Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,146,273 A→G L108S (TTG→TCG)  yceF ← m(7)GTP pyrophosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,146,2730AG99.4% 553.8 / NA 158L108S (TTG→TCG) yceFm(7)GTP pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (64/92);  total (66/92)
Fisher's exact test for biased strand distribution p-value = 4.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

CTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAAT  >  NC_000913/1146138‑1146408
                                                                                                                                       |                                                                                                                                       
ctctTAAAGCTACCCGCGCAGTTCAGGGGATCCTCGTTATGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     <  2:990020/140‑1 (MQ=255)
ctctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     <  2:1654826/140‑1 (MQ=255)
ctctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     <  2:1469230/140‑1 (MQ=255)
ctctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     <  1:2371733/140‑1 (MQ=255)
ctctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     <  1:1440178/140‑1 (MQ=255)
 tctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGcc                                                                                                                                    >  1:1562231/1‑140 (MQ=255)
  ctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGcca                                                                                                                                   <  2:2244192/140‑1 (MQ=255)
  ctTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGcca                                                                                                                                   <  2:183495/140‑1 (MQ=255)
   ttAAAGCTACCCGCGTAGTGCACGGCATGTTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccag                                                                                                                                  <  1:1050889/140‑1 (MQ=255)
   ttAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTATTTGCAGCTGCTCAATCGCCGTATTAAACGACGccag                                                                                                                                  >  1:801499/1‑140 (MQ=255)
   ttAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGCCTCCACTTGTGTCTGCAGATGCCCACTCGCGGAATTAAACGACGccag                                                                                                                                  >  2:888797/1‑140 (MQ=255)
    tAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACTGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagt                                                                                                                                 <  2:376856/140‑1 (MQ=255)
    tAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagt                                                                                                                                 <  2:1249095/140‑1 (MQ=255)
     aaaGCTACCCGCGCATTGCAGGTGACGCTCTTTACGCCCGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  1:888797/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGTGACGCTATTTACGCTCGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  1:934124/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  1:1513352/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  2:271793/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  2:1234835/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  1:322028/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  1:240304/140‑1 (MQ=255)
     aaaGCTACCCGCGCAGTACAGGCGATGCTCTTTACGCACATAATTATCCATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtc                                                                                                                                <  2:801499/140‑1 (MQ=255)
      aaGCTACCCGCGCAGTGCAGGGTATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcc                                                                                                                               <  1:1110360/140‑1 (MQ=255)
      aaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcc                                                                                                                               <  1:234505/140‑1 (MQ=255)
      aaGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcc                                                                                                                               <  2:1676241/140‑1 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGGTGCCCATCCGCGGAGTTAAACGACGccagtccctgtc                                                                                                                          >  1:703226/1‑135 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcca                                                                                                                              >  2:484196/1‑136 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcca                                                                                                                              <  1:484196/136‑1 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGccagtcc                                                                                                                               >  2:2083242/1‑135 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     >  2:559021/1‑129 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGc                                                                                                                                     >  2:2421322/1‑129 (MQ=255)
           aCCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGtat                                                                                                                          >  1:60784/1‑140 (MQ=255)
              cgcgCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGa                                                                                                                       <  2:1562231/140‑1 (MQ=255)
              cgcgCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGa                                                                                                                       <  1:2137726/140‑1 (MQ=255)
              cgcgCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGa                                                                                                                       <  2:2440913/140‑1 (MQ=255)
                  cAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGt                                                                                                                   <  2:85051/140‑1 (MQ=255)
                  cAGTGCAGGGGATGCTCTTTAAGCACGTAATTATCACTCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGt                                                                                                                   <  2:2295632/140‑1 (MQ=255)
                      gCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACg                                                                                                               >  2:1667465/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTTGCCGAATTCAACAACGGCAGTCCAGGATAGGAGGTGACGATATTGCCg                                                                                                      >  2:690657/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTGAACCACGAAAGTCCAGAATAGGGGGTGACGATATTGCCg                                                                                                      >  2:1172679/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGcca                                                                                                      >  2:1605915/1‑139 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  2:1813943/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  2:1657231/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  2:2183132/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  2:1334357/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  2:2324951/1‑140 (MQ=255)
                               gCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCg                                                                                                      >  1:1770407/1‑140 (MQ=255)
                                     tACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCt                                                                                                <  2:326359/140‑1 (MQ=255)
                                      aCGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCtt                                                                                               <  1:2415674/140‑1 (MQ=255)
                                               aTTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAAtt                                                                                      >  1:2476268/1‑140 (MQ=255)
                                                    cAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGa                                                                                 <  2:60784/140‑1 (MQ=255)
                                                    cAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGa                                                                                 <  1:185572/140‑1 (MQ=255)
                                                      aTCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACg                                                                               >  1:1847358/1‑140 (MQ=255)
                                                       tctcCGCCTCGCTCATGTGACGCAACTGGTCGTCAAATGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACgg                                                                              <  1:690657/140‑1 (MQ=255)
                                                       tctcCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACgg                                                                              <  2:2476268/140‑1 (MQ=255)
                                                          ccGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCa                                                                           <  1:311236/140‑1 (MQ=255)
                                                           cGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACAATATTGCTGCTCGCCTTGCGTCATTGCAGACGGGCAt                                                                          >  1:972765/1‑140 (MQ=255)
                                                            gCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCAtt                                                                         >  2:151190/1‑140 (MQ=255)
                                                              cTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCAtttt                                                                       <  1:1501515/140‑1 (MQ=255)
                                                                cGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTttct                                                                     <  2:1929571/140‑1 (MQ=255)
                                                                       gTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTa                                                              >  2:384498/1‑140 (MQ=255)
                                                                        tGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTaa                                                             <  2:1743222/140‑1 (MQ=255)
                                                                          aCGGAAATAGACGTCACGAGGCACCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACg                                                           <  2:1155105/140‑1 (MQ=255)
                                                                           cGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACgg                                                          >  2:1913789/1‑140 (MQ=255)
                                                                              aaaTGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGttt                                                       <  1:166719/140‑1 (MQ=255)
                                                                               aaTGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCGGACTGGGATTTTCGTCCGTTAACGGTTTa                                                      >  1:1041870/1‑140 (MQ=255)
                                                                                 tGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTAcc                                                    >  1:1928882/1‑140 (MQ=255)
                                                                                           aaGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCg                                          <  1:2324951/140‑1 (MQ=255)
                                                                                           aaGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCg                                          <  1:384498/140‑1 (MQ=255)
                                                                                             ggCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGcc                                        <  2:1473775/140‑1 (MQ=255)
                                                                                             ggCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGcc                                        <  1:1982936/140‑1 (MQ=255)
                                                                                                tCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATc                                     >  1:1712467/1‑140 (MQ=255)
                                                                                                tCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGAATTATATTCCGTTAACTGTTTGTCGGTGATGTCGCCATc                                     >  2:1113475/1‑140 (MQ=255)
                                                                                                 ccACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCATTAACGATTTACCAGTGATTTCGCCATCa                                    >  1:813890/1‑140 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATTCCCAGTCGCCGAATTAACCGATGCCAGTCCTGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   <  2:752436/140‑1 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCGGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   <  1:160658/140‑1 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAATGGTTTACCGGTGATTTCGCCATCaa                                   <  1:12629/140‑1 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   >  1:553735/1‑140 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   <  1:2399878/140‑1 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCATTCGCCGAACTAAACGACGCCAGTCCTGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   <  2:680161/140‑1 (MQ=255)
                                                                                                  cACTTCTGTTTGCAGATGCCCAGTCGCCGAATTAACCGATGCCAGTCCGGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCaa                                   <  2:926863/140‑1 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCATTCGCTGAGTTATACGACGCTAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  1:1113475/140‑1 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  1:1667465/140‑1 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              >  2:1273545/1‑140 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  1:2183132/140‑1 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  1:1519422/140‑1 (MQ=255)
                                                                                                       cTGTTTGCAGATGCCCAATCGCCGAATTAAACGACCCCATTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  2:1041870/140‑1 (MQ=255)
                                                                                                        tGTTTGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCa                                    <  2:2325708/133‑1 (MQ=255)
                                                                                                          tttGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  2:1374592/137‑1 (MQ=255)
                                                                                                          tttGCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              >  1:1374592/1‑137 (MQ=255)
                                                                                                             gCAGATGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATAcc                        <  1:1273545/140‑1 (MQ=255)
                                                                                                             gCAGATGCCCATTCGCCCAATTAAACGACGCTAGTTCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATAcc                        <  2:1082707/140‑1 (MQ=255)
                                                                                                                 aTGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGt                    <  1:2065957/140‑1 (MQ=255)
                                                                                                                  tGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              <  1:1529786/129‑1 (MQ=255)
                                                                                                                  tGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAaca                              >  2:1529786/1‑129 (MQ=255)
                                                                                                                  tGCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTc                   >  2:1902906/1‑140 (MQ=255)
                                                                                                                   gCCCATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAATGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCt                  <  2:256884/140‑1 (MQ=255)
                                                                                                                    cccATTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTg                 <  1:1693208/140‑1 (MQ=255)
                                                                                                                       aTTCGCCGAATTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAAc              <  1:2484362/140‑1 (MQ=255)
                                                                                                                               aaTTAAACGACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAAtt      >  2:2092509/1‑140 (MQ=255)
                                                                                                                                 ttAAACGACGCCAGTGCAGTATAGATGGTGACGATATGGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTaa    <  1:1172679/140‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                       
CTCTTAAAGCTACCCGCGCAGTGCAGGGGATGCTCTTTACGCACGTAATTATCAATCTCCGCCTCGCTCAGGTGACGGAAATGGACGTCAAAAGGCTCCACTTCTGTTTGCAGATGCCCATTCGCCGAATTAAACAACGCCAGTCCAGTATAGAAGGTGACGATATTGCCGCTGGCTTTGCGTAATTGCAGACGGGCATTTTCTTCCGTTAACGGTTTACCGGTGATTTCGCCATCAAGAACACATACCTGGTCTGAACCAATAATTAAAT  >  NC_000913/1146138‑1146408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑