BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,190,955	A→G	R344R (CGT→CGC)	purB ←	adenylosuccinate lyase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,190,955	0	A	G	99.2%	410.1 / NA	119	R344R (CGT→CGC)	purB	adenylosuccinate lyase
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (71/46); total (73/46)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 121 total aligned reads displayed.

TGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACT  >  NC_000913/1190818‑1191084

tGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCg                                                                                                                                 >  1:1853865/1‑140 (MQ=255)

tGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGGAGATTGATGTGCGATCGAGGCATAAGCGATGCCCACGCCGAGGTTGCg                                                                                                                                 >  2:641516/1‑140 (MQ=255)

   aTTGGTTCAGCCAGCCCTTCCCCGTTGTGGTCCAGTTAATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCgcag                                                                                                                              <  1:830705/140‑1 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGGTTGATATGCAATCAAGCCATAACCGCTACCCACGCCGAGGTTGCgcag                                                                                                                              >  2:1021718/1‑140 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAGTCAAGGCATAAGCGATACCCAGGCCGAGGTTGCgcag                                                                                                                              >  1:881691/1‑140 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCgcag                                                                                                                              <  1:2006190/140‑1 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCgcag                                                                                                                              <  1:580220/140‑1 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCgcag                                                                                                                              <  2:1975156/140‑1 (MQ=255)

   aTTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCgcag                                                                                                                              <  1:2291031/140‑1 (MQ=255)

       gTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACg                                                                                                                          >  1:290784/1‑140 (MQ=255)

        ttCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACgg                                                                                                                         <  1:1710274/140‑1 (MQ=255)

        ttCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGATGCgcagc                                                                                                                             >  1:2469542/1‑136 (MQ=255)

          cagccagcACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTa                                                                                                                       >  2:1475788/1‑140 (MQ=255)

            gccagcACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTaga                                                                                                                     >  1:171437/1‑140 (MQ=255)

             ccagcACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATTCCCACGCCGAGGTTGCGCAGCACGGTagag                                                                                                                    <  1:1718880/140‑1 (MQ=255)

                 cACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAAGCGATACCAACGCCGAGATTGCGCAGCACGGTAGAGTCgg                                                                                                                >  2:1006184/1‑140 (MQ=255)

                 cACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCgg                                                                                                                >  2:2235187/1‑140 (MQ=255)

                 cACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCgg                                                                                                                <  2:2049631/140‑1 (MQ=255)

                 cACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCgg                                                                                                                >  2:2414603/1‑140 (MQ=255)

                 cACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCgg                                                                                                                >  2:1261490/1‑140 (MQ=255)

                 acagtCCCAGTTGTGATCCAGTTCATCCAGCAGATAGTCCCGGTTCACCTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCgg                                                                                                                <  2:957074/136‑1 (MQ=255)

                       ccAGTTGTGATCCAGTTCATCCAGCCGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCggtcaggt                                                                                                          <  1:1021718/140‑1 (MQ=255)

                       ccAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCggtcaggt                                                                                                          >  1:1815352/1‑140 (MQ=255)

                              tgATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTg                                                                                                   >  1:1833651/1‑140 (MQ=255)

                                aTCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                                                                                                 >  1:345967/1‑140 (MQ=255)

                                aTCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                                                                                                 >  2:1938568/1‑140 (MQ=255)

                                aTCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                                                                                                 >  2:48770/1‑140 (MQ=255)

                                aTCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACAGTAGAGTCGCTCAGGTCACGcctgt                                                                                                 >  1:957074/1‑136 (MQ=255)

                                aTCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGAGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGcc                                                                                                 >  1:612241/1‑140 (MQ=255)

                                     gTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg                                                                                            >  2:432332/1‑140 (MQ=255)

                                     gTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg                                                                                            >  1:2397331/1‑140 (MQ=255)

                                     gTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg                                                                                            >  2:2194557/1‑140 (MQ=255)

                                     gTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTCCGCAGCATGGTAGAGTCGGTCAGGTCACGCTGCCAGCgg                                                                                            >  2:1014105/1‑140 (MQ=255)

                                     gTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCATGGTAGAGTAGGTCAGGTCACGCTGCCAGCgg                                                                                            >  2:705497/1‑140 (MQ=255)

                                      ttCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCggg                                                                                           >  1:2125193/1‑140 (MQ=255)

                                          tCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAAc                                                                                       >  1:500912/1‑140 (MQ=255)

                                           ccAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAAcc                                                                                      >  2:485188/1‑140 (MQ=255)

                                             agcagATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTCCCACGGTGGATTGATATGCAGTCAAGGTATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAActgc                                                                                    <  2:1410274/140‑4 (MQ=255)

                                               cagATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCa                                                                                  >  2:2413351/1‑140 (MQ=255)

                                                agATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAg                                                                                 >  1:1566946/1‑140 (MQ=255)

                                                    ggTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTttg                                                                             >  1:393797/1‑140 (MQ=255)

                                                    ggTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTttg                                                                             >  2:1257182/1‑140 (MQ=255)

                                                    ggTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGACTCGGTCATGTCACGCTGCCAGGGGGAAACCGGCAGTttg                                                                             >  2:957709/1‑140 (MQ=255)

                                                     gTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTttgc                                                                            >  2:1459381/1‑140 (MQ=255)

                                                          ggTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGcc                                                                       <  1:2017884/140‑1 (MQ=255)

                                                           gTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCTGCAGTTTGCTTGCCa                                                                      <  1:2180923/140‑1 (MQ=255)

                                                           gTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCa                                                                      <  1:2235187/140‑1 (MQ=255)

                                                            ttCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAg                                                                     >  1:203767/1‑140 (MQ=255)

                                                             tCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGa                                                                    <  1:589349/140‑1 (MQ=255)

                                                             tCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGa                                                                    <  1:2246650/140‑1 (MQ=255)

                                                             tCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGa                                                                    <  2:264998/140‑1 (MQ=255)

                                                               aCTTCCAGTTTGCTCCCGCCTTCCAGGGTAGATTGATGTGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGAtg                                                                  <  1:1006184/140‑1 (MQ=255)

                                                               aCTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGAtg                                                                  <  2:290784/140‑1 (MQ=255)

                                                               aCTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGAtg                                                                  <  1:2414603/140‑1 (MQ=255)

                                                                cTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGAtgc                                                                 >  1:1634702/1‑140 (MQ=255)

                                                                      gTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGTCAGATGCTGCAAt                                                           >  1:2344869/1‑140 (MQ=255)

                                                                          gCTCACGCCTTTCAGTGTAGATAGACATGCAATCAAGCCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACcg                                                       <  1:641516/140‑1 (MQ=255)

                                                                          gCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACcg                                                       >  1:1005974/1‑140 (MQ=255)

                                                                                 ccTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGa                                                <  1:1938568/140‑1 (MQ=255)

                                                                                 ccTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGa                                                <  2:171437/140‑1 (MQ=255)

                                                                                 ccTTTCAGGGTGGATTGATATGCAAACAAAGCATAACAGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGa                                                <  1:1025119/140‑1 (MQ=255)

                                                                                  cTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGaa                                               <  1:1475788/140‑1 (MQ=255)

                                                                                     tCAGGGTGGATTGATTTGCAATAAATGCAGAACCGATCCCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAgg                                            <  1:782138/140‑1 (MQ=255)

                                                                                     tCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGGAGTGTGCTTGCCAGATTCTGCAATACCGCGTTGGAAAgg                                            >  2:782138/1‑140 (MQ=255)

                                                                                     tCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAgg                                            <  2:1853865/140‑1 (MQ=255)

                                                                                     tCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGACACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCCCGTTGGAAAgg                                            >  1:1867480/1‑140 (MQ=255)

                                                                                     tCAGGGTGGATTGATATGCAACCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAgg                                            >  1:463388/1‑140 (MQ=255)

                                                                                      cAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGc                                           <  2:1253766/140‑1 (MQ=255)

                                                                                          gTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCTGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAg                                       >  2:1567899/1‑140 (MQ=255)

                                                                                            ggATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGAt                                     >  2:13492/1‑140 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCCCGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTc                                   >  2:1943688/1‑140 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGGCAGATGCAGCAGTATCGCGTTGGAAAGGCCCAGATTc                                   >  1:653711/1‑140 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGaa                                               >  1:1459208/1‑128 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGaa                                               <  2:1459208/128‑1 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAg                                             >  2:504534/1‑130 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTc                                   >  2:207610/1‑140 (MQ=255)

                                                                                              aTTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTc                                   >  2:418782/1‑140 (MQ=255)

                                                                                                   tatGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTc                              >  2:2072197/1‑140 (MQ=255)

                                                                                                   tatGCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTc                              >  1:1322470/1‑140 (MQ=255)

                                                                                                      gCAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGa                           <  2:2094867/140‑1 (MQ=255)

                                                                                                       cAATCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAg                          >  1:1356749/1‑140 (MQ=255)

                                                                                                        aaTCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGt                         >  2:333627/1‑140 (MQ=255)

                                                                                                        aaTCAAGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTACCAGATGCCGCAATACCCCGTTGGAGAGGCGCAGATTCCCTTCGGAGt                         >  2:962356/1‑140 (MQ=255)

                                                                                                            aaGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATCCCGCGTTGGAAAGGGCCAGATTCCCTTCGGAGTTTTc                     >  1:989348/1‑140 (MQ=255)

                                                                                                            aaGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACTGCGTTGGAAAGGTCCAGTTTCCCTTCGGAGTTTTc                     >  2:893898/1‑140 (MQ=255)

                                                                                                            aaGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTc                     >  2:1808414/1‑140 (MQ=255)

                                                                                                            aaGGCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTc                     >  1:317453/1‑140 (MQ=255)

                                                                                                              ggCATAACCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGa                   <  1:485188/140‑1 (MQ=255)

                                                                                                                    aCCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGa             <  1:1954163/140‑1 (MQ=255)

                                                                                                                    aCCGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGa             <  1:48770/140‑1 (MQ=255)

                                                                                                                     ccGATACCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGAt            <  2:1005974/140‑1 (MQ=255)

                                                                                                                          aCCCACGCCGAGGTTGCGCAGCTCGCTAGAGTCGGTCCGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGt       <  1:1014105/140‑1 (MQ=255)

                                                                                                                          aCCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGt       <  2:345967/140‑1 (MQ=255)

                                                                                                                          aCCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGt       <  1:2413351/140‑1 (MQ=255)

                                                                                                                          aCCCACGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGt       >  1:333017/1‑140 (MQ=255)

                                                                                                                          aCCCACGCCGAGGTTGCACAGCCCGGTAGAGCCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGt       <  2:777870/140‑1 (MQ=255)

                                                                                                                               cGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACt  >  1:1658755/1‑140 (MQ=255)

                                                                                                                               cGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACt  >  1:79069/1‑140 (MQ=255)

                                                                                                                               cGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACt  >  2:2484766/1‑140 (MQ=255)

                                                                                                                               cGCCGAGGTTGCGCAGCACGGTAGAGTCGGTCAAGTCACGCTGCCCGCGGGAAACCGTCAATTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCAAGAATCCGTTCGGAGTTTTCGGAGGCGATCGGGGTAACt  >  2:635141/1‑140 (MQ=255)

TGGATTGGTTCAGCCAGCACTTCCCAGTTGTGATCCAGTTCATCCAGCAGATGGTCACGGTTCACTTCCAGTTTGCTCACGCCTTTCAGGGTGGATTGATATGCAATCAAGGCATAACCGATACCCACGCCGAGGTTACGCAGCACGGTAGAGTCGGTCAGGTCACGCTGCCAGCGGGAAACCGGCAGTTTGCTTGCCAGATGCTGCAATACCGCGTTGGAAAGGCCCAGATTCCCTTCGGAGTTTTCGAAGTCGATCGGGTTAACT  >  NC_000913/1190818‑1191084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`