BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,586,655	A→G	L36P (CTG→CCG)	hsdR ←	type I restriction enzyme EcoKI endonuclease component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,586,655	0	A	G	100.0%	927.1 / NA	256	L36P (CTG→CCG)	hsdR	type I restriction enzyme EcoKI endonuclease component
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (120/136); total (120/136)

Only 100 of 265 total aligned reads displayed.

CAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGCCT  >  NC_000913/4586518‑4586789

cAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGc                                                                                                                                      >  1:1643018/1‑140 (MQ=255)

 agagagGATGTTGTCGTCAACAGAGTCGACTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:785804/140‑1 (MQ=255)

 agagagGATGTTGTCGTCAACAAAGATGAGTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:1058774/140‑1 (MQ=255)

 agagagGATGTTGTCCTCAACATAGCCGAATTTGCCGCGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:686636/140‑1 (MQ=255)

 agagagGATGTTGTCATCAACATAGTCGATTTTGCCGGGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:1101659/140‑1 (MQ=255)

 agagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  2:367241/140‑1 (MQ=255)

 agagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  2:1473329/140‑1 (MQ=255)

 agagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:1691815/140‑1 (MQ=255)

  gagagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAGACATGCGCATTTTAATCGGcgt                                                                                                                                    >  2:964697/1‑140 (MQ=255)

   agagGATGTTGTCATGAACAAACGCTATTTTGCCGAGGTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcgtc                                                                                                                                   <  1:881727/140‑1 (MQ=255)

   agagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTGGCTTTGGATTCGCCAAACATAGGCATTTTAATCGGcgtc                                                                                                                                   >  2:1058774/1‑140 (MQ=255)

   agagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcgtc                                                                                                                                   >  2:1940090/1‑140 (MQ=255)

   agagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     <  1:2432254/138‑1 (MQ=255)

   agagGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTCCGCCAAACATACGCATTTTAATCGGcgtc                                                                                                                                   >  1:1000922/1‑140 (MQ=255)

     agGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcgt                                                                                                                                    <  2:2228788/137‑1 (MQ=255)

      ggATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCgtg                                                                                                                                <  2:272848/140‑1 (MQ=255)

        aTGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGcg                                                                                                                                     >  1:1759119/1‑133 (MQ=255)

        aTGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGtt                                                                                                                              >  2:1259471/1‑140 (MQ=255)

        aTGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGtt                                                                                                                              >  1:1921425/1‑140 (MQ=255)

        aTGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGtt                                                                                                                              >  1:351546/1‑140 (MQ=255)

          gttgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTgg                                                                                                                            >  2:359777/1‑140 (MQ=255)

          gttgtCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCGTCGCCAACCATCCGAATTTTACTCGGCGTCGTGTTgg                                                                                                                            >  1:996877/1‑140 (MQ=255)

              tcatcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGatc                                                                                                                        >  1:2151801/1‑140 (MQ=255)

               catcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGACTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGatca                                                                                                                       >  2:1595667/1‑140 (MQ=255)

                 tcaACAAAGGTGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGAGGCTTCGCCAAACAAACCCATTTTAATCGGATTCGTGTTTGGatcatc                                                                                                                     >  2:1176449/1‑140 (MQ=255)

                 tcaACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGatcatc                                                                                                                     >  2:1652639/1‑140 (MQ=255)

                        aGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGatcatc                                                                                                                     >  1:2227521/1‑133 (MQ=255)

                        aGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCggg                                                                                                                 <  1:491663/137‑1 (MQ=255)

                        aGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCggg                                                                                                                 >  2:491663/1‑137 (MQ=255)

                           cGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTATAAGCGGCGTCGAGTTGGGATCATCCGGGTAGTTa                                                                                                           >  2:885768/1‑140 (MQ=255)

                                       gTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGca                                                                                               >  1:1720678/1‑140 (MQ=255)

                                         tCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATATTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGcaca                                                                                             <  1:2263286/140‑1 (MQ=255)

                                            cGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGc                                                                                          <  2:2151801/140‑1 (MQ=255)

                                            cGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGc                                                                                          <  1:1951823/140‑1 (MQ=255)

                                              cAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGa                                                                                        <  1:1595667/140‑1 (MQ=255)

                                              cAGGAGATCGTGTTGATCCTCACAAGGGGGGCTGTCGAGTAACAGACCGAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGa                                                                                        <  2:700470/140‑1 (MQ=255)

                                                  agaTCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGa                                                                                        <  1:1290271/136‑1 (MQ=255)

                                                  agaTCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGc                                                                                    <  2:586386/140‑1 (MQ=255)

                                                    aTCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCAt                                                                                  >  2:8068/1‑140 (MQ=255)

                                                     tCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATa                                                                                 <  2:397218/140‑1 (MQ=255)

                                                       gtgtTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAg                                                                               >  1:1324351/1‑140 (MQ=255)

                                                           tGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGa                                                                                        <  2:1539769/127‑1 (MQ=255)

                                                           tGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGaa                                                                           >  1:1315224/1‑140 (MQ=255)

                                                           tGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGaa                                                                           >  2:2443893/1‑140 (MQ=255)

                                                                  cacaAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTg                                                                    >  1:1987012/1‑140 (MQ=255)

                                                                  cacaAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTg                                                                    <  2:2478058/140‑1 (MQ=255)

                                                                   acaAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa                                                                   >  1:185929/1‑140 (MQ=255)

                                                                   acaAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa                                                                   <  2:1670434/140‑1 (MQ=255)

                                                                   acaAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACACGCGATGGCATAAGTGAAGTCGTTGa                                                                   <  2:1694481/140‑1 (MQ=255)

                                                                   acaAGGGGGGATGTTCAGTAACAGATCAAAATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa                                                                   <  1:833041/140‑1 (MQ=255)

                                                                   acaAGGGGGGATGTTAAGTAACAGATCAATATGTTTCTCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa                                                                   <  1:913801/140‑1 (MQ=255)

                                                                     aaGGGGGGATGTTGAGTAACAGACCAAGACGTTTCGCTGTGGCTTCGCAACACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACg                                                                 <  2:2067775/140‑1 (MQ=255)

                                                                            gATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGa                                                                   >  1:584381/1‑131 (MQ=255)

                                                                            gATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCa                                                          >  1:275768/1‑140 (MQ=255)

                                                                               gTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGa                                                       >  1:1883649/1‑140 (MQ=255)

                                                                                  gAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAAtt                                                    <  1:2049947/140‑1 (MQ=255)

                                                                                  gAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAAtt                                                    >  1:209322/1‑140 (MQ=255)

                                                                                   aGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATTGGACAAGTGAAGGCGTTGACGCCCTTCAGGAATTc                                                   >  2:1079682/1‑140 (MQ=255)

                                                                                     tAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCaa                                                 >  1:614182/1‑140 (MQ=255)

                                                                                        cAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAAt                                              >  1:1659313/1‑140 (MQ=255)

                                                                                        cAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAAt                                              <  1:1259471/140‑1 (MQ=255)

                                                                                          gACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTg                                            >  2:2240594/1‑140 (MQ=255)

                                                                                           aCCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTgg                                           <  1:2240594/140‑1 (MQ=255)

                                                                                            ccAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGa                                          <  1:2115147/140‑1 (MQ=255)

                                                                                            ccAAGATGTTTCGCTGTGGCTTAGCCAAACACACGCATTGTAATCGGCTTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACTCCTTTCAGGAATTCAAAATTGGa                                          <  2:847527/140‑1 (MQ=255)

                                                                                             cAAGATGTTTCGCTGTGGCTTCGCCTAACTTACGCATCTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGAt                                         <  1:1079682/140‑1 (MQ=255)

                                                                                             cAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGAt                                         >  2:2392556/1‑140 (MQ=255)

                                                                                             cAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGAt                                         <  1:2443893/140‑1 (MQ=255)

                                                                                                  tGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTAtt                                    <  2:115373/140‑1 (MQ=255)

                                                                                                   gTTTCGCTGTGGCTTGGCCAAACATCCGCCTTTTAATGGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATtc                                   <  1:805150/140‑1 (MQ=255)

                                                                                                     ttCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCGTTCGGGAATTCAAAATTGGATTTATtcat                                 >  1:849011/1‑140 (MQ=255)

                                                                                                     ttCGCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGAt                                         <  2:295355/132‑1 (MQ=255)

                                                                                                        gCTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTcatcat                              <  2:1766409/140‑1 (MQ=255)

                                                                                                         cTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCAtt                             <  2:199356/140‑1 (MQ=255)

                                                                                                         cTGTGGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTAATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCAtt                             <  2:1418216/140‑1 (MQ=255)

                                                                                                         cTGTCGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCAtt                             <  2:170920/140‑1 (MQ=255)

                                                                                                          tgtgGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTg                            <  2:2031203/140‑1 (MQ=255)

                                                                                                          tgtgGCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTg                            >  1:303872/1‑140 (MQ=255)

                                                                                                              gCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTAt                                     >  1:138084/1‑127 (MQ=255)

                                                                                                              gCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTAt                                     <  2:138084/127‑1 (MQ=255)

                                                                                                              gCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGttat                        <  2:1987012/140‑1 (MQ=255)

                                                                                                              gCTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGttat                        <  2:1916573/140‑1 (MQ=255)

                                                                                                               cTTCGCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATtcatca                               <  2:1303522/132‑1 (MQ=255)

                                                                                                                   gCCAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAGATTCGAGTTATTCGTCATTGTTATTAATc                   >  1:1170048/1‑140 (MQ=255)

                                                                                                                     cAAACATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCa                 <  2:209322/140‑1 (MQ=255)

                                                                                                                        aCATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTg              <  2:1315224/140‑1 (MQ=255)

                                                                                                                        aCATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTg              <  1:371731/140‑1 (MQ=255)

                                                                                                                        aCATACGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTg              <  1:1228702/140‑1 (MQ=255)

                                                                                                                        aCATACGCATTTTAATCCGCGTTGTGTTGGGATCAACCGTGTAGTTATGTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTg              <  2:868727/140‑1 (MQ=255)

                                                                                                                             cGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCtgtg         >  1:1483003/1‑140 (MQ=255)

                                                                                                                             cGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCtgtg         >  1:1432646/1‑140 (MQ=255)

                                                                                                                             cGCATTTTAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCATTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCtgtg         <  2:2306635/140‑1 (MQ=255)

                                                                                                                             cGCATTTTAATCGGCGTCGTGTGGGGATCATCCGGCTAGGTATTTTCCCCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCtgtg         <  2:793072/140‑1 (MQ=255)

                                                                                                                                  tttAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGc    >  1:524617/1‑140 (MQ=255)

                                                                                                                                  tttAATCGGCGTCGTGTTGGGACCATCCGGGCAGTCATTCTCCGCCGCCCAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGc    <  2:651284/140‑1 (MQ=255)

                                                                                                                                  tttAATCGGCGTCGTATTGGGAACATCCGAGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGc    <  1:843855/140‑1 (MQ=255)

                                                                                                                                   ttAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGcc   >  2:563975/1‑140 (MQ=255)

                                                                                                                                   ttAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGcc   <  2:614182/140‑1 (MQ=255)

                                                                                                                                    tAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGCCt  >  1:951897/1‑140 (MQ=255)

                                                                                                                                    tAATCGGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTGATTCATCTTTGATAATAATCCATTGCTGTGCGGGCCt  >  1:651284/1‑140 (MQ=255)

CAGAGAGGATGTTGTCATCAACAAAGGCGATTTTGCCGAGTTCACGCAGGAGATCGTGTTGATTCTCACAAGGGGGGATGTTGAGTAACAGACCAAGATGTTTCGCTGTGGCTTCGCCAAACATACGCATTTTAATCAGCGTCGTGTTGGGATCATCCGGGTAGTTATTTTCCGCCGCACAGGCGATGGCATAAGTGAAGTCGTTGACGCCCTTCAGGAATTCAAAATTGGATTTATTCATCATTGTTATTAATCCATTGCTGTGCGGGCCT  >  NC_000913/4586518‑4586789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`