Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	524,518	G→T	A420S (GCG→TCG)	rhsD →	protein RhsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	524,518	0	G	T	98.9%	300.8 / NA	91	A420S (GCG→TCG)	rhsD	protein RhsD
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base T (51/39);  total (52/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AACTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGGCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATCACCACACCGGAC  >  NC_000913/524381‑524652                                                                                                                                          |                                                                                                                                       aaCTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCg                                                                                                                                      >  1:2003622/1‑140 (MQ=255)  aCTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGAGAAAAAAGAACTGTCgg                                                                                                                                     >  1:2410246/1‑140 (MQ=255)   cTGAACCCGGCAGGGTTTAGCTACCGCTATCTTTAAGAGTAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGa                                                                                                                                    <  2:879599/140‑1 (MQ=255)   cTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGa                                                                                                                                    <  2:1907319/140‑1 (MQ=255)   cTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGa                                                                                                                                    >  1:1243631/1‑140 (MQ=255)             cAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAACCGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTc                                                                                                                          <  2:1646252/140‑1 (MQ=255)             cAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTc                                                                                                                          >  1:1728229/1‑140 (MQ=255)                            gCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAATAGAACTGGCGGTCGGCAGCGTTACGCGCAGCGGGTAt                                                                                                           >  1:837191/1‑140 (MQ=255)                                tCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGAcg                                                                                                       >  1:325986/1‑140 (MQ=255)                                    tATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGc                                                                                                   >  1:316346/1‑140 (MQ=255)                                     aTGAGCATAACCGCCACACCCTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCa                                                                                                  >  2:2351639/1‑140 (MQ=25)                                        aGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGAGAAAAAAGAACTGTCGGCCGGCAGCATCATGCGCAGCGGGGATGACGCGGCAGGa                                                                                               >  1:937851/1‑140 (MQ=37)                                          cAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAg                                                                                             >  1:1077542/1‑140 (MQ=255)                                          cAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAg                                                                                             >  1:1239078/1‑140 (MQ=255)                                          cAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAg                                                                                             >  1:1925704/1‑140 (MQ=255)                                            ggACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGc                                                                                           >  2:2371926/1‑140 (MQ=255)                                            ggACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGc                                                                                           >  1:2357081/1‑140 (MQ=255)                                            ggACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGc                                                                                           >  2:332228/1‑140 (MQ=255)                                                 gCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACg                                                                                      <  2:1784782/140‑1 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCa                                                                                        >  1:547501/1‑137 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCa                                                                                        <  2:547501/137‑1 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACgg                                                                                     >  1:2475300/1‑140 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACgg                                                                                     >  1:108327/1‑140 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACgg                                                                                     >  1:1510929/1‑140 (MQ=255)                                                  cATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACgg                                                                                     >  1:1735295/1‑140 (MQ=255)                                                       ccgtcaccgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAGAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAg                                                                                >  1:2478418/1‑140 (MQ=255)                                                       ccgtcaccgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAg                                                                                >  1:2280412/1‑140 (MQ=255)                                                         gtcaccgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAgac                                                                              >  1:503735/1‑140 (MQ=255)                                                          tcaccgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAgacg                                                                             <  2:2357081/140‑1 (MQ=255)                                                            accgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAgacgga                                                                           >  1:2107948/1‑140 (MQ=255)                                                              cgACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGAGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAgacggacg                                                                         <  2:2410246/140‑1 (MQ=255)                                                               gACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGAcgc                                                                        >  1:1333999/1‑140 (MQ=255)                                                                 cAGCCTGAACCGGCGTGCGGTGCTGCATACATAAGCCGGTGCCGGGCTCAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcgg                                                                      <  2:753957/140‑1 (MQ=37)                                                                 cAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcgg                                                                      >  1:1526514/1‑140 (MQ=255)                                                                 cAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcgg                                                                      >  1:1602417/1‑140 (MQ=255)                                                                 cAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcgg                                                                      >  1:628452/1‑140 (MQ=255)                                                                   gCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcggcg                                                                    <  2:1728229/140‑1 (MQ=255)                                                                   gCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcggcg                                                                    <  2:302895/140‑1 (MQ=255)                                                                   gCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACgcggcg                                                                    <  2:1909915/140‑1 (MQ=255)                                                                    ccTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGcggcgg                                                                   >  1:1654876/1‑140 (MQ=255)                                                                     cTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCggg                                                                  <  2:1243631/140‑1 (MQ=255)                                                                        aaCCGGCGTGAGGTGCTGCATAGAGAAGGCGGGGCCGGGTTGAAACGGATGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACg                                                               <  2:1048323/140‑1 (MQ=255)                                                                        aaCCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACg                                                               <  2:108327/140‑1 (MQ=255)                                                                          ccGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACgga                                                             <  2:1333999/140‑1 (MQ=255)                                                                          ccGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACgga                                                             <  2:17410/140‑1 (MQ=255)                                                                             gCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACggagga                                                          <  2:2162738/140‑1 (MQ=255)                                                                              cGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGAc                                                         <  2:1526514/140‑1 (MQ=255)                                                                              cGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGAc                                                         >  2:415359/1‑140 (MQ=255)                                                                              cGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGAc                                                         <  2:1735295/140‑1 (MQ=255)                                                                              cGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGAc                                                         <  2:2280412/140‑1 (MQ=255)                                                                                  aGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGGAGAGGGGCGCGGCGGGACGGAGGACagag                                                     >  1:1206599/1‑140 (MQ=255)                                                                                  aGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACagag                                                     >  2:1884220/1‑140 (MQ=255)                                                                                   ggTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGt                                                    <  2:147924/140‑1 (MQ=255)                                                                                    gTGCTGCATACAGAAGGCGGGGCCGGGCTGACACGTGTGCTGAAAAAATAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTa                                                   <  2:1077542/140‑1 (MQ=255)                                                                                       ctgcATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACgg                                                <  2:1510929/140‑1 (MQ=255)                                                                                       ctgcATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACgg                                                <  2:2107948/140‑1 (MQ=255)                                                                                       ctgcATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACgg                                                >  1:2262505/1‑140 (MQ=255)                                                                                       ctgcATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACCGACTCGGCGGGACGGAGGACAGAGTACgg                                                >  1:895588/1‑140 (MQ=255)                                                                                               aGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAAtg                                        <  2:2262505/140‑1 (MQ=255)                                                                                               aGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAAtg                                        >  1:2074151/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCGCGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATgtggtg                                   >  1:176009/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTa                                                   >  1:1721755/1‑124 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTa                                                   <  2:1721755/124‑1 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATgtggtg                                   >  1:232573/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATgtggtg                                   >  2:1336950/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGGACGGTCTGAATgtggtg                                   >  2:1758364/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGAAGGAGGACAGAGTACGGTCTGAATgtggtg                                   >  1:1117504/1‑140 (MQ=255)                                                                                                    gCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCGCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATgtggtg                                   <  2:1791602/140‑1 (MQ=255)                                                                                                      ggggCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTc                                 <  2:232573/140‑1 (MQ=255)                                                                                                        ggCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCg                               <  2:1602417/140‑1 (MQ=255)                                                                                                        ggCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCg                               <  2:628452/140‑1 (MQ=255)                                                                                                        ggCCGGGCTGAAACGGGCGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGAACGGTCTGAATGTGCGGTCCg                               >  2:1917070/1‑140 (MQ=255)                                                                                                         gCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCGCGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCgg                              <  2:176009/140‑1 (MQ=255)                                                                                                         gCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCgg                              >  1:164229/1‑140 (MQ=255)                                                                                                         gCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGAACAAAGAACGGTCCGAATGTGGTGTCCgg                              >  1:728570/1‑140 (MQ=255)                                                                                                           cGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGCCGGAGGACAGAGGAAGGTCTGAATGTGGTGTCCGGCg                            >  1:817116/1‑140 (MQ=255)                                                                                                           cGGGCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCg                            <  2:325986/140‑1 (MQ=255)                                                                                                            gggCTGAAACGGGTGGTTAAAACAGAACTGTTGGATGGCAGCGTCACGGGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGa                           <  2:817116/140‑1 (MQ=255)                                                                                                              gCTGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGata                         >  1:274326/1‑140 (MQ=255)                                                                                                                tGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATc                       <  2:2003622/140‑1 (MQ=255)                                                                                                                tGAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATc                       <  2:1925704/140‑1 (MQ=255)                                                                                                                 gAAACGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCa                      <  2:2057434/140‑1 (MQ=255)                                                                                                                     cGGGTGGTGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGa                  <  1:2351639/140‑1 (MQ=255)                                                                                                                       ggtggtGAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACa                >  1:1910912/1‑140 (MQ=255)                                                                                                                        gtggtgAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACAt               >  1:175369/1‑140 (MQ=255)                                                                                                                        gtggtgAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACAt               >  2:391990/1‑140 (MQ=255)                                                                                                                           gtgAAAAAAGAACTGTCTGACGTCAGCGTCATGCGAAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGTGATATCACGGACATcac            <  2:847082/140‑1 (MQ=255)                                                                                                                            tgAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATcacc           >  1:1664568/1‑140 (MQ=255)                                                                                                                            tgAAAAAAGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATcacc           >  1:2157269/1‑140 (MQ=255)                                                                                                                                aaaaGAACTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATCGCCacac       >  1:1692145/1‑140 (MQ=255)                                                                                                                                    gAACTGTCGGACGGCAGCGTCAAGCGCAGCGTGTACGACTCGGCAGGATGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATCACCACACCGGa   <  2:728570/140‑1 (MQ=255)                                                                                                                                     aaCTGTCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATCACCACACCGGAc  >  1:1515923/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       AACTGAACCCGGCAGGGTTAAGCTACCGCTATCTTTATGAGCAGGACCGCATCACCGTCACCGACAGCCTGAACCGGCGTGAGGTGCTGCATACAGAAGGCGGGGCCGGGCTGAAACGGGTGGTGAAAAAAGAACTGGCGGACGGCAGCGTCACGCGCAGCGGGTATGACGCGGCAGGAAGGCTCACGGCGCAGACGGACGCGGCGGGACGGAGGACAGAGTACGGTCTGAATGTGGTGTCCGGCGATATCACGGACATCACCACACCGGAC  >  NC_000913/524381‑524652

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑