BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,048,907	A→G	S142S (AGT→AGC)	gfcB ←	lipoprotein GfcB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,048,907	0	A	G	98.8%	571.0 / NA	162	S142S (AGT→AGC)	gfcB	lipoprotein GfcB
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (89/70); minor base T (0/2); total (89/73)
Fisher's exact test for biased strand distribution p-value = 1.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 165 total aligned reads displayed.

TAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATT  >  NC_000913/1048771‑1049041

  gCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAg                                                                                                                                   >  1:343098/1‑140 (MQ=255)

          gACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCt                                                                                                                                      >  1:426547/1‑129 (MQ=255)

          gACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCag                                                                                                                           >  1:2470564/1‑140 (MQ=255)

             ccAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGc                                                                                                                        <  1:1309888/140‑1 (MQ=255)

               aGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGa                                                                                                                      <  2:343098/140‑1 (MQ=255)

                     tgtTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATgg                                                                                                                <  2:221276/140‑1 (MQ=255)

                      gtTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGt                                                                                                               >  2:491962/1‑140 (MQ=255)

                       ttttGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGtt                                                                                                              <  1:1486072/140‑1 (MQ=255)

                       ttttGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAACGGtt                                                                                                              >  2:1464643/1‑140 (MQ=255)

                        tttGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTg                                                                                                             >  2:109606/1‑140 (MQ=255)

                         ttGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGt                                                                                                            <  1:109606/140‑1 (MQ=255)

                         ttGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGt                                                                                                            >  2:523325/1‑140 (MQ=255)

                           gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTGAGCG‑‑TCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTc                                                                                                        >  2:2350190/1‑140 (MQ=255)

                           gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCt                                                                                                          >  2:2059382/1‑140 (MQ=255)

                           gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCt                                                                                                          >  2:1324574/1‑140 (MQ=255)

                           gCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCACGGCCGCAGATCGAAAATGGTTGTCt                                                                                                          >  1:1045147/1‑140 (MQ=255)

                             cACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTc                                                                                                        <  2:2318624/140‑1 (MQ=255)

                                tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                     <  1:51431/140‑1 (MQ=255)

                                tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                     <  2:2212012/140‑1 (MQ=255)

                                tGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                     <  2:1355181/140‑1 (MQ=255)

                                   ttttCTGGCCGCGTGGAAGTCATCTCTTCCTTCCACACATTACACAGCTCATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCa                                                                                                  <  2:923532/140‑1 (MQ=255)

                                    tttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCag                                                                                                 <  1:1660124/140‑1 (MQ=255)

                                     ttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGGAGATCGGAAATGGGTGTCTTCACTCCaga                                                                                                >  1:923532/1‑140 (MQ=255)

                                     ttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCa                                                                                                  <  2:584049/138‑1 (MQ=255)

                                     ttCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCa                                                                                                  >  1:584049/1‑138 (MQ=255)

                                          gCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAata                                                                                           >  2:2075393/1‑140 (MQ=255)

                                          gCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATAGTTGTCTTCACTCCAGAGAata                                                                                           >  2:751953/1‑140 (MQ=255)

                                            cgcgTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGGAAACGGGTGTCTTTACTCCAGAGAATATc                                                                                         >  1:946107/1‑140 (MQ=255)

                                            cgcgTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACt                                                                                                     >  2:1852722/1‑128 (MQ=255)

                                              cgTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGTGAAAATGGTTCTCTTCCCTCCAGAGAATATcgc                                                                                       >  2:1111538/1‑140 (MQ=255)

                                                       cACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTGGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGc                                                                              <  1:1745576/140‑1 (MQ=255)

                                                        aCCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCg                                                                             >  2:462151/1‑140 (MQ=255)

                                                            ctTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTg                                                                         <  2:2470564/140‑1 (MQ=255)

                                                             ttCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGc                                                                        >  1:1682560/1‑140 (MQ=255)

                                                             ttCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTCTCTTCACACCAGAGAATATCGTGCGTCCAGCGGCTGc                                                                        >  1:853372/1‑140 (MQ=255)

                                                              tCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTGAGCG‑‑TCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                     <  1:2350190/140‑1 (MQ=255)

                                                               ccTGCCAGACATTACACAGCACATTGCGACCCACAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCCGCCa                                                                      >  2:1729065/1‑140 (MQ=255)

                                                                cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGcc                                                                       >  2:1594942/1‑138 (MQ=255)

                                                                cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGcc                                                                       <  1:1594942/138‑1 (MQ=255)

                                                                cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                     <  1:2059382/140‑1 (MQ=255)

                                                                cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                     <  2:1682560/140‑1 (MQ=255)

                                                                cTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                     <  2:396296/140‑1 (MQ=255)

                                                                  gCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCt                                                                   >  1:1885698/1‑140 (MQ=255)

                                                                    cAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAg                                                                               <  2:1633696/126‑1 (MQ=255)

                                                                            acacAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCa                                                         >  1:105248/1‑140 (MQ=255)

                                                                              acaGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAgc                                                       <  2:1874484/140‑1 (MQ=255)

                                                                                 gCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATCTCGCGCGTCCAGCGGCAGCCACCTTTTATTGCCAGcgcg                                                    >  2:807947/1‑140 (MQ=255)

                                                                                 gCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCa                                                         <  1:274438/135‑1 (MQ=255)

                                                                                 gCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGcgcg                                                    <  1:751953/140‑1 (MQ=255)

                                                                                 gCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGcgcg                                                    >  1:1619334/1‑140 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCAt                                                                     <  1:2368351/122‑1 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                   >  1:24495/1‑140 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                   >  2:2006045/1‑140 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                   >  1:146975/1‑140 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGt                                                   >  1:472906/1‑140 (MQ=255)

                                                                                  cacaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCAAGAGAACAGCGCTCGTGCAGCGGCTGCCATATTTTATTGCCAGCGCGt                                                   >  2:637558/1‑140 (MQ=255)

                                                                                   acaTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGtt                                                  >  2:2270852/1‑140 (MQ=255)

                                                                                     aTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                                >  2:313496/1‑140 (MQ=255)

                                                                                     aTTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTAc                                                >  1:387374/1‑140 (MQ=255)

                                                                                       tGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGGATATCGCGAGTCCAGCGGCTGCCATATTTTATTGCCAGCGCGTTACGc                                              >  2:952192/1‑140 (MQ=255)

                                                                                       tGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGc                                              >  2:1961834/1‑140 (MQ=255)

                                                                                       tGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGATGCTATCTTTTATTGCCAGCGCGTTACGc                                              >  1:928271/1‑140 (MQ=255)

                                                                                         cGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTACCAGCTGTTATTGCCAGCGAGTTACGCag                                            >  1:1133176/1‑140 (MQ=255)

                                                                                          gACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCaga                                           <  1:2302213/140‑1 (MQ=255)

                                                                                            cccGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAgg                                         >  2:2483353/1‑140 (MQ=255)

                                                                                            cccGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAgg                                         >  2:1311849/1‑140 (MQ=255)

                                                                                             ccGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAggg                                        >  1:61254/1‑140 (MQ=255)

                                                                                               gCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGGTGCCATCGTTTATTGCCAGCGAGTTACGCAGAGGGTc                                      >  1:638494/1‑140 (MQ=255)

                                                                                               gCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                      <  2:1712698/140‑1 (MQ=255)

                                                                                               gCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                      <  1:1423372/140‑1 (MQ=255)

                                                                                               gCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGACGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                      >  2:2134985/1‑140 (MQ=255)

                                                                                                cAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGGGTCCAGCGGCTGCCATGTTTTATTGGCAGCTCGTTACGCAGAGGGTcc                                     >  2:728166/1‑140 (MQ=255)

                                                                                                cAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTcc                                     >  2:1438820/1‑140 (MQ=255)

                                                                                                    atTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGcc                                 <  2:1310380/140‑1 (MQ=255)

                                                                                                    atTCAGCGTCTCTAATCCAGCAAAGGAAAAAATAGGGCTCAGGGTCGCTGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGcc                                 <  2:853372/140‑1 (MQ=255)

                                                                                                          cGTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAAt                           >  1:2053746/1‑140 (MQ=255)

                                                                                                           gTCTCTAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAAtt                          >  2:1374708/1‑140 (MQ=255)

                                                                                                             ctctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGTCATCTTTTATTGACAGCGCGTTACGTAGAGTGTCCTGCCCGGAATTTg                        >  2:798928/1‑140 (MQ=255)

                                                                                                             ctctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                      >  2:2327170/1‑126 (MQ=255)

                                                                                                             ctctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTc                                      <  1:2327170/126‑1 (MQ=255)

                                                                                                             ctctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTg                        <  1:1304370/140‑1 (MQ=255)

                                                                                                             ctctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTg                        >  2:2011777/1‑140 (MQ=255)

                                                                                                              tctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGt                       >  1:104037/1‑140 (MQ=255)

                                                                                                               ctAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTc                      >  2:1407076/1‑140 (MQ=255)

                                                                                                                tAATCCAGCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCa                     <  2:1885698/140‑1 (MQ=255)

                                                                                                                       gCAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag              <  1:1311849/140‑1 (MQ=255)

                                                                                                                        cAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagc             >  2:2450881/1‑140 (MQ=255)

                                                                                                                        cAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag              <  1:2429263/139‑1 (MQ=255)

                                                                                                                        cAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcag              >  2:2429263/1‑139 (MQ=255)

                                                                                                                        cAAAGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATAGCCAGCGCGTTACGGAGAGGGTCCTACCCGAGATTTGTCCCTTCcagc             >  2:1171770/1‑140 (MQ=255)

                                                                                                                         aaaGGAAAAAGTAGATCTCAGGCTCTCAGCGCGAAAACGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca            <  1:708845/140‑1 (MQ=255)

                                                                                                                         aaaGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAACATGATTGTCTTCCCTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca            <  2:1051634/140‑1 (MQ=255)

                                                                                                                         aaaGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca            <  1:1369213/140‑1 (MQ=255)

                                                                                                                         aaaGGAAAAAGTAGACCTCAGGCTCCCAGAGCGAAAACGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagca            <  1:678453/140‑1 (MQ=255)

                                                                                                                          aaGGAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCcagcag           >  1:1554626/1‑140 (MQ=255)

                                                                                                                            ggAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGat         <  1:1389839/140‑1 (MQ=255)

                                                                                                                            ggAAAAAGTAGAGCTCAAGGTCGAAGAGCGAAAATGGTTGTCTTCGCTTCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGat         <  2:908137/140‑1 (MQ=255)

                                                                                                                             gAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGatt        <  1:626350/140‑1 (MQ=255)

                                                                                                                             gAAAAAGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGatt        <  2:2045547/140‑1 (MQ=255)

                                                                                                                               aaaaGTAGAGCTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGattat      >  1:2247781/1‑140 (MQ=255)

TAGCCGAATCGACCCAGAATGTGTTTTGCCACTGTTTTTCTGGCCGCGTGGAAGTCACCTCTTCCTGCCAGACATTACACAGCACATTGCGACCCGCAATATTCAGCGTCTCTAATCCAGCAAAGGAAAAAGTAGAACTCAGGGTCGCAGAGCGAAAATGGTTGTCTTCACTCCAGAGAATATCGCGCGTCCAGCGGCTGCCATCTTTTATTGCCAGCGCGTTACGCAGAGGGTCCTGCCCGGAATTTGTCACTTCCAGCAGATTATTATT  >  NC_000913/1048771‑1049041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`