Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	950,923	A→G	V53A (GTT→GCT)	pflA ←	pyruvate formate‑lyase activating enzyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	950,923	0	A	G	100.0%	338.3 / NA	96	V53A (GTT→GCT)	pflA	pyruvate formate‑lyase activating enzyme
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (41/55);  total (41/55)

CTTCTTTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGG  >  NC_000913/950786‑951055                                                                                                                                          |                                                                                                                                     cttcttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCg                                                                                                                                    >  2:2193819/1‑140 (MQ=255) cttcttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCg                                                                                                                                    >  2:121612/1‑140 (MQ=255) cttcttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCg                                                                                                                                    >  1:2085423/1‑140 (MQ=255) cttcttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCg                                                                                                                                    >  2:1966374/1‑140 (MQ=255)  ttcttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCgg                                                                                                                                   >  2:1718856/1‑140 (MQ=255)   tcttTTTTGCAGGCGCGGAACCTGTCACGTACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGt                                                                                                                                  <  1:1214986/140‑1 (MQ=255)    cttTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTa                                                                                                                                 <  1:1658957/140‑1 (MQ=255)      tttttGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAAc                                                                                                                               <  2:1390251/140‑1 (MQ=255)            cAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCttt                                                                                                                         <  1:2209659/140‑1 (MQ=255)                 gcgGAACCAGTCACGTACAAACTCAGCTTACAGGATTCCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAATTCTTCAGCGGTAACTTCTTTACCGc                                                                                                                    <  1:858230/140‑1 (MQ=255)                 gcgGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTGCCGc                                                                                                                    <  2:1427533/140‑1 (MQ=255)                 gcgGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGc                                                                                                                    <  2:1932104/140‑1 (MQ=255)                     aaCCAGTCACGAACACACTCAGTTTTCAGTATTCCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATg                                                                                                                <  1:779701/140‑1 (MQ=255)                     aaCCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATg                                                                                                                <  1:1853927/140‑1 (MQ=255)                         aGTCACGAACAAACTCGGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATg                                                                                                                >  1:548678/1‑136 (MQ=255)                         aGTCACGAACAAACTCGGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATg                                                                                                                <  2:548678/136‑1 (MQ=255)                           tCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCATTTCCTTCATCAAGTCTGCAGCGGTACCTTCGTTACCGCCTTGCGTGTc                                                                                                          >  2:1187780/1‑140 (MQ=255)                           tCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCAATTCCTTCACCAATTCTACAGCGGTATCTTCGTTACCGCCATGCGTGTc                                                                                                          >  2:779701/1‑140 (MQ=255)                             aCGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTccc                                                                                                        <  1:2340491/140‑1 (MQ=255)                             aCGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCACCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTccc                                                                                                        <  2:1383843/140‑1 (MQ=255)                                aaCAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAgg                                                                                                     >  2:64482/1‑140 (MQ=255)                                    aaCTCAGCTTGTAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCCTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTc                                                                                                 <  2:82147/140‑1 (MQ=255)                                    aaCTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTc                                                                                                 <  2:600080/140‑1 (MQ=255)                                     aCTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTcg                                                                                                <  2:2232540/140‑1 (MQ=255)                                     aCTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTcg                                                                                                <  1:1377622/140‑1 (MQ=255)                                       tCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTcgcg                                                                                              <  2:1269791/140‑1 (MQ=255)                                         aGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGt                                                                                            <  1:1271735/140‑1 (MQ=255)                                         aGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGt                                                                                            <  2:214365/140‑1 (MQ=255)                                         aGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGt                                                                                            >  2:788642/1‑140 (MQ=255)                                           cTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTa                                                                                          >  2:2441936/1‑140 (MQ=255)                                            ttGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTAt                                                                                         >  1:1736025/1‑140 (MQ=255)                                             tGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATg                                                                                        <  1:2173286/140‑1 (MQ=255)                                             tGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATg                                                                                        <  1:1915761/140‑1 (MQ=255)                                             tGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATg                                                                                        <  2:1385462/140‑1 (MQ=255)                                                aGGATTGCTTCACCGTCGGATGTGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACa                                                                                     <  1:837335/140‑1 (MQ=255)                                                aGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACa                                                                                     >  2:389557/1‑140 (MQ=255)                                                     tGCTTCACCGCCGGATGCGGTATCGTCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGAGGTTATGACAATACa                                                                                <  1:774669/140‑1 (MQ=255)                                                         tCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCa                                                                            <  1:1426769/140‑1 (MQ=255)                                                            ccgccgGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATTCGTATCCAAGGTGTCGTGGTTTTGACAATACAGGCAgcg                                                                         >  2:1026886/1‑140 (MQ=255)                                                            ccgccgGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATACGTTTCCTAGGTGTCGTGGTTATGACAATACAGGCAgcg                                                                         >  2:1098258/1‑140 (MQ=255)                                                              gccgGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCa                                                                       >  1:1997818/1‑140 (MQ=255)                                                               ccgGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCAt                                                                      >  2:2039881/1‑140 (MQ=255)                                                                   aTGCGGTAACGCCGCTGCCGGAAGCATTCCTAAAGTGCCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAgg                                                                  <  1:891360/140‑1 (MQ=255)                                                                   aTGCGGTAACGCCGCCGTCGGACGCGTTCATAAAGTGGCCATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAgg                                                                  <  2:824263/140‑1 (MQ=255)                                                                   aTGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAgg                                                                  <  2:504630/140‑1 (MQ=255)                                                                     gCGGTAACGCCGCCGACGGAAGGGTTCATAAAGTGGCTATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCa                                                                <  1:968729/140‑1 (MQ=255)                                                                        gTAACGCGGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGcc                                                             <  2:2085423/140‑1 (MQ=255)                                                                        gTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGcc                                                             <  2:1928812/140‑1 (MQ=255)                                                                        gTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGcc                                                             <  2:1286908/140‑1 (MQ=255)                                                                                       gAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGAt                                              <  1:81247/140‑1 (MQ=255)                                                                                        aaGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATa                                             <  2:1997818/140‑1 (MQ=255)                                                                                            gTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCAACAGACAGCCCTGGAAAAAGGTGATAAAGc                                         >  1:688174/1‑140 (MQ=255)                                                                                             ttCATAAAGTGGCGATAGGTCATCATTTCATTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCg                                        <  1:1187780/140‑1 (MQ=255)                                                                                             ttCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCg                                        <  1:1222840/140‑1 (MQ=255)                                                                                             ttCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCg                                        <  2:1404395/140‑1 (MQ=255)                                                                                             ttCATAAAGTGGCGATACGTCATCACTTCCTCCATCAAACCTTCAGCGCTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCg                                        <  2:688174/140‑1 (MQ=255)                                                                                                    aGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCt                                 >  2:2269210/1‑140 (MQ=255)                                                                                                       ggCGATAGGTCACCACTTCCTTCATCAAAACTTCAGCTGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTggg                              <  1:1026886/140‑1 (MQ=255)                                                                                                        gCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGc                             >  2:399748/1‑140 (MQ=255)                                                                                                        gCGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGACAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGc                             >  1:787768/1‑140 (MQ=255)                                                                                                         cGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGcc                            <  1:388279/140‑1 (MQ=255)                                                                                                         cGATAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGCAAAGAGGTGATAAAGCGAATACCTGGGcc                            >  1:835005/1‑140 (MQ=255)                                                                                                            tAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTc                         <  1:2193819/140‑1 (MQ=255)                                                                                                            tAGGTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTc                         <  1:1247317/140‑1 (MQ=255)                                                                                                              ggTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTa                       >  1:2178210/1‑140 (MQ=255)                                                                                                              ggTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTa                       <  1:64482/140‑1 (MQ=255)                                                                                                               gTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTgg                               >  1:1972331/1‑131 (MQ=255)                                                                                                               gTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTgg                               <  2:1972331/131‑1 (MQ=255)                                                                                                               gTCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCAACAGGCAGCGCTGGAAATAGGTGATAAAGCGAATACCTGGGCCGTCTAc                      >  2:846960/1‑140 (MQ=255)                                                                                                                tCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACg                     >  1:2157044/1‑140 (MQ=255)                                                                                                                tCACCACTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACg                     >  2:278417/1‑140 (MQ=255)                                                                                                                  accacTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGt                   >  2:1657485/1‑140 (MQ=255)                                                                                                                  accacTTCCTTCATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGt                   >  1:126557/1‑140 (MQ=255)                                                                                                                      cttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  1:389557/140‑1 (MQ=255)                                                                                                                      cttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  1:2441936/140‑1 (MQ=255)                                                                                                                      cttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  2:2295389/140‑1 (MQ=255)                                                                                                                      cttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  1:2269210/140‑1 (MQ=255)                                                                                                                      cttccttcATCAAATCTTCAGCAGTCACTCCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  1:788642/140‑1 (MQ=255)                                                                                                                      cttccttcATCAAATCTACAGCAGTAACTTCTTTATCGCAATGCGTGTACCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCAAATACCTGGGCCGTCTACGGTTCca               <  2:787768/140‑1 (MQ=255)                                                                                                                       ttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCcac              >  1:1931088/1‑140 (MQ=255)                                                                                                                       ttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               <  2:2242693/139‑1 (MQ=255)                                                                                                                       ttccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCca               >  1:2242693/1‑139 (MQ=255)                                                                                                                        tccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCGTGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCcaca             >  1:2254497/1‑140 (MQ=255)                                                                                                                        tccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCcaca             <  1:2469506/140‑1 (MQ=255)                                                                                                                        tccttcATCAAATCTCCAGCGGGAATTTCGTTACCGCAATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCcaca             <  1:1091463/140‑1 (MQ=255)                                                                                                                         ccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTa                       >  1:2382055/1‑129 (MQ=255)                                                                                                                         ccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTa                       <  2:2382055/129‑1 (MQ=255)                                                                                                                         ccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAg            >  1:227028/1‑140 (MQ=255)                                                                                                                         ccttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGCGATAAAGAGAAAACCTGGGCCCTCTACGGTTCCACAg            >  1:1063522/1‑140 (MQ=255)                                                                                                                          cttcATCAAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCCGGGCCGTCTACGGTTCCACAgg           >  2:1133557/1‑140 (MQ=255)                                                                                                                          cttcATCAAATCTTCAACGGTATCTTCTTTAACGCCATGTGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAgg           <  2:1151540/140‑1 (MQ=255)                                                                                                                              atcaAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTc       >  2:1651562/1‑140 (MQ=255)                                                                                                                              atcaAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTAGGACGTCTATGGTTCCACAGGATTc       >  1:1151540/1‑140 (MQ=255)                                                                                                                              atcaAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGAAAAAGCTAATACCTGGGACGTCTACGGTTCCCCAGGATTc       >  2:1119589/1‑140 (MQ=255)                                                                                                                                caAATCTTCAGCGGTTACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCaa     <  1:1098258/140‑1 (MQ=255)                                                                                                                                caAATCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCaa     >  1:1901717/1‑140 (MQ=255)                                                                                                                                caAATCTTCAGCGGTAACTTCTCTACCGCCATGCGTGCCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCaa     <  1:1119589/140‑1 (MQ=255)                                                                                                                                   aTCTTCAGCGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAgg  >  2:2312530/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     CTTCTTTTTTGCAGGCGCGGAACCAGTCACGAACAAACTCAGCTTGCAGGATTGCTTCACCGCCGGATGCGGTAACGCCGCCGCCGGAAGCGTTCATAAAGTGGCGATAGGTCACCACTTCCTTCATCAAATCTTCAACGGTAACTTCTTTACCGCCATGCGTGTCCCAGGTGTCGCGGTTATGACAATACAGGCAGCGCATCAGGCAGCCCTGGAAAAAGGTGATAAAGCGAATACCTGGGCCGTCTACGGTTCCACAGGATTCAAAGG  >  NC_000913/950786‑951055

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑