BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	832,792	T→C	A53A (GCA→GCG)	ybiA ←	N‑glycosidase YbiA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	832,792	0	T	C	99.4%	579.2 / NA	163	A53A (GCA→GCG)	ybiA	N‑glycosidase YbiA
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (78/84); total (78/85)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAC  >  NC_000913/832655‑832920

 ttCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGTGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGcc                                                                                                                               <  2:2071603/140‑1 (MQ=255)

  tCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCt                                                                                                                              <  2:2334037/140‑1 (MQ=255)

       tCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGaaaa                                                                                                                         <  1:179568/140‑1 (MQ=255)

       accccTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGaaaa                                                                                                                         <  2:1538850/137‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  2:253142/140‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  1:1921178/140‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  2:2126336/140‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  1:1315766/140‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  1:436269/140‑1 (MQ=255)

         aCCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATa                                                                                                                       <  2:1387832/140‑1 (MQ=255)

          ccagTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAg                                                                                                                      <  2:1491952/137‑1 (MQ=255)

          ccTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAg                                                                                                                      <  1:560443/140‑1 (MQ=255)

           cTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAgt                                                                                                                     >  1:1511913/1‑140 (MQ=255)

              ttCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGtt                                                                                                                  >  2:1858045/1‑140 (MQ=255)

               tCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTc                                                                                                                 >  2:1259314/1‑140 (MQ=255)

                     aCCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGt                                                                                                           >  2:1975658/1‑140 (MQ=255)

                        gACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAgg                                                                                                        >  1:1864436/1‑140 (MQ=255)

                        gACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAgg                                                                                                        >  1:244628/1‑140 (MQ=255)

                        gACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAgg                                                                                                        >  2:350450/1‑140 (MQ=255)

                         aCTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGc                                                                                                       >  1:1592595/1‑140 (MQ=255)

                         aCTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTGGCGGCTGAAAATAGTGTTCTGAGGTAGGc                                                                                                       >  1:650274/1‑140 (MQ=255)

                               aGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGc                                                                                                       >  2:2318739/1‑134 (MQ=255)

                               aGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGc                                                                                                       <  1:2318739/134‑1 (MQ=255)

                               aGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGCtt                                                                                                 <  2:1592595/140‑1 (MQ=255)

                                      aCGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGt                                                                                          <  2:244628/140‑1 (MQ=255)

                                           gagGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGCAAAAATTATTCTGAGGAAGGCGTGGTTTTCCCGTCAAcc                                                                                     >  2:1103676/1‑140 (MQ=255)

                                           gagGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAcc                                                                                     >  1:2156652/1‑140 (MQ=255)

                                           gagGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAcc                                                                                     >  1:1533897/1‑140 (MQ=255)

                                           gagGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAcc                                                                                     >  2:1644038/1‑140 (MQ=255)

                                           gagGCTTAAAACGATCGAGCCCCATGCGTGTGGCGTCCATAGGGGAAGTAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGCAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAAcc                                                                                     <  2:1216013/140‑1 (MQ=255)

                                                 tAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATg                                                                               <  2:2088583/140‑1 (MQ=255)

                                                      cGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCa                                                                          <  1:1893000/140‑1 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTTAAAATAGTGTTCTGAGGTAGGTCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  1:257189/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  1:2139796/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  2:1644070/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  2:1774905/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  1:1411416/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  1:1234851/1‑140 (MQ=255)

                                                        aTCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGCATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAgg                                                                        >  2:2140604/1‑140 (MQ=255)

                                                         tCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAggc                                                                       <  2:2001441/140‑1 (MQ=255)

                                                           gcgcCCCATGCGTGCGGCGACCATAGGGGTAGAAACCTGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAggcgg                                                                     <  1:1082125/140‑1 (MQ=255)

                                                             gcCCCATGCGTGCGGGGACCATTGGCGAAAAAACCCGCCGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCa                                                                   <  1:1016776/140‑1 (MQ=255)

                                                              ccccATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCaa                                                                  >  1:567672/1‑140 (MQ=255)

                                                              ccccATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCaa                                                                  <  1:2204743/140‑1 (MQ=255)

                                                              ccccATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCaa                                                                  >  1:2148064/1‑140 (MQ=255)

                                                              ccccATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCaa                                                                  <  1:1644038/140‑1 (MQ=255)

                                                                   tgcgtgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCaa                                                                  <  2:1387790/135‑1 (MQ=255)

                                                                   tgcgtgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTa                                                             >  2:2489171/1‑140 (MQ=255)

                                                                   tgcgtgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTa                                                             >  2:292532/1‑140 (MQ=255)

                                                                   tgcgtgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGACAATCTTGATGGGACAGGCGGCAAAGTTa                                                             >  1:1011087/1‑140 (MQ=255)

                                                                       tgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTGCCGTCAAGCTTCATCGGCCAGGCGGCAAAGTTAGaaa                                                         >  1:794015/1‑140 (MQ=255)

                                                                       tgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaa                                                         >  2:2153179/1‑140 (MQ=255)

                                                                       tgcgGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaa                                                         <  2:57117/140‑1 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaaaa                                                       >  2:1711217/1‑140 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaaaa                                                       <  1:1259314/140‑1 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGaaaaa                                                       >  2:488386/1‑140 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGAAGGGCCAGGCGGCAAAGTTAGaaaaa                                                       >  1:973961/1‑140 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGACAACATTGATGGTCCAGACGGCAAAGTTAGaaaaa                                                       >  2:1168536/1‑140 (MQ=255)

                                                                         cggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCAGTCAACAGTGAGGGACCAGGCGGCAAAGTTAGaaaaa                                                       >  1:639452/1‑140 (MQ=255)

                                                                          ggcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAAt                                                      <  1:139939/140‑1 (MQ=255)

                                                                          ggcgACCATAGGGGAAGAAACCCGACGGATCACTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAAt                                                      <  2:1055139/140‑1 (MQ=255)

                                                                           gcgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCGGGTTTTCCGGTCAACCATGATGGGCGAGGCCGCAAAGTTAGAAAAATc                                                     >  2:732889/1‑140 (MQ=255)

                                                                            cgACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCa                                                    <  2:1303011/140‑1 (MQ=255)

                                                                              aCCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCAcc                                                  >  2:2191400/1‑140 (MQ=255)

                                                                                    ggggAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTc                                            <  2:1684469/140‑1 (MQ=255)

                                                                                     gggAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCa                                           >  1:2409419/1‑140 (MQ=255)

                                                                                         agaaACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTTAAAATAGTGTTCTGAGGTAGGTCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGc                                       <  2:257189/140‑1 (MQ=255)

                                                                                          gaaACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCt                                      >  2:562976/1‑140 (MQ=255)

                                                                                             aCCCGACGGATCTCTTCGCGGTATTTATCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGACGGCAAATTTAAAAGAATCACAGTAGTCATCGCTGGt                                   >  1:732005/1‑140 (MQ=255)

                                                                                              cccGACGGATCTCTTCGGGGTACTTTTCATCCAGGTATTCTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg                                  <  2:650274/140‑1 (MQ=255)

                                                                                              cccGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg                                  <  2:2432810/140‑1 (MQ=255)

                                                                                              cccGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg                                  <  2:1870184/140‑1 (MQ=255)

                                                                                              cccGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg                                  <  1:2446599/140‑1 (MQ=255)

                                                                                              cccGACGGATCTCTTCGCGGTACTTTTCAGCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTg                                  <  1:849074/140‑1 (MQ=255)

                                                                                               ccGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGc                                 >  1:2236835/1‑140 (MQ=255)

                                                                                               ccGACGGATCTCTTCGCGGTATTTTGCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGc                                 <  1:1045962/140‑1 (MQ=255)

                                                                                               ccGACGGATCTCTTCGCAGTATCTTTCATCAGGGAATTTTTGCGCCTGCAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGc                                 <  2:973961/140‑1 (MQ=255)

                                                                                                  aCGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTg                                     <  2:1824251/133‑1 (MQ=255)

                                                                                                   cGGATCTCTTCGCGGCATTTTTCATCAAGCAATTTTTACGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTa                             <  1:1148140/140‑1 (MQ=255)

                                                                                                      aTCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGaa                          <  2:125883/140‑1 (MQ=255)

                                                                                                           ttCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTa                     <  2:1511913/140‑1 (MQ=255)

                                                                                                           ttCGCGGTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTa                     <  1:1644070/140‑1 (MQ=255)

                                                                                                                ggTATTTTTCATCAAGGTATTTTTGCGCCTGCAAATAGTCTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg                <  2:647061/140‑1 (MQ=255)

                                                                                                                ggTATTTTTCATCAAGGTATTTCTGCGCCTGAAAATAGTTTTCTGAGGGAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg                <  2:831721/140‑1 (MQ=255)

                                                                                                                ggTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg                <  2:2409419/140‑1 (MQ=255)

                                                                                                                ggTATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCAACGTAGTCATCGCGGGTGTTGTAGAAATTTatgatg                >  2:813155/1‑140 (MQ=255)

                                                                                                                  tATTTTTCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGgt              >  2:2151861/1‑140 (MQ=255)

                                                                                                                     ttttCATCAAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTc           >  1:1850917/1‑140 (MQ=255)

                                                                                                                        tcatcaAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTg        <  1:1975658/140‑1 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATGGCTGGTGATGTAGAGATTTATTATGGTGTCTTGCAtc    >  1:831721/1‑139 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg                <  2:2463534/128‑1 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTatgatg                >  1:2463534/1‑128 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATg    >  1:1848941/1‑140 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATg    >  2:2093503/1‑140 (MQ=255)

                                                                                                                            caAGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATg    >  2:1976357/1‑140 (MQ=255)

                                                                                                                              aGGAATTTTTGCGCCTGAAAATGGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc  <  1:1136066/140‑1 (MQ=255)

                                                                                                                              aGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTTTAGAAATTTATGATGGTGTCTTGCATGAc  >  1:1111043/1‑140 (MQ=255)

                                                                                                                              aGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc  <  2:107424/140‑1 (MQ=255)

                                                                                                                              aGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc  >  1:1260972/1‑140 (MQ=255)

                                                                                                                              aGGAATTTTTGCGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAc  <  1:2111214/140‑1 (MQ=255)

TTTCGCATCACCTGTTCTTTGACCGACTCCCAGTTTTTACGCAGAGGCTTAGAACGATCGCGCCCCATGCGTGCGGCGACCATAGGGGAAGAAACCCGACGGATCTCTTCGCGGTATTTTTCATCAAGGAATTTTTGTGCCTGAAAATAGTGTTCTGAGGTAGGCCAGGTTTTCCCGTCAACCTTGATGGGCCAGGCGGCAAAGTTAGAAAAATCACCGTAGTCATCGCTGGTGCTGTAGAAATTTATGATGGTGTCTTGCATGAC  >  NC_000913/832655‑832920

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`