BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	778,928	A→G	M397V (ATG→GTG)	tolB →	Tol‑Pal system periplasmic protein TolB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	778,928	0	A	G	100.0%	456.1 / NA	127	M397V (ATG→GTG)	tolB	Tol‑Pal system periplasmic protein TolB
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (63/64); total (63/64)

Only 100 of 132 total aligned reads displayed.

ATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTG  >  NC_000913/778794‑779064

aTGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGGCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGggggtggga                                                                                                                                     >  2:2064102/1‑140 (MQ=255)

 tggtggGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGggggtgggat                                                                                                                                    <  1:279620/140‑1 (MQ=255)

 tggtggGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGggggtgggat                                                                                                                                    <  2:2124396/140‑1 (MQ=255)

   gtggGCAGCAGCACATTGCCAAGCAAGATCCGGCATCGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATcc                                                                                                                                  <  2:1181361/140‑1 (MQ=255)

     gggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACGGCACTTCTCGGGGGGTGGGATCCGt                                                                                                                                >  1:829151/1‑140 (MQ=255)

     gggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGt                                                                                                                                >  1:228290/1‑140 (MQ=255)

     gggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGAAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGt                                                                                                                                <  1:993438/140‑1 (MQ=255)

      ggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACTTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTg                                                                                                                               >  1:605615/1‑140 (MQ=255)

      ggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTg                                                                                                                               >  2:2489940/1‑140 (MQ=255)

      ggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTg                                                                                                                               >  2:2136696/1‑140 (MQ=255)

      ggCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACGATGATAAGCTACAGCTTTTCTGAGGGGGTGGGATCCGTg                                                                                                                               >  2:763703/1‑140 (MQ=255)

            agcaCATTGCCAAACAAGATCTTGCCACGCGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAAt                                                                                                                         <  1:795572/140‑1 (MQ=255)

            agcaCATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAAt                                                                                                                         <  2:1325829/140‑1 (MQ=255)

               acaTTGCCAAACAAGCTCTGGCCACGGGACGCGTACACGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTg                                                                                                                      <  1:763703/140‑1 (MQ=255)

               acaTTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTg                                                                                                                      <  1:2489940/140‑1 (MQ=255)

                     ccAAACAAGATCTGTCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCt                                                                                                                >  2:457730/1‑140 (MQ=255)

                        aaCAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTg                                                                                                                      >  2:613404/1‑131 (MQ=255)

                        aaCAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTg                                                                                                                      <  1:613404/131‑1 (MQ=255)

                           aaGATCTGGCAACGGGAAGCGTCCAAGTTCTCTCGCCCACGTTCCTGGTTGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGAt                                                                                                          <  2:729847/140‑1 (MQ=255)

                               tCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGc                                                                                                      >  1:572326/1‑140 (MQ=255)

                                       cGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCaaa                                                                                              >  2:400554/1‑140 (MQ=255)

                                        gggAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAg                                                                                             >  1:381808/1‑140 (MQ=255)

                                        gggAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGAACCGCGCTGAATTTGGTGTCTACAGATGGGCGTTTCAAAg                                                                                             >  2:955233/1‑140 (MQ=255)

                                          gAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCTGTGGTGATTTTGGTTTGTACAGATGGGCGTTTCAAAgcg                                                                                           >  2:871701/1‑140 (MQ=255)

                                             gCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGt                                                                                        >  2:1403958/1‑140 (MQ=255)

                                             gCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGt                                                                                        >  1:1270548/1‑140 (MQ=255)

                                             gCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGAGCGTTTCAAAGCGCGt                                                                                        >  1:2341454/1‑140 (MQ=255)

                                                     gTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  1:2185527/140‑1 (MQ=255)

                                                     gTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  2:381808/140‑1 (MQ=255)

                                                     gTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  1:457730/140‑1 (MQ=255)

                                                     gTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  2:572326/140‑1 (MQ=255)

                                                     gTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  1:1011801/140‑1 (MQ=255)

                                                     gTTCTGTCGTCCACGCTCCTGGATGAAACTCCAAGTCAGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  1:1061462/140‑1 (MQ=255)

                                                          gtcgtcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTg                                                                           <  2:2270258/140‑1 (MQ=255)

                                                           tcgtcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGa                                                                          >  2:1856887/1‑140 (MQ=255)

                                                           tcgtcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGa                                                                          >  1:1651213/1‑140 (MQ=255)

                                                           tcgtcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGa                                                                          >  1:2083475/1‑140 (MQ=255)

                                                              tcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  2:1992680/131‑1 (MQ=255)

                                                              tcCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                >  1:1992680/1‑131 (MQ=255)

                                                                cACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGc                                                                                <  1:1988347/129‑1 (MQ=255)

                                                                  cGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAg                                                                   <  2:1651213/140‑1 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAg                                                                   <  1:1234468/127‑1 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAg                                                                   >  2:1234468/1‑127 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTcc                                                         >  1:2251473/1‑137 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCcctg                                                      >  2:2449433/1‑140 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCcctg                                                      >  2:310881/1‑140 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCcctg                                                      >  2:2268530/1‑140 (MQ=255)

                                                                               aaCGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCCTCCGGCAACTGATGGACAGGTCAAATTCcctg                                                      >  1:2270334/1‑140 (MQ=255)

                                                                                    cAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTgg                                                 >  1:1837456/1‑140 (MQ=255)

                                                                                    cAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCCTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTgg                                                 <  2:2270334/140‑1 (MQ=255)

                                                                                       gTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCg                                              <  1:400554/140‑1 (MQ=255)

                                                                                       gTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCg                                              <  2:1837456/140‑1 (MQ=255)

                                                                                        tCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGc                                             <  1:186872/140‑1 (MQ=255)

                                                                                          tGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACCGGCCAAATTCCCTGCCTGGTCGCCg                                           >  1:970821/1‑140 (MQ=255)

                                                                                             cACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTAt                                        <  1:59325/140‑1 (MQ=255)

                                                                                              aCCTAACGGCACTATGGTAATCTACAGCTCTTCTCTGGGCGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATc                                       <  2:895705/140‑1 (MQ=255)

                                                                                              aCCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACCGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATc                                       <  1:2136696/140‑1 (MQ=255)

                                                                                               ccTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCt                                      <  1:2268530/140‑1 (MQ=255)

                                                                                                  aaCGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCtgtg                                   >  2:246558/1‑140 (MQ=255)

                                                                                                   aCGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGa                                  <  2:1222249/140‑1 (MQ=255)

                                                                                                   aCGGCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGAGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGa                                  <  1:2064102/140‑1 (MQ=255)

                                                                                                     ggCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGata                                <  2:2344730/140‑1 (MQ=255)

                                                                                                      gCACTATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGataa                               >  1:1721405/1‑140 (MQ=255)

                                                                                                          tATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATaataat                           >  1:1784269/1‑140 (MQ=255)

                                                                                                          tATGGTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATaataat                           <  2:1270548/140‑1 (MQ=255)

                                                                                                             ggTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAAtaattaa                        <  2:2417555/140‑1 (MQ=255)

                                                                                                              gTAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGAGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAAtaattaat                       <  2:2341454/140‑1 (MQ=255)

                                                                                                               tAATCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCCGGTAGCCGTATCTGTGATAATaattaatt                      >  1:897094/1‑140 (MQ=255)

                                                                                                                 aTCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACTGGTCAAATTCCC‑GCCTGATCGCCGTATCTGTGATAATAAGTAATTGaa                   >  2:1138167/1‑140 (MQ=255)

                                                                                                                 aTCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGa                    >  1:1795265/1‑140 (MQ=255)

                                                                                                                  tCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGGGTTTCAAAGCGCGTCTTCCGGCAACTCATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  2:605615/140‑1 (MQ=255)

                                                                                                                  tCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  1:1731247/140‑1 (MQ=255)

                                                                                                                  tCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  2:1721405/140‑1 (MQ=255)

                                                                                                                  tCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  2:1997745/140‑1 (MQ=255)

                                                                                                                  tCTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  1:2449433/140‑1 (MQ=255)

                                                                                                                   cTACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAAt                  <  1:246558/140‑1 (MQ=255)

                                                                                                                   cTACAGCTCTTCTCAAGGGGTGTGAGCCGCGCTGAATATGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAAt                  <  1:809807/140‑1 (MQ=255)

                                                                                                                    tACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATa                 <  1:310881/140‑1 (MQ=255)

                                                                                                                    tACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATa                 >  2:479775/1‑140 (MQ=255)

                                                                                                                    tACAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATa                 >  2:63561/1‑140 (MQ=255)

                                                                                                                      cAGCTTTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  1:353249/136‑1 (MQ=255)

                                                                                                                      cAGCTTTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   >  2:353249/1‑136 (MQ=255)

                                                                                                                      cAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCGAATTCCCTGCCTGGTCGCCGTATCTATGATGATAATTAATTGAATAGt               >  2:642444/1‑140 (MQ=255)

                                                                                                                      cAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCGTGGTCGCCGTAACTCTGATAATAATTAATTGAATAGt               >  1:1114776/1‑140 (MQ=255)

                                                                                                                      cAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGt               >  1:1752617/1‑140 (MQ=255)

                                                                                                                      cAGCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGt               >  2:2088924/1‑140 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTg                     <  1:1301403/132‑1 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTg                     >  2:1301403/1‑132 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   >  2:2309480/1‑134 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGaa                   <  1:2309480/134‑1 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTaa             >  2:2474370/1‑140 (MQ=255)

                                                                                                                        gCTCTTCTCAGGGGGTGGGATCAGTTCTGCATTTGGTGTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTaa             <  1:638608/140‑1 (MQ=255)

                                                                                                                         gacagctcAGGGGGTGGGATCCCTGTTGAATTTGGTTCCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTaaa            <  1:949358/135‑1 (MQ=255)

                                                                                                                            ttctCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTg                     >  1:1631489/1‑128 (MQ=255)

                                                                                                                            ttctCAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGa         >  1:1303568/1‑140 (MQ=255)

                                                                                                                             tctcAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGaa        >  2:195540/1‑140 (MQ=255)

                                                                                                                             tctcAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGaa        >  1:2453044/1‑140 (MQ=255)

                                                                                                                              ctcAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAAtaattaa                        <  1:1650450/123‑1 (MQ=255)

                                                                                                                              ctcAGGGGGTGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAAtaattaa                        >  2:1650450/1‑123 (MQ=255)

                                                                                                                                   ggggtgggatCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTg  <  2:2453044/140‑1 (MQ=255)

ATGGTGGGCAGCAGCACATTGCCAAACAAGATCTGGCAACGGGAGGCGTACAAGTTCTGTCGTCCACGTTCCTGGATGAAACGCCAAGTCTGGCACCTAACGGCACTATGGTAATCTACAGCTCTTCTCAGGGGATGGGATCCGTGCTGAATTTGGTTTCTACAGATGGGCGTTTCAAAGCGCGTCTTCCGGCAACTGATGGACAGGTCAAATTCCCTGCCTGGTCGCCGTATCTGTGATAATAATTAATTGAATAGTAAAGGAATCATTG  >  NC_000913/778794‑779064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`