Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,468,257 G→A S211S (TCG→TCA)  mgtA → Mg(2(+)) importing P‑type ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,468,2570GA98.0% 499.9 / NA 150S211S (TCG→TCA) mgtAMg(2(+)) importing P‑type ATPase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (75/72);  minor base C (2/1);  total (77/73)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 152 total aligned reads displayed.

AAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCGTTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATT  >  NC_000913/4468120‑4468392
                                                                                                                                         |                                                                                                                                       
aaaGGCGAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCCTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCAtt                                                                                                                                       <  2:367466/140‑1 (MQ=255)
  aGGCGAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTaa                                                                                                                                     >  1:1335693/1‑140 (MQ=255)
  aGGCGAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATATACGTATCGTGCGGGCACGGGATCTTTTCGTCGCTCAGGCGTCATTaa                                                                                                                                     >  2:791283/1‑140 (MQ=255)
      gAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTaa                                                                                                                                     <  1:48078/136‑1 (MQ=255)
      gAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTaa                                                                                                                                     >  2:48078/1‑136 (MQ=255)
       aaaaTGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGt                                                                                                                                <  2:165450/140‑1 (MQ=255)
       aaaaTGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGt                                                                                                                                <  2:1335693/140‑1 (MQ=255)
       aaaaTGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGt                                                                                                                                <  2:1326573/140‑1 (MQ=255)
       aaaaTGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGt                                                                                                                                <  1:472938/140‑1 (MQ=255)
       aaaaTGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGt                                                                                                                                <  2:2356213/140‑1 (MQ=255)
              ctggctggAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGtctc                                                                                                                         >  2:192973/1‑140 (MQ=255)
               tggctggAGATCCCGTTCGACCCGCGGGTCCCCGGCGTTATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTctct                                                                                                                        <  1:756506/140‑1 (MQ=255)
               tggctggAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTctct                                                                                                                        <  2:186567/140‑1 (MQ=255)
                   tggAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGccc                                                                                                                    <  1:1557203/140‑1 (MQ=255)
                   tggAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGccc                                                                                                                    <  1:1997966/140‑1 (MQ=255)
                   tggAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGccc                                                                                                                    <  1:1906030/140‑1 (MQ=255)
                                cGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCg                                                                                                       >  1:1643910/1‑140 (MQ=255)
                                 gACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGc                                                                                                      <  1:1586928/140‑1 (MQ=255)
                                 gACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGc                                                                                                      >  2:1395076/1‑140 (MQ=255)
                                  aCCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCTTCACTAAACGGTGAGTATCTGGCCGTAGAAAAAGCCGCt                                                                                                     >  2:647625/1‑140 (MQ=255)
                                       cTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCAc                                                                                                >  2:1277266/1‑140 (MQ=255)
                                            gCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGcc                                                                                           <  1:471987/140‑1 (MQ=255)
                                                 gCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCg                                                                                      <  1:1395076/140‑1 (MQ=255)
                                                 gCGATATTATAAAACTGCCGGCCGGAGATATTATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCg                                                                                      <  2:856857/140‑1 (MQ=255)
                                                 cagATATTATAAAACTGGCGGCAGGAGATATGATCGCGGCAGATTTACCTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCg                                                                                      <  2:824066/138‑1 (MQ=255)
                                                  cGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCgg                                                                                     <  2:516275/140‑1 (MQ=255)
                                                   gATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGa                                                                                    <  2:1563928/140‑1 (MQ=255)
                                                   gATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCAGTAGAAAAAGCCGCTACCACTCGCCAGCCGGa                                                                                    <  2:1347831/140‑1 (MQ=255)
                                                    atatTATAAAACTGGCGGCGGGAGACATGCTCCCGGCCGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAg                                                                                   <  1:647625/140‑1 (MQ=255)
                                                      attatAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGca                                                                                 <  2:2482909/140‑1 (MQ=255)
                                                               cttgcggctGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATcc                                                                        >  1:1523289/4‑140 (MQ=255)
                                                               cTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATcc                                                                        >  1:2299558/1‑140 (MQ=255)
                                                               cTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCAGGAGCACAGCAATcc                                                                        >  1:1204642/1‑140 (MQ=255)
                                                                tGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCg                                                                       <  1:1993623/140‑1 (MQ=255)
                                                                 ggcggcGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGGTCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGc                                                                      >  2:1969752/1‑140 (MQ=255)
                                                                 ggcggcGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCg                                                                                      >  1:1894987/1‑124 (MQ=255)
                                                                 ggcggcGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAg                                                                              >  1:1813726/1‑132 (MQ=255)
                                                                 ggcggcGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAg                                                                              <  2:1813726/132‑1 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCGCGTAGAAAAAGCCGGTGCCACTCGCAAGCCGAAGCACAGGAATCCGCt                                                                     >  2:1217375/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCt                                                                     >  2:1823063/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCt                                                                     >  1:392843/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCt                                                                     >  2:1550181/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCt                                                                     >  1:503575/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCt                                                                     >  2:22298/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTAGCTAGCCGGCGCACAGCAATCCGCt                                                                     >  1:1136461/1‑140 (MQ=255)
                                                                  gcggcgGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCACGTAGAAAGAGCGGCTTCCACTCGCCAGCCAGAGCACAGCAATCCGCt                                                                     >  2:724442/1‑140 (MQ=255)
                                                                            atatGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGac                                                           <  2:2468633/140‑1 (MQ=255)
                                                                            atatGATCCCGGCAGATTTACGGATTTTGCAGGCGCGCGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGac                                                           <  1:1019795/140‑1 (MQ=255)
                                                                                  tCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGaca                                                          >  1:311255/1‑135 (MQ=255)
                                                                                  tCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGaca                                                          <  2:311255/135‑1 (MQ=255)
                                                                                  tCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCtg                                                     >  2:2016917/1‑140 (MQ=255)
                                                                                   cccGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCATCCGGAGCACAGTAATCCGCTGCAGTGCGACACGCtgt                                                    >  1:1138791/1‑140 (MQ=255)
                                                                                   cccGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGa                                                            >  2:172549/1‑132 (MQ=255)
                                                                                   cccGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGa                                                            <  1:172549/132‑1 (MQ=255)
                                                                                    ccGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCtgtg                                                   >  2:488346/1‑140 (MQ=255)
                                                                                     cGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTgtgt                                                  >  1:2191965/1‑140 (MQ=255)
                                                                                      ggCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGtt                                                 >  2:1302438/1‑140 (MQ=255)
                                                                                       gCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGttt                                                >  2:2126430/1‑140 (MQ=255)
                                                                                       gCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGttt                                                >  2:2285747/1‑140 (MQ=255)
                                                                                       gCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGttt                                                >  1:1650942/1‑140 (MQ=255)
                                                                                       gCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGttt                                                >  1:2435185/1‑140 (MQ=255)
                                                                                       gCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGttt                                                >  2:2342043/1‑140 (MQ=255)
                                                                                        cAGATTTACGTATCTCGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGtttt                                               <  1:791283/140‑1 (MQ=255)
                                                                                             ttACGTATTTTGCAGGAGCGGGTTCTGTTCGCCGCCCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATggg                                          <  2:1138791/140‑1 (MQ=255)
                                                                                             ttACGTATCTTGCAGTCGCGGGCTCCGTTCGTCGCTCGGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATggg                                          <  1:909892/140‑1 (MQ=255)
                                                                                             ttACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATggg                                          >  2:2224067/1‑140 (MQ=255)
                                                                                               tcGTATCTTGCAGGCTCGGGATCTGGTCGTTGCTCAGCCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGca                                        <  1:724442/139‑1 (MQ=255)
                                                                                               aCGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGca                                        <  1:1823063/140‑1 (MQ=255)
                                                                                                     cTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCTCTGGAGTACGACACGCTGTGTTTTATGGGCACCACCg                                  >  1:1079324/1‑140 (MQ=255)
                                                                                                     cTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCg                                  >  1:1528651/1‑140 (MQ=255)
                                                                                                       tGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCAGAGCAATCCACTGAATTTGGACACGCAGTGTGTTATGGGCACCACCgtg                                >  2:1202482/1‑140 (MQ=255)
                                                                                                       tGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCgtg                                <  1:111225/140‑1 (MQ=255)
                                                                                                         cAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCgtggt                              >  1:2134230/1‑140 (MQ=255)
                                                                                                            gcgcGGGATCTGTTCGTCGCTCGGGCGTCCTTAACCGATGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAg                           <  1:1217375/140‑1 (MQ=255)
                                                                                                            gcgcGGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAg                           <  2:1603067/140‑1 (MQ=255)
                                                                                                               cgGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcgg                        >  2:2084069/1‑140 (MQ=255)
                                                                                                               cgGGATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCAACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCgtggtg                             <  1:388817/135‑1 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCTGGCGGCATTAACCGGTGGGTCTCTGCCCGTCGAAAAAGCCGCTCCCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      <  2:1079324/140‑1 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCCGGCGACATTAACCGGGGCGTCACTGCCCGTGGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      <  2:1101700/140‑1 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGATACGCTGTGTTTAATAGGCACCACCGTGGTGAGcggca                      >  1:744501/1‑140 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      >  1:1438795/1‑140 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      <  1:1727486/140‑1 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      <  2:392843/140‑1 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCCGTGTTTTATGGGCACCACCGTGGTGAGcggca                      >  2:10450/1‑140 (MQ=255)
                                                                                                                 ggATCTGTTCGTCGCTCAGGCGCCATTAACCCGTGAGTCTCTGCCCGTCGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggca                      <  2:662684/140‑1 (MQ=255)
                                                                                                                     cTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTTTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggcacggc                  <  1:2367948/140‑1 (MQ=255)
                                                                                                                     cTGTTCGTCGCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGcggcacggc                  <  2:527265/140‑1 (MQ=255)
                                                                                                                          cgtcgCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAg             <  1:1302438/140‑1 (MQ=255)
                                                                                                                          cgtcgCTCAGGCGTCATCAACCCGTGAGTCTATGCCCGTAGAAAAAGCGGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAg             <  2:894074/140‑1 (MQ=255)
                                                                                                                            tcgCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCa           >  1:362257/1‑140 (MQ=255)
                                                                                                                            tcgCTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCa           >  2:390975/1‑140 (MQ=255)
                                                                                                                               cTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCCCCGTGGAGAGCGGCACGGCACAAGCAATg        >  1:1110990/1‑140 (MQ=255)
                                                                                                                               cTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATg        >  1:548154/1‑140 (MQ=255)
                                                                                                                               cTCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTACGGGCACCGCCGGGGTGAGCGGAACGGCACAAGCAATg        >  1:933153/1‑140 (MQ=255)
                                                                                                                                tCAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATgg       >  2:1227802/1‑140 (MQ=255)
                                                                                                                                 cAGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGt      <  2:2299558/140‑1 (MQ=255)
                                                                                                                                  aGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTg     <  2:1879820/140‑1 (MQ=255)
                                                                                                                                  aGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTg     <  1:192973/140‑1 (MQ=255)
                                                                                                                                  aGGCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAACGGCACGGCACAAGCAATGGTg     >  1:2284735/1‑140 (MQ=255)
                                                                                                                                    gCGTCATTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGAt   <  2:1724293/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
AAAGGCGAAAATGGCTGGCTGGAGATCCCGATCGACCAGCTGGTGCCCGGCGATATTATAAAACTGGCGGCGGGAGATATGATCCCGGCAGATTTACGTATCTTGCAGGCGCGGGATCTGTTCGTCGCTCAGGCGTCGTTAACCGGTGAGTCTCTGCCCGTAGAAAAAGCCGCTACCACTCGCCAGCCGGAGCACAGCAATCCGCTGGAGTGCGACACGCTGTGTTTTATGGGCACCACCGTGGTGAGCGGCACGGCACAAGCAATGGTGATT  >  NC_000913/4468120‑4468392

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑