BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	542,669	T→C	L261P (CTC→CCC)	glxK →	glycerate 2‑kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	542,669	0	T	C	99.3%	483.8 / NA	137	L261P (CTC→CCC)	glxK	glycerate 2‑kinase 2
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (63/73); minor base A (0/1); total (63/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 140 total aligned reads displayed.

CCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGC  >  NC_000913/542533‑542803

ccGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTACGGGCGGGAT‑GAGTGTTGCGGCGGCGGTATTTCCCaa                                                                                                                                     >  1:874472/1‑140 (MQ=255)

   gagaTGGTCGAAGAACTTGAGCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  1:2086632/140‑1 (MQ=255)

   gagaTGGTCGAAGAACTTGAACGGGAGCTGCAAAATTCCGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  1:891659/140‑1 (MQ=255)

   gagaTGGTCGAAGAACTTGAACAGGGGCTTCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  1:945558/140‑1 (MQ=255)

   gagaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGTGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  1:555667/140‑1 (MQ=255)

   gagaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  2:51396/140‑1 (MQ=255)

   gagaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGc                                                                                                                                  <  1:227705/140‑1 (MQ=255)

     gaTGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCgg                                                                                                                                <  1:1645400/140‑1 (MQ=255)

           cGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGC‑GGCGGGATGGGGAATTGGGCGGGGGGAATTTCCCAATGGGGTAATTa                                                                                                                          >  1:1331438/1‑140 (MQ=255)

              agaaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTGCAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAAc                                                                                                                       <  2:1295583/140‑1 (MQ=255)

                aaCTTGAACAGGGGCTGTAAAATTACGCCCGCGTTCTACTACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCg                                                                                                                     <  2:767468/140‑1 (MQ=255)

                aaCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGAATTGCGGGGGCGGTATTTCCCAATGCGGATATTAAACCg                                                                                                                     >  2:863362/1‑140 (MQ=255)

                 aCTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCgg                                                                                                                    >  2:2111778/1‑140 (MQ=255)

                  cTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCggg                                                                                                                   <  1:174433/140‑1 (MQ=255)

                    tGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCa                                                                                                                 >  1:148862/1‑140 (MQ=255)

                     gAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCAt                                                                                                                <  2:2233828/140‑1 (MQ=255)

                       aCAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTg                                                                                                              <  1:491988/140‑1 (MQ=255)

                          ggggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGaaa                                                                                                           >  1:2067096/1‑140 (MQ=255)

                          ggggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGaaa                                                                                                           <  1:1584529/140‑1 (MQ=255)

                          ggggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGCATTTCCCAATGCCGATACTAAACCGGGCATTGaaa                                                                                                           >  2:821019/1‑140 (MQ=255)

                          ggggCTGCAAAATTAAGCCCGTTTTTTACTACAGCAATCTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGaaa                                                                                                           <  1:821019/140‑1 (MQ=255)

                           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGATATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCAt                                                                                                                <  2:384374/134‑1 (MQ=255)

                           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAAt                                                                                                          <  2:2476531/140‑1 (MQ=255)

                           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAAt                                                                                                          <  2:2121691/140‑1 (MQ=255)

                           gggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAAt                                                                                                          >  1:451333/1‑140 (MQ=255)

                            ggCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGAATTTCCCGATGAGGCTATTAACCCGGGCATTGAAAtt                                                                                                         >  1:700375/1‑140 (MQ=255)

                            ggCTGCAAAATTACGCCCGTGTTTTACAACAACAATCTGCAATTAATGCCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAAtt                                                                                                         <  2:808738/140‑1 (MQ=255)

                             gCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGTGGTGTGGGCGGTAT‑GGGGATTGCGGCGGCGGGATTTCCCAATGTGGATATTACACCGGGCGTTGCAATtg                                                                                                        >  2:1617080/1‑140 (MQ=255)

                               tGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATtgtg                                                                                                      <  2:454384/140‑1 (MQ=255)

                                gCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTgtgt                                                                                                     >  2:1943662/1‑140 (MQ=255)

                                     aTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                <  1:2288867/140‑1 (MQ=255)

                                     aTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                <  1:1758615/140‑1 (MQ=255)

                                     aTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                <  2:2389512/140‑1 (MQ=255)

                                     aTTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAAt                                                                                                <  2:78502/140‑1 (MQ=255)

                                      ttACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAAtt                                                                                                         >  2:2381488/1‑130 (MQ=255)

                                         cGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCgg                                                                                            >  1:531407/1‑140 (MQ=255)

                                          gCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGTGGt                                                                                           <  1:2111778/140‑1 (MQ=255)

                                           cccGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGt                                                                                           <  1:373376/139‑1 (MQ=255)

                                           cccGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTc                                                                                          >  1:358230/1‑140 (MQ=255)

                                              gtgtTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTATACCGGGCAGTGAAGTGGGGTCGAATGCGGGCagt                                                                                       >  2:2288867/1‑138 (MQ=255)

                                                gtTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTTAACCGGGGATTTAAATTGTGTTGAATGCGGTCAATCt                                                                                     >  1:812895/1‑140 (MQ=255)

                                                   ttACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGTGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgc                                                                                  <  1:1617080/140‑1 (MQ=255)

                                                     acaacaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTgcgc                                                                                <  2:358230/140‑1 (MQ=255)

                                                        acaGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcagg                                                                             <  2:1729891/140‑1 (MQ=255)

                                                         caGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggc                                                                            >  1:2361300/1‑140 (MQ=255)

                                                          aGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCACTGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggca                                                                           <  2:2206520/140‑1 (MQ=255)

                                                          aGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGGATTTCCCAATGCGGATATTAAACCGGGGATTTAGAGTGTGTTGAAAGCGGACAATCTTGCgcaggca                                                                           >  2:1123739/1‑140 (MQ=255)

                                                           gCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggcag                                                                          >  1:1347356/1‑140 (MQ=255)

                                                           gCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCgcaggcag                                                                          >  2:1651117/1‑140 (MQ=255)

                                                           gCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATAGAAATTGTGTTGAATGCGGTCTATCTTGCgcaggcag                                                                          >  1:1076967/1‑140 (MQ=255)

                                                            cAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTTAAATTGTGTTGTATGCGGTCACTCTTGCGCAGGCAGt                                                                         >  1:959883/1‑140 (MQ=255)

                                                               aCTGAAATTAATGTCTGCCAGATGGCGGGTGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCa                                                                      <  1:765897/140‑1 (MQ=255)

                                                               aCTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCa                                                                      <  2:531407/140‑1 (MQ=255)

                                                                    aaTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGgcg                                                                 <  2:181125/140‑1 (MQ=255)

                                                                    aaTTAATGTCTGCCAGATGGCGAGCGGCGCCGCTGCGTGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGgcg                                                                 <  1:960978/140‑1 (MQ=255)

                                                                     aTTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCAATGAAATTGCGTTGAATGCGGTCAATGTTGCACAGGCAGTGCAGGgcgc                                                                >  2:765897/1‑140 (MQ=255)

                                                                       tAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                              <  2:1313132/140‑1 (MQ=255)

                                                                         aTGTCTGCCAGATGGCGGGCGGCGGGGCTACGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcagca                                                            <  1:1012546/140‑1 (MQ=255)

                                                                         aTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcagca                                                            <  2:622346/140‑1 (MQ=255)

                                                                         aTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcagca                                                            <  2:1764805/140‑1 (MQ=255)

                                                                         aTGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcagca                                                            <  2:1295757/140‑1 (MQ=255)

                                                                          tGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCAc                                                           >  1:1795397/1‑140 (MQ=255)

                                                                             cTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                              >  2:389380/1‑134 (MQ=255)

                                                                             cTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                              <  1:389380/134‑1 (MQ=255)

                                                                             cTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCATGCACAAGCAGTGTAGGGCGCAGCACTgg                                                        >  1:644992/1‑140 (MQ=255)

                                                                              tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                              >  1:1959721/1‑133 (MQ=255)

                                                                              tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCgcag                                                              <  2:1959721/133‑1 (MQ=255)

                                                                              tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGt                                                       >  2:2460750/1‑140 (MQ=255)

                                                                              tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGt                                                       >  1:1424678/1‑140 (MQ=255)

                                                                              tGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGt                                                       <  2:1424678/140‑1 (MQ=255)

                                                                               gCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTg                                                      <  1:1287334/140‑1 (MQ=255)

                                                                                 cAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGAt                                                    <  2:56986/140‑1 (MQ=255)

                                                                                   gATGGCGGGCGGCGGCGCTGCGAGCGGTAT‑GGGTAGTGCAGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTa                                                  <  2:783623/140‑1 (MQ=255)

                                                                                    aTGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTAc                                                 <  1:2393097/140‑1 (MQ=255)

                                                                                        cgggcggCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGTGCAGGCATTGCAGGGCGCAGCACTGGTGATTACCggg                                             >  1:783623/1‑140 (MQ=255)

                                                                                          ggcggcggcGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGa                                           <  1:1654663/140‑1 (MQ=255)

                                                                                          ggcggcggcGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTCCAGGGCCCAGCACAGGTGATTACCGGGGa                                           >  1:1080114/1‑140 (MQ=255)

                                                                                              gcggcgCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAggg                                       >  2:2026958/1‑140 (MQ=255)

                                                                                                      gcgggcggTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGa                               <  2:2067096/140‑1 (MQ=255)

                                                                                                       cgggcggTAT‑GGGTATTGCGGCCGCGGTATTCCCCAATGCGGATATTACACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGAc                              <  2:690681/140‑1 (MQ=255)

                                                                                                          gcggTAT‑GGGTATTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCg                           <  1:55938/140‑1 (MQ=255)

                                                                                                                   ggTATTGCGGCGGCGGTATTTCCCAATGCGGGTATTAAATCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGc                   <  2:1055609/140‑1 (MQ=255)

                                                                                                                   ggTATTGCGGCGGCGGTATTTCACAATGCGGCTATTAAACCGGGCATTTAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGc                   <  2:1076967/140‑1 (MQ=255)

                                                                                                                      aTTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAgg                <  2:1264846/140‑1 (MQ=255)

                                                                                                                      aTTGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAgg                <  1:435677/140‑1 (MQ=255)

                                                                                                                        tGCGGCGGCGGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCg              >  2:196625/1‑140 (MQ=255)

                                                                                                                         gcggcggcgGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTGCCGGGGAGGGGGGCGTCGACTAGCAAACGGCAGGCgg             >  1:662131/1‑140 (MQ=255)

                                                                                                                         gcggcggcgGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCaaa                       <  2:600343/130‑1 (MQ=255)

                                                                                                                         gcggcggcgGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCaaa                       >  1:600343/1‑130 (MQ=255)

                                                                                                                         gcggcggcgGTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCgg             >  1:256305/1‑140 (MQ=255)

                                                                                                                          cggcggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGt            >  1:1816119/1‑140 (MQ=255)

                                                                                                                             cggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGGGCAACGACTCGCAGACGGCAGGCGGTaaa         >  1:684775/1‑140 (MQ=255)

                                                                                                                             cggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTaaa         >  1:319524/1‑140 (MQ=255)

                                                                                                                              ggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAACCGGCAGGCGGTAAAg        >  1:820052/1‑140 (MQ=255)

                                                                                                                              ggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAg        <  2:1347356/140‑1 (MQ=255)

                                                                                                                              ggcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAg        >  1:554909/1‑140 (MQ=255)

                                                                                                                               gcggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAgg       >  1:1265714/1‑139 (MQ=255)

                                                                                                                                cggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAgcg      <  2:451333/140‑1 (MQ=255)

                                                                                                                                cggTATTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGAGAATGGCCCATCGACTAGCAACTGGCAGGCGGTAAAgcg      >  2:1122221/1‑140 (MQ=255)

                                                                                                                                    aTTTCCCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGcgccgc  <  1:196625/140‑1 (MQ=255)

CCGGAGATGGTCGAAGAACTTGAACAGGGGCTGCAAAATTACGCCCGTGTTTTACAACAGCAAACTGAAATTAATGTCTGCCAGATGGCGGGCGGCGGCGCTGCGGGCGGTAT‑GGGTATTGCGGCGGCGGTATTTCTCAATGCGGATATTAAACCGGGCATTGAAATTGTGTTGAATGCGGTCAATCTTGCGCAGGCAGTGCAGGGCGCAGCACTGGTGATTACCGGGGAAGGGCGCATCGACTCGCAAACGGCAGGCGGTAAAGCGCCGC  >  NC_000913/542533‑542803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`