Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,593,296	A→G	N547S (AAC→AGC)	tsr →	methyl‑accepting chemotaxis protein Tsr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,593,296	0	A	G	100.0%	328.6 / NA	92	N547S (AAC→AGC)	tsr	methyl‑accepting chemotaxis protein Tsr
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (51/41);  total (51/41)

AGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGT  >  NC_000913/4593164‑4593429                                                                                                                                     |                                                                                                                                      agCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGaa                                                                                                                                <  2:2029476/140‑1 (MQ=255) agCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGaa                                                                                                                                <  1:2268309/140‑1 (MQ=255)     ggCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACAt                                                                                                                            >  1:2276467/1‑140 (MQ=255)        cAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACAtttt                                                                                                                         >  1:249153/1‑140 (MQ=255)           tCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAAt                                                                                                                      >  2:2243102/1‑140 (MQ=255)           tCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAAt                                                                                                                      <  1:2128924/140‑1 (MQ=255)           tCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAAt                                                                                                                      >  2:2365772/1‑140 (MQ=255)             gCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAAt                                                                                                                      <  2:2182029/138‑1 (MQ=255)             gCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAAt                                                                                                                      >  1:2182029/1‑138 (MQ=255)               cTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGcc                                                                                                                  <  1:1683278/140‑1 (MQ=255)                tGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCa                                                                                                                 >  2:1306645/1‑140 (MQ=255)                  aCCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGTCTGTGGTAAAAACCGTGACGCCAGCTGCGCGGCGTAAAATGGCCGTGGCAGATAGCGAGGAGGGCTGGGAAACATTTTAATCGCCATg                                                                                                               >  1:1298613/1‑140 (MQ=255)                  aCCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAAAGGCCGTGGGAGACAGCAAGGAGAGCCGGGAAACATTTTAATCGCCATg                                                                                                               >  2:1105668/1‑140 (MQ=255)                    cGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGaa                                                                                                             <  1:254455/140‑1 (MQ=255)                            tggcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGGGAGCTGGCAAAGATTTTAATCGCCATGAAAATGCCCg                                                                                                     >  1:832451/1‑140 (MQ=255)                            tggcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGCGAGCTGGGAAAGATATTAATCGCCATGAAAATGCCCg                                                                                                     >  1:740748/1‑140 (MQ=255)                            tggcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCg                                                                                                     >  2:1778217/1‑140 (MQ=255)                             ggcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGa                                                                                                    <  1:1370116/140‑1 (MQ=255)                             ggcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGa                                                                                                    <  1:2365772/140‑1 (MQ=255)                              gcagtgTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGAt                                                                                                   <  1:112950/140‑1 (MQ=255)                                   gtTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCa                                                                                              <  1:1443116/140‑1 (MQ=255)                                    ttCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGGaa                                                                                             >  2:2473191/1‑140 (MQ=255)                                     tCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCaaa                                                                                            >  2:2120715/1‑140 (MQ=255)                                      ccGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGTAAAAATTCTAATCGCCCTGAAAATGCCCGATAAGCaaaa                                                                                           >  2:848022/1‑140 (MQ=255)                                      ccGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAACTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCaaaa                                                                                           >  2:1745263/1‑140 (MQ=255)                                            tCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt                                                                                     <  1:1778217/140‑1 (MQ=255)                                            tCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt                                                                                     <  1:2464513/140‑1 (MQ=255)                                            tCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt                                                                                     <  1:2293753/140‑1 (MQ=255)                                            tCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt                                                                                     <  1:321080/140‑1 (MQ=255)                                            tCAGCAACAGCAGCGTGAAACAACGGCTGTGCTAACAACCGTGACGCCTGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTAt                                                                                     <  2:832451/140‑1 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGTGAAACATTTTAAGCGCGATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  2:747160/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  1:2125448/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  1:1734584/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  1:345090/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  2:1507588/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCg                                                                                   >  1:511893/1‑140 (MQ=255)                                              aGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTCTAAACGCAATGAAAATCCCCGTTAAGCAAAATGTTATCg                                                                                   >  2:1024407/1‑140 (MQ=255)                                                cAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCggg                                                                                 <  1:1502137/140‑1 (MQ=255)                                                     gcagcGTGAAACATCGGCTGTGGTAAAAACCGTGACGTCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa                                                                            <  1:1105668/140‑1 (MQ=255)                                                     gcagcGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa                                                                            <  2:249153/140‑1 (MQ=255)                                                      cagcGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAACTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAg                                                                           <  1:1745263/140‑1 (MQ=255)                                                        gcGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa                                                                            <  2:456434/137‑1 (MQ=255)                                                        gcGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATaa                                                                            >  1:456434/1‑137 (MQ=255)                                                        gcGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGga                                                                         >  1:2167505/1‑140 (MQ=255)                                                          gTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCggg                                                                                 <  2:2280942/130‑1 (MQ=255)                                                          gTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCggg                                                                                 >  1:2280942/1‑130 (MQ=255)                                                           tGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGAt                                                                      <  2:2125448/140‑1 (MQ=255)                                                           tGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGAt                                                                      <  1:1893876/140‑1 (MQ=255)                                                           tGAAACATCGGCTGTGGCAAAAACCGTGACGACAGCTGCCCCGCGTAATATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGAt                                                                      <  2:740748/140‑1 (MQ=255)                                                             aaaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCAt                                                                              <  2:1897684/130‑1 (MQ=255)                                                             aaaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCAt                                                                              >  1:1897684/1‑130 (MQ=255)                                                              aaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGc                                                                                <  2:2139409/127‑1 (MQ=255)                                                              aaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGc                                                                                >  1:2139409/1‑127 (MQ=255)                                                              aaCATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGACCGATAAACAATATATTATCGAGCATAAGGAGATTaa                                                                   >  1:1055881/1‑140 (MQ=255)                                                                   cGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGGAGAATGTTATGGGGCTAAAGGAGATTAATCttt                                                              >  2:1129921/1‑140 (MQ=255)                                                                   cGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCttt                                                              >  1:436379/1‑140 (MQ=255)                                                                   cGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCttt                                                              >  2:1037457/1‑140 (MQ=255)                                                                     gCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTAc                                                            >  2:288272/1‑140 (MQ=255)                                                                     gCTGTGGTAAAAACCGTGACGCAAGTTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTAc                                                            <  1:1086717/140‑1 (MQ=255)                                                                     gCTGTGGTAAAAACCGTGACCCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTAc                                                            >  1:28958/1‑140 (MQ=255)                                                                      cTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACg                                                           <  1:1507588/140‑1 (MQ=255)                                                                      cTGTGGTAAAAACCGAGACGCCCGCTGCGACGCGTAAGATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACg                                                           <  1:1028725/140‑1 (MQ=255)                                                                       tgtgGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGGAAAATGTGATCGGGGATAAGGGGATTAATCTTTACGt                                                          >  1:695175/1‑140 (MQ=255)                                                                        gtgGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACg                                                           >  1:322694/1‑138 (MQ=255)                                                                        gtgGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACg                                                           <  2:322694/138‑1 (MQ=255)                                                                         tgGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTgg                                                        >  2:1803342/1‑140 (MQ=255)                                                                          ggTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTggg                                                       >  2:499025/1‑140 (MQ=255)                                                                                    gTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGTGCATAAGGAGACTAGTCTTTACGTGGGTCGTTGATCg                                             >  1:1158168/1‑140 (MQ=255)                                                                                     tGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAGATGCCCGATGAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCgg                                            >  2:1993033/1‑140 (MQ=255)                                                                                     tGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCgg                                            >  2:2304889/1‑140 (MQ=255)                                                                                     tGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCgg                                            >  2:1310002/1‑140 (MQ=255)                                                                                     tGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCgg                                            >  1:1882165/1‑140 (MQ=255)                                                                                     tGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGACATAAGGAGATTAATCTTTACGTGGGTCGTTGATCgg                                            >  2:1815433/1‑140 (MQ=255)                                                                                           cAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACg                                      <  1:1803342/140‑1 (MQ=255)                                                                                           cAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACg                                      <  1:2120715/140‑1 (MQ=255)                                                                                            aGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGAAGATTAAACTTTACGTGGGTCGTTGATCGGCTGACGa                                     >  1:1005329/1‑140 (MQ=255)                                                                                             gCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGaa                                    <  2:511893/140‑1 (MQ=255)                                                                                              cTGCGCCGCGTAAAATGGCCGTTGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAAc                                   <  1:1015379/140‑1 (MQ=255)                                                                                                   ccgcGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGa                              <  2:2167505/140‑1 (MQ=255)                                                                                                   ccgcGTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGa                              <  2:345090/140‑1 (MQ=255)                                                                                                       gTAAAATGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGTTCGCTGATCGGCTTACGAACCAGGAAGAt                          >  1:762963/1‑140 (MQ=255)                                                                                                         aaaaTGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGt                        <  1:2304889/140‑1 (MQ=255)                                                                                                          aaaTGGCCGTGGCAGATGGCGACGAGAGCTGAGAAACATATTAATCGCGATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTa                       <  2:762963/140‑1 (MQ=255)                                                                                                          aaaTGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTa                       <  1:1306645/140‑1 (MQ=255)                                                                                                           aaTGGCCGTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTAc                      >  1:224243/1‑140 (MQ=255)                                                                                                                  gTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTg               >  2:247140/1‑140 (MQ=255)                                                                                                                  gTGGCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTg               >  1:2474623/1‑140 (MQ=255)                                                                                                                   tGGCAGATAGCGAGGAGAGCTGAGATACACTTTAATCTCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGc              <  1:1129921/140‑1 (MQ=255)                                                                                                                   tGGCAGATAGCGAGGAGAGCGGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGc              <  1:2260302/140‑1 (MQ=255)                                                                                                                     gCAGATAGCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGccc            >  1:2102369/1‑140 (MQ=255)                                                                                                                            gCGAGGAGAGCTGGGGAACATTATATTCGTCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGc     <  1:950697/140‑1 (MQ=255)                                                                                                                            gCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGc     <  1:1037457/140‑1 (MQ=255)                                                                                                                            gCGAGGAGAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCGGACGAACGAGGTAGGTGTACGTCAGTGCCCCCGCTGc     >  1:645411/1‑140 (MQ=255)                                                                                                                               aggagAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGt  >  2:1671015/1‑140 (MQ=255)                                                                                                                               aggagAGCTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTAGATCGGCTGACGCACCAGGAAGATGAACGCAAGTGCCCCCGCTGCGGt  >  2:925257/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      AGCAGGCCAGTCGCCTGACCGAAGCAGTGGCAGTGTTCCGGATTCAGCAACAGCAGCGTGAAACATCGGCTGTGGTAAAAACCGTGACGCCAGCTGCGCCGCGTAAAATGGCCGTGGCAGATAGCGAGGAGAACTGGGAAACATTTTAATCGCCATGAAAATGCCCGATAAGCAAAATGTTATCGGGCATAAGGAGATTAATCTTTACGTGGGTCGTTGATCGGCTGACGAACCAGGAAGATGTACGCCAGTGCCCCCGCTGCGGT  >  NC_000913/4593164‑4593429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑