Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	731,989	T→G	S803A (TCA→GCA)	rhsC →	rhs element protein RhsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	731,989	0	T	G	100.0%	16.4 / NA	9	S803A (TCA→GCA)	rhsC	rhs element protein RhsC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/2);  total (7/2)

CATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCT  >  NC_000913/731854‑732017                                                                                                                                        |                             catcatCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAgg                          >  1:317276/1‑140 (MQ=255)  atcatcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGc                         >  2:2045551/1‑140 (MQ=255)  atcatcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGc                         >  2:2107161/1‑140 (MQ=255)      tcCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGa                     <  2:136442/140‑1 (MQ=11)        cGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCGGCCGTGGAGTGGTTGAGCTACGGCAGCGGTGACCTGGCAGGCATGaaa                   >  2:638573/1‑140 (MQ=11)           aGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTc                >  1:606246/1‑140 (MQ=255)             aCGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGCCCTACGGCAGCGGTAACCTGGCAGGCATGAAACTCgg              >  1:922234/1‑140 (MQ=11)             aCGAATGAACTGCCCCGGCAGCATGAGACCAGACATGCGAACAACGCACAGGGACGGGCGAACCGCTGTATACCGGACAGCCGGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGTTACCTGGCAGGCATGAAACTCgg              <  2:430771/140‑1 (MQ=11)                         ctctGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAACGGCTGCTTGGCAGGCCTGAAACTCGGCGACACACCGCt  >  2:1120573/1‑140 (MQ=11)                                                                                                                                        |                             CATCATCCGCAGACGAATGAACTGCTCTGGCAGCATGAGACCAGACATGCGTACAACGCACAGGGACTGGCGAACCGCTGTATACCGGACAGCCTGCCCGCCGTGGAATGGCTGACCTACGGCAGCGGCTGGCTGTCAGGCATGAAACTCGGCGACACACCGCT  >  NC_000913/731854‑732017

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑