BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,241,484	T→C	K257K (AAA→AAG)	xylE ←	D‑xylose:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,241,484	0	T	C	99.0%	335.8 / NA	99	K257K (AAA→AAG)	xylE	D‑xylose:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (41/57); minor base G (1/0); total (42/57)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 101 total aligned reads displayed.

CTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAAC  >  NC_000913/4241348‑4241619

ttaccGGCGCGTAGTTCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTGCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  2:115236/137‑1 (MQ=255)

cTTCCGGCGCGTAGTCCAGCACCACGTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:955000/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACCTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:922722/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:520976/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:496382/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:1279374/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  2:1643150/140‑1 (MQ=255)

cTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTa                                                                                                                                      <  1:1765026/140‑1 (MQ=255)

         cgTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATAATGCAGGGAATGCTTAATTTCCTGt                                                                                                                             >  1:1217128/1‑140 (MQ=255)

           tagtaCAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTAc                                                                                                                           <  2:1310007/140‑1 (MQ=255)

               aCAGCACCACATTGATGGCGACGAATTGCTGCAAGCTGGTGAGCATTAAGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGcc                                                                                                                       <  2:774224/140‑1 (MQ=255)

                 aGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTg                                                                                                                     <  1:180922/140‑1 (MQ=255)

                   caccacATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAg                                                                                                                   >  2:2182260/1‑140 (MQ=255)

                    accacATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGt                                                                                                                  >  1:1574685/1‑140 (MQ=255)

                      cacaTTGATGCCGACGAATTGCGGGGAGAGGGAGAGCTTTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTg                                                                                                                <  1:961850/140‑1 (MQ=255)

                      cacaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGGGTGCTTAATTTCCTGTACTGCCTGAGTTg                                                                                                                >  1:1205661/1‑140 (MQ=255)

                      cacaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTc                                                                                                                                 <  1:1852948/123‑1 (MQ=255)

                      cacaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTc                                                                                                                                 >  2:1852948/1‑123 (MQ=255)

                      cacaTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTg                                                                                                                >  2:166933/1‑140 (MQ=255)

                       acaTTGATGCCGACAAATTGCTGGAAGATTGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGc                                                                                                               <  1:1075394/140‑1 (MQ=255)

                        caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATTCAGGGAGGGCTTAAATTCCTGGACTGCCTGAGTTGCa                                                                                                              >  1:771128/1‑140 (MQ=255)

                        caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATGCAGGGAGGGCTCAACTTCCTGAACTGCCTGAGTTGCa                                                                                                              >  1:795419/1‑140 (MQ=255)

                        caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCa                                                                                                              >  1:1416774/1‑140 (MQ=255)

                        caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCa                                                                                                              >  1:1431420/1‑140 (MQ=255)

                        caTTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCa                                                                                                              >  1:391518/1‑140 (MQ=255)

                          ttGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCGGGGAGTGGTTAGTTGCCTGTAGTGCCTGAGTTGCAAg                                                                                                            >  1:748710/1‑140 (MQ=255)

                             aTGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCgt                                                                                                         >  2:591867/1‑140 (MQ=255)

                               gCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCgtgt                                                                                                       <  2:2135973/140‑1 (MQ=255)

                                ccGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGtt                                                                                                      >  1:300141/1‑140 (MQ=255)

                                   aCAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGcc                                                                                                   >  2:1411713/1‑140 (MQ=255)

                                   aCAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGcc                                                                                                   >  1:324610/1‑140 (MQ=255)

                                   aCAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCGTAATTTCATGTGCTACCTGAGCTGCAAGCGTGTTGcc                                                                                                   >  1:1154358/1‑140 (MQ=255)

                                     aaaTTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGccaa                                                                                                 <  1:166933/140‑3 (MQ=255)

                                     aaaTTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCa                                                                                                 <  2:1274817/140‑1 (MQ=255)

                                             ggAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTg                                                                                         <  2:1574685/140‑1 (MQ=255)

                                              gAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgc                                                                                        <  2:562638/140‑1 (MQ=255)

                                               aaGATGGAGAGCATTGCGCCGACTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgcg                                                                                       <  1:1146020/140‑1 (MQ=255)

                                               aaGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGTCGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTGCTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTgcg                                                                                       <  2:1774696/140‑1 (MQ=255)

                                                   tGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAgg                                                                                   >  2:402752/1‑140 (MQ=255)

                                                    ggAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGa                                                                                  <  1:1924487/140‑1 (MQ=255)

                                                    ggAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGa                                                                                  <  2:2324847/140‑1 (MQ=255)

                                                        agCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                                                              >  1:912269/1‑140 (MQ=255)

                                                        agCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATAcc                                                                              >  1:518270/1‑140 (MQ=255)

                                                          cATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCtt                                                                            >  2:451430/1‑140 (MQ=255)

                                                            ttACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTcc                                                                          <  1:2182260/140‑1 (MQ=255)

                                                             tACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCg                                                                         >  1:1487347/1‑140 (MQ=255)

                                                              aCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATGCCTTCCGc                                                                        <  2:2281153/140‑1 (MQ=255)

                                                              aCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGc                                                                        <  1:1411713/140‑1 (MQ=255)

                                                              aCGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGc                                                                        <  2:14152/140‑1 (MQ=255)

                                                               cGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCt                                                                             >  1:1790596/1‑134 (MQ=255)

                                                               cGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCt                                                                             <  2:1790596/134‑1 (MQ=255)

                                                                 ccGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTg                                                                     <  2:1657899/140‑1 (MQ=255)

                                                                    aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATTATTTTGGGCAGGATACCTTCCGCCTGTTc                                                                  >  1:924021/1‑140 (MQ=255)

                                                                    aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTc                                                                  >  1:66675/1‑140 (MQ=255)

                                                                    aTTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTc                                                                  >  1:1944663/1‑140 (MQ=255)

                                                                         aaTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgct                                                             <  2:2057197/140‑1 (MQ=255)

                                                                          aTCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTcttgctt                                                            >  1:480435/1‑140 (MQ=255)

                                                                            cacgcccatgccAAACATCAGCTGACGACCATCCGTTTTTCGGCCATGTTCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCttgcttgc                                                          <  2:1154358/140‑1 (MQ=255)

                                                                              cgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                                                        >  1:1637197/1‑140 (MQ=255)

                                                                              cgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                                                        >  1:1765863/1‑140 (MQ=255)

                                                                              cgcccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcg                                                        >  1:74688/1‑140 (MQ=255)

                                                                                ccctcgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATTCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATTCCTTCCGCCTGTTCTTGCTTGCcgcg                                                      <  2:236779/140‑1 (MQ=255)

                                                                                cccacgccAAACATCGGCAGACGACCACCGGTTTTGCTGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                                                      <  1:1071597/140‑1 (MQ=255)

                                                                                cccacgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCcgcg                                                      >  1:2305378/1‑140 (MQ=255)

                                                                                   acgccAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGa                                                   >  1:1449814/1‑140 (MQ=255)

                                                                                            aTCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATcagcca                                          >  1:147312/1‑140 (MQ=255)

                                                                                               agcagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCACAGGATACCTTCGGCCGGTTCTTGCCTGCCTCGCGACATCAGCCAGCg                                       >  2:671933/1‑140 (MQ=255)

                                                                                                 cagACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAg                                     <  2:1448976/140‑1 (MQ=255)

                                                                                                 cagACGACCACCGGTTTGGCGGCCATGATCCTGGGGGTGCTTAATTTCTTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAg                                     <  2:748710/140‑1 (MQ=255)

                                                                                                     cgacCACCGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACt                                 >  2:1853331/1‑140 (MQ=255)

                                                                                                          accGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTg                            <  1:402752/140‑1 (MQ=255)

                                                                                                          accGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTg                            <  2:518270/140‑1 (MQ=255)

                                                                                                           ccGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTgg                           <  2:1803490/140‑1 (MQ=255)

                                                                                                           ccGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTgg                           >  1:1803490/1‑140 (MQ=255)

                                                                                                           ccGGTTTTGCGGCCACGATCCAGGGAGTGTTTAATTTACTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTgg                           <  1:1055622/140‑1 (MQ=255)

                                                                                                            cGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGc                          >  1:614622/1‑140 (MQ=255)

                                                                                                            cGGTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGc                          <  1:222855/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTTCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  2:480435/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  2:324610/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  2:2206613/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  1:111440/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  1:368110/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  2:1637197/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  1:175741/140‑1 (MQ=255)

                                                                                                             ggTTTTGCGGCCATGAGCCAGGGGGTGCTTAATTTCCTGTACTGCCTGAGTTGCTAGCGCGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCa                         <  2:1895953/140‑1 (MQ=255)

                                                                                                              gTTTTGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCAc                        >  2:1826283/1‑140 (MQ=255)

                                                                                                                  tGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTg                            <  1:1821953/132‑1 (MQ=255)

                                                                                                                  tGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTg                            >  2:1821953/1‑132 (MQ=255)

                                                                                                                  tGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGta                    >  2:1376776/1‑140 (MQ=255)

                                                                                                                  tGCGGCCATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGATCTTGCTTGCCGCGCGGCATCAGCCAGCGATGACTTTCTGGCACGGta                    >  2:1037294/1‑140 (MQ=255)

                                                                                                                    cGGCCATGATCCAGGGCATGCTTAATTTCCAGTGCGGCCTGAGTTGCATGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGtata                  <  2:1205661/140‑1 (MQ=255)

                                                                                                                       ccATGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcag               <  1:591867/140‑1 (MQ=255)

                                                                                                                         aTGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagca             <  2:300141/140‑1 (MQ=255)

                                                                                                                         aTGATCCAGGGAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAcagca             <  1:451430/140‑1 (MQ=255)

                                                                                                                            aTCCAGGGAGTGCTTCATTTCCAGTCCTGCCTGAGTTCCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAgcagca          <  1:671933/140‑1 (MQ=255)

                                                                                                                                 gggAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAg     <  2:1487347/140‑1 (MQ=255)

                                                                                                                                   gAGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGaa   <  2:1944663/140‑1 (MQ=255)

                                                                                                                                    aGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATCCCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAAc  <  2:2277637/140‑1 (MQ=255)

                                                                                                                                    aGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAca                >  1:2269536/1‑126 (MQ=255)

                                                                                                                                    aGTGCTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATAca                <  2:2269536/126‑1 (MQ=255)

CTTCCGGCGCGTAGTACAGCACCACATTGATGCCGACAAATTGCTGGAAGATGGAGAGCATTACGCCGATTACAATCACGCCCACGCCAAACATCAGCAGACGACCACCGGTTTTGCGGCCATGATCCAGGGAGTGTTTAATTTCCTGTACTGCCTGAGTTGCAAGCGTGTTGCCCATAATTTTGCGCAGGATACCTTCCGCCTGTTCTTGCTTGCCGCGCGACATCAGCCAGCGAGGACTTTCTGGCACGGTATACAGCAGCATTAAGAAC  >  NC_000913/4241348‑4241619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`