (MQ=255)

                              cTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCt                                                                                                      <  1:1439536/140‑1 (MQ=255)

                              cTTTGCCCGTCCGGATGACGAGCTGACGGTGGCCCAGAGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCt                                                                                                      <  1:796430/140‑1 (MQ=255)

                               tttGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTa                                                                                                     >  1:1878575/1‑140 (MQ=255)

                                 tGCCCGTCCGGATGACTAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcg                                                                                                   >  2:1888202/1‑140 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  1:306865/140‑1 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  1:1679896/140‑1 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  1:1699637/140‑1 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 >  1:31797/1‑140 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  2:1850633/140‑1 (MQ=255)

                                   cccGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTAGGCGTATAGGCCATCAGCATGAGTGGCGACTGCTAcgcg                                                                                                 >  2:1182387/1‑140 (MQ=255)

                                   cccGTCCGGATGACGAGATGGCCGTGGCCCAGCGTAAAGCCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  2:1056482/140‑1 (MQ=255)

                                       tCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCACAATCAGCATGATTGGGGACTGCTACGCGCAGa                                                                                             >  2:1129910/1‑140 (MQ=255)

                                        ccGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGaa                                                                                            >  1:1628940/1‑140 (MQ=255)

                                        ccGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGaa                                                                                            <  1:1129910/140‑1 (MQ=255)

                                        ccGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGaa                                                                                            >  1:213577/1‑140 (MQ=255)

                                            aTGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAg                                                                                        <  1:403661/140‑1 (MQ=255)

                                             tGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGa                                                                                       <  2:1260267/140‑1 (MQ=255)

                                             tGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGa                                                                                       >  1:1266332/1‑140 (MQ=255)

                                              gACGAGCTGGCGGTGGCCCAGCGTGAAGGAGTGCTGCTTCGTAACTTCCAGGGCTACACCTCTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGaa                                                                                      <  1:1082639/140‑1 (MQ=255)

                                               aCGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGaaa                                                                                     >  1:1929168/1‑140 (MQ=255)

                                                 gAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 <  1:2035537/126‑1 (MQ=255)

                                                 gAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTAcgcg                                                                                                 >  2:2035537/1‑126 (MQ=255)

                                                 gAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCAACAGGATGGTTGGCGACGGCTAAGCGCAGAACCAGAAaga                                                                                   >  2:712310/1‑140 (MQ=255)

                                                 gAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGCTTCCGCCAACAGCACGGTTGGCGACAGCTACTCCCCGAACCAGaactg                                                                                   >  2:1140187/1‑137 (MQ=255)

                                                   gCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGt                                                                                 <  2:1266332/140‑1 (MQ=255)

                                                   gCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGACGAGTGCTACGCGTAGAACCAGAAAGAGt                                                                                 >  1:798043/1‑140 (MQ=255)

                                                   gCTGGCGGTGGCCCAGCGTGAAAGCGTGCTGTATCGTAATTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGt                                                                                 <  2:976874/140‑1 (MQ=255)

                                                   gCTGGCGGTGGCCCAGCCTGAACGCGTGCTGCATCCTAAATTCCAGGGTTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGt                                                                                 <  2:695357/140‑1 (MQ=255)

                                                     tGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTg                                                                               <  1:2438681/140‑1 (MQ=255)

                                                     tGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTg                                                                               >  1:1533444/1‑140 (MQ=255)

                                                      ggCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGa                                                                              >  2:1612515/1‑140 (MQ=255)

                                                      ggCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGa                                                                              >  2:577487/1‑140 (MQ=255)

                                                      ggCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGa                                                                              <  1:2082467/140‑1 (MQ=255)

                                                      ggCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGa                                                                              <  2:213577/140‑1 (MQ=255)

                                                       gCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGaa                                                                             <  1:2206446/140‑1 (MQ=255)

                                                         ggTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGc                                                                           <  1:2401017/140‑1 (MQ=255)

                                                           tGGCCCAGCGTGAAGTCGTGCTGCAGCGTTACTTCCATGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAg                                                                         <  1:1159082/140‑1 (MQ=255)

                                                             gCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTa                                                                       >  2:400731/1‑140 (MQ=255)

                                                                 aGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTCCTACGCGGAGATCCAGAAAGAGTTGAAGCAGTACTAt                                                                   >  1:1027388/1‑140 (MQ=255)

                                                                 aGCGTGAAGGCGTGCTGCATCGTAACTCCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTAt                                                                   >  2:2156482/1‑140 (MQ=255)

                                                                      gAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCa                                                              <  2:339985/140‑1 (MQ=255)

                                                                      gAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCa                                                              <  1:1249839/140‑1 (MQ=255)

                                                                      gAAGGCGTGCTGCATCGTAACTCCCTGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCa                                                              <  1:1182387/140‑1 (MQ=255)

                                                                       aaGGCGTGCTGCATCGTAACTTCCAGGGCTTCACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTg                                                                               >  2:1530555/1‑122 (MQ=255)

                                                                       aaGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCaa                                                             >  1:479451/1‑140 (MQ=255)

                                                                             tgctgcATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTAc                                                                      >  1:157483/1‑125 (MQ=255)

                                                                             tgctgcATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGAt                                                       >  1:2219898/1‑140 (MQ=255)

                                                                                  cATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGTAAGAGTTCAAGAAGGACTATCCGCAAGTGGATGAACa                                                  >  1:1020409/1‑140 (MQ=255)

                                                                                   aTCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTgg                                                         <  1:1386457/132‑1 (MQ=255)

                                                                                   aTCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTgg                                                         >  2:1386457/1‑132 (MQ=255)

                                                                                    tCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAg                                                >  1:849985/1‑140 (MQ=255)

                                                                                     cGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAgg                                               <  2:479451/140‑1 (MQ=255)

                                                                                       tAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCa                                             <  1:2295418/140‑1 (MQ=255)

                                                                                            tCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCACAAAGAGTTGAAGAAGTGCTATCAGCCAGTGGATGAACAAGGCAATgcg                                        >  2:822521/1‑140 (MQ=255)

                                                                                             ccAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATgcgc                                       <  1:2156482/140‑1 (MQ=255)

                                                                                               aGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCtg                                     >  1:89045/1‑140 (MQ=255)

                                                                                                 ggCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCtgtg                                   <  2:1921429/140‑1 (MQ=255)

                                                                                                 ggCTACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCtgtg                                   <  1:233598/140‑1 (MQ=255)

                                                                                                    tACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCg                                <  1:2231116/140‑1 (MQ=255)

                                                                                                    tACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCg                                <  1:410873/140‑1 (MQ=255)

                                                                                                    tACACCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCg                                <  1:1612515/140‑1 (MQ=255)

                                                                                                     acacCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGt                               >  1:494608/1‑140 (MQ=255)

                                                                                                     acacCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGt                               >  2:2450936/1‑140 (MQ=255)

                                                                                                     acacCACTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGAAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGt                               >  1:1528021/1‑140 (MQ=255)

                                                                                                      caccacTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACGATCGGCAAGTGGATGAGCAAGTCAATGCGCTGTGGCGTg                              >  2:918268/1‑140 (MQ=255)

                                                                                                       accacTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTgg                             <  2:1628940/140‑1 (MQ=255)

                                                                                                           cTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCGAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTAtt                         >  1:742256/1‑140 (MQ=255)

                                                                                                           cTCAGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTAtt                         >  2:106920/1‑140 (MQ=255)

                                                                                                              aGGGCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTgcg                      >  1:2265870/1‑140 (MQ=255)

                                                                                                                ggCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGGTGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCt                    >  1:2193380/1‑140 (MQ=255)

                                                                                                                ggCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCt                    >  1:1796641/1‑140 (MQ=255)

                                                                                                                ggCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCt                    >  1:478866/1‑140 (MQ=255)

                                                                                                                ggCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCt                    >  1:1722107/1‑140 (MQ=255)

                                                                                                                ggCGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCt                    >  2:2283480/1‑140 (MQ=255)

                                                                                                                  cGATACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTaa                  <  1:577487/140‑1 (MQ=255)

                                                                                                                    aTACCGATTGGTTTGGGGTGGGCGTTTCCGCCTTCAGCATGATTGGCGACTACTACGCGCAAAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAAcg                <  2:1528021/140‑1 (MQ=255)

                                                                                                                    aTACCGATCTGCTGGTGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAAcg                <  1:626952/140‑1 (MQ=255)

                                                                                                                     tACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTa                           <  2:2371140/128‑1 (MQ=255)

                                                                                                                     tACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTa                           >  1:2371140/1‑128 (MQ=255)

                                                                                                                     tACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAAcgc               >  2:1976181/1‑140 (MQ=255)

                                                                                                                     tACCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAAcgc               >  1:504475/1‑140 (MQ=255)

                                                                                                                      aCCGATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTCCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAAcgcg              >  2:506893/1‑140 (MQ=255)

                                                                                                                        cGATCTGCTGGGGGTGGACGTTGCCGCCATCTGCATGATCGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtg            <  2:682183/140‑1 (MQ=255)

                                                                                                                         gATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtga           <  2:2475766/140‑1 (MQ=255)

                                                                                                                         gATCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtga           <  2:2356374/140‑1 (MQ=255)

                                                                                                                          aTCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtgat          >  2:1192891/1‑140 (MQ=255)

                                                                                                                          aTCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtgat          >  2:1297737/1‑140 (MQ=255)

                                                                                                                          aTCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtgat          >  2:1767003/1‑140 (MQ=255)

                                                                                                                          aTCTGCTGGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACAAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGtgat          >  2:1382775/1‑140 (MQ=255)

                                                                                                                             tgctgGGGGTGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGTGATGAc       <  1:1888202/140‑1 (MQ=255)

ATCGGGCTATCAGTTTATCGGTATGGATCACTTTGCCCGTCCGGATGACGAGCTGGCGGTGGCCCAGCGTGAAGGCGTGCTGCATCGTAACTTCCAGGGCTACACCACTCAGGGCGATACCGATCTGCTGGGGATGGGCGTTTCCGCCATCAGCATGATTGGCGACTGCTACGCGCAGAACCAGAAAGAGTTGAAGCAGTACTATCAGCAAGTGGATGAACAAGGCAATGCGCTGTGGCGTGGTATTGCGCTAACGCGTGATGACTGTAT  >  NC_000913/4052881‑4053150

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`