BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,604,288	T→C	S43S (AGT→AGC)	bglJ →	DNA‑binding transcriptional regulator BglJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,288	0	T	C	100.0%	586.1 / NA	166	S43S (AGT→AGC)	bglJ	DNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (95/71); total (95/71)

Only 100 of 172 total aligned reads displayed.

TGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGG  >  NC_000913/4604151‑4604423

tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCa                                                                                                                                        >  1:2387468/1‑140 (MQ=255)

tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCa                                                                                                                                        >  1:1981862/1‑140 (MQ=255)

tGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTCTGCAGGTAACCCTTATGAGCTCTATACCTATACCAGCCa                                                                                                                                        >  2:1121352/1‑140 (MQ=255)

   cGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGa                                                                                                                                     <  2:1246875/140‑1 (MQ=255)

   cGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGa                                                                                                                                     <  2:1576052/140‑1 (MQ=255)

   cGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGa                                                                                                                                     <  2:2487721/140‑1 (MQ=255)

    gCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAg                                                                                                                                    <  2:1851375/140‑1 (MQ=255)

    gCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAg                                                                                                                                    <  2:2453427/140‑1 (MQ=255)

     cAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTGTAATCTGCATACCGATACCAGCCAGGAGt                                                                                                                                   >  1:670621/1‑140 (MQ=255)

     cAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGCAACCCTTCTAAGCTGCATACCGATACCAGCCAGGAGt                                                                                                                                   >  1:1136642/1‑140 (MQ=255)

                  aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCa                                                                                                                            <  2:1766687/134‑1 (MQ=255)

                  aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGc                                                                                                                      >  2:2022943/1‑140 (MQ=255)

                  aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGc                                                                                                                      >  1:1257008/1‑140 (MQ=255)

                  aGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGc                                                                                                                      >  2:1537965/1‑140 (MQ=255)

                    ccGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCa                                                                                                                    >  2:3835/1‑140 (MQ=255)

                    ccGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCa                                                                                                                    >  1:479016/1‑140 (MQ=255)

                    ccGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCa                                                                                                                    <  1:355389/140‑1 (MQ=255)

                      gAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCGTACCTATACCAGCCGGGAGTCGTTTCAGGATGCCATg                                                                                                                  >  1:1194108/1‑140 (MQ=255)

                         ttAAGAAGCGAAATGTGTCACTTATAGAAATGTGCGTCACGAGTAGTAGCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTcg                                                                                                               <  2:1205195/140‑1 (MQ=255)

                              aagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTGTACGAGCAAGGAGTCAATTCAAGATGCCATGTCGCGGAt                                                                                                          >  2:648116/1‑140 (MQ=255)

                              aagaGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGAt                                                                                                          >  2:1454242/1‑140 (MQ=255)

                                        tCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTAAACCAGCCATGAGTCATTTCAGGATGACATGTCGCGGATCTCGTTTgcg                                                                                                >  2:858358/1‑140 (MQ=255)

                                         cGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcgg                                                                                               >  2:520726/1‑140 (MQ=255)

                                           cACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggcg                                                                                             <  2:1627802/140‑1 (MQ=255)

                                           cACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggcg                                                                                             >  2:1945671/1‑140 (MQ=255)

                                           cACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTgcggcg                                                                                             <  1:520726/140‑1 (MQ=255)

                                            aCTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGcggcgg                                                                                            <  2:2277360/140‑1 (MQ=255)

                                                  aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAt‑tt                                                                                     >  2:2267796/1‑140 (MQ=255)

                                                  aGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTAAGGGTGCCTTGTCGCGTATCTGGTTTGCGGCGGTCAt‑tt                                                                                     >  2:1189316/1‑140 (MQ=255)

                                                        aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttttt                                                                               >  1:318649/1‑140 (MQ=255)

                                                          gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctttt                                                                             <  2:2014404/140‑1 (MQ=255)

                                                          gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttctttt                                                                             >  1:484936/1‑140 (MQ=255)

                                                           cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTGAGGATGCCGTGTAGCGCATCTCGTTTGCGGAGGTCAt‑ttttttttttt                                                                            >  2:687242/1‑140 (MQ=255)

                                                           cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑tttttcttttt                                                                            <  1:529579/140‑1 (MQ=255)

                                                             tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTCTACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                          <  1:1319377/140‑1 (MQ=255)

                                                             tCATGAGTAGTATCGCTATTGATAGATTACTCAGAAACTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑Tttttctttttct                                                                          <  1:687242/140‑1 (MQ=255)

                                                              cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCt‑tttttttttttttt                                                                         >  2:2294956/1‑137 (MQ=255)

                                                              cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAA‑tttttttttttttt                                                                         >  2:1319377/1‑137 (MQ=255)

                                                               aTGAGTAGTATCGGTTTTGAGATTTTATTCAGAAAGTCTGCGGGTAACCCTTATAAGCTCCATACCTTTACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGc                                                                        <  1:1098088/140‑1 (MQ=255)

                                                                    tagtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATga                                                                   <  2:2254480/140‑1 (MQ=255)

                                                                     agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCT‑TTTTTTTTTTTTTTCCCTgag                                                                  >  2:237024/1‑140 (MQ=255)

                                                                     agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATgag                                                                  >  1:1662278/1‑140 (MQ=255)

                                                                        aTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGAAg                                                               >  2:1288295/1‑140 (MQ=255)

                                                                         tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTgaggagg                                                              >  1:1706324/1‑139 (MQ=255)

                                                                         tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAgg                                                              >  1:154208/1‑139 (MQ=255)

                                                                           ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGa                                                            <  1:1945671/140‑1 (MQ=255)

                                                                            gTATTGAGAGTTTATTCAGAAGTTTTGCGCGTAACCCGTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAg                                                           <  1:931414/140‑1 (MQ=255)

                                                                            gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGAAGTGAg                                                           >  2:480151/1‑140 (MQ=255)

                                                                            gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAg                                                           >  2:1679366/1‑140 (MQ=255)

                                                                            gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAg                                                           >  2:1267138/1‑140 (MQ=255)

                                                                            gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCCGCGGTCTG‑CTTGTAATTTTATACCAAGAGAAGTGAg                                                           >  1:2177521/1‑140 (MQ=255)

                                                                             tATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAgc                                                          <  2:95563/140‑1 (MQ=255)

                                                                              aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAAGGAgcg                                                         >  2:412066/1‑140 (MQ=255)

                                                                                tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                       <  1:1936428/140‑1 (MQ=255)

                                                                                tGAGAGTTTATTCAGAAAGTTTCCGTGTAGCCCTTATCAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCa                                                       <  1:790425/140‑1 (MQ=255)

                                                                                 gagaGTTTATTCAGACAGTTTGCGGATAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                      <  1:1041028/140‑1 (MQ=255)

                                                                                 gagaGTTTATTCAGAAAGTTTGTGGGTAAACCTTATATGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCag                                                      <  1:1121352/140‑1 (MQ=255)

                                                                                 gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATATTTTTTTTTTTCTG‑CATGTGAACTGAGCGGag                                                      >  2:1506020/1‑140 (MQ=255)

                                                                                 gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTAGTTTGCGGCGGTTAT‑TCTTTCTTTTGTTTCGCTGAGAAGAGAGCGCag                                                      >  2:1036025/1‑140 (MQ=255)

                                                                                  agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                     >  1:1519367/1‑140 (MQ=255)

                                                                                   gagTTTATTCAGAAAGTTTGCGGGTGACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                    <  1:851654/140‑1 (MQ=255)

                                                                                   gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                    <  1:2022943/140‑1 (MQ=255)

                                                                                   gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                    <  1:1454242/140‑1 (MQ=255)

                                                                                   gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                    <  1:2031790/140‑1 (MQ=255)

                                                                                   gagTTTATTCAGAAACTTTGCGCGTCACCATTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCagag                                                    <  1:643685/140‑1 (MQ=255)

                                                                                     gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTCGCGGCGGTACT‑TTTTTCTTTTTTTTGCCTGGGAAGAGAGCGCagggag                                                  >  2:1041028/1‑140 (MQ=255)

                                                                                      tttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCACGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAgg                                                 >  1:1390069/1‑140 (MQ=255)

                                                                                       ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTTTTCCCTGAGAAGTGAGGGCAGAGAggg                                                >  2:2361385/1‑140 (MQ=255)

                                                                                       ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                     >  1:185061/1‑135 (MQ=255)

                                                                                       ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                <  1:17721/140‑1 (MQ=255)

                                                                                       ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAggg                                                >  1:1584856/1‑140 (MQ=255)

                                                                                        tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCCTGAGAAGGGAGCGCGGAGAgggg                                               >  1:1415360/1‑139 (MQ=255)

                                                                                         aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCC‑TTTTTTTTTGTTTTTCATGTGAAAGGGGCGCAGAGAGGGaa                                              >  2:769079/1‑139 (MQ=255)

                                                                                         aTTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggggg                                              >  2:1844102/1‑138 (MQ=255)

                                                                                           tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTa                                            <  2:1390069/140‑1 (MQ=255)

                                                                                               aaaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATACCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCCTGAGAAGTGAGGGCAGAGGGGGGTTATCtt                                        >  2:1954381/1‑140 (MQ=255)

                                                                                                aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                       >  1:117629/1‑140 (MQ=255)

                                                                                                aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                       <  1:1288295/140‑1 (MQ=255)

                                                                                                aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                       <  2:369123/140‑1 (MQ=255)

                                                                                                 aGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                      <  2:303362/140‑1 (MQ=255)

                                                                                                    ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGGGAGGGCAGGGGGGGATTATCCTgtttt                                   >  2:1894899/1‑136 (MQ=255)

                                                                                                    ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTGTTTTTTTTGTCCTTGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctt                                   >  2:640477/1‑139 (MQ=255)

                                                                                                      gCGGGTAACCCTTATCAGCTCCCTATCTAAACCAGCCCGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgac                                 <  1:648116/140‑1 (MQ=255)

                                                                                                           tAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATTTTTCCTGACTTaaa                            >  1:2437053/1‑139 (MQ=255)

                                                                                                           tAACCCTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAAc                            <  2:203190/140‑1 (MQ=255)

                                                                                                             aCCCTTATAAGCTCCATACCTATACCAGCTAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTTCCATGAGAAGTGAGGGGAGAGAGGGGTTATCTTGTCTTTCTGTACTg                          >  2:2383619/1‑140 (MQ=255)

                                                                                                               ccTTATAAGCTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                        <  1:1581976/140‑1 (MQ=255)

                                                                                                                   ataAGCTCCATACCTATCCCAGCCAGGAGTCCTTTCAGGCTGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                    <  2:670621/140‑1 (MQ=255)

                                                                                                                        cTCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGtt               <  2:2398151/140‑1 (MQ=255)

                                                                                                                         tCCATACCTATACCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGttt              >  2:113539/1‑140 (MQ=255)

                                                                                                                                 tataCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAc      <  1:2361385/140‑1 (MQ=255)

                                                                                                                                  ataCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc     <  1:2267397/140‑1 (MQ=255)

                                                                                                                                  ataCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAcc     <  1:412066/140‑1 (MQ=255)

                                                                                                                                   taCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGAC‑TTTTTTTTTTTTTTCCCTGGGGACGGAGGGCAGAGGGGGGTTTTCTTTTCTGACTTGACTTGCGGTTAAGTTTTcgccggtcc    >  2:52154/1‑134 (MQ=255)

                                                                                                                                   taCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTTTGCCATGAGAAGTGAGCGGCGAGAGGGGTTAGTTTGTCCGGATGGACTTGCGCGTAAGGTTCCGCGttacc    >  2:832783/1‑136 (MQ=255)

                                                                                                                                   taCCAGCCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTAccc    <  1:137933/140‑1 (MQ=255)

                                                                                                                                     ccagccagGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTTCCATGAGAAGTGAGCGCAGAGAGGGAATATTTTGCCTGGCTGAACTGGCGATTTAGTTTTCGCGGAccccg  >  2:1595519/1‑138 (MQ=255)

                                                                                                                                     ccagccagGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTTTTTTTCTGCCCTGAGAAGGGAGGGCAGAGAGGGGTTATTTTTCCTGACTGGACTGGCGATTAAATTTCCGCCTAGCCgg  >  2:146689/1‑140 (MQ=255)

                                                                                                                                     ccagccagGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg  <  2:64760/140‑1 (MQ=255)

                                                                                                                                     ccagccagGAGTCATATCAGGACGCAATGGCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCgg  <  1:996181/140‑1 (MQ=255)

TGCCGCAGAATGGAACACAGCCGAATTAAGAAGAGAAATGTCGCACTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAT‑TTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAGTTTCCGCGTACCCGG  >  NC_000913/4604151‑4604423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`