Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,336,475	C→T	T215T (ACC→ACT)	acnA →	aconitate hydratase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,336,475	0	C	T	98.4%	206.1 / NA	64	T215T (ACC→ACT)	acnA	aconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (27/35);  minor base G (1/0);  total (29/35)
Fisher's exact test for biased strand distribution p-value = 4.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAA  >  NC_000913/1336341‑1336610                                                                                                                                       |                                                                                                                                        aGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCAt                                                                                                                                    <  2:280559/140‑1 (MQ=255) aGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCAt                                                                                                                                    <  2:116803/140‑1 (MQ=255)    gtcgtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGAGTGCTTATCCGGAGACAGTCGTTGGTACTGACTCGCACACTACCATGAt                                                                                                                                 >  2:991405/1‑140 (MQ=255)       gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCaa                                                                                                                              <  1:2398497/140‑1 (MQ=255)       gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCaa                                                                                                                              <  2:357104/140‑1 (MQ=255)       gtGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCaa                                                                                                                              >  2:1459175/1‑140 (MQ=255)       gtGCCGCCAGGCACACGCATTTACCATCACGTTAACCACGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCaa                                                                                                                              <  1:1115711/140‑1 (MQ=255)        tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAAc                                                                                                                             >  1:315704/1‑140 (MQ=255)        tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAAc                                                                                                                             >  2:42210/1‑140 (MQ=255)        tGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAAc                                                                                                                             >  2:1402078/1‑140 (MQ=255)            gccAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGcc                                                                                                                         <  2:1426633/140‑1 (MQ=255)            gccAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGcc                                                                                                                         <  2:2183861/140‑1 (MQ=255)                    caggcaTTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTg                                                                                                                 <  1:24550/140‑1 (MQ=255)                           ttGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCtggggt                                                                                                          >  1:2332785/1‑140 (MQ=255)                           ttGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCtggggt                                                                                                          <  1:2470012/140‑1 (MQ=255)                           ttGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACCCGCGCACGACCATGATCAACGGCCTTGGCGTGCtggggt                                                                                                          >  2:760766/1‑140 (MQ=255)                            tGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCtggggtg                                                                                                         <  2:294624/140‑1 (MQ=255)                                 tCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACTCTATCATCATCAACGGACTTGCCGTGCTGGGGTGGGGCg                                                                                                    >  2:633926/1‑140 (MQ=255)                                 tCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCg                                                                                                    <  2:1313528/140‑1 (MQ=255)                                 tCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACAACCATGAACAAGGGGCTTGGCCTGCTGGGGTGGGGCg                                                                                                    >  1:858524/1‑140 (MQ=255)                                        aaCCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTtggtgg                                                                                             <  1:2422476/140‑1 (MQ=255)                                             cGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCg                                                                                        >  2:1677333/1‑140 (MQ=255)                                               aaTATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAAGGGCGTTGACGTGCTGGGGTGGGGCGTTGGTGGGATCGaa                                                                                      >  2:845059/1‑140 (MQ=255)                                               aaTATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGaa                                                                                      >  1:209738/1‑140 (MQ=255)                                                   tctcGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTCGGCGTGCGGGGCTGAGGCGTTGGTGGGATCGAAGCAg                                                                                  >  1:848024/1‑140 (MQ=255)                                                    ctcGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAg                                                                                  >  1:2173940/1‑139 (MQ=255)                                                    ctcGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAg                                                                                  <  2:2173940/139‑1 (MQ=255)                                                     tcGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGaa                                                                                <  1:2295435/140‑1 (MQ=255)                                                         cAAAGCAGTGTGGAGTGAATTGTAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCg                                                                            <  1:991405/140‑1 (MQ=255)                                                            aGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCaa                                                                         >  2:147063/1‑140 (MQ=255)                                                              cAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTAGGGTGGGTCGTTGGTGCGATCTAAGCAGAAGCCGCAATg                                                                       >  2:807138/1‑140 (MQ=255)                                                                 tgtgGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTa                                                                    >  1:126866/1‑140 (MQ=255)                                                                 tgtgGAGTGAATTGCAGGACGGCGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTa                                                                    >  1:1850447/1‑140 (MQ=255)                                                                    ggAGTGAATTGCAGGACGGTGACTGCATTACTTATCCAGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGc                                                                 <  1:807138/140‑1 (MQ=255)                                                                      aGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCATTGGTGGAATCGAATCAGAAGGCGCAATGTTAGGCCa                                                               >  1:806919/1‑140 (MQ=255)                                                                        tGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGc                                                             <  1:2212474/140‑1 (MQ=255)                                                                        tGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGc                                                             >  1:2379319/1‑140 (MQ=255)                                                                           aTTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGTGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCgg                                                          >  1:545265/1‑140 (MQ=255)                                                                           aTTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCgg                                                          >  2:1671034/1‑140 (MQ=255)                                                                               cAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTc                                                      >  1:165275/1‑140 (MQ=255)                                                                                aGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTcc                                                     <  1:42210/140‑1 (MQ=255)                                                                                   aCGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATg                                                  <  2:83368/140‑1 (MQ=255)                                                                                     ggTGAATGGATTGCTTATCCGGCTACACTCGTTGGTACTAACTCGCACGCTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCt                                                <  2:858524/140‑1 (MQ=255)                                                                                     ggTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCt                                                >  1:1925674/1‑140 (MQ=255)                                                                                      gTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCtt                                               <  2:1850447/140‑1 (MQ=255)                                                                                      gTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCtt                                               <  2:209738/140‑1 (MQ=255)                                                                                       tGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGGAATATTAGGCCAGCCGGTTTCCATGCTTa                                              >  1:877748/1‑140 (MQ=255)                                                                                       tGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTa                                              >  1:2205660/1‑140 (MQ=255)                                                                                       tGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCAGCAATGTTAGGCCAGCCGGTTTCCATGCTTa                                              >  2:785621/1‑140 (MQ=255)                                                                                        gAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGTGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTAt                                             <  2:545265/140‑1 (MQ=255)                                                                                        gAATGGATTGCTTATCCGGATACACTCGTTGCTACGGACTCGCACACTCCCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTAt                                             <  2:877748/140‑1 (MQ=255)                                                                                          aTGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATcc                                           <  2:2379319/140‑1 (MQ=255)                                                                                          aTGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATcc                                           <  2:417948/140‑1 (MQ=255)                                                                                             gATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCgg                                        <  1:441590/140‑1 (MQ=255)                                                                                                    tATCCGGATACACTCGTTGGTAGTGTCTCTCACTCTATCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGt                                 <  1:1150695/140‑1 (MQ=255)                                                                                                    tATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGt                                 <  1:1557659/140‑1 (MQ=255)                                                                                                    tATCCGGATACACTCGTTGGTACTGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGt                                 <  1:2312116/140‑1 (MQ=255)                                                                                                                           tGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACt                     >  1:1914136/1‑129 (MQ=255)                                                                                                                           tGACTCGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACt                     <  2:1914136/129‑1 (MQ=255)                                                                                                                                cGCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGt     <  1:2458084/140‑1 (MQ=255)                                                                                                                                 gCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTg    <  1:1677333/140‑1 (MQ=255)                                                                                                                                 gCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTg    >  2:194568/1‑140 (MQ=255)                                                                                                                                 gCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTg    <  1:194568/140‑1 (MQ=255)                                                                                                                                 gCACACTACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTg    <  1:785621/140‑1 (MQ=255)                                                                                                                                   acacTACCATGATCAGCGGCCTCGGCGTGGTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGaa  <  1:845059/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        AGCGTCGTGCCGCCAGGCACAGGCATTTGCCATCAGGTTAACCTCGAATATCTCGGCAAAGCAGTGTGGAGTGAATTGCAGGACGGTGAATGGATTGCTTATCCGGATACACTCGTTGGTACTGACTCGCACACCACCATGATCAACGGCCTTGGCGTGCTGGGGTGGGGCGTTGGTGGGATCGAAGCAGAAGCCGCAATGTTAGGCCAGCCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAA  >  NC_000913/1336341‑1336610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑