BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,590,443	T→C	A279A (GCA→GCG)	btsT ←	pyruvate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,590,443	0	T	C	98.6%	493.6 / NA	144	A279A (GCA→GCG)	btsT	pyruvate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (64/77); minor base A (2/0); total (66/78)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAG  >  NC_000913/4590306‑4590579

ccTTTCCACAGCGGGTCAGTACCGTTAATGTACTGGGACATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGgcgc                                                                                                                                        <  1:834750/140‑1 (MQ=255)

 cTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCgcgcggcgcg                                                                                                                                       <  2:2440919/140‑1 (MQ=255)

    tCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAgg                                                                                                                                    <  2:196489/140‑1 (MQ=255)

    tCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGAGTTTCAGGCAGGATGCCAGATAGTCGCGCGGCGCGAgg                                                                                                                                    >  1:915461/1‑140 (MQ=255)

           cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGGTAGTCGCGCGGCGCGAGGATCAGcc                                                                                                                             >  1:989553/1‑140 (MQ=255)

           cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAgg                                                                                                                                    >  1:588808/1‑133 (MQ=255)

           cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAgg                                                                                                                                    <  2:588808/133‑1 (MQ=255)

           cGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACC‑‑‑‑‑ACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACt                                                                                                                        >  2:1718849/1‑140 (MQ=255)

             ggCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCcac                                                                                                                           >  2:2149739/1‑140 (MQ=255)

             ggCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCACGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCcac                                                                                                                           <  2:1560909/140‑1 (MQ=255)

                cAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGa                                                                                                                                      <  1:336787/126‑1 (MQ=255)

                cAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGa                                                                                                                                      >  2:336787/1‑126 (MQ=255)

                cAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACC‑‑‑‑‑ACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGgcag                                                                                                                   <  1:1718849/140‑1 (MQ=255)

                     ccGTCAATGTACTGGGTGATGGCAGGCATTTCCCGCTCCGGGTTCAGCTCCACGATACCCAGCGCCAGGACGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGgcag                                                                                                                   <  2:1154006/140‑1 (MQ=255)

                     ccGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTTCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGgcag                                                                                                                   <  1:920799/140‑1 (MQ=255)

                     ccGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGgcag                                                                                                                   <  1:2294702/140‑1 (MQ=255)

                      cGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGgcagc                                                                                                                  <  1:2212169/140‑1 (MQ=255)

                        tCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCTGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTGGTGCGGCGCGTGGATGAGCCACACAGGcagcag                                                                                                                >  2:1198455/1‑140 (MQ=255)

                        tCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGcagcag                                                                                                                >  2:606354/1‑140 (MQ=255)

                        tCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGcagcag                                                                                                                >  1:476304/1‑140 (MQ=255)

                        tCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAAATAGTCGAGCGGCGCGGGGATTAGCCACACTGGcagcag                                                                                                                >  2:1057384/1‑140 (MQ=255)

                            tGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCg                                                                                                            >  1:1641295/1‑140 (MQ=255)

                             gTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCCCGCGGCGCGAGTATCAGCCACGCTGGCAGCAGTGCgg                                                                                                           >  1:1048248/1‑140 (MQ=255)

                              tACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCAAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGa                                                                                                          <  1:1955505/140‑1 (MQ=255)

                                 tGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaac                                                                                                       >  2:159043/1‑140 (MQ=255)

                                 tGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaac                                                                                                       >  2:2235990/1‑140 (MQ=255)

                                  gggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaaca                                                                                                      >  2:1309427/1‑140 (MQ=255)

                                  gggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaaca                                                                                                      <  1:1430949/140‑1 (MQ=255)

                                  gggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaaca                                                                                                      <  1:2235990/140‑1 (MQ=255)

                                  gggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaaca                                                                                                      >  2:1814355/1‑140 (MQ=255)

                                   ggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaacaa                                                                                                     >  2:2393456/1‑140 (MQ=255)

                                   ggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaacaa                                                                                                     >  2:2387757/1‑140 (MQ=255)

                                   ggTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGAAAGTCGCGCCGCGAGAGGATCAGCCACACTGACAGCAGTGCGGaaacaa                                                                                                     >  2:937159/1‑140 (MQ=255)

                                    gTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaacaaa                                                                                                    <  1:853530/140‑1 (MQ=255)

                                    gTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGaaacaaa                                                                                                    >  1:608017/1‑140 (MQ=255)

                                     tCATGGCAGGCA‑TTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  <  1:571453/140‑1 (MQ=255)

                                      cATGGCAGGCATTTTTAGTTCCTGGCTCACCACCACGCTACCCAGCGCCAGGCCGTCGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACTCTGGCAGCAGTGCGGAAACAAACg                                                                                                  <  1:655517/140‑1 (MQ=255)

                                      cATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  >  1:1451071/1‑140 (MQ=255)

                                      cATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  >  2:422954/1‑140 (MQ=255)

                                      cATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  >  1:298419/1‑140 (MQ=255)

                                      cATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  <  2:1906348/140‑1 (MQ=255)

                                      cATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  <  1:229218/140‑1 (MQ=255)

                                      cATGGCAGGCATTTTCACTTCCTGGTTCAGCCCCATGATTCCCAGCGCTAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACg                                                                                                  <  2:757729/140‑1 (MQ=255)

                                        tGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACGCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCa                                                                                                >  2:1430934/1‑140 (MQ=255)

                                        tGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCa                                                                                                <  1:1784250/140‑1 (MQ=255)

                                        tGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCa                                                                                                <  1:2387757/140‑1 (MQ=255)

                                        tGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCa                                                                                                >  1:2454379/1‑140 (MQ=255)

                                        tGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGCTGCCAGATAGCCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGcc                                                                                                >  2:549723/1‑139 (MQ=255)

                                              gcaTTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCa                                                                                          >  2:325334/1‑140 (MQ=255)

                                               caTTTTCAGTTCCGGGTTCAGCACCACGCTACCCAGCGCAAGGCCGACGATACCGCCGATTTTAAGGACGATTGCCAGCTAGCCGCGCGCCCCGAGGGTCAGACTCCCGGGCAGCAGAGCGGGAAAAAACGCATAGccga                                                                                         >  2:655517/1‑138 (MQ=255)

                                                aTTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAAt                                                                                        <  2:974373/140‑1 (MQ=255)

                                                   ttCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAg                                                                                     >  2:1546679/1‑140 (MQ=255)

                                                     cAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcg                                                                                   <  2:2469934/140‑1 (MQ=255)

                                                     cAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcg                                                                                   <  2:1479634/140‑1 (MQ=255)

                                                      aGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcgc                                                                                  <  1:1546679/140‑1 (MQ=255)

                                                      aGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcgc                                                                                  <  1:1752298/140‑1 (MQ=255)

                                                      aGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAgcgc                                                                                  <  2:1472976/140‑1 (MQ=255)

                                                       gTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGcgcg                                                                                 <  1:422954/140‑1 (MQ=255)

                                                        ttCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGa                                                                                <  2:2460735/140‑1 (MQ=255)

                                                         tCCGGGTTCAGCTCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCCCACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGaa                                                                               <  1:1362530/140‑1 (MQ=255)

                                                         tCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGaa                                                                               <  1:510388/140‑1 (MQ=255)

                                                             ggTTCAGCTCCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTa                                                                           <  2:344289/140‑1 (MQ=255)

                                                             ggTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCAGACTGGCAGGAGTGCGGAAACAAACGGATAGCCAATCAGCGCGAAGGTa                                                                           >  2:651954/1‑140 (MQ=255)

                                                             ggTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTa                                                                           >  2:5415/1‑140 (MQ=255)

                                                             ggTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTa                                                                           >  1:491993/1‑140 (MQ=255)

                                                                    caccacGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAAtggtgg                                                                    <  1:606354/140‑1 (MQ=255)

                                                                    caccacGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAAtggtgg                                                                    >  2:1725337/1‑140 (MQ=255)

                                                                     accacGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATggtggt                                                                   <  2:179328/140‑1 (MQ=255)

                                                                       cacGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGCCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGgtgt                                                                 >  2:1414927/1‑140 (MQ=255)

                                                                         cGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCt                                                               >  2:2422764/1‑140 (MQ=255)

                                                                            tACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCCGAAACAAACGCATAGCGAATCAGCGCGAAGTTAATGGTGGTGTCTTTa                                                            >  2:913851/1‑140 (MQ=255)

                                                                                   cgcCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCa                                                     >  1:1282377/1‑140 (MQ=255)

                                                                                      cAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTg                                                  >  2:2287608/1‑140 (MQ=255)

                                                                                        ggCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGcc                                                >  2:86508/1‑140 (MQ=255)

                                                                                         gCCGATGATAACGCCGATTTTCAGGAAGGTTGCCCGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCg                                               <  2:2281871/140‑1 (MQ=255)

                                                                                         gCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCg                                               <  2:446355/140‑1 (MQ=255)

                                                                                         gCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCg                                               <  2:1282377/140‑1 (MQ=255)

                                                                                         gCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCg                                               <  1:1832203/140‑1 (MQ=255)

                                                                                         gCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCg                                               <  1:304813/140‑1 (MQ=255)

                                                                                         gCCGACGATAACGCCGACTTTCCGGAAGGTTACTATATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTgtcg                                               <  2:1109705/140‑4 (MQ=255)

                                                                                             acgaTAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGAcc                                           <  1:1559192/140‑1 (MQ=255)

                                                                                             acgaTAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGAcc                                           <  1:549723/140‑1 (MQ=255)

                                                                                             acgaTAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGAcc                                           <  2:491993/140‑1 (MQ=255)

                                                                                              cgaTAACGTCGATTTTCAGGAAGGTTGCCAGCTCGACGCACGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGAccc                                          <  2:1130781/140‑1 (MQ=255)

                                                                                               gaTAACGCCGATTTTCATGAAGATTGCCAGATAGTTGCGTGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGAcccc                                         <  2:803680/140‑1 (MQ=255)

                                                                                                    cGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTAc                                    <  2:1893646/140‑1 (MQ=255)

                                                                                                    cGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTAc                                    >  2:351592/1‑140 (MQ=255)

                                                                                                       cGATTTTCAGGACGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGa                                 <  1:1907745/140‑1 (MQ=255)

                                                                                                       cGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGa                                 <  1:1309427/140‑1 (MQ=255)

                                                                                                        gATTTTCAGGAAGGTAGCCAGATAGTCGCGCGGCGCGTGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGAt                                <  1:1057384/140‑1 (MQ=255)

                                                                                                          ttttCAGGAAGGTTGCCAGATACTCCCGCCGCGCGAGCATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCg                              <  1:651954/140‑1 (MQ=255)

                                                                                                                        gCCAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCCGTGCCGGGCCCGAGTACGGATAGTGAGCAATCACGcc                >  1:829389/1‑140 (MQ=255)

                                                                                                                         ccAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCa               <  1:325334/140‑1 (MQ=255)

                                                                                                                         ccAGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCa               <  1:86508/140‑1 (MQ=255)

                                                                                                                           aGATAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCAcc             <  1:1400117/140‑1 (MQ=255)

                                                                                                                              tAGTCGCGTGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGaa          <  1:422111/140‑1 (MQ=255)

                                                                                                                              tAGTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGaa          <  1:1814355/140‑1 (MQ=255)

                                                                                                                                gTCGCGCGGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGt        >  2:2256012/1‑140 (MQ=255)

                                                                                                                                    cgcgGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGtagat    >  2:485428/1‑140 (MQ=255)

                                                                                                                                      cgGCGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCCATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGtagatag  >  2:153746/1‑140 (MQ=255)

CCTTTCCACAGCGGGCCAGTACCGTCAATGTACTGGGTCATGGCAGGCATTTTCAGTTCCGGGTTCAGCACCACGATACCCAGCGCCAGGCCGACGATAACGCCGATTTTCAGGAAGGTTGCCAGATAGTCGCGCGGTGCGAGGATCAGCCACACTGGCAGCAGTGCGGAAACAAACGCATAGCCAATCAGCGCGAAGGTAATGGTGGTGTCTTTAAAGGTCAGTGCCGGACCCCAGTACGGATCGTGAGCAATCACGCCACCGAAGTAGATAG  >  NC_000913/4590306‑4590579

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`